Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 15,410 | 0 | T | G | 41.7% | 112.5 / 10.5 | 60 | intergenic (+112/‑35) | dnaJ/insL1 | chaperone Hsp40, DnaK co‑chaperone/IS186 transposase |
Reads supporting (aligned to +/- strand): ref base T (4/31); new base G (25/0); total (29/31) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 4.57e-13 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTT‑AAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTC‑TGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGG > NC_000913/15263‑15530 | ggTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTT‑AAGGGTtgt < 1:719963/151‑1 (MQ=255) gtgAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTT‑AAGGGTTGTgg < 2:383479/150‑1 (MQ=255) gtgAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGT‑AAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTT‑AAGGGTTGTGGTa < 2:287577/151‑1 (MQ=255) gaagaaGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACACCCCCAAGAGCAAACTT‑TAGGGGTGTGGGAt > 1:334653/1‑151 (MQ=255) gaagaaGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGCAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGGCACTCTCATAAGGGCTAACTT‑AAGGGGTGTggga > 2:967089/1‑148 (MQ=255) agaagTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTT‑AAGGGTTGTGGTATTa < 1:19967/151‑1 (MQ=255) gTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGGTACTCCCACAAAAGCTAAACT‑AAGGGGTggggggttaaggc > 2:1191922/1‑142 (MQ=255) tttttGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGGCACTCCCCTAAGCGCTAACTT‑TAGGGGTGTGGGATTACGCCTg > 1:814925/1‑151 (MQ=255) tttGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGACACTCCCCTAAGCGCCAACTT‑AAGGGGTGTGGTATTACGCCCGat > 2:1203842/1‑151 (MQ=255) tttGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTT‑AAGGGTTGTGGTATTACGCCTGa < 2:718641/150‑1 (MQ=255) ttGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGGCACTCCCATAAAAGGTAAATT‑AAAGGGTGTggggttttgcctggt > 2:358681/1‑137 (MQ=255) gacgacCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGGCGCTTCCAAAAACGCCAACTT‑TAGGGGTGTGGGTTTACGACTggtttt > 2:839733/1‑146 (MQ=255) gacgacCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTT‑AAGGGTTGTGGTATTACGCCTGATATg < 2:319252/151‑1 (MQ=255) gacgacCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTT‑AAGGGTTGTGGTATTACGCCTGATATg < 1:1030536/151‑1 (MQ=255) acgacCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTT‑AAGGGTTGTGGTATTACGCCTGATATGa < 2:254265/151‑1 (MQ=255) acCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTT‑AAGGGTTGTGGTATTACGCCTGATATGAttt < 1:736244/151‑1 (MQ=255) gACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGTCACTCCCCTAAGCGCTAACTT‑AAAGGGTGTGGTATTACGCCTGGTATGAtttta > 1:835689/1‑147 (MQ=255) gACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGAGCACTCCCCCAAACGCTAACTT‑AAGGGGTTTGGGATTAAGCCTGATATGATTTaa > 2:175229/1‑149 (MQ=255) ccGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGGCACTCCCCCAAGCGCCTACTT‑TAAGGGTGTGGGATTACGCCCGGTATTATTTTAAGTGc > 2:593181/1‑151 (MQ=255) aaCCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTT‑AAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATg < 2:29080/151‑1 (MQ=255) aaaaGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTT‑AAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTActct < 2:425702/151‑1 (MQ=255) aaGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATAAATCCCCCAAACGCCAAATT‑TAAGGGTGGGGGATTACGCCTGGTATGATTTTAAGTGCCGATGAATTaatttcc > 2:54190/1‑145 (MQ=255) aGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTT‑AAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCAc < 1:910756/151‑1 (MQ=255) gCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGGCACTCCCATAAACGCTAACTT‑AAAGGGTGTGGGATTATGCCTGAGATTATTTTACATGCCCGTGAAATAATCTCAcc > 2:965632/1‑150 (MQ=255) gCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTT‑AAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACg < 2:139825/151‑1 (MQ=255) cTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGAGGCGGGGAAAACTCCCCAAAGCGCTAATTT‑AAAGGGTGTGGTCTTACGCCTTCTATTATTTTAAGTGGCGATTAATTACTTTCACGa > 1:487194/1‑150 (MQ=255) gCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTT‑AAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATa < 1:1232032/150‑1 (MQ=255) gTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGAGCACTCCCATAAGCGCTAAATT‑TAGGGGTGTGGGATTTTGCCTGATTTTGTTTTAAGGGGCGAAGAATTAATCTCTCGATAAATgg > 2:783970/1‑151 (MQ=255) gggCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGTCACTCCCCTAAACGCTAACTT‑AAGGGGTGTGGGATTTCGCCTGATATGATTTTACGTGCCGATGAATTACTCTCCCGATAAATGGTc > 1:1044021/1‑151 (MQ=255) gggCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGTCACTCCCATAAACGCCCACTT‑AAAGGGTGTGGGGTTTCGCCTCATATTATTTTAAGTGCCGATGAATTACTCTCCCGATAAATGGTc > 1:114960/1‑151 (MQ=255) gCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTT‑AAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAg < 1:829684/151‑1 (MQ=255) cAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTT‑AAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGc < 1:295397/151‑1 (MQ=255) cAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTT‑AAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGc < 1:14023/151‑1 (MQ=255) ccTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTT‑AAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAAtt < 2:398991/151‑1 (MQ=255) gggTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCCTAAAAGCCAACTT‑TATGGTTTTTGTGGTAGTCCTTGAATTATTTAACGTGCCGGTGAATTACTCTCACCATAAATgggtaatgattttc > 1:216257/1‑139 (MQ=255) aaaaTAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGAGGACTCCCATAAACGCTAACTT‑AAGGGGTGTGGGGTTATGCCTGATATGATTTAACGTGCCGATGAAATACTTTCACGATAAATGGTCAGCAATTCTTGGccc > 2:286605/1‑151 (MQ=18) aTAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTT‑AAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTC‑TGGCCCatat < 2:1418337/151‑1 (MQ=255) aTAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAAATT‑AAGGGGTGTGGGATTACGCCTGATATGATTTTACGTGCCGATGAATTAATCTCACGATAACTGGTCAGCAATTC‑TTGcccctct > 1:587136/1‑147 (MQ=18) aGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGTCACTCCCCTAAAAGCCAACTT‑AAAGGGGGTGGGATTTTGGCTGATATAATTTTAAACGCCGATTGAATACTCTTCCCATAACAGGTCAGCAAATT‑TGGCCCATATTg > 2:1232607/1‑151 (MQ=255) aGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTT‑AAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTC‑TGGCCCATAtt < 2:334658/150‑1 (MQ=255) gggTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTT‑AAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTC‑TGGCCCATATTgg < 1:1230773/151‑1 (MQ=255) gCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTT‑AAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTC‑TGGCCCATATTGGTAAg < 2:288971/151‑1 (MQ=255) gCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAACGCCAAATT‑TAGGGGTGTGGGATTTAGCCTGATATGATTTTAAGTGCCGGTGAAATACTCTCACGATAAATGGTCAGCAATTC‑TTGcccccatttgtta > 1:404174/1‑140 (MQ=255) gTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGGCACTCCCCTAAGCGCTAAATTAAAGGGTGTTGGTTTTA‑GCCCGCTATGATTTTAAGTGCCGATGAAATACTCTCCACATAAATTGTTAGCAATTT‑TGGCCCATATTGGGAAggc > 1:1219264/1‑149 (MQ=255) gtGAAGATATGCGATCACCGGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTT‑AAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATAACTCTCACGATAACTGGTCAGCAATTC‑TGGCCCATATTGGTAAGccc < 2:1240712/150‑1 (MQ=255) aGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCCTAAACGCTAACTT‑AAGGGGTGTGGGATTACGCCTGATATGATTTAAACTGCCGGTGAATTACTCTCCCGATAACTGGTCAGCAATTC‑TGGCCCATATTTGTAAACCCga > 1:731781/1‑149 (MQ=17) aGATATGCGAGCACCTGTAAAGTGGCGGGGATCAATCCCATAAGCGGTAACTT‑AAGGGGTGGGGGGTTAAGCCCGATATGGTTTAAAGTGCCGGTGAAATTATTTCCCCGTAACTGGGCAGCAAATT‑TGGGCCCTATTTGTAAGCCCga > 2:911316/1‑149 (MQ=255) tGCGAGCACCTGTAAAGTGGCGGGGGGCACCCCCATAAGCGCTGACTT‑TAAGGGTGTGGGTTTTAGCCCGGTATTATTTTACGGGCCGATGAATTATTTTCACGATAAATGGGTAGCAATTT‑TTGGCCATATTTGTAAAACCGAAGAACTg > 2:192091/1‑151 (MQ=255) gCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTTACTT‑AAGGGGTGGGGTATTAAGCCTGATATGATTTAAAGTGGCGATGAATTAATCTCACGAAAACTGGTCAGCAATTC‑TTGCCCATATTGGTAAGCCCGAAGAAATgg > 1:720446/1‑151 (MQ=17) gAGCACCTGTAAAGTGGCGGGGGTCACTCCCCTAAACGCTCACTT‑AAAGGGTGTGGGATTACGCCCGGTATGATTTAAAGTGGCGATGAATTACTCTCCCCATAAATTGTCAGCAATTT‑TGGCCCCTATTTGTTAGACCCCAGAAATGGAt > 2:245991/1‑151 (MQ=255) gAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTT‑AAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTC‑TGGCCCATATTGGTAAGCCCGAAGAACTGGAt < 1:1296590/151‑1 (MQ=255) cACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTT‑AAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTC‑TGGCCCATATTGGTAAGCCCGAAGAACTGGATACt < 2:750907/151‑1 (MQ=255) ccTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTT‑AAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTC‑TGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTc < 2:488320/151‑1 (MQ=255) aaaGTGGCGGGGATCACTCCCATAAGCGCTAACTT‑AAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTC‑TGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCAc < 1:221310/151‑1 (MQ=39) aGTGGCGGGGATCACTCCCATAAGCGCTAACTT‑AAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTC‑TGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGt < 1:166263/151‑1 (MQ=37) gTGGCGGGGATCACTCCCATAAGCGCTAACTT‑AAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTC‑TGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTa < 1:358760/151‑1 (MQ=37) ggCGGGGATCACTCCCATAAGCGCTAACTT‑AAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTC‑TGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTaa < 1:859569/150‑1 (MQ=35) cGGGGATCACTCCCATAAGCGCTAACTT‑AAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTC‑TGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGc < 2:390899/151‑1 (MQ=33) ggggATCACTCCCATAAGCGCTAACTT‑AAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTC‑TGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGcc > 2:44862/1‑151 (MQ=33) ggATCACTCCCATAAGCGCTAACTT‑AAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTC‑TGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCgg > 2:1439086/1‑151 (MQ=33) | GGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTT‑AAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTC‑TGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGG > NC_000913/15263‑15530 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |