Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,004,6250TG42.4% 220.4 / 20.1 125L227V (TTG→GTG) ycbFputative periplasmic pilin chaperone
Reads supporting (aligned to +/- strand):  ref base T (18/54);  new base G (53/0);  total (71/54)
Fisher's exact test for biased strand distribution p-value = 7.89e-20
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 126 total aligned reads displayed.

ACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGC‑AAAATC‑TGCCCTG‑AAAC‑AGGTTCGGAAAACG‑TTTGCGTTTTTTTTGCCGCAGGTC‑AATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACT  >  NC_000913/1004479‑1004774
                                                                                                                                                   |                                                                                                                                                           
aCATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTgtg                                                                                                                                                         >  1:521326/1‑149 (MQ=255)
aCATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGGTggg                                                                                                                                                         >  3:219169/1‑149 (MQ=255)
aCATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGGTGAg                                                                                                                                                         >  3:259912/1‑151 (MQ=255)
  aTTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTggggg                                                                                                                                                       >  2:783229/1‑147 (MQ=255)
  aTTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGGTGAGTg                                                                                                                                                       >  2:1102900/1‑151 (MQ=255)
    tAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGc                                                                                                                                                      <  4:937694/150‑1 (MQ=255)
     aaCCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCaa                                                                                                                                                    <  2:774781/151‑1 (MQ=255)
     aaCCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGGTgtgggga                                                                                                                                                     >  2:110025/1‑144 (MQ=255)
     aaCCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGGTgggggga                                                                                                                                                     >  2:24506/1‑144 (MQ=255)
     aaCCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGGTgggggga                                                                                                                                                     >  2:1063164/1‑144 (MQ=255)
      aCCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCaaa                                                                                                                                                   <  2:903630/151‑1 (MQ=255)
      aCCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCaaa                                                                                                                                                   <  4:1343603/151‑1 (MQ=255)
          gATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAaagaag                                                                                                                                               <  2:1111405/151‑1 (MQ=255)
          gATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAaagaa                                                                                                                                                <  2:237077/150‑1 (MQ=255)
           aTTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGGTgggggggaaaaaag                                                                                                                                              >  1:435264/1‑138 (MQ=255)
           aTTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGGTggggggcaaaaaag                                                                                                                                              >  3:392380/1‑138 (MQ=255)
           aTTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGGTTGGGGGAAAGAAgg                                                                                                                                              >  1:378491/1‑151 (MQ=255)
           aTTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGGTGAGGGGaaaaaaag                                                                                                                                              >  1:472203/1‑149 (MQ=255)
            ttCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGTGGGGAaagaaagg                                                                                                                                             >  2:285650/1‑148 (MQ=255)
            ttCAGGAGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGGTGGGGGGAAAGAAggg                                                                                                                                             >  4:777588/1‑150 (MQ=255)
             tCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGa                                                                                                                                             <  4:162954/150‑1 (MQ=255)
               aGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGC‑a                                                                                                                                         <  4:1202975/151‑1 (MQ=255)
                ggTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGTGGGCAAAAAAAGAGa‑aa                                                                                                                                        >  1:356892/1‑151 (MQ=255)
                ggTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGTGTGGTGCAAAGAAAGAGa‑aa                                                                                                                                        >  3:446214/1‑151 (MQ=255)
                   gAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGC‑aaaa                                                                                                                                      <  4:1374189/150‑1 (MQ=255)
                      tgtAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGC‑AAAATc                                                                                                                                    <  3:261188/149‑1 (MQ=255)
                       gtAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGGTGGGGGCGAAGAAAGAGG‑aaaaaatg                                                                                                                                  >  3:384043/1‑146 (MQ=255)
                        tAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGGTGGGTGGAAAAAAGGAGC‑aaaaaatccc                                                                                                                                >  4:1334208/1‑145 (MQ=255)
                            tGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGGTGGGTGGAAAGAAAGGGC‑caaaattggcctca                                                                                                                            >  1:128442/1‑141 (MQ=255)
                            tGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGGGGGGTGGAAAAAAAGAGG‑aaaaaatgacctcg                                                                                                                            >  3:435746/1‑141 (MQ=255)
                             ggTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGGGGGGAAAAAAAGAGG‑aaaaaattacctgaa                                                                                                                           >  3:493727/1‑140 (MQ=255)
                             ggTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGC‑AAAATC‑TGCCCTG‑aa                                                                                                                         <  2:824426/151‑1 (MQ=255)
                             ggTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGC‑AAAATC‑TGCCCTG‑aa                                                                                                                         <  4:1394992/151‑1 (MQ=255)
                             ggTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGGTGGGGGGGAAGAAAGAGG‑aaaaaattccccga                                                                                                                            >  2:340872/1‑140 (MQ=255)
                             ggTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGGTGGGGGGAAAAAAAGAGG‑AAAAAC‑TTccccca                                                                                                                           >  4:1203505/1‑147 (MQ=255)
                                aaaGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGC‑AAAATC‑TGCCCTG‑AAAc                                                                                                                       <  4:475000/150‑1 (MQ=255)
                                   gCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGGTGAGGGGGAAGAAAGGGC‑AAAAAA‑TGCCCCG‑AAAC‑aagg                                                                                                                  >  1:133858/1‑148 (MQ=255)
                                   gCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCAAGCGTCAGACAAGTTGGGGTCAGGCATTGGCCCCG‑GGCATGGTGGCCGGGCGGGCGAAAAATAATAACGCCGGGTTGGGGGGAAAAAAGAGGG‑AAAAAT‑TGCCCCG‑AAAA‑Aggg                                                                                                                  >  1:236948/1‑150 (MQ=255)
                                    cGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGC‑AAAATC‑TGCCCTG‑AAAC‑AGGtt                                                                                                                 <  4:515252/151‑1 (MQ=255)
                                     gTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGt                                                                                                                                                             <  1:38141/110‑1 (MQ=255)
                                     gTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGGTGAGGGCAAAGAAAGAGC‑AAAAAC‑TGCCCCG‑aaaaaaggtt                                                                                                                 >  1:113060/1‑144 (MQ=255)
                                      ttAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGGTTAGTGGAAAAAAAGGGG‑AAAAAA‑TTCCCTG‑GAAA‑AGGGTCg                                                                                                               >  2:955941/1‑151 (MQ=255)
                                       tAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGC‑AAAATC‑TGCCCTG‑AAAC‑AGGTTCg                                                                                                               <  2:834092/150‑1 (MQ=255)
                                       tAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGGTGTGTGCAAAGAAGGAGC‑AAAATC‑TGCCCTG‑AAAA‑AGGGTCgg                                                                                                              >  2:391972/1‑151 (MQ=255)
                                        aaGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGC‑AAAATC‑TGCCCTG‑AAAC‑AGGTTCGGa                                                                                                             <  4:74940/151‑1 (MQ=255)
                                        aaGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGGTTAGGGCAAAGAAAGAGC‑AAAAAC‑TGCCCCG‑AAAC‑AAGGTCggg                                                                                                             >  1:187871/1‑150 (MQ=255)
                                         aGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGC‑AAAATC‑TGCCCTG‑AAAC‑AGGTTCGGaa                                                                                                            <  2:822032/151‑1 (MQ=255)
                                          gTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGGTGGGGGGAAAAAAAGAGG‑AAAAAA‑TGACCCG‑AAAA‑AAGATCggga                                                                                                            >  4:1213236/1‑148 (MQ=255)
                                           tAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGC‑AAAATC‑TGCCCTG‑AAAC‑AGGTTCGGaa                                                                                                            <  3:14704/149‑1 (MQ=255)
                                            aaTGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGGTGAGTGCAAAAAAAGAGC‑AAAAAA‑TGCCCCGAAAAA‑AGGTTGGGaaa                                                                                                           >  2:946030/1‑150 (MQ=255)
                                             aTGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGGGGAAAAAAGGAGC‑AAAAAC‑TGCCCCC‑AAAA‑AAGGTCGGgaaaag                                                                                                        >  1:303137/1‑149 (MQ=255)
                                               gtgGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGGTGGGTGGAAAAAAGGGGG‑AAAAAA‑TTCCCCG‑AAAA‑AAGGTCGGGAAAAG‑tt                                                                                                     >  4:640728/1‑151 (MQ=255)
                                                tgGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGGGTGGGGCAAAGAAAGAGG‑AAAAAA‑TGCCCCG‑AAAA‑Aggggcggggaaagtgg                                                                                                     >  3:372304/1‑137 (MQ=255)
                                                 gggAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGGGTGGCAAAAAGGAGG‑AAAAAA‑TGCCCCGAAAAA‑AGGTTGGGAAAA‑G‑TTTg                                                                                                   >  2:592367/1‑151 (MQ=255)
                                                 gggAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGC‑AAAATC‑TGCCCTG‑AAAC‑AGGTTCGGAAAACG‑ttt                                                                                                    <  2:666645/150‑1 (MQ=255)
                                                  ggAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGGTGAGTGCAAAGAAAGAGC‑AAAAAA‑TGCCCCG‑AAAC‑AAGGTCGGGAAAAG‑GTTg                                                                                                   >  1:410639/1‑150 (MQ=255)
                                                   gAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGGTGGGTGGAAAAAAAGAGG‑AAAAAC‑TGTCCTG‑GAAA‑AAGGTCGGGAAACG‑GTTTCg                                                                                                 >  3:454122/1‑151 (MQ=255)
                                                             gccTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGGTGGGGGGAAAGAAGGAGG‑AAAAAA‑TGCCCCC‑AAAA‑AAGGTCGGAAAAAG‑GTTTTGtttttttttt                                                                                       >  4:76685/1‑149 (MQ=255)
                                                               cTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGGTGTGTGCGAAGAAGGAGG‑AAAAAA‑TGCCCTG‑CAAC‑AGGGTCGGAGAAAG‑TTTGCGtgttttttttcg                                                                                     >  3:391023/1‑147 (MQ=255)
                                                                  aaaGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGGTGAGGGGAAAGAAAGAGG‑AAAAACATCCCCGA‑AAAA‑AGGTTGGGAAAAGG‑TTTGGGttttttttttgc                                                                                     >  4:889802/1‑145 (MQ=255)
                                                                      ccAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGGTGGGTGGAAAGAAGGAGG‑AAAAAA‑TGCCCCG‑AAACAAGGTTGGGAAAA‑G‑GTTGCGGTTTTTTTTTCGCAgggt                                                                               >  2:876440/1‑148 (MQ=255)
                                                                      ccAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGGTGGGTGGAAAGAAGGAGCGAAATTT‑TGCCTTA‑AAAA‑AGGTTCGGAAAAGT‑TTTGGTTTTTTTTTGcgctgg                                                                                  >  2:50041/1‑143 (MQ=255)
                                                                        aGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGC‑AAAATC‑TGCCCTG‑AAAC‑AGGTTCGGAAAACG‑TTTGCGTTTTTTTTGCCGCAGGTC‑AAt                                                                           <  4:344964/151‑1 (MQ=255)
                                                                          cGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGC‑AAAATC‑TGCCCTG‑AAAC‑AGGTTCGGAAAACG‑TTTGCGTTTTTTTTGCCGCAGGTC‑AATTc                                                                         <  1:416323/151‑1 (MQ=255)
                                                                               gACAAGCTGGTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGGTGAGGGGAAAAAAGGAGG‑AAAATA‑TGTCCTG‑AAAC‑AGGGGCGGGAAACG‑GTTGTGTTTTTTTTTTCGCAGGGC‑AATTTCCttt                                                                    >  3:159432/1‑151 (MQ=255)
                                                                                  aaGCTGGTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGGTGAGTGCCAAGAAAGAGG‑AAAAAC‑TTCCCCG‑AAAA‑AAGGTCGGGAAACG‑GTTGTGTTTTTTTTTTCGCCGGGC‑AAATCCCcttttg                                                                 >  2:51732/1‑149 (MQ=255)
                                                                                      tGGTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGGTGGGGGGAAAAAAAGAGC‑AAAAAA‑TGCCCCG‑AAAA‑AAGGTCGGGAAAAG‑ATTTCGTTTTTTTTTTCGCCGGGC‑AAATTCCTTTTTGTTCg                                                             >  4:1356499/1‑151 (MQ=255)
                                                                                       ggTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGGGGGCAAAAAAAGAGC‑AAAAAA‑TGCCCCG‑AAAA‑AAGGTCGGGAAAAG‑TTTGGGTTTTTTTTTTCCCCGGGC‑AATTCCCTTTTTGTCCgg                                                            >  1:332682/1‑150 (MQ=255)
                                                                                       ggTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGC‑AAAATC‑TGCCCTG‑AAAC‑AGGTTCGGAAAACG‑TTTGCGTTTTTTTTGCCGCAGGTC‑AATTCCCTTTTGGTcc                                                              <  4:1165864/149‑1 (MQ=255)
                                                                                       ggTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGC‑AAAATC‑TGCCCTG‑AAAC‑AGGTTCGGAAAACG‑TTTGCGTTTTTTTTGCCGCAGGTC‑AATTCCCTTTTGGTcc                                                              <  2:934815/149‑1 (MQ=255)
                                                                                       ggTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGC‑AAAATC‑TGCCCTG‑AAAC‑AGGTTCGGAAAACG‑TTTGCGTTTTTTTTGCCGCAGGTC‑AATTCCCTTTTGGTcc                                                              <  2:423225/149‑1 (MQ=255)
                                                                                       ggTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGC‑AAAATC‑TGCCCTG‑AAAC‑AGGTTCGGAAAACG‑TTTGCGTTTTTTTTGCCGCAGGTC‑AATTCCCTTTTGGTCCg                                                             <  4:807245/150‑1 (MQ=255)
                                                                                         tgtCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGGTGAGGGGAAAAAAGGGGG‑AAAAAC‑TTCCCCG‑CAAC‑AAAATCGGGAAAAG‑ATTTCGTTTTTTTTTTTGTCGGTG‑AAATACCCTTTGGTCCgca                                                           >  3:272679/1‑148 (MQ=255)
                                                                                            cAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGC‑AAAATC‑TGCCCTG‑AAAC‑AGGTTCGGAAAACG‑TTTGCGTTTTTTTTGCCGCAGGTC‑AATTCCCTTTTGGTCCGAACTCg                                                       <  4:1376741/151‑1 (MQ=255)
                                                                                             aGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGGGCAAAGAAGGAGC‑AAAAAA‑TGCCCCG‑AAAC‑AGGGTCGGAAAAAG‑GTTGCGGTTTTTTTTTCGCAGGGCAAATTCCCTTTTGTTCC‑AACTCGc                                                      >  2:960053/1‑151 (MQ=255)
                                                                                                 aaTCGCTCCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGC‑AAAATC‑TGCCCTG‑AAAC‑AGGTTCGGAAAACG‑TTTGCGTTTTTTTTGCCGCAGGTC‑AATTCCCTTTTGGTCCGAACTCGCACat                                                  <  4:1375467/151‑1 (MQ=255)
                                                                                                 aaTCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGC‑AAAATC‑TGCCCTG‑AAAC‑AGGTTCGGAAAACG‑TTTGCGTTTTTTTTGCCGCAGGTC‑AATTCCCTTTTGGTCCGAACTCGCACat                                                  <  4:1387176/151‑1 (MQ=255)
                                                                                                  aTCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGC‑AAAATC‑TGCCCTG‑AAAC‑AGGTTCGGAAAACG‑TTTGCGTTTTTTTTGCCGCAGGTC‑AATTCCCTTTTGGTCCGAACTCGCACata                                                 <  2:1058005/151‑1 (MQ=255)
                                                                                                     gCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGGTGGGGGGAAAGAAGGAGG‑AAAAAA‑TGCCCCG‑AAACAAGGTCGGGAAAAGG‑TTTTGTTTTTTTTTTCCCCGGGCA‑AATTCCTTTTTGTTCC‑AACCCGCCCataaa                                               >  2:73658/1‑149 (MQ=255)
                                                                                                        ccGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGC‑AAAATC‑TGCCCTG‑AAAC‑AGGTTCGGAAAACG‑TTTGCGTTTTTTTTGCCGCAGGTC‑AATTCCCTTTTGGTCCGAACTCGCACATAATACGc                                           <  1:24815/151‑1 (MQ=255)
                                                                                                          gtgtCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGGTGGGGGGAAAGAAAGAGG‑AAAAAA‑TGCCCCG‑AAAC‑AAGGTCGGGAAACG‑GTTGCGTGTTTTTTTTCGCAGGGC‑AAATACCTTTTTTTCCGAACTCGCACATAATACGcc                                          >  3:566343/1‑150 (MQ=255)
                                                                                                          gtgtCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGGTGAGGGGAAAGAAGGAGC‑AAAAAA‑TGCCCCG‑AAAA‑AAGGTCGGGAAAAG‑GTTGGGGTTTTTTTTTCGCCGGGC‑AAATCCCTTTTTGTCCGAACTCGCACATAATACGcc                                          >  3:30122/1‑150 (MQ=255)
                                                                                                          gtgtCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGGTGAGGGCAAAGAAGGAGC‑AAAAAC‑TGCCCTG‑AAAA‑AAGGTCGGGAAACG‑TTTGCGTTTTTTTTTTCGCCGGTC‑AATTCCCTTTTTTTCCGAACTCGCACATAATAAGcc                                          >  3:479509/1‑150 (MQ=255)
                                                                                                            gtCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGGTGTGTGCAAAAAAGGAGC‑AAAAAA‑TGCCCTG‑AAAC‑AAGGTTGGAGAACGCTTTTGGTTTTTTTTTCGCCGGGGA‑AATTCCCTTTT‑GTCCGCACTCGCACATAAAACGccccc                                       >  2:267325/1‑151 (MQ=255)
                                                                                                              cAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGC‑AAAATC‑TGCCCTG‑AAAC‑AGGTTCGGAAAACG‑TTTGCGTTTTTTTTGCCGCAGGTC‑AATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCg                                      <  2:829920/150‑1 (MQ=255)
                                                                                                              cAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGC‑AAAATC‑TGCCCTG‑AAAC‑AGGTTCGGAAAACG‑TTTGCGTTTTTTTTGCCGCAGGTC‑AATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCg                                      <  4:110026/150‑1 (MQ=255)
                                                                                                               aT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGGTGGGTGGAAAAAAAGAGC‑AAAAAA‑TGCCCCGAAAAA‑AGGTTGGAAAAAGG‑TTTGGGTTTTTTTT‑TCGCAGGGC‑AAATTCCTTTTTGTTCGGAATCGCCCATAAAACcccccccgg                                    >  1:277065/1‑148 (MQ=255)
                                                                                                                       cTGGCGGGCGATAAATGATTACGGCGGGGGGGGTGCAAAAAAAGAGG‑CAAAAA‑TACCCTT‑AAAC‑AGGGTGGGGAAAAA‑TTTGTGGTTTTTTTTTCGCCGGGG‑AAATCCCTTTTTTTGCGAACACGCCCCAAAAACCCCCCCCGGTTGccc                              >  4:874653/1‑148 (MQ=255)
                                                                                                                         ggcgggcgATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGC‑AAAATC‑TGCCCTG‑AAAC‑AGGTTCGGAAAACG‑TTTGCGTTTTTTTTGCCGCAGGTC‑AATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACAcc                           <  2:859006/151‑1 (MQ=255)
                                                                                                                          gcgggcgATAAATGATTACGGCGGGTTGAGTGCAAAGAAAGAGC‑AAAAAC‑TGCCCCG‑AAAA‑AAGGTCGGGAAACA‑GTTTCGGTTTTTTTTTCGCCGGTCAAATTCCCTTTT‑GTCCGGAATCCCACATAATACGCCCCCCGTTTTCACAccc                          >  2:1059807/1‑150 (MQ=255)
                                                                                                                           cgggcgATAAATGATTACGGCGGGGTGAGGGGAAAAAAAGAGG‑AAAAAT‑TGGCCTG‑CAAA‑AGGATTGGAAAAAG‑TTTGCGTTTTTTTTTGCGTAGGGC‑AAATCCCTTTTTGTTCGCACACGGCCAAAAAAAGCCCCCCGGTTTCACAcctg                         >  2:1151844/1‑149 (MQ=255)
                                                                                                                                       gATTACGGCGGGTTGAGTGCAAAGAAGGAGC‑AAAATC‑TGCCCTG‑AAAC‑AGGTTCGGAAAACG‑TTTGCGTTTTTTTTGCCGCAGGTC‑AATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGgag              <  4:493875/150‑1 (MQ=255)
                                                                                                                                            cggcggGTTGAGTGCAAAGAAGGAGC‑AAAATC‑TGCCCTG‑AAAC‑AGGTTCGGAAAACG‑TTTGCGTTTTTTTTGCCGCAGGTC‑AATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCa        <  2:557305/151‑1 (MQ=255)
                                                                                                                                               cggGTTGAGTGCAAAGAAGGAGC‑AAAATC‑TGCCCTG‑AAAC‑AGGTTCGGAAAACG‑TTTGCGTTTTTTTTGCCGCAGGTC‑AATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGt     <  2:135587/151‑1 (MQ=255)
                                                                                                                                                gggTTGAGTGCAAAGAAGGAGC‑AAAATC‑TGCCCTG‑AAAC‑AGGTTCGGAAAACG‑TTTGCGTTTTTTTTGCCGCAGGTC‑AATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGta    <  2:376070/151‑1 (MQ=255)
                                                                                                                                                 ggTTGAGTGCAAAGAAGGAGC‑AAAATC‑TGCCCTG‑AAAC‑AGGTTCGGAAAACG‑TTTGCGTTTTTTTTGCCGCAGGTC‑AATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGtac   <  2:1401329/151‑1 (MQ=255)
                                                                                                                                                 ggTTGAGTGCAAAGAAGGAGC‑AAAATC‑TGCCCTG‑AAAC‑AGGTTCGGAAAACG‑TTTGCGTTTTTTTTGCCGCAGGTC‑AATGCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGtac   <  4:1422020/151‑1 (MQ=255)
                                                                                                                                                  gTTGAGTGCAAAGAAGGAGC‑AAAATC‑TGCCCTG‑AAAC‑AGGTTCGGAAAACG‑TTTGCGTTTTTTTTGCCGCAGGTC‑AATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGtact  <  2:151896/151‑1 (MQ=255)
                                                                                                                                                  gTTGAGTGCAAAGAAGGAGC‑AAAATC‑TGCCCTG‑AAAC‑AGGTTCGGAAAACG‑TTTGCGTTTTTTTTGCCGCAGGTC‑AATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGtac   <  3:435258/150‑1 (MQ=255)
                                                                                                                                                  gTTGAGTGCAAAGAAGGAGC‑AAAATC‑TGCCCTG‑AAAC‑AGGTTCGGAAAACG‑TTTGCGTTTTTTTTGCCGCAGGTC‑AATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGtac   <  3:136196/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                           
ACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCAT‑GTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGC‑AAAATC‑TGCCCTG‑AAAC‑AGGTTCGGAAAACG‑TTTGCGTTTTTTTTGCCGCAGGTC‑AATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACT  >  NC_000913/1004479‑1004774

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑