Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091329,4960TA46.3% 135.5 / 85.1 134intergenic (+301/‑155)dapB/carAdihydrodipicolinate reductase/carbamoyl phosphate synthetase small subunit, glutamine amidotransferase
Reads supporting (aligned to +/- strand):  ref base T (51/21);  new base A (0/62);  total (51/83)
Fisher's exact test for biased strand distribution p-value = 2.44e-20
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 141 total aligned reads displayed.

TTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGG‑TTAGATGATCTTTTTGTCGCTTAATGC‑CTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTGG  >  NC_000913/29346‑29642
                                                                                                                                                         |                                                                                                                                                  
tttAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGG‑TTAGATGATCTTTTTGTCGCTTAATGC‑CTGTAAAACATGCATGAGCCACAAAATAatat                                                                                                                                                    >  2:1306208/1‑151 (MQ=255)
tttAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGG‑TTAGATGATCTTTTTGTCGCTTAATGC‑CTGTAAAACATGCATGAGCCACAAAATAatat                                                                                                                                                    >  4:1396526/1‑151 (MQ=255)
 ttAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGG‑TTAGATGATCTTTTTGTCGCTTAATGC‑CTGTAAAACATGCATGAGCCACAAAATAatat                                                                                                                                                    >  2:1237436/1‑150 (MQ=255)
 ttAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGG‑TTAGATGATCTTTTTGTCGCTTAATGC‑CTGTAAAACATGCATGAGCCACAAAATAatat                                                                                                                                                    <  4:1428086/150‑1 (MQ=255)
 ttAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGG‑TTAGATGATCTTTTTGCCGCTTAATGC‑CTGTAAAACATGCATGAGCCACAAAATAatat                                                                                                                                                    >  2:1449385/1‑150 (MQ=255)
  tAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGG‑TTAGATGATCTTTTTGTCGCTTAATGC‑CTGTAAAACATGCATGAGCCACAAAATAATATaa                                                                                                                                                  <  3:428700/151‑1 (MQ=255)
   aaTGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGG‑TTAGATGATCTTTTTGTCGCTTAATGC‑CTGTAAAACATGCATGAGCCACAAAATAATATaaa                                                                                                                                                 <  2:1455460/151‑1 (MQ=255)
   aaTGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGG‑TTAGATGATCTTTTTGTCGCTTAATGC‑CTGTAAAACATGCATGAGCCACAAAATAATATaa                                                                                                                                                  >  2:954514/1‑150 (MQ=255)
   aaTGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGG‑TTAGATGATCTTTTTGTCGCTTAATGC‑CTGTAAAACATGCATGAGCCACAAAATAATATaa                                                                                                                                                  >  4:741334/1‑150 (MQ=255)
    aTGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGG‑TTAGATGATCTTTTTGTCGCTTAATGC‑CTGTAAAACATGCATGAGCCACAAAATAATATaaaa                                                                                                                                                <  2:331509/151‑1 (MQ=255)
             ttGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGG‑TTAGATGATCTTTTTGTCGCTTAATGC‑CTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGcc                                                                                                                                       >  4:613683/1‑151 (MQ=255)
                  cATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTCCC‑AGAACAGG‑TTAGATGATCTTTTTGTCGCTTAATGC‑CTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTaa                                                                                                                                  >  1:203656/1‑151 (MQ=255)
                       ggTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGG‑TTAGATGATCTTTTTGTCGCTTAATGC‑CTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTg                                                                                                                              >  4:87231/1‑150 (MQ=255)
                       ggTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGG‑TTAGATGATCTTTTTGTCGCTTAATGC‑CTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTg                                                                                                                              >  2:277151/1‑150 (MQ=255)
                          ccttttttttttGCT‑TTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCTTTTTCA‑AAAACGGG‑TTAGATGATCTTTTTGCCGTTAAATGC‑CTGAAAAACATGCATGACCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACttt                                                                                                                         <  2:716012/147‑1 (MQ=255)
                            aCTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGG‑TTAGATGATCTTTTTGTCGCTTAATGC‑CTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACtttt                                                                                                                        >  4:961334/1‑151 (MQ=255)
                               tttttCTGCTTTTTTTTATTACATGTAATCTTCTGCCTGCCCAACCTTTTCCC‑AAAACGTT‑TAAGATTTTTTTTTTTTCACTTATTCC‑GTAAAAACCATGAATGAGCAAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACtttt                                                                                                                        <  4:1159774/148‑1 (MQ=255)
                               tttttCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGG‑TTAGATGATCTTTTTGTCGCTTAATGC‑CTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAgc                                                                                                                     >  2:1331954/1‑151 (MQ=255)
                                ttttCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGG‑TTAGATGATCTTTTTGTCGCTTAATGC‑CTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAgcg                                                                                                                    >  4:800200/1‑151 (MQ=255)
                                     tGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGG‑TTAGATGATCTTTTTGTCGCTTAATGC‑CTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCat                                                                                                               >  4:603400/1‑151 (MQ=255)
                                      gCTCTTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCTTTTTCC‑AGAACAGG‑TTAGATGATTTTTTTGTCGCTTAATGC‑CTGTAAAACATGCATGAGCCAAAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGccc                                                                                                                 <  2:1045095/148‑1 (MQ=255)
                                         cttttttATTTCATGAATTCTTCTTGTTGCGAAAGTTTTTCCC‑AGAAAAGT‑TTAGATGTTTTTTTTGGCGCTAAAGGC‑CTGAAAAACATGAATGGCCCAAAAAATAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCataa                                                                                                             <  4:1031955/147‑2 (MQ=255)
                                            ttttATTTCGTGCATTCTTTTTCCGGCGCAAGTTTTTCCG‑AGAGCGGC‑TAAGATGATCTTTTTCTCGC‑TAATGCAGTGTAAAACATCCATGAACCACAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTcc                                                                                                         <  2:399940/150‑1 (MQ=255)
                                            ttttATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGG‑TTAGATGATCTTTTTGTCGCTTAATGC‑CTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTcc                                                                                                         >  4:479379/1‑150 (MQ=255)
                                             tttATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGG‑TTAGATGCTCTTTTTGTCGCTTAATGC‑CTGTAAAACATGCATGAGCCACACAATAATATAAAAAATCCTGCCTTTAAGTTGACTTTTAGCGCCCATATCTcc                                                                                                         >  4:16418/1‑149 (MQ=255)
                                               tATTCCATCCATTCTTTTTGCTGCCCAAGTTTTTCCC‑AAAAAAGT‑TTAGATAATTTTTTTGTCGTTTACTGC‑TTAAAAAAAATGCATGACCCAAAAAATAAAATAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGa                                                                                                      <  4:1190484/150‑1 (MQ=255)
                                                ttttCATGCAATCTTCTTGCTGCGAAAGCTTTTCCC‑AGAACAGT‑TTAGATGATTTTTTTGTCGTTTAATGC‑CTGAAAAACATGCATGAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAAt                                                                                                    <  1:94917/150‑1 (MQ=255)
                                                aTTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGG‑TTAGATGATCTTTTTGTCGCTTAATGC‑CTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAAt                                                                                                    >  4:139143/1‑151 (MQ=255)
                                                aTTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGG‑TTAGATGATCTTTTTGTCGCTTAATGC‑CTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAAt                                                                                                    >  2:356843/1‑151 (MQ=255)
                                                    cATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGG‑TTAGATGATCTTTTTGTCGCTTAATGC‑CTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATgccg                                                                                                >  4:1428073/1‑151 (MQ=255)
                                                    cATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGG‑TTAGATGATCTTTTTGTCGCTTAATGC‑CTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATgccg                                                                                                >  3:444367/1‑151 (MQ=255)
                                                       gCAATCTTCTGGCTGCGCAAGCTTTTCCC‑AGAACAGG‑TTAGATGATTTTTTTGTCGCTTAATGC‑CTGAAAAACATGCATGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATgccgcc                                                                                              <  2:1181169/150‑1 (MQ=255)
                                                        aattttttttGCTGCGCAAGCTTTTCCA‑AGAACAGT‑TAAAATTATTTTTTTGTCGCTTAATCC‑CTAAAAAACATAAATGACCCAAAAAAAAATAAAAAAACCCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGt                                                                                            <  4:918289/143‑1 (MQ=255)
                                                        aattcttctTGCGGCGCAGGCTTTTCCC‑AAAACAGG‑TAAGATAATTTTTTTGTCGTTTAATGC‑TTAAAAAACATGCATGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGt                                                                                            <  4:1222511/148‑1 (MQ=255)
                                                         attcttttTGCTGCGCAAGC‑TTTTCC‑AAAACCGGTTTAGATATTTTTTTTGTCTTTTAATCC‑TTAAAAAACTTGATTGCCCCAAAAAAAAATAAAAAAACCCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGtt                                                                                           <  4:60269/149‑1 (MQ=255)
                                                            cttcttGCTGCGCAAGCGTTTTCC‑AGAACAGG‑TTAGATGATCTTTTTGTCGCTTAATGC‑CTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGc                                                                                        >  4:1111399/1‑151 (MQ=255)
                                                              tcttGCTGCGCAAGCGTTTTCC‑AGAACAGG‑TTAGATGATCTTTTTGTCGCTTAATGC‑CTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGcc                                                                                       >  2:799333/1‑150 (MQ=255)
                                                               tttGCGGCGCAAGGGTTTGCA‑AAAACAGG‑TTAGATGTTCTTTTTGTCCCTGACAGC‑ATGAAAAGCTGGCATGGACCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCa                                                                                      <  2:217675/149‑1 (MQ=255)
                                                                ttGCTGCGCAAGCGTTTTCA‑AAAACAGG‑TTAGATGATTTTTTTGTCGTTTAATGC‑CTATAAAACATGCAGGACCCAAAAAATAATATAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAg                                                                                     <  4:107978/150‑1 (MQ=255)
                                                                  gctgcGCAAGCGTTTTCC‑AGAACAGG‑TTAGATGATCTTTTTGTCGCTTAATGC‑CTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGaa                                                                                   >  2:995368/1‑150 (MQ=255)
                                                                     gcgcAAGCGTTTTCC‑AGAACAGG‑TTAGATGATTTTTTTGTCGCTTAATGC‑TTGAAAAACATGCAGGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtc                                                                               <  2:765223/151‑1 (MQ=255)
                                                                        cAAGCGTTTTCC‑AGAACAGG‑TTAGATGATCTTTTTGTCGCTTAATGC‑CTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtcgtc                                                                            >  2:799974/1‑151 (MQ=255)
                                                                        aaaGCGTTTCCC‑AGAACAGG‑TTAGATATTTTTTTTTTCCCTTAATCC‑CTGTAAAACATGCTTGACCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtcgtc                                                                            <  2:990527/150‑1 (MQ=255)
                                                                           gcttttcCC‑AGAACGGT‑TTAAATGATTTTTTTGTCGTTTAATGC‑TTGAAAAACATGCATGAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGt                                                                         <  2:293642/148‑1 (MQ=255)
                                                                           gCGTTTTCC‑AGAACAGT‑TTAGATAATTTTTTTGTCGCTTAATGC‑CTGAAAAACATGCATGACCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGt                                                                         <  2:320888/151‑1 (MQ=255)
                                                                              ttttCC‑AGAAAAGG‑TTAGATGATTTTTTTGTCGCTTAATGC‑CTGAAAAACATGAATGACCCACAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTa                                                                        <  2:525837/149‑1 (MQ=255)
                                                                               tttCCAAGAACAGG‑TTAGATGATTTTTTTGTCTCTTAATGC‑CTTAAAAACATGCATGCGCAAAAAAAAAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTa                                                                        <  4:642164/149‑1 (MQ=255)
                                                                                  cc‑AGAACAGG‑TTAGATGATCTTTTTGTCGCTTAATGC‑CTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGc                                                                     >  2:1043225/1‑148 (MQ=255)
                                                                                     aGAACAGG‑TTAGATGATCTTTTTGTCGCTTAATGC‑CTGAAAAACATGCATGAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGAtt                                                                <  2:492897/151‑1 (MQ=255)
                                                                                        aCAGG‑TTAGATGATTTTTTTGTCTTTTAAGCC‑CTAAAAAACATCCATGAGCCAAAAAATAAAAAAAAAAACCCCGCCATTAAGTTGACGTTTAGCGCCCATATCTCCAGAATGCCGAAGGTTGCCAGAAATTCGTCGGTAAGCAGATTTGc                                                             <  4:1369118/151‑1 (MQ=255)
                                                                                         cAGG‑TTAGATATTTTTTTTGTCGTTTAATGC‑CTGAAAAACATGCATGAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCa                                                            <  4:378740/151‑1 (MQ=255)
                                                                                          aGG‑TTAGATGATTTTTTTGTCGTTTAATGC‑CTGAAAAACATGCATGAGCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCAt                                                           <  4:1437486/151‑1 (MQ=255)
                                                                                           gg‑TTAGATGATCTTTTTGTCGCTTAATGC‑CTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCAtt                                                          >  3:78530/1‑151 (MQ=255)
                                                                                           gg‑TGAGATGATTTTTTTGTCGCTTAACGC‑AATAAAAACATGAATGGACCAAAAAATATTAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCAtt                                                          <  4:1333948/151‑1 (MQ=255)
                                                                                            g‑TTAGATGATTTTTTTGTCGCTTAATGC‑TTGAAAAACATGCATGAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTg                                                         <  2:775579/151‑1 (MQ=255)
                                                                                            g‑TTAGATGATTTTTTTGTCGCTTAATGC‑CTGAAAAACATGCATGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTg                                                         <  2:107045/151‑1 (MQ=255)
                                                                                                agattttttttttGTCGTTTATGGC‑CTTAAAACCATGCTTGACCCAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGAtt                                                      <  4:119194/143‑1 (MQ=255)
                                                                                                 gatgatCTTTTTGTCGCTTAATGC‑CTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGAttt                                                     >  3:251902/1‑151 (MQ=255)
                                                                                                    gttttttttGTCGTTTAATCC‑CTAAAAAAAATGCATGAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTAc                                                   <  4:3773/146‑1 (MQ=255)
                                                                                                    gatTTTTTTGCCGCTAAATGC‑CTGAAAAACATCCATGACCCAAAAAATAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTAc                                                   <  2:440094/150‑1 (MQ=255)
                                                                                                     tttttttttTCGTTTAATGC‑CTGAAAAACATGCATGACCCACAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGt                                                 <  1:98384/148‑1 (MQ=255)
                                                                                                     atttttttGTCGCTTATTCC‑CTGAAAAACATGAATGACCCAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGt                                                 <  3:136260/148‑1 (MQ=255)
                                                                                                     atttttttGTCGCTTAATGC‑CTGAAAAACATGCATGAGCCACAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGt                                                 <  1:251904/148‑1 (MQ=255)
                                                                                                     atCTTTTTGTCGCTTAATGC‑CTGTAAAACATGCATGACCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGt                                                 <  1:421030/151‑1 (MQ=255)
                                                                                                       ttttttGCCGTTTAATGC‑CTGAAAAACATGCATGAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtc                                                <  2:1386366/149‑1 (MQ=255)
                                                                                                          tttGTCGTTTAATGC‑CTGTAAAACATGCATGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtcatc                                             <  2:1398344/150‑1 (MQ=255)
                                                                                                              tCGCTTAATGC‑TTGAAAAACATGCATGAGCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtg                                         <  2:389372/150‑1 (MQ=255)
                                                                                                                cctttaatcccgtaaaaaCATGCTTGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtgt                                        <  4:243433/136‑1 (MQ=255)
                                                                                                                 cTTAATGC‑CTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGa                                      >  4:499313/1‑150 (MQ=255)
                                                                                                                 cTTAATGC‑CTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTATTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGaa                                     >  1:3105/1‑151 (MQ=255)
                                                                                                                  ttAATCC‑CTGAAAAACATGCATGACCCAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGaa                                     <  4:257177/150‑1 (MQ=255)
                                                                                                                   tattGC‑CTGAAAAACATGCATGAGCCAAAAAATAATATAAAAATTCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGTAGATTTGCATTGATTTACGTCATCATTGTGaa                                     <  2:1269147/146‑1 (MQ=255)
                                                                                                                   aaaTGC‑CTATAAAACAGGCATAGGCCACAAAAAAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAAt                                    <  2:892332/149‑1 (MQ=255)
                                                                                                                    aaatcccttaaaaaCATGCATGAGCCAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAAtt                                   <  3:249542/141‑1 (MQ=255)
                                                                                                                    aaatcccttaaaaaCATGCATAGCCCAAAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAAtt                                   <  2:60234/141‑1 (MQ=255)
                                                                                                                    aaTGC‑CTGTAAAACATGCATGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAAtt                                   <  2:545920/150‑1 (MQ=255)
                                                                                                                        gcctaaaaaaCATGCATGAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAata                               <  2:1454007/145‑1 (MQ=255)
                                                                                                                        c‑cTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAatat                              >  3:492873/1‑151 (MQ=255)
                                                                                                                        c‑cTGAAAAACATGCATGAGCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAata                               <  1:444373/150‑1 (MQ=255)
                                                                                                                           tgaaaaaCATGCATGACCCAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGc                            <  3:204435/148‑1 (MQ=255)
                                                                                                                            gaaaaaCATGCATGAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATg                             <  4:1360531/147‑1 (MQ=255)
                                                                                                                            gTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCa                           >  3:156092/1‑151 (MQ=255)
                                                                                                                              aaacCATGCATGGGCCACAAAATAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCaaa                         <  4:657170/151‑1 (MQ=255)
                                                                                                                              aaaaCATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCaa                          >  4:722717/1‑150 (MQ=255)
                                                                                                                               aaaCATGCATGAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAAt                        <  4:131033/151‑1 (MQ=255)
                                                                                                                                 aCATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATa                       >  2:125052/1‑150 (MQ=255)
                                                                                                                                 aCATGCATGAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATaa                      <  2:643788/151‑1 (MQ=255)
                                                                                                                                   ttgcatgGCCCAAAAAATAATATAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATaa                      <  2:707575/148‑1 (MQ=255)
                                                                                                                                   atgcatgACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAag                    <  1:463706/151‑1 (MQ=255)
                                                                                                                                    ggcggaggCCCCAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATaaa                     <  4:1449388/142‑1 (MQ=255)
                                                                                                                                     gcatgAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtg                  >  2:865349/1‑151 (MQ=255)
                                                                                                                                         gacccAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtgagtg              <  2:74587/148‑1 (MQ=255)
                                                                                                                                           gCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAgtgagtga             >  2:249987/1‑150 (MQ=255)
                                                                                                                                            ccACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAat           >  4:1005395/1‑151 (MQ=255)
                                                                                                                                                aaaaTAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATtc       >  2:1186312/1‑151 (MQ=255)
                                                                                                                                                aaaaTAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATtc       <  1:156094/151‑1 (MQ=255)
                                                                                                                                                  aataatAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATtctc     <  3:441730/151‑1 (MQ=255)
                                                                                                                                                   ataataTAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATtct      >  1:489528/1‑149 (MQ=255)
                                                                                                                                                   ataataTAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTctct    >  4:876770/1‑151 (MQ=255)
                                                                                                                                                    taataTAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTg   >  4:351731/1‑151 (MQ=255)
                                                                                                                                                         |                                                                                                                                                  
TTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGG‑TTAGATGATCTTTTTGTCGCTTAATGC‑CTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTGG  >  NC_000913/29346‑29642

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑