Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 450,073 C→T W190* (TGG→TAG)  cyoB ← cytochrome o ubiquinol oxidase subunit I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913450,0730CT98.2% 439.6 / ‑2.1 112W190* (TGG→TAG) cyoBcytochrome o ubiquinol oxidase subunit I
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base T (55/55);  total (55/57)
Fisher's exact test for biased strand distribution p-value = 4.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 112 total aligned reads displayed.

ATCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCCAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGTTAACCAGAATCACACCAACAACGGTAAACCAGAAGCTTAAGTTGTTG  >  NC_000913/449926‑450219
                                                                                                                                                   |                                                                                                                                                  
atCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGt                                                                                                                                                 <  1:1468107/151‑1 (MQ=255)
  cAGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTa                                                                                                                                                >  1:245666/1‑150 (MQ=255)
   aGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAAt                                                                                                                                              <  1:898336/151‑1 (MQ=255)
   aGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAAt                                                                                                                                              <  1:1249352/151‑1 (MQ=255)
   aGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAAt                                                                                                                                              >  2:561446/1‑151 (MQ=255)
       cGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGac                                                                                                                                          <  2:1202719/151‑1 (MQ=255)
        gTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGac                                                                                                                                          >  2:37352/1‑150 (MQ=255)
        gTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGac                                                                                                                                          >  2:238006/1‑150 (MQ=255)
        gTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGac                                                                                                                                          >  2:54924/1‑150 (MQ=255)
           cgcgCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCg                                                                                                                                      <  1:1148978/151‑1 (MQ=255)
             cgcACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACTCCGAc                                                                                                                                    <  2:588665/151‑1 (MQ=255)
             cgcACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGAc                                                                                                                                    >  1:1449032/1‑151 (MQ=255)
              gcACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACAGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACt                                                                                                                                   >  1:183249/1‑151 (MQ=255)
                acaGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTcc                                                                                                                                 >  2:1259811/1‑151 (MQ=255)
                   gTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCgg                                                                                                                              <  1:460674/151‑1 (MQ=255)
                   gTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCgg                                                                                                                              <  1:794645/151‑1 (MQ=255)
                     gATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGAc                                                                                                                            >  1:1275025/1‑151 (MQ=255)
                     gATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGAc                                                                                                                            >  2:358287/1‑151 (MQ=255)
                        gCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTg                                                                                                                          <  2:301736/150‑1 (MQ=255)
                        gCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGt                                                                                                                         <  1:41009/151‑1 (MQ=255)
                             ggTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTActct                                                                                                                    <  2:292620/151‑1 (MQ=255)
                             ggTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTActct                                                                                                                    >  2:1075168/1‑151 (MQ=255)
                               tAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTAt                                                                                                                  >  2:172733/1‑151 (MQ=255)
                                aaaTACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTAtt                                                                                                                 >  1:1180720/1‑151 (MQ=255)
                                 aaTACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTc                                                                                                                >  2:175602/1‑151 (MQ=255)
                                       ggCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGAt                                                                                                           <  2:474438/150‑1 (MQ=255)
                                       ggCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATa                                                                                                          <  2:243185/151‑1 (MQ=255)
                                          aTCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCg                                                                                                       >  1:355171/1‑151 (MQ=255)
                                              tGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGt                                                                                                     <  1:374083/149‑1 (MQ=255)
                                               gAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTgg                                                                                                   >  1:849071/1‑150 (MQ=255)
                                               gAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTgg                                                                                                   >  2:1093320/1‑150 (MQ=255)
                                                 aCATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATa                                                                                                <  1:301733/151‑1 (MQ=255)
                                                  cATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAg                                                                                               <  1:1114904/151‑1 (MQ=255)
                                                   aTGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAgg                                                                                              >  1:577040/1‑151 (MQ=255)
                                                    tGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGgc                                                                                             >  1:1048531/1‑151 (MQ=255)
                                                       tCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGgcc                                                                                            >  1:248768/1‑149 (MQ=255)
                                                        cATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGgccagc                                                                                         >  1:30403/1‑151 (MQ=255)
                                                        cATGCCCGGGCCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGgccagc                                                                                         >  2:949275/1‑151 (MQ=255)
                                                         aTGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGgccagcc                                                                                        <  1:605038/151‑1 (MQ=255)
                                                         aTGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGgccagcc                                                                                        >  2:352351/1‑151 (MQ=255)
                                                         aTGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGgccagcc                                                                                        <  1:269753/151‑1 (MQ=255)
                                                           gCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGgccagcca                                                                                       <  2:955650/150‑1 (MQ=255)
                                                           gCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGgccagcc                                                                                        >  1:1368888/1‑149 (MQ=255)
                                                            cccGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCcagccagc                                                                                     <  2:508903/151‑1 (MQ=255)
                                                            cccGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCcagccagc                                                                                     >  1:871438/1‑151 (MQ=255)
                                                             ccGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCagccagcc                                                                                    >  2:88651/1‑151 (MQ=255)
                                                              cGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCg                                                                                   >  2:1431273/1‑151 (MQ=255)
                                                                gTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGt                                                                                 >  1:440315/1‑151 (MQ=255)
                                                                  gcgcgcATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTc                                                                                <  1:1336227/150‑1 (MQ=255)
                                                                       cATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTgcgc                                                                           >  1:552798/1‑150 (MQ=255)
                                                                        aTCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCa                                                                          >  1:1130933/1‑150 (MQ=255)
                                                                            tCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAAt                                                                       <  1:102329/149‑1 (MQ=255)
                                                                             cAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCg                                                                    >  2:1068198/1‑151 (MQ=255)
                                                                             cAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCg                                                                    >  2:1350260/1‑151 (MQ=255)
                                                                             cAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGCGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCg                                                                    >  2:894218/1‑151 (MQ=255)
                                                                              aGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGc                                                                   <  2:454954/151‑1 (MQ=255)
                                                                               gAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGcc                                                                  <  1:1302699/151‑1 (MQ=255)
                                                                                aaTGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGccc                                                                 <  1:1120042/151‑1 (MQ=255)
                                                                                 aTGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGccc                                                                 >  1:356086/1‑150 (MQ=255)
                                                                                 aTGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGccc                                                                 >  1:975294/1‑150 (MQ=255)
                                                                                   ggTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACg                                                              >  1:438657/1‑151 (MQ=255)
                                                                                   ggTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACg                                                              >  1:671114/1‑151 (MQ=255)
                                                                                    gTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGc                                                             >  2:1074302/1‑151 (MQ=255)
                                                                                        cGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCgag                                                         <  1:880999/151‑1 (MQ=255)
                                                                                        cGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCg                                                           >  2:310053/1‑149 (MQ=255)
                                                                                        cGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCg                                                           >  1:957893/1‑149 (MQ=255)
                                                                                          aagaagTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCgag                                                         >  2:305403/1‑149 (MQ=255)
                                                                                           agaagTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCgagaga                                                      <  1:175599/151‑1 (MQ=255)
                                                                                           agaagTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCgag                                                         >  2:345040/1‑148 (MQ=255)
                                                                                           agaagTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCAgagaga                                                      <  2:243670/151‑1 (MQ=255)
                                                                                            gaagTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGaa                                                     <  2:1449195/151‑1 (MQ=255)
                                                                                                 tgatcacgtcaaGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACCCCGACTCCCGCACTGTACTCCAGTCCCGATAGCGGTGGATAGGCCCGCCAGCCGATCTGCGCAAAGTCGCCCACGAAGAGAGAAACGtt                                                >  2:194940/11‑151 (MQ=255)
                                                                                                 tGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGtt                                                >  2:887758/1‑151 (MQ=255)
                                                                                                 tGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGtt                                                >  2:959690/1‑151 (MQ=255)
                                                                                                  gATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGtt                                                <  1:865152/150‑1 (MQ=255)
                                                                                                  gATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGtt                                                >  1:961547/1‑150 (MQ=255)
                                                                                                  gATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGtt                                                <  2:361941/150‑1 (MQ=255)
                                                                                                  gATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGtt                                                >  2:15932/1‑150 (MQ=255)
                                                                                                  gATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGTTa                                               <  2:1257119/151‑1 (MQ=255)
                                                                                                  gATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGTTa                                               <  2:628987/151‑1 (MQ=255)
                                                                                                        ggTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGTTAAcc                                            >  2:1338221/1‑148 (MQ=255)
                                                                                                        ggTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGTTAACCAGa                                         <  2:954856/151‑1 (MQ=255)
                                                                                                        ggTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGTTAACCAGa                                         <  2:78653/151‑1 (MQ=255)
                                                                                                           aaGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCCAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGTTAACCAGAAt                                       <  1:360950/150‑1 (MQ=255)
                                                                                                               ggcgtCCCTATACCGGATAGGGGGAGACTCCATATCCTGTAATCGACACCAACTCCCGTACTGTACACTATTCCCGATAGCTGTGGATAGGCCAGCCAGCCGCTCTGCGCAAATTCGCCCACGCCGAGAGAAACGTTAACCAGAATCacac                                  <  2:1044332/149‑1 (MQ=255)
                                                                                                                  gtACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGTTAACCAGAATCACACca                                >  2:139763/1‑150 (MQ=255)
                                                                                                                   tACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGTTAACCAGAATCACACcaac                              >  1:1161692/1‑151 (MQ=255)
                                                                                                                    aCCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGTTAACCAGAATCACACcaac                              <  2:109677/150‑1 (MQ=255)
                                                                                                                          aCCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGTTAACCAGAATCACACCAACAACGGTa                       <  1:395604/151‑1 (MQ=255)
                                                                                                                              gATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGTTAACCAGAATCACACCAACAACGGTAAAcc                   <  1:853200/151‑1 (MQ=255)
                                                                                                                              gATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGTTAACCAGAATCACACCAACAACGGTAAAcc                   <  1:697386/151‑1 (MQ=255)
                                                                                                                               aTAGCTGGAGACTCCATATCTAGTAATCGCCACCGACCCCCGGACTGTACTCTATTCCCGATACCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGTTAACCAGAAGCACACCAACAACGGTAAACCa                  <  2:697351/151‑1 (MQ=255)
                                                                                                                                    tGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGTTAACCAGAATCACACCAACAACGGTAAACCAGAAGc             >  2:1068561/1‑151 (MQ=255)
                                                                                                                                     ggAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGTTAACCAGAATCACACCAACAACGGTAAACCAGAAg              <  1:834103/149‑1 (MQ=255)
                                                                                                                                      gagaCTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGTTAACCAGAATCACACCAACAACGGTAAACCAGAAGCtt           <  2:1275024/151‑1 (MQ=255)
                                                                                                                                       agaCTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGTTAACCAGAATCACACCAACAACGGTAAACCAGAAGCTTa          <  1:238002/151‑1 (MQ=255)
                                                                                                                                        gaCTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGTTAACCAGAATCACACCAACAACGGTAAACCAGAAGCTTaa         <  1:857781/151‑1 (MQ=255)
                                                                                                                                            ccATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGTTAACCAGAATCACACCAACAACGGTAAACCAGAAGCTTAAgttg     >  1:68237/1‑151 (MQ=255)
                                                                                                                                            ccATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGTTAACCAGAATCACACCAACAACGGTAAACCAGAAGCTTAAgttg     >  1:1115948/1‑151 (MQ=255)
                                                                                                                                                atCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGTTAACCAGAATCACACCAACAACGGTAAACCAGAAGCTTAAGttgttg  <  1:1156050/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ATCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCCAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGTTAACCAGAATCACACCAACAACGGTAAACCAGAAGCTTAAGTTGTTG  >  NC_000913/449926‑450219

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: