Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091329,4860CA44.9% 185.6 / 76.6 136intergenic (+291/‑165)dapB/carAdihydrodipicolinate reductase/carbamoyl phosphate synthetase small subunit, glutamine amidotransferase
Reads supporting (aligned to +/- strand):  ref base C (49/26);  new base A (0/61);  total (49/87)
Fisher's exact test for biased strand distribution p-value = 3.71e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 139 total aligned reads displayed.

TTTTGTTGTTTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGG‑TTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAAT  >  NC_000913/29337‑29633
                                                                                                                                                       |                                                                                                                                                   
   tgttgtTTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGG‑TTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACaaa                                                                                                                                                  <  2:576682/150‑1 (MQ=255)
     ttgttTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGG‑TTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAaat                                                                                                                                                <  1:12481/150‑1 (MQ=255)
        ttttAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGG‑TTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAaataat                                                                                                                                             >  2:1214378/1‑150 (MQ=255)
         tttAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGG‑TTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAatat                                                                                                                                           >  4:1396526/1‑151 (MQ=255)
          ttAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGG‑TTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAatat                                                                                                                                           >  2:1237436/1‑150 (MQ=255)
          ttAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGG‑TTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAatat                                                                                                                                           >  4:545542/1‑150 (MQ=255)
          ttAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGG‑TTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAatat                                                                                                                                           <  4:1447464/150‑1 (MQ=255)
          ttAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGG‑TTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAatat                                                                                                                                           <  4:1428086/150‑1 (MQ=255)
          ttAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGG‑TTAGATGATCTTTTTGCCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAatat                                                                                                                                           >  2:1449385/1‑150 (MQ=255)
           tAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGG‑TTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATaa                                                                                                                                         <  3:428700/151‑1 (MQ=255)
            aaTGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGG‑TTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATaaa                                                                                                                                        <  2:1455460/151‑1 (MQ=255)
            aaTGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGG‑TTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATaa                                                                                                                                         >  2:954514/1‑150 (MQ=255)
            aaTGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGG‑TTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATaa                                                                                                                                         >  4:741334/1‑150 (MQ=255)
             aTGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGG‑TTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATaaaa                                                                                                                                       <  2:331509/151‑1 (MQ=255)
                 aaaTTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGG‑TTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAt                                                                                                                                    <  2:1006471/150‑1 (MQ=255)
                 aaaTTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGG‑TTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAt                                                                                                                                    <  4:1079045/150‑1 (MQ=255)
                           cATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTCCC‑AGAACAGG‑TTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTaa                                                                                                                         >  1:203656/1‑151 (MQ=255)
                                ggTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGG‑TTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTg                                                                                                                     >  2:277151/1‑150 (MQ=255)
                                   ccttttttttttGCT‑TTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCTTTTTCA‑AAAACGGG‑TTAGATGATCTTTTTGCCGTTAAATGCCTGAAAAACATGCATGACCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACttt                                                                                                                <  2:716012/147‑1 (MQ=255)
                                     aCTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGG‑TTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACtttt                                                                                                               >  4:961334/1‑151 (MQ=255)
                                        tttttCTGCTTTTTTTTATTACATGTAATCTTCTGCCTGCCCAACCTTTTCCC‑AAAACGTT‑TAAGATTTTTTTTTTTTCACTTATTCCGTAAAAACCATGAATGAGCAAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACtttt                                                                                                               <  4:1159774/148‑1 (MQ=255)
                                        tttttCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGG‑TTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAgc                                                                                                            >  2:1331954/1‑151 (MQ=255)
                                         ttttCTGCTCTTTTTTTTTTCATGCATTTTTTTTGCTGCGCAAGCTTTTCCA‑AAAACAGT‑TTAGATGATTTTTTTGTCGTTTAATCCCTGAAAAACATGCATGACCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAgc                                                                                                            <  4:3324/150‑1 (MQ=255)
                                         ttttCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGG‑TTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAgcg                                                                                                           >  4:800200/1‑151 (MQ=255)
                                          tttCTGCTCTTTTTTATTTAATGCAATCTTCTTGCTGCGCAGGCGTTTTCC‑AGAACAGT‑TTATATGATTTTTTTGTCTTTTAATCCCTATAAAACATGCAGGAGCCAAAAAATAATATAAAAAACCCCGCCATTAAGTTGACTTTTAgcg                                                                                                           <  4:1389853/150‑1 (MQ=255)
                                            tCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGG‑TTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGccc                                                                                                        >  2:329903/1‑151 (MQ=255)
                                              tGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGG‑TTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCat                                                                                                      >  4:603400/1‑151 (MQ=255)
                                                    tttttATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGG‑TTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTcc                                                                                                >  4:708695/1‑151 (MQ=255)
                                                     ttttATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGG‑TTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTcc                                                                                                >  4:479379/1‑150 (MQ=255)
                                                     ttttATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCA‑AGAACAGT‑TTAGATGATTTTTTTGTCGTTTAATGCCTGAAAAACATGCATGAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTcc                                                                                                <  2:1098177/150‑1 (MQ=255)
                                                      tttATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGG‑TTAGATGCTCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACACAATAATATAAAAAATCCTGCCTTTAAGTTGACTTTTAGCGCCCATATCTcc                                                                                                >  4:16418/1‑149 (MQ=255)
                                                        tATTCCATCCATTCTTTTTGCTGCCCAAGTTTTTCCC‑AAAAAAGT‑TTAGATAATTTTTTTGTCGTTTACTGCTTAAAAAAAATGCATGACCCAAAAAATAAAATAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGa                                                                                             <  4:1190484/150‑1 (MQ=255)
                                                         ttttCATGCAATCTTCTTGCTGCGCAAGCTTTTTCC‑AGAACGGG‑TTAGATGATTTTTTTGTCGCTTAATGCCTGAAAAACATGCAGGAGCCACAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAAt                                                                                           <  1:49179/150‑1 (MQ=255)
                                                         ttttAATGCATTCTTTTTGCTGCGCAAGCTTTTTCA‑AGAACAGT‑TAAGATAATTTTTTTGTCGATTATTGCCTAAAAAACTTGCATGAGCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAAt                                                                                           <  3:408959/150‑1 (MQ=255)
                                                         aTTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGG‑TTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAAt                                                                                           >  4:139143/1‑151 (MQ=255)
                                                           ttCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGG‑TTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATgc                                                                                         >  2:119780/1‑151 (MQ=255)
                                                             cATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGG‑TTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATgccg                                                                                       >  3:444367/1‑151 (MQ=255)
                                                             cATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGG‑TTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATgccg                                                                                       >  4:1428073/1‑151 (MQ=255)
                                                                 aattttttttGCTGCGCAAGCTTTTCCA‑AGAACAGT‑TAAAATTATTTTTTTGTCGCTTAATCCCTAAAAAACATAAATGACCCAAAAAAAAATAAAAAAACCCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGt                                                                                   <  4:918289/143‑1 (MQ=255)
                                                                 aattcttctTGCGGCGCAGGCTTTTCCC‑AAAACAGG‑TAAGATAATTTTTTTGTCGTTTAATGCTTAAAAAACATGCATGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGt                                                                                   <  4:1222511/148‑1 (MQ=255)
                                                                  attcttttTGCTGCGCAAGC‑TTTTCC‑AAAACCGGTTTAGATATTTTTTTTGTCTTTTAATCCTTAAAAAACTTGATTGCCCCAAAAAAAAATAAAAAAACCCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGtt                                                                                  <  4:60269/149‑1 (MQ=255)
                                                                     cttcttGCTGCGCAAGCGTTTTCC‑AGAACAGG‑TTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGc                                                                               >  4:1111399/1‑151 (MQ=255)
                                                                      ttcttGCTGCGCAAGCGTTTTCC‑AGAACAGG‑CTAGATGATCTTTTTGTCGCTTAATGTCTGTAAAACATGCATGAGCTACAAAATAATATAAAAAATCCCGTCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGc                                                                               >  4:1197320/1‑150 (MQ=255)
                                                                       tcttGCTGCGCAAGCTTTTCCC‑AGAACAGG‑TTAGATGATTTTTTTGTCGTTTAATGCCTGAAAAACATGCATGAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGcc                                                                              <  2:936399/150‑1 (MQ=255)
                                                                         ttGCTGCGCAAGCGTTTTCA‑AAAACAGG‑TTAGATGATTTTTTTGTCGTTTAATGCCTATAAAACATGCAGGACCCAAAAAATAATATAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAg                                                                            <  4:107978/150‑1 (MQ=255)
                                                                          tgctgcGCAAGCGTTTTCC‑AGAACAGG‑TTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGa                                                                           >  4:861597/1‑150 (MQ=255)
                                                                           gctgcGCAAGCGTTTTCC‑AGAACAGG‑TTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGaa                                                                          >  2:995368/1‑150 (MQ=255)
                                                                              gcgcAAGCTTTTCCC‑AAAACAGT‑TTAAATGATTTTTTTGTCGCTTAAGGCCTGTAAAACATGCATGACCAAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAAtt                                                                       <  2:766409/150‑1 (MQ=255)
                                                                                 cAAGCGTTTTCC‑AGAACAGG‑TTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtcgtc                                                                   >  2:799974/1‑151 (MQ=255)
                                                                                 aaaGCTTTTTCA‑AAAACAGT‑TAAAATGATTTTTTTGTCGTTTAATGCCTGAAAAACATGCATGCGCCAAAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtcgtc                                                                   <  2:474886/150‑1 (MQ=255)
                                                                                   aGCGTTTTCC‑AGAACAGG‑TTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCgg                                                                 >  3:72942/1‑151 (MQ=255)
                                                                                    gCGTTTTCC‑AGAACAGT‑TTAGATAATTTTTTTGTCGCTTAATGCCTGAAAAACATGCATGACCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGt                                                                <  2:320888/151‑1 (MQ=255)
                                                                                      gTTTTCC‑AGAACAGG‑TTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTa                                                               >  2:528614/1‑150 (MQ=255)
                                                                                       ttttCC‑AGAAAAGG‑TTAGATGATTTTTTTGTCGCTTAATGCCTGAAAAACATGAATGACCCACAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTa                                                               <  2:525837/149‑1 (MQ=255)
                                                                                        tttCCAAGAACAGG‑TTAGATGATTTTTTTGTCTCTTAATGCCTTAAAAACATGCATGCGCAAAAAAAAAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTa                                                               <  4:642164/149‑1 (MQ=255)
                                                                                           cc‑AGAACAGG‑TTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGc                                                            >  2:1043225/1‑148 (MQ=255)
                                                                                              aGAACAGG‑TTAGATGATCTTTTTGTCGCTTAATGCCTGAAAAACATGCATGAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGAtt                                                       <  2:492897/151‑1 (MQ=255)
                                                                                                  cAGG‑TTAGATATTTTTTTTGTCGTTTAATGCCTGAAAAACATGCATGAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCa                                                   <  4:378740/151‑1 (MQ=255)
                                                                                                    gg‑TTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCAtt                                                 >  3:114103/1‑151 (MQ=255)
                                                                                                    gg‑TTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCAtt                                                 >  3:78530/1‑151 (MQ=255)
                                                                                                    gg‑TGAGATGATTTTTTTGTCGCTTAACGCAATAAAAACATGAATGGACCAAAAAATATTAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCAtt                                                 <  4:1333948/151‑1 (MQ=255)
                                                                                                     g‑TTAGATGATTTTTTTGTCGCTTAATGCTTGAAAAACATGCATGAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTg                                                <  2:775579/151‑1 (MQ=255)
                                                                                                     g‑TTAGATGATTTTTTTGTCGCTTAATGCCTGAAAAACATGCATGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTg                                                <  2:107045/151‑1 (MQ=255)
                                                                                                          gatgatCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGAttt                                            >  3:251902/1‑151 (MQ=255)
                                                                                                           atgatTTTTTTTTCGCTTAATGCTTGTAAAACATGCTTGAGCCAAAAAAAAATAAAAAAACTCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTa                                           <  4:383324/151‑1 (MQ=255)
                                                                                                             gatTTTTTTGCCGCTAAATGCCTGAAAAACATCCATGACCCAAAAAATAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTAc                                          <  2:440094/150‑1 (MQ=255)
                                                                                                              tttttttttTCGTTTAATGCCTGAAAAACATGCATGACCCACAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGt                                        <  1:98384/148‑1 (MQ=255)
                                                                                                              atttttttGTCGCTTATTCCCTGAAAAACATGAATGACCCAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGt                                        <  3:136260/148‑1 (MQ=255)
                                                                                                              atttttttGTCGCTTAATGCCTGAAAAACATGCATGAGCCACAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGt                                        <  1:251904/148‑1 (MQ=255)
                                                                                                              atCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGACCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGt                                        <  1:421030/151‑1 (MQ=255)
                                                                                                                ttttttGCCGTTTAATGCCTGAAAAACATGCATGAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtc                                       <  2:1386366/149‑1 (MQ=255)
                                                                                                                   tttGTCGTTTAATGCCTGTAAAACATGCATGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtcatc                                    <  2:1398344/150‑1 (MQ=255)
                                                                                                                   tttGTCGATAAATGCCTATAAAACAGGGAGGAGCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtcatc                                    <  2:36435/150‑1 (MQ=255)
                                                                                                                      gTCGTTTAATGCTTGTAAAACATGCATGACCAAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCAtt                                 <  4:633453/150‑1 (MQ=255)
                                                                                                                       tCGCTTAATGCTTGAAAAACATGCATGAGCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtg                                <  2:389372/150‑1 (MQ=255)
                                                                                                                        cctttaatcccgtaaaaaCATGCTTGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtgt                               <  4:243433/136‑1 (MQ=255)
                                                                                                                          cTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGa                             >  4:499313/1‑150 (MQ=255)
                                                                                                                          cTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTATTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGaa                            >  1:3105/1‑151 (MQ=255)
                                                                                                                           ttAATCCCTGAAAAACATGCATGACCCAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGaa                            <  4:257177/150‑1 (MQ=255)
                                                                                                                            tattGCCTGAAAAACATGCATGAGCCAAAAAATAATATAAAAATTCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGTAGATTTGCATTGATTTACGTCATCATTGTGaa                            <  2:1269147/146‑1 (MQ=255)
                                                                                                                            aaatcccttaaaaaCATGCATAGCCCAAAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAAtt                          <  2:60234/141‑1 (MQ=255)
                                                                                                                                gcctaaaaaaCATGCATGAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAata                      <  2:1454007/145‑1 (MQ=255)
                                                                                                                                 ccTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAatat                     >  3:492873/1‑151 (MQ=255)
                                                                                                                                  cTGTAAAACATGCATGACCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAata                      <  1:441865/149‑1 (MQ=255)
                                                                                                                                   tgaaaaaCATGCATGACCCAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGc                   <  3:204435/148‑1 (MQ=255)
                                                                                                                                    gaaaaaCATGCATGAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATg                    <  4:1360531/147‑1 (MQ=255)
                                                                                                                                      aaacCATGCATGGGCCACAAAATAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCaaa                <  4:657170/151‑1 (MQ=255)
                                                                                                                                      aaaaCATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCaa                 >  4:722717/1‑150 (MQ=255)
                                                                                                                                       aaaCATGCATGAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAAt               <  4:131033/151‑1 (MQ=255)
                                                                                                                                         aCATGCATGAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATaa             <  2:643788/151‑1 (MQ=255)
                                                                                                                                           ttgcatgGCCCAAAAAATAATATAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATaa             <  2:707575/148‑1 (MQ=255)
                                                                                                                                           atgcatgACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAag           <  1:463706/151‑1 (MQ=255)
                                                                                                                                           atgcatgACCCAAAAAATAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAag           <  3:193347/151‑1 (MQ=255)
                                                                                                                                            ggcggaggCCCCAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATaaa            <  4:1449388/142‑1 (MQ=255)
                                                                                                                                             gcatgAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtg         >  2:865349/1‑151 (MQ=255)
                                                                                                                                               atgAGCCACAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtgag       <  2:851136/151‑1 (MQ=255)
                                                                                                                                                 gacccAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtgagtg     <  2:74587/148‑1 (MQ=255)
                                                                                                                                                  acccAAAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAgtgagtga    <  2:1273842/149‑1 (MQ=255)
                                                                                                                                                   gCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAgtgagtga    >  2:249987/1‑150 (MQ=255)
                                                                                                                                                    ccACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAat  >  4:1005395/1‑151 (MQ=255)
                                                                                                                                                       |                                                                                                                                                   
TTTTGTTGTTTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGG‑TTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAAT  >  NC_000913/29337‑29633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑