Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,622,8160GA43.3% 110.3 / 132.3 134intergenic (+75/+144)yneM/dgcZinner membrane‑associated protein/diguanylate cyclase, zinc‑sensing
Reads supporting (aligned to +/- strand):  ref base G (73/3);  new base A (0/58);  total (73/61)
Fisher's exact test for biased strand distribution p-value = 8.00e-35
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 137 total aligned reads displayed.

TTTTATGGCCGTACTGGGAATAATTTTATTTTCTGGTTTTCTGGCCGCGTATTTCAGCCACAAA‑TGGGATGACTAATGAACGGAG‑ATAATCCCTCACCTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTAT‑GACGGCGAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGTTTTCTCAATGAATGTTAAACGGAGCTTAAACT  >  NC_000913/1622666‑1622966
                                                                                                                                                           |                                                                                                                                                        
ttttATGGCCGTACTGGGAATAATTTTATTTTCTGGTTTTCTGGCCGCGTATTTCAGCCACAAA‑TGGGATGACTAATGAACGGAG‑ATAATCCCTCACCTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTAT‑GACGGCg                                                                                                                                                          >  1:359324/1‑151 (MQ=255)
  ttATGGCCGTACTGGGAATAATTTTATTTTCTGGTTTTCTGGCCGCGTATTTCAGCCACAAA‑TGGGATGACTAATGAACGGAG‑ATAATCCCTCACCTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTAT‑GACGGCGa                                                                                                                                                         >  2:774049/1‑150 (MQ=255)
  ttATGGCCGTACTGGGAATAATTTTATTTTCTGGTTTTCTGGCCGCGTATTTCAGCCACAAA‑TGGGATGACTAATGAACGGAG‑ATAATCCCTCACCTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTAT‑GACGGCGa                                                                                                                                                         <  2:1232249/150‑1 (MQ=255)
    aTGGCCGTACTGGGAATAATTTTATTTTCTGGTTTTCTGGCCGCGTATTTCAGCCACAAA‑TGGGATGACTAATGAACGGAG‑ATAATCCCTCACCTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTAT‑GACGGCGaaa                                                                                                                                                       <  2:730224/150‑1 (MQ=255)
    aTGGCCGTACTGGGAATAATTTTATTTTCTGGTTTTCTGGCCGCGTATTTCAGCCACAAA‑TGGGATGACTAATGAACGGAG‑ATAATCCCTCACCTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTAT‑GACGGCGaaa                                                                                                                                                       >  2:353963/1‑150 (MQ=255)
      ggCCGTACTGGGAATAATTTTATTTTCTGGTTTTCTGGCCGCGTATTTCAGCCACAAA‑TGGGATGACTAATGAACGGAG‑ATAATCCCTCACCTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTAT‑GACGGCGaaaaa                                                                                                                                                     >  4:6839/1‑150 (MQ=255)
      ggCCGTACTGGGAATAATTTTATTTTCTGGTTTTCTGGCCGCGTATTTCAGCCACAAA‑TGGGATGACTAATGAACGGAG‑ATAATCCCTCACCTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTAT‑GACGGCGaaaaa                                                                                                                                                     >  2:68771/1‑150 (MQ=255)
       gCCGTACTGGGAATAATTTTATTTTCTGGTTTTCTGGCCGCGTATTTCAGCCACAAA‑TGGGATGACTAATGAACGGAG‑ATAATCCCTCACCTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTAT‑GACGGCGaaaaaaa                                                                                                                                                   >  1:343847/1‑151 (MQ=255)
          gTACTGGGAATAATTTTATTTTCTGGTTTTCTGGCCGCGTATTTCAGCCACAAA‑TGGGATGACTAATGAACGGAG‑ATAATCCCTCACCTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTAT‑GACGGCGAAAAAAA‑A‑cc                                                                                                                                              >  4:697405/1‑151 (MQ=255)
          gTACTGGGAATAATTTTATTTTCTGGTTTTCTGGCCGCGTATTTCAGCCACAAA‑TGGGATGACTAATGAACGGAG‑ATAATCCCTCACCTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTAT‑GACGGCGAAAAAAA‑A‑cc                                                                                                                                              >  4:573311/1‑151 (MQ=255)
          gTACTGGGAATAATTTTATTTTCTGGTTTTCTGGCCGCGTATTTCAGCCACAAA‑TGGGATGACTAATGAACGGAG‑ATAATCCCTCACCTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTAT‑GACGGCGAAAAAAA‑A‑cc                                                                                                                                              >  2:981903/1‑151 (MQ=255)
               gggAATAATTTTATTTTCTGGTTTTCTGGCCGCGTATTTCAGCCACAAA‑TGGGATGACTAATGAACGGAG‑ATAATCCCTCACCTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTAT‑GACGGCGAAAAAAA‑A‑CCGCCAg                                                                                                                                         >  1:402083/1‑151 (MQ=255)
                   ataatTTTATTTTCTGGTTTTCGGGCCGGGTATTTCAGCCACAAA‑TGGGATGACTAATGAACGGAG‑ATAATCCCTCACCTAACCGGACCC‑TTGTTACAGTTGTGTACAAGGGGAA‑TGATTTTTAT‑GACGGCGAAAAAAA‑A‑CCGCCAGTaa                                                                                                                                      >  4:337079/1‑150 (MQ=255)
                           attttCTGGTTTTCTGGCCGCGTATTTCAGCCACAAA‑TGGGATGACTAATGAACGGAG‑ATAATCCCTCACCTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTAT‑GACGGCGAAAAAAA‑A‑CCGCCAGTAAACCGGCGGt                                                                                                                             >  2:997579/1‑151 (MQ=255)
                           attttCTGGTTTTCTGGCCGCGTATTTCAGCCACAAA‑TGGGATGACTAATGAACGGAG‑ATAATCCCTCACCTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTAT‑GACGGCGAAAAAAA‑A‑CCGCCAGTAAACCGGCGGt                                                                                                                             >  2:170838/1‑151 (MQ=255)
                             tttctggttttctggCCGCGTATTTCAGCCACAAA‑TGGGATGACTAATGAACGGAG‑ATAATCCCTCACCTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTAT‑GACGGCGAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTg                                                                                                                            >  4:1148652/1‑150 (MQ=255)
                              ttctgttttttgggCCGTTTATTTCAGCCAAAAG‑GGGGTTGACAAATGAAGGGAG‑ATAACCCCCCCCCAACCCGCCCCT‑TTTTTACGGTTGTGTAAAGGGGGCT‑TGTTTTTTAG‑GCCGGCAAAAAAAA‑C‑CCGCCAGTAAACCGGCGGTGa                                                                                                                           <  2:842255/150‑1 (MQ=255)
                               tctggttttctggCCGCGTATTTCAGCCACAAA‑TGGGATGACTAATGAACGGAG‑ATAATCCCTCACCTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTAT‑GACGGCGAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAAt                                                                                                                         >  2:964486/1‑151 (MQ=255)
                               tctggttttctggCCGCGTATTTCAGCCACAAA‑TGGGATGACTAATGAACGGAG‑ATAATCCCTCACCTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTAT‑GACGGCGAAAAAAA‑A‑CCGCCAGTAAACCGGCGGGGAAt                                                                                                                         >  2:910887/1‑151 (MQ=255)
                                ttgtttttcgggCGGCTTTTTTCAGCCAAAAA‑GGGGATGACTAATGAGGGGAG‑ATAACCCCCCCCCTACCCGCCCCT‑TTGTTACGGTGGTGTACAGGGGGCC‑TTTTTTTTAG‑GACGGCAAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAAt                                                                                                                         <  2:98463/146‑1 (MQ=255)
                                      ttctggCCGCTTTTTCCAGCCAAAAA‑GGGAATGACAAAGGAAGGGAA‑AAAACCCCTCCCCTAACCGCCCCT‑TTGTTACGGTGGTGAAAAGGGGGCT‑TGTTTTTTAG‑GAGGGAAAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAATGCTTGc                                                                                                                   <  4:550056/150‑1 (MQ=255)
                                      ttcgggCCGCTTTTTCCACCCAAAAG‑GGGGATGACTAAGGAGGGGAA‑ATAACCCCCCCCCAACCCGCCCCT‑TTGTAACGGTTGTGTACAGGGGGCT‑TATTTTTTAG‑GAGGGGAAAAAAAA‑C‑CCGCCAGTAAACCGGCGGTGAATGCTTGc                                                                                                                   <  4:120253/150‑1 (MQ=255)
                                      ttcggcccGCTTATTCCACCCAAAAA‑GGGGATGATTAATGAGCGGAG‑ATAACCCCCCCCCTACCCGCCCCT‑TTGTTCCGGTTGTGAAAAGGGGGCT‑TGTTTTTTAT‑GACGGAAAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAATGCTTGc                                                                                                                   <  4:1426373/144‑1 (MQ=255)
                                          gcccGCGTATTCCAGCCACAAG‑TGGGAGGAAAAAGGACGGAAA‑ACAGCCCCTCTCCAATCCCCCCTC‑TTGTCACTGTTGTTAAAAAGGGTCC‑TGTTTTTGAT‑GGCGGAAAAAAAAA‑C‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATgg                                                                                                               <  4:216147/148‑1 (MQ=255)
                                           ggcgcgTATTCCAGCAAGAAG‑GGGGTTAACTAAAGAA‑GGAGAATAAGCCCTCCCCTACCCGCCCCC‑TGGTTACTTTTGTTTA‑AAGGGGCC‑CTAATTTTGA‑GGCGGAAAAAAAAATG‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGAt                                                                                                             <  2:1437036/149‑1 (MQ=255)
                                           cccGCGTTTTACACCCAAAAA‑GGGGATAACTAAGAAACGGAG‑AAACCCCCTCACCTACCCGGCCCT‑TTGTTACAGTTGTGAAAAGGGGGCT‑TGATTTTTGG‑GCCGGAAAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGAt                                                                                                             <  3:64256/150‑1 (MQ=255)
                                            ccGCTTATTT‑AGCCAAAAAGTGGGTTAATAAAAGAGCGGAG‑AAACCCCCTCCCCTACCCGCCCCT‑TTTTTAGATTGGTAAACAGGGGTCG‑TTTTTTTTTT‑CACGGAAAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATa                                                                                                            <  4:854493/151‑1 (MQ=255)
                                                ttATTCCAGCCAAAAA‑TGGGATGACAAATGAAGGGAG‑AAAACCCCTCCCCTACCCGCCCCC‑TTTTTACAGTTGTGTACAGGGGGCT‑TGTTTTTTAG‑GACGGAAAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGAtt                                                                                                        <  1:210178/150‑1 (MQ=255)
                                                gTATTTCAGCCACAAA‑TGGGATGACTAATGAACGGAG‑ATAATCCCTCACCTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTAT‑GACGGCGAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGAtt                                                                                                        >  2:1255087/1‑151 (MQ=255)
                                                  tttccAGCCAAAAA‑GGGGATGAATAATGAAGGGAA‑ATAACCCCCCCCCTACCCGGCCCT‑TTGTTACGTTTGTGTACAAGGGGCC‑TGTTTTTTAT‑GCCGGAAAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGAttt                                                                                                       <  2:1226022/146‑1 (MQ=255)
                                                   tttCACCCAAAAA‑GGGGATGACTAATGAACGGAA‑ATAACCCCCCCCCAACCCGCCCCC‑TTGTTACATTTGTGTAAAGGGGGCT‑TTTTTTTTAG‑GAGGGAAAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTtg                                                                                                      <  4:1400193/150‑1 (MQ=255)
                                                    ttcagatccaAA‑TGGGATGACTAATGAACGGAG‑ATAACCCCTCACCTAACCGGCCGC‑TTGTTATCGTTGTGTACAAGGGGCC‑TGATTTTTAT‑GACGCCGAAAAAAG‑A‑CCGCCAGTAAACCGGCGGAGAATGCTTGCATGGATAGATTggtg                                                                                                    >  4:177752/9‑151 (MQ=255)
                                                     tcaacaacaAA‑TGGGATGACTAATGAGGGGAA‑ATATCCCCCCCCCTACCCGGCCCT‑TTGTTACGGTTGTTTACAGGGGCCT‑TTTTTTTTAT‑GAGGGAAAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTgtgt                                                                                                   <  3:86704/145‑1 (MQ=255)
                                                            caAA‑TGGGATGACTAATGAACGGAG‑ATAATCCCTCACCTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTAT‑GACGGCGAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCtt                                                                                            >  2:942717/1‑151 (MQ=255)
                                                            caAA‑TGGGATGACTAATGAACGGAG‑ATAATCCCTCACCTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTAT‑GACGGCGAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCtt                                                                                            >  1:316396/1‑151 (MQ=255)
                                                              aaagggggttaatAAAGAACGGAA‑AAAACCCCCCCCCCAACCCGCCCCTTTTTTACTTTTTTGTAAAGGGGGCT‑TTTTTTTTAG‑GCGGGAAAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCtt                                                                                            <  4:1115962/139‑1 (MQ=255)
                                                              aa‑TGGGATGACTAATGAACGGAG‑ATAATCCCTCACCTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTAT‑GACGGCGAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGTAAGATTTGTGTTTTGCtttt                                                                                          >  4:269821/1‑151 (MQ=255)
                                                               a‑TGGGATGACTAATGAACGGAG‑ATAATCCCTCACCTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTAT‑GACGGCGAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGGTTTGCtttt                                                                                          >  4:216585/1‑150 (MQ=255)
                                                                    gATGACTAATGAACGGAG‑ATAATCCCTCACCTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTAT‑GACGGCGAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCt                                                                                     >  2:474828/1‑151 (MQ=255)
                                                                         aaaaTGAAGGGAA‑AAAACCCCCCCCCTACCCGCCCCT‑TTTTTACGTTTGTGTAAAGGGGGCT‑TTTTTTTTAT‑GAGGGGAAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAg                                                                                <  4:1179985/149‑1 (MQ=255)
                                                                            aTGAACGGAG‑ATAATCCCTCACCTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTAT‑GACGGCGAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGc                                                                              >  2:714780/1‑150 (MQ=255)
                                                                              gAACGGAA‑ATACCCCCTCCCCAACCCGCCCCC‑TTGTTACGTTTGTGTACAAGGGGCC‑TTTTTTTTAG‑GGCGGAAAAAAAAA‑G‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCAtt                                                                           <  4:766095/151‑1 (MQ=255)
                                                                               aaCGGAG‑ATAATCCCTCACCTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTAT‑GACGGCGAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCAttt                                                                          >  1:257964/1‑151 (MQ=255)
                                                                                cgggAG‑ATAACCCCTCCCCTACCCGGCCCT‑TTTTTACAGTTGTGTAAAAGGGGCT‑TGTTTTTTAT‑GACGGCAAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCAtttt                                                                         <  2:269256/149‑1 (MQ=255)
                                                                                 cGGAG‑ATAATCCCTCACCTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTAT‑GACGGCGAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGGGTTTTGCTTTTACGCTAACAGGCATTTTc                                                                        >  2:1032955/1‑151 (MQ=255)
                                                                                   gag‑aTAATCCCTCACCTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTAT‑GACGGCGAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTc                                                                        >  3:450009/1‑149 (MQ=255)
                                                                                    gaaaTAACCCCCCCCCAACCCGCCCCT‑TTTTTACGGTTGTGTA‑AAGGGGGC‑TTGTTTTTATGGCCGGAAAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTcc                                                                       <  3:536328/147‑1 (MQ=255)
                                                                                        aactCCCCCCCCTACCCGCCCCT‑TTTTTACAGTTTTGAAAAAGGGGCT‑TGTTTTTTAT‑GAGGGAAAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCAc                                                                  <  3:327623/148‑1 (MQ=255)
                                                                                         aaTCCCTCACCTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTAT‑GACGGCGAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACt                                                                 >  4:1124353/1‑151 (MQ=255)
                                                                                           tCCCTCACCTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTAT‑GACGGCGAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGCATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGAATTTTCCTGCACTg                                                                >  4:1346397/1‑150 (MQ=255)
                                                                                           tCCCTCACCTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTAT‑GACGGCGAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGa                                                               >  3:178821/1‑151 (MQ=255)
                                                                                            ccccccccaacccGCCCCT‑TTTTTACATTTGTTAACAGGGGGCT‑TTTTTTTTAG‑GCGGGAAAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGAt                                                              <  4:980710/140‑1 (MQ=255)
                                                                                            cccTCACCTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTAT‑GACGGCGAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGAt                                                              >  2:1234594/1‑151 (MQ=255)
                                                                                            cccTCACCTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTAT‑GACGGCGAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGAt                                                              >  2:7973/1‑151 (MQ=255)
                                                                                             ccacACCAACCCGGCCTG‑TGGTTAAATTTGTTTACAGGGGCCT‑TTTTTTTTTT‑GACGGAAAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATa                                                             <  4:581070/148‑1 (MQ=255)
                                                                                             ccTCACCTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTAT‑GACGGCGAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGAt                                                              >  4:1436817/1‑150 (MQ=255)
                                                                                               cccccTACCCGCCCCT‑TTTTTAATTTTGTGTAAAAGGGCCT‑TGTTTTTAAT‑GGCGCAAAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAAc                                                           <  2:426347/148‑1 (MQ=255)
                                                                                                ccccTACCCGCCCCT‑TTTTTACATTTGTGTACAGGGGGCT‑TGTTTTTTAG‑GACGGCAAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACg                                                          <  2:487063/149‑1 (MQ=255)
                                                                                                ccccTACCCGCCCCT‑TTGTTCCATTTTTGAAAAAGGGCCC‑TG‑TTTTGAC‑GGGGGGAAAAAAAA‑AGCCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAAc                                                           <  2:204665/148‑1 (MQ=255)
                                                                                                 aCCTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTAT‑GACGGCGAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACg                                                          >  4:1364682/1‑150 (MQ=255)
                                                                                                  ccTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTAT‑GACGGCGAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGa                                                         >  4:717273/1‑150 (MQ=255)
                                                                                                     acccGCCCCC‑TTTTTACATTTGTG‑AAAAGGGGCCTTGTTTTTTAG‑GCCGGAAAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCg                                                     <  2:284655/149‑1 (MQ=255)
                                                                                                        cgcccct‑TTTTTACGGTTGTGTAAAGGGGGCT‑TGTTTTTTAG‑GCCGGAAAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTg                                                  <  2:697408/148‑1 (MQ=255)
                                                                                                        cGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTAT‑GACGGCGAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTg                                                  >  2:1205148/1‑151 (MQ=255)
                                                                                                          cccct‑TTTTTACAGTTGTGTACAGGGGGCT‑TGTTTTTTAG‑GACGGAAAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGac                                                <  2:274692/150‑1 (MQ=255)
                                                                                                          cccct‑TTGTTACAGTTGTGAAAAGGGGGCT‑TTTTTTTTAT‑GACGGAAAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGac                                                <  2:406074/150‑1 (MQ=255)
                                                                                                           cccc‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTAT‑GACGGCGAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGac                                                >  4:678067/1‑150 (MQ=255)
                                                                                                             cctttGTTAGGGTGGTGTACAGGGGGCT‑TTTTTTTTAT‑GAGGGAAAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGacac                                              <  4:769344/148‑1 (MQ=255)
                                                                                                                ttGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTAT‑GACGGCGAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGt                                           >  3:414531/1‑151 (MQ=255)
                                                                                                                 tGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTAT‑GACGGCGAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTa                                          >  3:286866/1‑151 (MQ=255)
                                                                                                                   ttACAGTTGTGTACAAGGGGCC‑TGATTTTTAT‑GACGGCGAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGc                                        >  4:360050/1‑151 (MQ=255)
                                                                                                                     acatttgttaaaaggggcCC‑TGTTTTTTAG‑GCCGGAAAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCa                                       <  2:1191047/136‑1 (MQ=255)
                                                                                                                     aCAGTTGTGTACAAGGGGCC‑TGATTTTTAT‑GACGGCGAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCAt                                      >  2:547/1‑151 (MQ=255)
                                                                                                                      gaGTTGTATAAAGGGGGCC‑TGATTTTTAT‑GACGGAAAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATc                                     <  2:1032329/150‑1 (MQ=255)
                                                                                                                        gTTGTGTACAAGGGGCC‑TGATTTTTAT‑GACGGCGAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAg                                   >  3:540267/1‑151 (MQ=255)
                                                                                                                         ttGTGTAAAAGGGCCC‑GTTTTTTTAT‑GAGGGAAAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCAt                                      <  3:377062/147‑1 (MQ=255)
                                                                                                                           gtgtACAAGGGGCC‑TGATTTTTAT‑GACGGCGAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGttt                                >  3:208472/1‑151 (MQ=255)
                                                                                                                           gtgtAAAGGGGGCT‑TTTTTTTTAG‑GGCGGAAAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGttt                                <  2:359620/151‑1 (MQ=255)
                                                                                                                           gtgtAAAAGGGGCT‑TTTTTTTTAG‑GAGGGCAAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGtt                                 <  4:528552/150‑1 (MQ=255)
                                                                                                                            tgtACAAGGGGCC‑TGATTTTTAT‑GACGGCGAAAAAAA‑A‑CCGCCAGTAAACCGGCGGAGAATGCTTGCATGGATAGGTTTGTGGTTTGCTTTTACGCTAACAGGCATTTTCCCGCACTGATAAAGAATCGTTGTCACAGTAGCATCAGtttt                               >  2:88920/1‑151 (MQ=255)
                                                                                                                               aCAAGGGGCC‑TGATTTTTAT‑GACGGCGAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGTTTtctc                            >  2:631093/1‑151 (MQ=255)
                                                                                                                                  gggcgCC‑TTATTTTTAT‑GCGGACAAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGTTTTCTCaat                         <  2:1149326/147‑1 (MQ=255)
                                                                                                                                   ggggCC‑TTTTTTTTAG‑GACGGAAAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGTTTTCTCaatg                        <  2:406651/151‑1 (MQ=255)
                                                                                                                                    gggCT‑TTATTTTTAT‑GCGGGAAAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGTTTTNNCaatga                       <  4:1259002/151‑1 (MQ=255)
                                                                                                                                    gggCC‑TGATTTTTAT‑GACGGCGAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGTTTTCTCaatga                       >  1:299376/1‑151 (MQ=255)
                                                                                                                                     gggctttttttttAT‑GCGGGCAAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGTTTTCTCaatga                       <  4:484534/143‑1 (MQ=255)
                                                                                                                                        c‑TGATTTTTAT‑GACGGCGAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGTTTTCTCAATGAATGt                   >  2:715086/1‑151 (MQ=255)
                                                                                                                                        c‑TGATTTTTAT‑GACGGCGAAAAAAA‑A‑CCGCCAGTAAAACGGCGGTGAATGCTTTCATGGAAAGAATTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTTCACTGATAAAGAATCGTTGACACAGTAGCATCAATTTTTTCAATGAATGt                   >  4:1043680/1‑151 (MQ=255)
                                                                                                                                           gATTTTTAT‑GACGGCGAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGTTTTCTCAATGAATGtt                  >  2:1068172/1‑150 (MQ=255)
                                                                                                                                            ttttttAT‑GCCGGAAAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGTTTTCTCAATGAATGTTa                 <  2:6839/149‑1 (MQ=255)
                                                                                                                                            ttttttAT‑GACGGCAAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGTTTTCTCAATGAATGTTa                 <  4:596864/149‑1 (MQ=255)
                                                                                                                                             tttttAT‑GAGGGAAAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGTTTTCTCAATGAATGTTaa                <  2:573313/150‑1 (MQ=255)
                                                                                                                                              ttttAT‑GACGGCGAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGTTTTCTCAATGAATGTTAAAc              >  4:428955/1‑151 (MQ=255)
                                                                                                                                              ttttAT‑GACGGCGAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGTTTTCTCAATGAATGTTAAAc              >  2:727480/1‑151 (MQ=255)
                                                                                                                                              ttttAT‑GACGGAAAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGTTTTCTCAATGAATGTTaaa               <  4:1349718/150‑1 (MQ=255)
                                                                                                                                               tttAT‑GACGGCGAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGTTTTCTCAATGAATGTTAAACg             >  4:479765/1‑151 (MQ=255)
                                                                                                                                                 tAT‑GACGGCGAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGTTTTCTCAATGAATGTTAAACgg            >  2:340076/1‑150 (MQ=255)
                                                                                                                                                 aaT‑GACGGAAAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGTTTTCTCAATGAATGTTAAACgg            <  2:1367548/149‑1 (MQ=255)
                                                                                                                                                       gggCAAAAAAAA‑C‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGTTTTCTCAATGAATGTTAAACGGAGCTTa      <  4:1155498/150‑1 (MQ=255)
                                                                                                                                                           gAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGTTTTCTCAATGAATGTTAAACGGAGCTTAAACt  >  2:1175794/1‑151 (MQ=255)
                                                                                                                                                           |                                                                                                                                                        
TTTTATGGCCGTACTGGGAATAATTTTATTTTCTGGTTTTCTGGCCGCGTATTTCAGCCACAAA‑TGGGATGACTAATGAACGGAG‑ATAATCCCTCACCTAACCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTAT‑GACGGCGAAAAAAA‑A‑CCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGTTTTCTCAATGAATGTTAAACGGAGCTTAAACT  >  NC_000913/1622666‑1622966

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑