Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,435,7270GA42.6% 147.9 / 78.9 116intergenic (‑108/+33)uspF/ompNstress‑induced protein, ATP‑binding protein/outer membrane pore protein N, non‑specific
Reads supporting (aligned to +/- strand):  ref base G (56/10);  new base A (0/49);  total (56/60)
Fisher's exact test for biased strand distribution p-value = 7.14e-23
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 118 total aligned reads displayed.

GAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAG‑ATCAAAGGAGGAAGAATAAGCAGAAAAAG‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATG  >  NC_000913/1435579‑1435877
                                                                                                                                                     |                                                                                                                                                       
gAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAG‑ATCAAAGGAGGAAGAATAAGCAg                                                                                                                                                         <  2:385226/149‑1 (MQ=255)
gAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAG‑ATCAAAGGAGGAAGAATAAGCAGa                                                                                                                                                        <  1:1020769/150‑1 (MQ=255)
gAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAG‑ATCAAAGGAGGAAGAATAAGCAGa                                                                                                                                                        <  1:91735/150‑1 (MQ=255)
  aTCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAG‑ATCAAAGGAGGAAGAATAAGCAGaaa                                                                                                                                                      >  1:153774/1‑150 (MQ=255)
    cGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAG‑ATCAAAGGAGGAAGAATAAGCAGaaaa                                                                                                                                                     >  1:540741/1‑149 (MQ=255)
      gAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAG‑ATCAAAGGAGGAAGAATAAGCAGAAAAAG‑cc                                                                                                                                                <  1:212852/151‑1 (MQ=255)
        aaTATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAG‑ATCAAAGGAGGAAGAATAAGCAGAAAAAG‑CCCg                                                                                                                                              >  1:1227037/1‑151 (MQ=255)
        aaTATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAG‑ATCAAAGGAGGAAGAATAAGCAGAAAAAG‑CCCg                                                                                                                                              >  1:54357/1‑151 (MQ=255)
            tCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAG‑ATCAAAGGAGGAAGAATAAGCAGAAAAAG‑CCCGCCAt                                                                                                                                          >  2:782484/1‑151 (MQ=255)
             cGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAG‑ATCAAAGGAGGAAGAATAAGCAGAAAAAG‑CCCGCCAt                                                                                                                                          >  2:1031641/1‑150 (MQ=255)
              gATAGGGACAAGAATCGTTCTGTTAATAAACCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAG‑ATCAAAGGAGAAAGAATAAGCAGAAAAAG‑CCCGCCATaa                                                                                                                                        <  2:231700/151‑1 (MQ=255)
               aTAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAG‑ATCAAAGGAGGAAGAATAAGCAGAAAAAG‑CCCGCCATAAc                                                                                                                                       >  2:565852/1‑151 (MQ=255)
                tAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAG‑ATCAAAGGAGGAAGAATAAGCAGAAAAAG‑CCCGCCATAAc                                                                                                                                       >  2:272440/1‑150 (MQ=255)
                 aGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAG‑ATCAAAGGAGGAAGAATAAGCAGAAAAAG‑CCCGCCATAAc                                                                                                                                       >  1:405137/1‑149 (MQ=255)
                      aaaGATTGGTTCTTTTCAAAAACCCTCGTGTTGAAGTACCCGAAAAAAGTGAACCCCAAGATACCATTTTTGGTGTAATGCGGGAGTCCTCATTTTTAATTTAA‑ATAAAGGAAAAAAAAAAAAGGAAAAAAAG‑CCCGCCATAACAGCGGGc                                                                                                                                <  2:505322/150‑1 (MQ=255)
                       aaGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAG‑ATCAAAGGAGGAAGAATAAGCAGAAAAAG‑CCCGCCATAACAGCGGGc                                                                                                                                >  1:1004297/1‑150 (MQ=255)
                         gAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAG‑ATCAAAGGAGGAAGAATAAGCAGAAAAAG‑CCCGCCATAACAGCGGGCagg                                                                                                                             >  2:473595/1‑151 (MQ=255)
                              gTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAG‑ATCAAAGGAGGAAGAATAAGCAGAAAAAG‑CCCGCCATAACAGCGGGCAGGAGGAt                                                                                                                        >  1:878329/1‑151 (MQ=255)
                              gTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAG‑ATCAAAGGAGGAAGAATAAGCAGAAAAAG‑CCCGCCATAACAGCGGGCAGGAGGAt                                                                                                                        >  2:1102365/1‑151 (MQ=255)
                               ttCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAG‑ATCAAAGGAGGAAGAATAAGCAGAAAAAG‑CCCGCCATAACAGCGGGCAGGAGGAtt                                                                                                                       >  1:1167718/1‑151 (MQ=255)
                                 tttttCAAAAACCCTCTTTTTTTAGTATCCGAATAAGTTTTAACGCCAGAGCACTCTTTTTGGATAATGACGGATTTCGCTTTTTAAATTTAA‑ACAAAAGGAGAAAAAATAAGCAAAAAAAT‑CCCGCCATAACAGCGGGCAGGAGGAttt                                                                                                                      <  1:154654/147‑1 (MQ=255)
                                 ttGTTAAAAAAACCTCTTTTTTTAGTATCCGCATAAAGTGAACCGCCAGATGACATTTTTTGTGTAAGGACGGAGTCCACATTTTTAATTAAG‑AACAAAGGAGAAAAAATAAGCAAAAAAAG‑CCCGCCATAACAGCGGGCAGGAGGAttt                                                                                                                      <  2:687949/149‑1 (MQ=255)
                                   gTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAG‑ATCAAAGGAGGAAGAATAAGCAGAAAAAG‑CCCGCCATAACAGCGGGCAGGAGGAttt                                                                                                                      >  1:969784/1‑148 (MQ=255)
                                   gTTCAAAAACCCTCTTTTTTTATTATCCGAAAAAAGTGTACCGCCAGATGACATTTTTTGTGTAAGGACGGAGTTCACTTTTTTAATTTAG‑ATCAAAGGAGAAAAAAAAAGCAGAAAAAG‑CCCGCCATAACAGCGGGCAGGAGGAttt                                                                                                                      <  1:1419450/148‑1 (MQ=255)
                                        tAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAG‑ATCAAAGGAGGAAGAATAAGCAGAAAAAG‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTg                                                                                                               >  2:624195/1‑150 (MQ=255)
                                          aaccctcttTTTTTATTATCCAAATAAAGTGAAGCGCGAGATGAAATTTTTTGTAAAACGAGGGAGTTATTATTTTT‑ATTTAGAAAAAAAGAAGGAAAAATAGCAAAAAAAAC‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATa                                                                                                            <  1:729451/148‑1 (MQ=255)
                                          aaccctcttGTTTTAGTATCCGAAAAAAGTGTACCGCCAGATGACATTTTTTGTGTAATGCCGGATTTAACTTTTTTAATTAAG‑AACAAAGGAGGAAGAAAAAGAAAAAAAAG‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATa                                                                                                            <  1:779914/148‑1 (MQ=255)
                                           aaCCTACTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAG‑ATCAAAGGCGGAAGAATAAGCAGAAAAAG‑CCCGCCATAACAGCGGGCAGGAGGCTTTAGAACTGATa                                                                                                            >  2:611006/1‑150 (MQ=255)
                                                cctTTTTTATTATCCGCAAAAAGTTAAACGCCAGAGGACATTTTTTGTGTAATGACGGATTTCATTTTTTAAATTTAG‑ACAAAAGGAGAAAAAAAAAGCAAAAAACC‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAg                                                                                                      <  2:294471/151‑1 (MQ=255)
                                                cctTTTTTAGTTTCAATATAAAGTATACCGCCAGAATACACTTTTTGTAAAAAGACGGAGTACAAATTTTTAATTTAA‑ACAAAAGGAGAAAAAATAACAAAAAAAAC‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAAcc                                                                                                        <  1:931691/149‑1 (MQ=255)
                                                cctTTTTTAGTATCCGAAAAAAGTGAAACGCAAGATGACATTTTTTGTGTAATGACGGATTTCACTTTTTTAATTTAG‑ATAAAAGGAGAAAAAAAAAGAAAAAAACG‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAg                                                                                                      <  2:513791/151‑1 (MQ=255)
                                                cctTTTTTAGTACCCGAAAAAAGTGTAACCCCAGATGACATTTTTTGTGAAATGACGGAGTTCACATTTTTAATTAAG‑ACAAAAGGAGGAAAAATAAGAAAAAAAAG‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAg                                                                                                      <  2:516955/151‑1 (MQ=255)
                                                    ttttAGTATCCGCATAAAGTGTAACGCCAGATGACTTTTTTTGTGTAATGACGGATTTCACATTTTAAATTTAG‑ATAAAAGGAGAAAAAAAAAGAAAAAAAAG‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGAcc                                                                                                   <  1:1403455/150‑1 (MQ=255)
                                                      ttAGTATCCGTATAAAGTGTAACGCAAGAGGACCTTTTTTGTATAATGACGGATTTCACATTTTTATTTGAG‑AACAAAGGAGAAAAAAAAAACAAAAAAGC‑CCCGCCACAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTa                                                                                                 <  2:1172888/150‑1 (MQ=255)
                                                        aGTATCCGAATAAAGTCCAACGCCAGAGAACATTTTTTGTGTAACGACGGCTTTCAAATTTTTAATTTAG‑CAAAAGGGAGAAAAAAAAAGCAAAAAACG‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAg                                                                                              <  2:1227041/151‑1 (MQ=255)
                                                        aGTACCCGCATAAAGTGTACCGCCAGATGACTCTTTTTGTGTAATGACGGATTTCACTTTTTTAATTTAG‑ATAAAAGGAGGAAAAAAAAGCAAAAAAAG‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAg                                                                                              <  1:1222843/151‑1 (MQ=255)
                                                           aTCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAG‑ATCAAAGGAGGAAGAATAAGCAGTAAAAG‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCg                                                                                            >  2:431177/1‑150 (MQ=255)
                                                           aTCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAG‑ATCAAAGGAGGAAGAATAAGCAGAAAAAG‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCg                                                                                            >  1:585519/1‑150 (MQ=255)
                                                            tCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAG‑ATCAAAGGAGGAAGAATAAGCAGAAAAAG‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGAc                                                                                          >  1:698665/1‑151 (MQ=255)
                                                              cGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAG‑ATCAAAGGAGGAAGAATAAGCAGAAAAAG‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACa                                                                                         >  1:107085/1‑150 (MQ=255)
                                                               gCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAG‑ATCAAAGGAGGAAGAATAAGCAGAAAAAG‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAat                                                                                       >  2:135076/1‑151 (MQ=255)
                                                                 aTAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAG‑ATCAAAGGAGGAAGAATAAGCAGAAAAAG‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAatat                                                                                     >  2:14617/1‑151 (MQ=255)
                                                                  tAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAG‑ATCAAAGGAGGAAGAATAAGCAGAAAAAG‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATatc                                                                                    >  2:1111772/1‑151 (MQ=255)
                                                                   aaaGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAG‑ATCAAAGGAGGAAGAATAAGCAGAAAAAG‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATatc                                                                                    >  2:401618/1‑150 (MQ=255)
                                                                   aaaGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAG‑ATCAAAGGAGGAAGAATAAGCAGAAAAAG‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATatc                                                                                    >  1:1190976/1‑150 (MQ=255)
                                                                      gtgtAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAG‑ATCAAAGGAGGAAGAATAAGCAGAAAAAG‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCg                                                                                >  2:442005/1‑151 (MQ=255)
                                                                          aaCGCCAGATGACCTTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAG‑ATAAAAGGAGAAAAAAAAAGAAAAAAAAG‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTag                                                                            <  1:40656/151‑1 (MQ=255)
                                                                          aaCGCCAGATGACATTTTTTGTGTAAGGACGGAGTTCATTTTTTTAATTTAG‑ATCAAAGGAGGAAAAAAAAGAAAAAAAAG‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTag                                                                            <  1:125179/151‑1 (MQ=255)
                                                                          aaCGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAG‑ATCAAAGGAGGAAGAATAAGCAGAAAAAG‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGt                                                                              >  2:1055105/1‑149 (MQ=255)
                                                                          aaCGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAG‑ATCAAAGGAGGAAGAATAAGCAGAAAAAG‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGt                                                                              >  2:956175/1‑149 (MQ=255)
                                                                              ccAAATGCCACTTTTTGTGTAATGACGGATTCCACTTTTTAAATTTAG‑AACAAAGGAGGAAGAAAAAGAAAAAAAGG‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGAt                                                                        <  2:314856/151‑1 (MQ=255)
                                                                               cAGATGACTTTTTTTGTGTAATGACGGATTTCACATTTTAAATTAAG‑ATCAAAGGAGAAAAAAAAAGCAGAAAAAG‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATg                                                                       <  1:204350/151‑1 (MQ=255)
                                                                                aGATGACCTTTTTTGTGTAATGAGGGATTTCACTTTTTTAATTAAG‑AAAAAGGGAGAAAAAAAAAGAAAAAAAAG‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGc                                                                      <  2:749260/151‑1 (MQ=255)
                                                                                 gATGACTCTTTTGGTTTAAGGAGGGAGTTCAAATTTTAAATTTAA‑AAAAAGGGAGGAAGAAAAAGCAAAAAAAG‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGcc                                                                     <  1:675230/151‑1 (MQ=255)
                                                                                    gactttttttGTGTAATGAGGGAGTTCACATTTTTAATTTAG‑ATAAAAGGAGAAAAAAAAAGAAAAAAAAG‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCAtt                                                                  <  1:57061/146‑1 (MQ=255)
                                                                                    gACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAG‑ATCAAAGGAGGAAGAATAAGCAGAAAAAG‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCAtt                                                                  >  1:1337596/1‑151 (MQ=255)
                                                                                        ttttttGTGTAATGACGGATTTCACATTTTTAATTAAA‑ATAAAAGGAGGAAAAAAAAGCAAAAAAAG‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGc                                                               <  1:1139413/149‑1 (MQ=255)
                                                                                        ttttttGGATAATGAGGGAGTTAACTTTTTAAATTTAG‑AAAAAAGGAGAAAGAAAAAGAAAAAAAGG‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGc                                                               <  1:14764/149‑1 (MQ=255)
                                                                                         tttttGTATAAGGAGGGATTTCAC‑TTTTTAATTTTA‑AACAAAGGGAGAAAAAAAAAAAAAAAAAGCCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGc                                                               <  2:25494/149‑1 (MQ=255)
                                                                                             tgtgTAATGACGGATTTCACTTTTTTAATTAAG‑ACAAAAGAAGAAAAAAAAAGAAAAAAAAG‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGt                                                          <  1:1426900/150‑1 (MQ=255)
                                                                                              gtgtAATGACGGAGTTCACATTTTTAATTTAA‑ATAAAAGGAGGAAAAAAAAGCAAAAAAAG‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAg                                                        <  1:1022551/151‑1 (MQ=255)
                                                                                                gtAATGAGGGATTTCACATTTTAAATTAAG‑AAAAAAGGAGAAAAAAAAAGAAAAAAAAC‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGa                                                       <  2:1436767/150‑1 (MQ=255)
                                                                                                 tAATGACGGAGTTCACATTTTTAATTTAG‑ATCAAAGGAGGAAGAATAAGCAGAAAAAG‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGa                                                       >  2:863611/1‑149 (MQ=255)
                                                                                                     gACGGAGTTCACATTTTTAATTTAG‑ATCAAAGGAGGAAGAATAAGCAGAAAAAG‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGt                                                 >  2:545804/1‑151 (MQ=255)
                                                                                                     gACGGAGTTCACATTTTTAATTTAG‑ATCAAAGGAGGAAGAATAAGCAGAAAAAG‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGt                                                 >  2:33397/1‑151 (MQ=255)
                                                                                                      aCGGAGTTCACTTTTTTAATTTAA‑ATAAAAGGAGAAAGAAAAAGAAAAAAAAG‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAAAAAGAAAAAAAGAGAAAAGAGAAGCGGTAGAGAGGCCATTGGCAGCGTAGAAGCTGTc                                                <  1:1468194/151‑1 (MQ=255)
                                                                                                       cGGAGTTCACATTTTTAATTTAG‑ATCAAAGGAGGAAGAATAAGCAGAAAAAG‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTc                                                >  1:645896/1‑150 (MQ=255)
                                                                                                        ggAGTTCACATTTTTAATTTAG‑ATCAAAGGAGGAAGAATAAGCAGAAAAAG‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCAt                                              >  2:180225/1‑151 (MQ=255)
                                                                                                          aGTTCACATTTTTAATTTAG‑AACAAAGGAGAAAAAATAAGCAAAAAAAG‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCt                                            <  1:47544/151‑1 (MQ=255)
                                                                                                               acaTTTTTAATTTAG‑ATCAAAGGAGGAAGAATAAGCAGAAAAAG‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATc                                       >  2:1334289/1‑151 (MQ=255)
                                                                                                                 aTTTTTAATTTAG‑ATCAAAGGAGGAAGAATAAGCAGAAAAAG‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATcc                                      >  2:1343130/1‑150 (MQ=255)
                                                                                                                   ttttAATTTAG‑ATCAAAGGAGGAAGAATAAGCAGAAAAAG‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAAc                                   >  1:249871/1‑151 (MQ=255)
                                                                                                                     ttaattaaG‑AAAAAAGGAGAAAAAAAAAGCAAAAAAAG‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACa                                  <  2:969774/150‑1 (MQ=255)
                                                                                                                      tAATTTAG‑ATCAAAGGAGGAAGAATAAGCAGAAAAAG‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAgg                                >  1:920142/1‑151 (MQ=255)
                                                                                                                      tAATTAAG‑ATAAAAGGAGGAAGAAAAAGAAAAAAAAG‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAg                                 <  1:548453/150‑1 (MQ=255)
                                                                                                                       aaTTTAG‑ATCAAAGGAGGAAGAATAAGCAGAAAAAG‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAggg                               >  1:356821/1‑150 (MQ=255)
                                                                                                                       aaTTTAG‑ATCAAAGGAGGAAGAATAAGCAGAAAAAG‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGt                               >  1:169098/1‑151 (MQ=255)
                                                                                                                        aTTTAG‑ATCAAAGGAGGAAGAATAAGCAGAAAAAG‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGtt                              >  1:454162/1‑151 (MQ=255)
                                                                                                                        aTTAAG‑ATAAAAGGAGAAAAAAAAAGAAAAAAAAG‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGtt                              <  2:365715/151‑1 (MQ=255)
                                                                                                                         tttAG‑ATCAAAGGAGGAAGAATAAGCAGAAAAAG‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTg                             >  2:1297654/1‑151 (MQ=255)
                                                                                                                           tgaaaaaaaaGGAGGAAGAATAAGCAGAAAAAG‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTg                             <  1:1059534/143‑1 (MQ=255)
                                                                                                                            aG‑ATCAAAGGAGGAAGAATAAGCAGAAAAAG‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGAtt                          >  2:406939/1‑151 (MQ=255)
                                                                                                                              gacaaaaGGAGGAAGAAAAAGAAAAAAAAG‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGAttt                         <  1:288926/147‑1 (MQ=255)
                                                                                                                               agcAAAGGAGAAAAAAAAAGCAAAAAACC‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGAtttt                        <  1:54263/149‑1 (MQ=255)
                                                                                                                                 aaaaGGAGAAAGAAGAAGAACAAAAAG‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTtat                      <  1:161424/150‑1 (MQ=255)
                                                                                                                                   aaGGAGAAAAAAAAAAAAAAAAAAC‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAg                    <  2:757494/151‑1 (MQ=255)
                                                                                                                                    aggaggAGAAAAAAGAAAAAAAGG‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGt                   <  1:777401/151‑1 (MQ=255)
                                                                                                                                    aggaggAAAAAAAAGAAAAAAAAG‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGt                   <  2:456154/151‑1 (MQ=255)
                                                                                                                                    aggagaAAAAAAAAGAAAAAAAAG‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGt                   <  1:436952/151‑1 (MQ=255)
                                                                                                                                     ggagaaAGAAAAAGCAAAAAAAC‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTc                  <  2:1004291/151‑1 (MQ=255)
                                                                                                                                      gagaaAAAAAAAGAAAAAAAAG‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCa                 <  1:604728/151‑1 (MQ=255)
                                                                                                                                            gaaTAAGCAGAAAAAG‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGt             >  2:403778/1‑149 (MQ=255)
                                                                                                                                            aaaTAAGAAGAAAAAG‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAg           <  1:272436/150‑1 (MQ=255)
                                                                                                                                             aaTAAGAAAAAAAAG‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAgg          <  1:624197/151‑1 (MQ=255)
                                                                                                                                              aaaaGCAAAAAAAG‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGt         <  1:1032116/149‑1 (MQ=255)
                                                                                                                                              aaaaGCAAAAAAAG‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGt         <  1:1402467/149‑1 (MQ=255)
                                                                                                                                              aTAAGCAGAAAAAG‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGt         >  2:378707/1‑151 (MQ=255)
                                                                                                                                               tAAGCAGAAAAAG‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTg        >  1:707053/1‑151 (MQ=255)
                                                                                                                                                   cAGAAAAAG‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGAc     >  1:381130/1‑150 (MQ=255)
                                                                                                                                                     gAAAAAG‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATg  >  2:571654/1‑151 (MQ=255)
                                                                                                                                                     |                                                                                                                                                       
GAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAG‑ATCAAAGGAGGAAGAATAAGCAGAAAAAG‑CCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATG  >  NC_000913/1435579‑1435877

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑