Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,622,8160GA44.1% 81.9 / 115.5 111intergenic (+75/+144)yneM/dgcZinner membrane‑associated protein/diguanylate cyclase, zinc‑sensing
Reads supporting (aligned to +/- strand):  ref base G (59/3);  new base A (0/49);  total (59/52)
Fisher's exact test for biased strand distribution p-value = 2.40e-28
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 113 total aligned reads displayed.

TTTTATGGCCGTACTGGGAATAATTTTATTTTCTGGTTTTCTGGCCGCGTATTTCAGCCACAAA‑TGGGATGACTAATGAACGGAGATAATCCCTCACCTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTATGACGGCGAAAAAAAA‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGTTTTCTCAATGAATGTTAAACGGAGCTTAAACT  >  NC_000913/1622666‑1622966
                                                                                                                                                         |                                                                                                                                                       
ttttATGGCCGTACTGGGAATAATTTTATTTTCTGGTTTTCTGGCCGCGTATTTCAGCCACAAA‑TGGGATGACTAATGAACGGAGATAATCCCTCACCTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTATGACGGCg                                                                                                                                                         >  2:1224376/1‑151 (MQ=255)
  ttATGGCCGTACTGGGAATAATTTTATTTTCTGGTTTTCTGGCCGCGTATTTCAGCCACAAA‑TGGGATGACTAATGAACGGAGATAATCCCTCACCTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTATGACGGCGa                                                                                                                                                        >  1:774049/1‑150 (MQ=255)
  ttATGGCCGTACTGGGAATAATTTTATTTTCTGGTTTTCTGGCCGCGTATTTCAGCCACAAA‑TGGGATGACTAATGAACGGAGATAATCCCTCACCTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTATGACGGCGa                                                                                                                                                        <  1:1232249/150‑1 (MQ=255)
   tATGGCCGTACTGGGAATAATTTTATTTTCTGGTTTTCTGGCCGCGTATTTCAGCCACAAA‑TGGGATGACTAATGAACGGAGATAATCCCTCACCTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTATGACGGCGaa                                                                                                                                                       >  2:204670/1‑150 (MQ=255)
   tATGGCCGTACTGGGAATAATTTTATTTTCTGGTTTTCTGGCCGCGTATTTCAGCCACAAA‑TGGGATGACTAATGAACGGAGATAATCCCTCACCTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTATGACGGCGaa                                                                                                                                                       >  2:597633/1‑150 (MQ=255)
    aTGGCCGTACTGGGAATAATTTTATTTTCTGGTTTTCTGGCCGCGTATTTCAGCCACAAA‑TGGGATGACTAATGAACGGAGATAATCCCTCACCTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTATGACGGCGaaa                                                                                                                                                      <  1:730224/150‑1 (MQ=255)
      ggCCGTACTGGGAATAATTTTATTTTCTGGTTTTCTGGCCGCGTATTTCAGCCACAAA‑TGGGATGACTAATGAACGGAGATAATCCCTCACCTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTATGACGGCGaaaaa                                                                                                                                                    >  2:6839/1‑150 (MQ=255)
      ggCCGTACTGGGAATAATTTTATTTTCTGGTTTTCTGGCCGCGTATTTCAGCCACAAA‑TGGGATGACTAATGAACGGAGATAATCCCTCACCTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTATGACGGCGaaaaa                                                                                                                                                    >  1:68771/1‑150 (MQ=255)
          gTACTGGGAATAATTTTATTTTCTGGTTTTCTGGCCGCGTATTTCAGCCACAAA‑TGGGATGACTAATGAACGGAGATAATCCCTCACCTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTATGACGGCGAAAAAAAA‑cc                                                                                                                                              >  1:981903/1‑151 (MQ=255)
          gTACTGGGAATAATTTTATTTTCTGGTTTTCTGGCCGCGTATTTCAGCCACAAA‑TGGGATGACTAATGAACGGAGATAATCCCTCACCTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTATGACGGCGAAAAAAAA‑cc                                                                                                                                              >  2:573311/1‑151 (MQ=255)
               gggAATAATTTTATTTTCTGGTTTTCTGGCCGCGTATTTCAGCCACAAA‑TGGGATGACTAATGAACGGAGATAATCCCTCACCTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTATGACGGCGAAAAAAAA‑CCGcc                                                                                                                                           <  2:295393/149‑1 (MQ=255)
               gggAATAATTTTATTTTCTGGTTTTCTGGCCGCGTATTTCAGCCACAAA‑TGGGATGACTAATGAACGGAGATAATCCCTCACCTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTATGACGGCGAAAAAAAA‑CCGcc                                                                                                                                           >  2:1149330/1‑149 (MQ=255)
                 gAATAATTTTATTTTCTGGTTTTCTGGCCGCGTATTTCAGCCACAAA‑TGGGATGACTAATGAACGGAGATAATCCCTCACCTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTATGACGGCGAAAAAAAA‑CCGCCAGt                                                                                                                                        >  1:1372756/1‑150 (MQ=255)
                   ataatTTTATTTTCTGGTTTTCGGGCCGGGTATTTCAGCCACAAA‑TGGGATGACTAATGAACGGAGATAATCCCTCACCTAACCGGACCC‑TTGTTACAGTTGTGTACAAGGGGAA‑TGATTTTTATGACGGCGAAAAAAAA‑CCGCCAGTaa                                                                                                                                      >  2:337079/1‑150 (MQ=255)
                           attttCTGGTTTTCTGGCCGCGTATTTCAGCCACAAA‑TGGGATGACTAATGAACGGAGATAATCCCTCACCTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTATGACGGCGAAAAAAAA‑CCGCCAGTAAACCGGCGGt                                                                                                                             >  1:170838/1‑151 (MQ=255)
                           attttCTGGTTTTCTGGCCGCGTATTTCAGCCACAAA‑TGGGATGACTAATGAACGGAGATAATCCCTCACCTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTATGACGGCGAAAAAAAA‑CCGCCAGTAAACCGGCGGt                                                                                                                             >  1:997579/1‑151 (MQ=255)
                             tttctggttttctggCCGCGTATTTCAGCCACAAA‑TGGGATGACTAATGAACGGAGATAATCCCTCACCTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTATGACGGCGAAAAAAAA‑CCGCCAGTAAACCGGCGGTg                                                                                                                            >  2:1148652/1‑150 (MQ=255)
                              ttctggttttctggCCGCGTATTTCAGCCACAAA‑TGGGATGACTAATGAACGGAGATAATCCCTCACCTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTATGACGGCGAAAAAAAA‑CCGCCAGTAAACCGGCGGTGa                                                                                                                           >  2:378076/1‑150 (MQ=255)
                               tttggttttctgcCCGATTTTTCCAGCCAAAAT‑TGGAATGACAAATAAAAGGAAATATTCCCCCCCCAACCCGCCCCC‑TTGTTACAGTTGTGTACAGGGGCCT‑TAATTTTTATGAGGGCAAAAAAAAA‑CCGCCAGTAAACCGGCGGTGaa                                                                                                                          <  2:1056449/148‑1 (MQ=255)
                               tctggttttctggCCGCGTATTTCAGCCACAAA‑TGGGATGACTAATGAACGGAGATAATCCCTCACCTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTATGACGGCGAAAAAAAA‑CCGCCAGTAAACCGGCGGTGAAt                                                                                                                         >  1:964486/1‑151 (MQ=255)
                               tctggttttctggCCGCGTATTTCAGCCACAAA‑TGGGATGACTAATGAACGGAGATAATCCCTCACCTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTATGACGGCGAAAAAAAA‑CCGCCAGTAAACCGGCGGTGAAt                                                                                                                         >  2:827/1‑151 (MQ=255)
                               tctggttttctggCCGCGTATTTCAGCCACAAA‑TGGGATGACTAATGAACGGAGATAATCCCTCACCTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTATGACGGCGAAAAAAAA‑CCGCCAGTAAACCGGCGGGGAAt                                                                                                                         >  1:910887/1‑151 (MQ=255)
                                 tgtttttctggCCGCTTATTTCACCCAAAAA‑GGGGATGATTAAGGACGGGAAATAACCCCTCACTTACCCGCCCCT‑TTTTTACATTTGTGTAAAGGGGGCT‑TGTTTTTTATGACGGAAAAAAAAAA‑CCGCCAGTAAACCGGCGGTGAATg                                                                                                                        <  1:720994/147‑1 (MQ=255)
                                    ttttcgggCCGCTTTTTCCACCCAAAAA‑GGGGATGACTAAGAAAGGGAGATAACCCCCCCCCTACCCGCCCCT‑TTTTTACTTTTGTGTACAGGGGCCT‑TGTTTTTTAGGACGGAAAAAAAAAA‑CCGCCAGTAAACCGGCGGTGAATGCtt                                                                                                                     <  1:33670/150‑1 (MQ=255)
                                     tttctggCCGCGTATTTCAGCCACAAA‑TGGGATGACTAATGAACGGAGATAATCCCTCACCTAACCGGCCCC‑TTGTCACAGTTGTGTACAAGGGGCC‑TGATTTTTATGACGGCGAAAAAAAA‑CCGCCAGTAAACCGGCGGTGAATGCTTGc                                                                                                                   >  2:699204/1‑151 (MQ=255)
                                      ttctggCCGCTTTTTCCAGCCAAAAA‑GGGAATGACAAAGGAAGGGAAAAAACCCCTCCCCTAACCGCCCCT‑TTGTTACGGTGGTGAAAAGGGGGCT‑TGTTTTTTAGGAGGGAAAAAAAAAA‑CCGCCAGTAAACCGGCGGTGAATGCTTGc                                                                                                                   <  2:550056/150‑1 (MQ=255)
                                      ttcgggCCGCTTTTTCCACCCAAAAG‑GGGGATGACTAAGGAGGGGAAATAACCCCCCCCCAACCCGCCCCT‑TTGTAACGGTTGTGTACAGGGGGCT‑TATTTTTTAGGAGGGGAAAAAAAAC‑CCGCCAGTAAACCGGCGGTGAATGCTTGc                                                                                                                   <  2:120253/150‑1 (MQ=255)
                                      ttcggcccGCTTATTCCACCCAAAAA‑GGGGATGATTAATGAGCGGAGATAACCCCCCCCCTACCCGCCCCT‑TTGTTCCGGTTGTGAAAAGGGGGCT‑TGTTTTTTATGACGGAAAAAAAAAA‑CCGCCAGTAAACCGGCGGTGAATGCTTGc                                                                                                                   <  2:1426373/144‑1 (MQ=255)
                                          gcccGCGTATTCCAGCCACAAG‑TGGGAGGAAAAAGGACGGAAAACAGCCCCTCTCCAATCCCCCCTC‑TTGTCACTGTTGTTAAAAAGGGTCC‑TGTTTTTGATGGCGGAAAAAAAAAC‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATgg                                                                                                               <  2:216147/148‑1 (MQ=255)
                                            ccGCTTATTT‑AGCCAAAAAGTGGGTTAATAAAAGAGCGGAGAAACCCCCTCCCCTACCCGCCCCT‑TTTTTAGATTGGTAAACAGGGGTCG‑TTTTTTTTTTCACGGAAAAAAAAAA‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATa                                                                                                            <  2:854493/151‑1 (MQ=255)
                                             cgcgTATTCCAGCCAAAAA‑TGGGATGACAAATGAACGGAGATAATCCCCCACCAAACCGGCCCT‑TTGTTACAGTTGTGTACAGGGGGCC‑TGTTTTTTATGGCGGCAAAAAAAAA‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAg                                                                                                           <  1:884354/151‑1 (MQ=255)
                                                gTATTTCAGCCACAAA‑TGGGATGACTAATGAACGGAGATAATCCCTCACCTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTATGACGGCGAAAAAAAA‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGAtt                                                                                                        >  1:1255087/1‑151 (MQ=255)
                                                  tttccAGCCAAAAA‑GGGGATGAATAATGAAGGGAAATAACCCCCCCCCTACCCGGCCCT‑TTGTTACGTTTGTGTACAAGGGGCC‑TGTTTTTTATGCCGGAAAAAAAAAA‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGAttt                                                                                                       <  1:1226022/146‑1 (MQ=255)
                                                    ttcagatccaAA‑TGGGATGACTAATGAACGGAGATAACCCCTCACCTAACCGGCCGC‑TTGTTATCGTTGTGTACAAGGGGCC‑TGATTTTTATGACGCCGAAAAAAGA‑CCGCCAGTAAACCGGCGGAGAATGCTTGCATGGATAGATTggtg                                                                                                    >  2:177752/9‑151 (MQ=255)
                                                        gCCACAAA‑TGGGATGACTAATGAACGGAGATAATCCCTCACCTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTATGACGGCGAAAAAAAA‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGtttt                                                                                                >  2:1420655/1‑151 (MQ=255)
                                                            caAA‑TGGGATGACTAATGAACGGAGATAATCCCTCACCTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTATGACGGCGAAAAAAAA‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCtt                                                                                            >  1:942717/1‑151 (MQ=255)
                                                              aaagggggttaatAAAGAACGGAAAAAACCCCCCCCCCAACCCGCCCCTTTTTTACTTTTTTGTAAAGGGGGCT‑TTTTTTTTAGGCGGGAAAAAAAAAA‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCtt                                                                                            <  2:1115962/139‑1 (MQ=255)
                                                               a‑TGGGATGACTAATGAACGGAGATAATCCCTCACCTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTATGACGGCGAAAAAAAA‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGGTTTGCtttt                                                                                          >  2:216585/1‑150 (MQ=255)
                                                                  gggATGACTAATGAACGGAGATAATCCCTCACCTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTATGACGGCGAAAAAAAA‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACg                                                                                       >  1:1043306/1‑151 (MQ=255)
                                                                  gggATGACAAATGAACGGAAATACCCCCCCCCCTACCCGCCCCT‑TTTTTACATTTGTGTAAAGGGGGCT‑TGTTTTTTATGAGGGAAAAAAAAAA‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACg                                                                                       <  1:406066/151‑1 (MQ=255)
                                                                    gATGACTAATGAACGGAGATAATCCCTCACCTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTATGACGGCGAAAAAAAA‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCt                                                                                     >  1:474828/1‑151 (MQ=255)
                                                                       gactaaggagggaaaaTAACCCCTCCCCTACCCGCCCCT‑TTTTTACATTTGTGTAAAGGGGGCT‑TGTTTTTTATGACGGAAAAAAAAAA‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAAc                                                                                  <  1:1253688/136‑1 (MQ=255)
                                                                         aaaaTGAAGGGAAAAAACCCCCCCCCTACCCGCCCCT‑TTTTTACGTTTGTGTAAAGGGGGCT‑TTTTTTTTATGAGGGGAAAAAAAAA‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAg                                                                                <  2:1179985/149‑1 (MQ=255)
                                                                            aTGAACGGAGATAATCCCTCACCTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTATGACGGCGAAAAAAAA‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGc                                                                              >  1:714780/1‑150 (MQ=255)
                                                                              gAACGGAAATACCCCCTCCCCAACCCGCCCCC‑TTGTTACGTTTGTGTACAAGGGGCC‑TTTTTTTTAGGGCGGAAAAAAAAAG‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCAtt                                                                           <  2:766095/151‑1 (MQ=255)
                                                                                cgggAGATAACCCCTCCCCTACCCGGCCCT‑TTTTTACAGTTGTGTAAAAGGGGCT‑TGTTTTTTATGACGGCAAAAAAAAA‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCAtttt                                                                         <  1:269256/149‑1 (MQ=255)
                                                                                 cGGAGATAATCCCTCACCTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTATGACGGCGAAAAAAAA‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGGGTTTTGCTTTTACGCTAACAGGCATTTTc                                                                        >  1:1032955/1‑151 (MQ=255)
                                                                                       aactccctcccCAAACCGCCCCT‑TTTTTATAGTTGTGTAAAGGGGGCT‑TTTTTTTTAGGAGGGAAAAAAAAAG‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCAc                                                                  <  2:578633/148‑1 (MQ=255)
                                                                                        aaTCCCTCACCTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTATGACGGCGAAAAAAAA‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACt                                                                 >  2:1124353/1‑151 (MQ=255)
                                                                                          tCCCTCACCTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTATGACGGCGAAAAAAAA‑CCGCCAGTAAACCGGCGGTGCATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGAATTTTCCTGCACTg                                                                >  2:1346397/1‑150 (MQ=255)
                                                                                           ccccccccaacccGCCCCT‑TTTTTACATTTGTTAACAGGGGGCT‑TTTTTTTTAGGCGGGAAAAAAAAAA‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGAt                                                              <  2:980710/140‑1 (MQ=255)
                                                                                           cccTCACCTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTATGACGGCGAAAAAAAA‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGAt                                                              >  1:7973/1‑151 (MQ=255)
                                                                                           cccTCACCTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTATGACGGCGAAAAAAAA‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGAt                                                              >  1:1234594/1‑151 (MQ=255)
                                                                                            ccacACCAACCCGGCCTG‑TGGTTAAATTTGTTTACAGGGGCCT‑TTTTTTTTTTGACGGAAAAAAAAAA‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATa                                                             <  2:581070/148‑1 (MQ=255)
                                                                                            ccTCACCTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTATGACGGCGAAAAAAAA‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGAt                                                              >  2:1436817/1‑150 (MQ=255)
                                                                                              cccccTACCCGCCCCT‑TTTTTACGGTTGTTAAAAGGGGGCT‑TGTTTTTTAGGGCGGAAAAAAAAAA‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAAc                                                           <  2:528295/148‑1 (MQ=255)
                                                                                              cccccTACCCGCCCCT‑TTTTTAATTTTGTGTAAAAGGGCCT‑TGTTTTTAATGGCGCAAAAAAAAAA‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAAc                                                           <  1:426347/148‑1 (MQ=255)
                                                                                               ccccTACCCGCCCCT‑TTGTTCCATTTTTGAAAAAGGGCCC‑TG‑TTTTGACGGGGGGAAAAAAAAAGCCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAAc                                                           <  1:204665/148‑1 (MQ=255)
                                                                                                aCCTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTATGACGGCGAAAAAAAA‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACg                                                          >  2:1364682/1‑150 (MQ=255)
                                                                                                 ccTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTATGACGGCGAAAAAAAA‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGa                                                         >  2:717273/1‑150 (MQ=255)
                                                                                                   tAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTATGACGGCGAAAAAAAA‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATc                                                      >  1:786288/1‑151 (MQ=255)
                                                                                                    acccGCCCCT‑TTGTTACAGTTGTGTAAAGGGGGCT‑TGTTTTTTAGGCCGGAAAAAAAAAA‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATc                                                      <  1:502215/148‑1 (MQ=255)
                                                                                                    acccGCCCCC‑TTTTTACATTTGTG‑AAAAGGGGCCTTGTTTTTTAGGCCGGAAAAAAAAAA‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCg                                                     <  1:284655/149‑1 (MQ=255)
                                                                                                    aaCCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTATGACGGCGAAAAAAAA‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCg                                                     >  1:96033/1‑151 (MQ=255)
                                                                                                       cGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTATGACGGCGAAAAAAAA‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTg                                                  >  2:1346817/1‑151 (MQ=255)
                                                                                                         cccct‑TTTTTACAGTTGTGTACAGGGGGCT‑TGTTTTTTAGGACGGAAAAAAAAAA‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGac                                                <  1:274692/150‑1 (MQ=255)
                                                                                                         cccct‑TTGTTACAGTTGTGAAAAGGGGGCT‑TTTTTTTTATGACGGAAAAAAAAAA‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGac                                                <  1:406074/150‑1 (MQ=255)
                                                                                                          cccc‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTATGACGGCGAAAAAAAA‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGac                                                >  2:678067/1‑150 (MQ=255)
                                                                                                            cctttGTTAGGGTGGTGTACAGGGGGCT‑TTTTTTTTATGAGGGAAAAAAAAAA‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGacac                                              <  2:769344/148‑1 (MQ=255)
                                                                                                             c‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTATGACGGCGAAAAAAAA‑CCGCCAGTAAACCGGCGGGGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGacac                                              >  1:995384/1‑149 (MQ=255)
                                                                                                                tGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTATGACGGCGAAAAAAAA‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGt                                           >  1:695576/1‑150 (MQ=255)
                                                                                                                  ttACAGTTGTGTACAAGGGGCC‑TGATTTTTATGACGGCGAAAAAAAA‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGc                                        >  2:360050/1‑151 (MQ=255)
                                                                                                                    acatttgttaaaaggggcCC‑TGTTTTTTAGGCCGGAAAAAAAAAA‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCa                                       <  1:1191047/136‑1 (MQ=255)
                                                                                                                    aCAGTTGTGTACAAGGGGCC‑TGATTTTTATGACGGCGAAAAAAAA‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCAt                                      >  1:547/1‑151 (MQ=255)
                                                                                                                     gaGTTGTATAAAGGGGGCC‑TGATTTTTATGACGGAAAAAAAAAA‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATc                                     <  1:1032329/150‑1 (MQ=255)
                                                                                                                         tgtgTACAAGGGGCC‑TGATTTTTATGACGGCGAAAAAAAA‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGtt                                 >  1:40772/1‑151 (MQ=255)
                                                                                                                          gtgtAAAGGGGGCT‑TTTTTTTTAGGGCGGAAAAAAAAAA‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGttt                                <  1:359620/151‑1 (MQ=255)
                                                                                                                           tgtACAAGGGGCC‑TGATTTTTATGACGGCGAAAAAAAA‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGtttt                               >  1:682824/1‑151 (MQ=255)
                                                                                                                           tgtACAAGGGGCC‑TGATTTTTATGACGGCGAAAAAAAA‑CCGCCAGTAAACCGGCGGAGAATGCTTGCATGGATAGGTTTGTGGTTTGCTTTTACGCTAACAGGCATTTTCCCGCACTGATAAAGAATCGTTGTCACAGTAGCATCAGtttt                               >  1:88920/1‑151 (MQ=255)
                                                                                                                              aCAAGGGGCC‑TGATTTTTATGACGGCGAAAAAAAA‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGTTTtctc                            >  1:631093/1‑151 (MQ=255)
                                                                                                                                 gggcgCC‑TTATTTTTATGCGGACAAAAAAAAA‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGTTTTCTCaat                         <  1:1149326/147‑1 (MQ=255)
                                                                                                                                  ggggCT‑TGTTTTTTATGCCGGAAAAAAAAAA‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGTTTTCTCaatg                        <  1:1224369/151‑1 (MQ=255)
                                                                                                                                  ggggCC‑TTTTTTTTAGGACGGAAAAAAAAAA‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGTTTTCTCaatg                        <  1:406651/151‑1 (MQ=255)
                                                                                                                                   gggCT‑TTATTTTTATGCGGGAAAAAAAAAA‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGTTTTNNCaatga                       <  2:1259002/151‑1 (MQ=255)
                                                                                                                                    gggctttttttttATGCGGGCAAAAAAAAA‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGTTTTCTCaatga                       <  2:484534/143‑1 (MQ=255)
                                                                                                                                       c‑TGATTTTTATGACGGCGAAAAAAAA‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGTTTTCTCAATGAATGt                   >  1:715086/1‑151 (MQ=255)
                                                                                                                                       c‑TGATTTTTATGACGGCGAAAAAAAA‑CCGCCAGTAAAACGGCGGTGAATGCTTTCATGGAAAGAATTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTTCACTGATAAAGAATCGTTGACACAGTAGCATCAATTTTTTCAATGAATGt                   >  2:1043680/1‑151 (MQ=255)
                                                                                                                                         tgttttttATGAGGGCAAAAAAAAA‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGTTTTCTCAATGAATGt                   <  1:1159723/147‑1 (MQ=255)
                                                                                                                                          gATTTTTATGACGGCGAAAAAAAA‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGTTTTCTCAATGAATGtt                  >  1:1068172/1‑150 (MQ=255)
                                                                                                                                           ttttttATGCCGGAAAAAAAAAA‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGTTTTCTCAATGAATGTTa                 <  1:6839/149‑1 (MQ=255)
                                                                                                                                           ttttttATGACGGCAAAAAAAAA‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGTTTTCTCAATGAATGTTa                 <  2:596864/149‑1 (MQ=255)
                                                                                                                                            tttttATGAGGGAAAAAAAAAA‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGTTTTCTCAATGAATGTTaa                <  1:573313/150‑1 (MQ=255)
                                                                                                                                             ttttATGACGGCGAAAAAAAA‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGTTTTCTCAATGAATGTTAAAc              >  2:428955/1‑151 (MQ=255)
                                                                                                                                             ttttATGACGGCGAAAAAAAA‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGTTTTCTCAATGAATGTTAAAc              >  1:727480/1‑151 (MQ=255)
                                                                                                                                             ttttATGACGGAAAAAAAAAA‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGTTTTCTCAATGAATGTTaaa               <  2:1349718/150‑1 (MQ=255)
                                                                                                                                              tttATGACGGCGAAAAAAAA‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGTTTTCTCAATGAATGTTAAACg             >  2:479765/1‑151 (MQ=255)
                                                                                                                                                tATGACGGCGAAAAAAAA‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGTTTTCTCAATGAATGTTAAACgg            >  1:340076/1‑150 (MQ=255)
                                                                                                                                                aaTGACGGAAAAAAAAAA‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGTTTTCTCAATGAATGTTAAACgg            <  1:1367548/149‑1 (MQ=255)
                                                                                                                                                        cGAAAAAAAA‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGTTTTCTCAATGAATGTTAAACGGAGCTTAAAc   >  1:223975/1‑151 (MQ=255)
                                                                                                                                                         gAAAAAAAA‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGTTTTCTCAATGAATGTTAAACGGAGCTTAAACt  >  1:1175794/1‑151 (MQ=255)
                                                                                                                                                         |                                                                                                                                                       
TTTTATGGCCGTACTGGGAATAATTTTATTTTCTGGTTTTCTGGCCGCGTATTTCAGCCACAAA‑TGGGATGACTAATGAACGGAGATAATCCCTCACCTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTATGACGGCGAAAAAAAA‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGTTTTCTCAATGAATGTTAAACGGAGCTTAAACT  >  NC_000913/1622666‑1622966

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑