Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,982,5230AG43.8% 222.6 / 43.6 137intergenic (‑136/+31)otsB/araHtrehalose‑6‑phosphate phosphatase, biosynthetic/L‑arabinose ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base A (15/62);  new base G (60/0);  total (75/62)
Fisher's exact test for biased strand distribution p-value = 5.68e-25
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 143 total aligned reads displayed.

AACGGTTCTGTCACCCGGTTCTCCTCCTTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGG‑TTGGCTA‑TGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTT  >  NC_000913/1982374‑1982672
                                                                                                                                                       |                                                                                                                                                     
aaCGGTTCTGTCACCCGGTTCTCCTCCTTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGG‑TTGGCTA‑TGGTGGGaa                                                                                                                                                      <  2:1108903/151‑1 (MQ=255)
 aCGGTTCTGTCACCCGGTTCTCCTCCTTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGG‑TTGGCTA‑TGGTGGGaaa                                                                                                                                                     <  2:1345143/151‑1 (MQ=255)
  cGGTTCTGTCACCCGGTTCTCCTCCTTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGG‑TTGGGTA‑TGGTGGGgaaa                                                                                                                                                    >  1:148093/1‑151 (MQ=255)
      tCTGTCACCCGGTTCTCCTCCTTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGG‑TTGGGTA‑TGGGGGGgaaaaa                                                                                                                                                  >  2:714927/1‑149 (MQ=255)
         ggcccccGGTTCTCCTCCTTCTCTTTTCTTATTGTGTTGCGCAAAACGCGCTTTACAGACATGTAAGTAAAGGCAGTTTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGG‑TTGGCTA‑TGGTGGGAAAAAACGCTa                                                                                                                                             <  2:879294/147‑1 (MQ=255)
              ccGGTTCTCCTCCTTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGG‑TTGGGTA‑TGGGGGGGAAAAAAGCTaaaatt                                                                                                                                        >  4:1446609/1‑148 (MQ=255)
               cGGTTCTCCTCCTTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGG‑TTGGCTA‑TGGTGGGGAAAAAAACTCAAtttt                                                                                                                                       >  2:523350/1‑149 (MQ=255)
                 gTTCTCCTCCTTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGG‑TTGGCTA‑TGGTGGGGAAAAAAGCTAAAattttg                                                                                                                                     >  1:75921/1‑151 (MQ=255)
                   tctcCTCCTTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGG‑TTGGCTA‑TGGTGGGAAAAAACGCTAAATTGTTGc                                                                                                                                    <  2:589827/150‑1 (MQ=255)
                   tctcCTCCTTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCAG‑TTGGCTA‑TGGTGGGAAAAAACGCTAAACTGTTGc                                                                                                                                    <  2:761800/150‑1 (MQ=255)
                       ctcctTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGG‑TTGGCTA‑TGGTGGGAAAAAACGCTAAATTGTTGCAGaaa                                                                                                                               <  1:308681/151‑1 (MQ=255)
                        tcctTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGG‑TTGGCTA‑TGGTGGGAAAAAACGCTAAATTGTTGCAGaaaa                                                                                                                              <  3:229110/151‑1 (MQ=255)
                         cctTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGG‑TTGGCTA‑TGGTGGGAAAAAACGCTAAATTGTTGCAGaaaaa                                                                                                                             <  4:605367/151‑1 (MQ=255)
                         cctTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGG‑TTGGCTA‑TGGTGGGAAAAAACGCTAAATTGTTGCAGaaa                                                                                                                               <  2:961621/149‑1 (MQ=255)
                           ttCTCTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGG‑TTGGCTT‑TTGGGGGGAAAAAAGCTAAATTTTTGCAGaaaaaa                                                                                                                            >  2:1187258/1‑150 (MQ=255)
                            tctcTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGG‑TTGGCTA‑TGGTGGGAAAAAACGCTAAATTGTTGCAGaaaaaa                                                                                                                            <  2:924996/149‑1 (MQ=255)
                            tctcTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGG‑GTGGGGATTGGGGGGAAAAAACCCAAATTTTTTGGGAaaaaaa                                                                                                                            >  4:96937/1‑150 (MQ=255)
                             ctctTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGG‑TTGGGTA‑TGGGGGGGAAAAAAGCTAAATTGTTGCAGAAAAAAGc                                                                                                                          >  2:421076/1‑150 (MQ=255)
                                 tttCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTTGGTTT‑TGGGGGGAAAAAAGCCAAAATTTTTGAaaaaaaaaaatca                                                                                                                       >  2:477761/1‑145 (MQ=255)
                                    cTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGG‑TTGGCCT‑TGGGGGGGAAAAAAGCCAAATTTTTGTAGAAAAAAAAATCAGAc                                                                                                                   >  2:520653/1‑150 (MQ=255)
                                      tATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGG‑GTTGGGA‑TGGGGGGGAAAAAAGCGAAAATTTTGCGGAAAAAAACAACAGAAAgg                                                                                                                >  4:1250204/1‑150 (MQ=255)
                                        ttGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGG‑TTTGGTA‑TGGGGGGGAAAAAAACCAAAATTTTTCAGaaaaaaaaaacaaacaatgg                                                                                                              >  2:932914/1‑137 (MQ=255)
                                                    gAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGG‑TTGGGTA‑TGGTGGGGAAAAAAGCTAAATTGTTTGAGGAAAAAACATCACACAAGGGGGTTTGCttttt                                                                                                  >  3:386904/1‑151 (MQ=255)
                                                     aaCGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGG‑TTGGCTA‑TGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTg                                                                                                 <  2:866126/151‑1 (MQ=255)
                                                     aaCGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGG‑TTGGCTA‑TGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTg                                                                                                 <  2:430319/151‑1 (MQ=255)
                                                     aaCGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGG‑TTGGCTA‑TGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTg                                                                                                 <  2:299427/151‑1 (MQ=255)
                                                         cgcTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGG‑TTGGCTA‑TGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTg                                                                                             <  2:1057056/151‑1 (MQ=255)
                                                           cTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGG‑TTGGGTA‑TGGTGGGGAAAAAAGCTAAAATGTTGCAGAAAAAAACATCAGACAGTGCGGTTCGCTTTTTTCTTGtt                                                                                           >  4:251056/1‑150 (MQ=255)
                                                           cTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGG‑TTGGGTA‑TGGTGGGGAAAAAAGCTAAAATGTTGCAGAAAAAAACAACAGACAGTGCGGTTTGCTTTTTTCTTGTa                                                                                           >  1:419965/1‑151 (MQ=255)
                                                           cTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGG‑TTGGGTA‑TGGTGGGGAAAAAACATAAAATGTTTCAGAAAAAAAAAACAAACAATGAGTTTTGCTTTTTTTTTGtt                                                                                           >  3:40086/1‑150 (MQ=255)
                                                           cTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGG‑TTGGCTT‑TGGTGGGGAAAAAAGCTAAATTTTTTCAGAAAAAAACATCAGACAGTGCGGTTCGCTTTTTTCttttt                                                                                           >  1:363264/1‑148 (MQ=255)
                                                            tttACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGG‑TTGGGTA‑TGGTGGGGAAAAAAGCTAAAATTTTGCAGAAAAAAACAACAGAAAGTGCGGTTTGCttttttgtttta                                                                                           >  2:1148898/1‑143 (MQ=255)
                                                             ttACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGG‑TTTGGTA‑TGGGGGGGAAAAACGCTAAAATTTTGCAGAAAAAAAAAACAGACAGGGGGGTTCGCTTTTTTCTTGTaaa                                                                                         >  1:237544/1‑150 (MQ=255)
                                                             ttACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGG‑TTGGGTA‑TGGTGGGGAAAAACGCTAAAATGTTGCAGAAAAAAACATCAGACAGGGCGGTTTGCTTTTTTTTTGtta                                                                                          >  2:1354250/1‑148 (MQ=255)
                                                              tACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGG‑TTGGGTA‑TGGTGGGGAAAAACGCTAAAATTTTGTAGAAAAAAAAATCAAACAAGGCGGTTCGCTTTTTTTTTGTaa                                                                                          >  4:924574/1‑149 (MQ=255)
                                                               aCAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGG‑TTGGCTA‑TGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACg                                                                                        <  4:1082036/150‑1 (MQ=255)
                                                                 aGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGG‑TTGGGTA‑TGGTGGGGAAAAAAACTAAAATTTTTTAGAAAAAAAAATTAAACAGTGCGGTTTGCTTTTTTCTTGTAAAGGtg                                                                                     >  4:901633/1‑150 (MQ=255)
                                                                    cATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGG‑TTTGGTA‑TGGGGGGGAAAAAAGCTAAAATTTTGCAGAAAAAAACATCAGAAAATGCGTTTTGCTTTTTTCTTTTAAAGGGCGaa                                                                                  >  1:167470/1‑151 (MQ=255)
                                                                    cATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGG‑TTGGCTA‑TTGGGGGGAAAAAAGCGAAATTGTTTCAGAAAAAAACATCAGACAGTGCGGTTTGCTTTTTGCTTGTTACGGGCgga                                                                                  >  1:203350/1‑149 (MQ=255)
                                                                     aTGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGG‑TTGGGTA‑TGGTGGGGAAAAAAACTAAAATGTTGCAGAAAAAAACAACAAACAGTGCGGTTTGCTTTTTTCTTTTAAAGGGCGAAg                                                                                 >  1:17108/1‑151 (MQ=255)
                                                                     aTGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGG‑TTGGCTT‑TGGTGGGGAAAAAAGCTAAATTGTTGTAGGAAAAAAAATCAGAAAGGGCGGTTCGCCTTTTTTTTGTTAAGGTCGa                                                                                   >  4:1385291/1‑149 (MQ=255)
                                                                      tGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGG‑TTGGGTA‑TGGTGGGGAAAAAAGCTAAAATTTTGCAGAAAAAAACATCAGACAGTGCGGTTTGCTTTTTTTTTGTAACGGTCGAAGa                                                                                >  1:436332/1‑151 (MQ=255)
                                                                        tAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGG‑TTGGCTA‑TGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATc                                                                              <  1:554361/151‑1 (MQ=255)
                                                                         aaGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGG‑TTGGCTA‑TGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCa                                                                             <  4:858986/151‑1 (MQ=255)
                                                                         aaGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGG‑GTGGGGA‑TTGTGGGGAAAAAAACTAAAATGTTGCAGAAAAAAAAAACAAACAAAGCGGTTTGCTTTTTGTTTTTTAAGGGCGaaaaaca                                                                             >  3:226815/1‑146 (MQ=255)
                                                                          aGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGG‑TTGGGTT‑TGGGGGGGAAAAAAACCAAATTTTTGCAGAAAAAAACATCAGAAAGTGGGGTTTGCTTTTTGCTTTTAACGGTCGAAGAACa                                                                             >  3:291333/1‑150 (MQ=255)
                                                                          aGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGG‑TTGGGTA‑TGGTGGGGAAAAACACTAAAATGTTGGAGAAAAAAAAATCAGACAGGGGGGTTTGCTTTTTTCTTTTTAAGGGCGAAGAtaa                                                                             >  3:198995/1‑148 (MQ=255)
                                                                          aGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGG‑TTGGGTA‑TGGTGGGGAAAAAAGCTAAAATTTTGCAGAAAAAAACATCAGAAAGTGCGGTTTGCTTTTTTCTTTTAACGGGCGAAGATCa                                                                             >  1:516956/1‑150 (MQ=255)
                                                                          aGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGG‑TTGGGTA‑TGGGGGGGAAAAAAGCTAAATTTTTGCAGAAAAAAACATCAGACAGTGCGGTTCGCTTTTTTCTTGTAAAGGTCGAAGATCa                                                                             >  1:444474/1‑150 (MQ=255)
                                                                          aGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGG‑TTGGGGA‑TGGGGGGGAAAAAAAATAAATTGTTGCAGAAAAAAACATCAGACAAGGGGGTTGGCTTTTTGTTTTTTACGGGCGaaaaacaa                                                                            >  3:423274/1‑145 (MQ=255)
                                                                          aGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGG‑TTGGCTA‑TGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCAc                                                                            <  2:146294/151‑1 (MQ=255)
                                                                          aGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGG‑TTGGCTA‑TGGGGGGGAAAAAAGCTAAATTGTTGCAGAAAAAAAAATCAGACAGTGCGTTTTGCTTTTTTTTTGTAAAGGGCGAAGATCa                                                                             >  1:78964/1‑150 (MQ=255)
                                                                          aGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGG‑TTCGCTA‑TGGGGGGGAAAAAAGCTAAATTTTTGGAGAAAAAAAAATCAGACAGAGCGTTTTGCTTTTTTTTTTTAAAGGGCGAAGATCa                                                                             >  1:196756/1‑150 (MQ=255)
                                                                          aGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGG‑GTGGGTG‑TGGTGGGGAAAAAAACAAAAATTGTGGAGAAAAAAACACCAAAAAGGGGGGTTTGCTTTTTTTTTGTTAAGGTCGAAGAACa                                                                             >  3:29769/1‑150 (MQ=255)
                                                                           gTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGG‑TTGGGTA‑TGGGGGGGAAAAAAACCAAAATTTTGCAGAAAAAAACAACAAACAGTGCGGTTTGCTTTTTTCTTTTAAAGGGCGAAGAacac                                                                            >  1:496517/1‑150 (MQ=255)
                                                                             ataGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGG‑GTGGGTA‑TGGTGGGGAAAAACGCTAAATTGTTTCAGAAAAAAACATCAGACAATGCGGTTCGCTTTTTTCTTGTTACGGTCGAAGATCACCGc                                                                         >  2:701647/1‑151 (MQ=255)
                                                                              taGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGG‑TTGGGTA‑TGGTGGGGAAAAAAGCTAAATTGTTTCAGAAAAAAACATCAGACAGTGCGTTTCGCTTTTTTCTTGTAACGGGCGAAGATAACCGCt                                                                        >  2:155757/1‑151 (MQ=255)
                                                                                 aCAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGG‑TTGGCTA‑TTGGGGGGAAAAACGCGAAAATGTTGTAGAAAAAAACAACAGACACAGCGCTTTGCCTTTTTTTTGTTAAGGGCGAAGATTACCGCTGcc                                                                     >  2:227819/1‑151 (MQ=255)
                                                                                 aCAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGG‑TTGGCTA‑TGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGcc                                                                     <  4:1127050/151‑1 (MQ=255)
                                                                                    gtgtGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGG‑TTGGCTT‑TTGGGGGGAAAAACGCTCAAATTTTTCAGAAAAAAACAACAGACAATGGGTTTTGCTTTTTTCTTTTAAACGGCGAAAATCACCCccgcccgc                                                                  >  2:939801/1‑151 (MQ=255)
                                                                                    gtgtGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGG‑TTGGCTA‑TGGGGGGGAAAAAAGCTAAATTGTTGCAGAAAAAAAAAACAGACAGTGCGGTTTGGTTTTTTCTTTTAAAGGTCGGAGATCACCGCTGcccg                                                                   >  1:71907/1‑148 (MQ=255)
                                                                                        gACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGG‑TTGGGTA‑TGGGGGGGAAAAAAACCAAAATTTTGCAGAAAAAAAAAACAAAAAGTGCGGTTTGCTTTTTTCTTTTTACGGGCGGAAATAACCCCTGCCCGCAggg                                                              >  4:186478/1‑150 (MQ=255)
                                                                                                 cGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGG‑TTGGCTA‑TGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCcg                                                      <  2:96123/150‑1 (MQ=255)
                                                                                                     aTTTGCAACGAAGAACAGCCAACCGTTATGGCGG‑TTGGGTA‑TTGTGGGGAAAAAAAATAAAAATTTGGCGAAAAAAACATCAGAAAGGGCGTTTTTCCTTTTTGTTTTAAAGGTCCAAAAGCCACCCTGCCCGGAGGATTAAGcctccga                                                  >  4:1279798/1‑145 (MQ=255)
                                                                                                        tGCAACGAAGAACAGCCAACCGTTATGGCGG‑TTGGCTA‑TGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCa                                               <  2:1446606/150‑1 (MQ=255)
                                                                                                          cAACGAAGAACAGCCAACCGTTATGGCGG‑TTGGCTA‑TGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGt                                            <  4:900165/151‑1 (MQ=255)
                                                                                                          cAACGAAGAACAGCCAACCGTTATGGCGG‑TTGGCTA‑TGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGt                                            <  4:88380/151‑1 (MQ=255)
                                                                                                          cAACGAAGAACAGCCAACCGTTATGGCGG‑TTGGCTA‑TGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGt                                            <  3:148092/151‑1 (MQ=255)
                                                                                                            aCGAAGAACAGCCAACCGTTATGGCGG‑TTGGGTT‑TGGTGGGAAAAAAAACTAAATTTTTGGCACAAAAAGCAATAAACAGGGCCATTCCCTTTTTGCTTGTCATTGTTGTAAATCACCGATGCCCGCTGGTTTTAGAcgtgcacaaaatcc                                          >  2:1464573/1‑139 (MQ=255)
                                                                                                            aCGAAGAACAGCCAACCGTTATGGCGG‑TTGGCTA‑TGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTAc                                          <  1:42180/151‑1 (MQ=255)
                                                                                                            aCGAAGAACAGCCAACCGTTATGGCGG‑TTGGCTA‑TGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTAc                                          <  4:589028/151‑1 (MQ=255)
                                                                                                            aCGAAGAACAGCCAACCGTTATGGCGG‑TTGGCTA‑TGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTAc                                          <  1:244909/151‑1 (MQ=255)
                                                                                                            aCGAAGAACAGCCAACCGTTATGGCGG‑TTGGCTA‑TGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTAc                                          <  3:553141/151‑1 (MQ=255)
                                                                                                            aCGAAGAACAGCCAACCGTTATGGCGG‑TTGGCTA‑TGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTAc                                          <  3:501044/151‑1 (MQ=255)
                                                                                                            aCGAAGAACAGCCAACCGTTATGGCGG‑TTGGCTA‑TGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTAc                                          <  2:1414187/151‑1 (MQ=255)
                                                                                                            aCGAAGAACAGCCAACCGTTATGGCGG‑TTGGCTA‑TGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTAc                                          <  3:248216/151‑1 (MQ=255)
                                                                                                            aCGAAGAACAGCCAACCGTTATGGCGG‑TTGGCTA‑TGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTAc                                          <  3:320506/151‑1 (MQ=255)
                                                                                                            aCGAAGAACAGCCAACCGTTATGGCGG‑TTGGCTA‑TGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTAc                                          <  1:563204/151‑1 (MQ=255)
                                                                                                              gaagaaCAGCCAACCGTTATGGCGG‑TTGGCTA‑TGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTg                                        <  4:1063049/151‑1 (MQ=255)
                                                                                                              gaagaaCAGCCAACCGTTATGGCGG‑TTGGCTA‑TGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTg                                        <  2:1268067/151‑1 (MQ=255)
                                                                                                                 gaaCAGCCAACCGTTATGGCGG‑TTGGGTA‑TGGGGGGGAAAAACGCTAAATTGTTGCAGAAAAAAAAATCAGACAGTGGGTTTTGCTTTTTTCTTGTAACGGTCGAAGAACACCGCTGCCAGCAGGGTTAAACCGCGAACCACGGACTgcg                                      >  1:362436/1‑150 (MQ=255)
                                                                                                                 gaaCAGCCAACCGTTATGGCGG‑TTGGCTA‑TGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTgcgc                                     <  4:534302/151‑1 (MQ=255)
                                                                                                                      gCCAACCGTTATGGCGG‑TTGGCTA‑TGGGGGGGAAAAACACCAAAATTTTGCAGAAAAAAAAATCAGACAGTGCGGTTTGCTTTTTTCTTTTAACGGTCGAAGAACACCGCCGCCCGCAGGGTTAAACCGCGGAACACGGACTGCGCGAAAg                                >  3:559325/1‑151 (MQ=255)
                                                                                                                         aaCCGTTATGGCGG‑TTGGCTA‑TGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGgag                             <  4:1187266/151‑1 (MQ=255)
                                                                                                                         aaCCGTTATGGCGG‑TTGGCTA‑TGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGgag                             <  2:86729/151‑1 (MQ=255)
                                                                                                                           ccGTTATGGCGG‑TTGGCTA‑TGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGaa                           <  4:421082/151‑1 (MQ=255)
                                                                                                                                  ggCGG‑TTGGCTA‑TGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTaa                    <  4:520657/151‑1 (MQ=255)
                                                                                                                                  ggCGG‑TTGGCTA‑TGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTa                     <  2:387233/150‑1 (MQ=255)
                                                                                                                                    cGG‑TTGGCTA‑TGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGc                  <  3:363259/151‑1 (MQ=255)
                                                                                                                                      g‑TTGGCTA‑TGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGc                  >  4:1444834/1‑149 (MQ=255)
                                                                                                                                          ggCTA‑TGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGtt             >  2:300426/1‑151 (MQ=255)
                                                                                                                                             tA‑TGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCAt          >  4:1113734/1‑151 (MQ=255)
                                                                                                                                                tggtggGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATgg        <  3:172608/151‑1 (MQ=255)
                                                                                                                                                 ggtggGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATgg        <  4:1352677/150‑1 (MQ=255)
                                                                                                                                                 ggtggGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGc       <  2:392013/151‑1 (MQ=255)
                                                                                                                                                  gtggGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCg      >  3:424292/1‑151 (MQ=255)
                                                                                                                                                   tggGAAAAAACGCTAAATTGTTGCAGAAAAAGGCATCAGACAGGGCGTTTTGCTTTTTGTTTGAAACGGCCGAAGATCACCGCTGCCAGCGGGATTAAGCGGCGAACCCCGAACGGGGCGAAAGGAGAATTATTAAGCAGGTTCAGGGCGt     >  2:248352/1‑151 (MQ=255)
                                                                                                                                                   tggGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGt     >  2:598024/1‑151 (MQ=255)
                                                                                                                                                   tggGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGt     >  2:1438035/1‑151 (MQ=255)
                                                                                                                                                      gAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGtttt  >  4:571212/1‑151 (MQ=255)
                                                                                                                                                       |                                                                                                                                                     
AACGGTTCTGTCACCCGGTTCTCCTCCTTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGG‑TTGGCTA‑TGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTT  >  NC_000913/1982374‑1982672

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑