Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,386,8620GA43.5% 135.0 / 112.1 138intergenic (+31/‑72)yfbL/yfbMputative M28A family peptidase/DUF1877 family protein
Reads supporting (aligned to +/- strand):  ref base G (11/67);  new base A (60/0);  total (71/67)
Fisher's exact test for biased strand distribution p-value = 4.12e-28
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 139 total aligned reads displayed.

CCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGAT  >  NC_000913/2386713‑2387013
                                                                                                                                                     |                                                                                                                                                           
ccGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGc                                                                                                                                                            <  4:1426681/151‑1 (MQ=255)
   cTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTTCACAAAAAACGAc                                                                                                                                                         >  4:1273906/1‑151 (MQ=255)
       ttATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATg                                                                                                                                                      <  4:65908/150‑1 (MQ=255)
         aTCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGcac                                                                                                                                                   <  1:182223/151‑1 (MQ=255)
         aTCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAAAGACaaggac                                                                                                                                                   >  2:95070/1‑146 (MQ=255)
            gTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAACGACAAGCACatt                                                                                                                                                >  1:27404/1‑151 (MQ=255)
            gTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAACGACAAGCACatt                                                                                                                                                >  1:339947/1‑151 (MQ=255)
             taataaACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCCGAAAAAAAGACAATCAAattt                                                                                                                                               >  4:1021220/1‑151 (MQ=255)
              aataaACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACattt                                                                                                                                               <  4:1380512/150‑1 (MQ=255)
                taaACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAACGACAAGCAAactttat                                                                                                                                            >  4:1388939/1‑151 (MQ=255)
                 aaaCAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCCGAAAAAACGACATGCACattttttt                                                                                                                                           >  2:323315/1‑151 (MQ=255)
                 aaaCAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACattttatt                                                                                                                                           <  4:869660/151‑1 (MQ=255)
                 aaaCAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAACGACAAGCACattttttt                                                                                                                                           >  3:300280/1‑151 (MQ=255)
                  aaCAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACattttattt                                                                                                                                          <  2:1368469/151‑1 (MQ=255)
                   aCAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACattttatttt                                                                                                                                         <  1:351689/151‑1 (MQ=255)
                    cAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTc                                                                                                                                        <  2:1244488/151‑1 (MQ=255)
                    cAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGGAAAAGGGACAAGCACatttttttttt                                                                                                                                        >  1:415652/1‑150 (MQ=255)
                      aTACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAACGACAAGCACattttttttttc                                                                                                                                       >  1:356134/1‑148 (MQ=255)
                      aTACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAAAGCCATGGCCATTTTTTTTTtcc                                                                                                                                     >  2:582242/1‑151 (MQ=255)
                          cACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCAt                                                                                                                                 <  2:1255743/151‑1 (MQ=255)
                           aCTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCCGAAAAAACGGCATGCACATTTTTTTTTTCctcctc                                                                                                                                >  2:17184/1‑151 (MQ=255)
                              tGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAACGACATGCACATTTTATTTTTCCTCATCTGt                                                                                                                             >  2:1079236/1‑151 (MQ=255)
                              tGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGTCAGAAAAAAAGACATGGACCTTTTTTTTTTCCTCATCttt                                                                                                                             >  4:1198252/1‑149 (MQ=255)
                                cccGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAAAGACAAGCACATTTTTTTTTTCCTCCTCTtttt                                                                                                                           >  1:219875/1‑151 (MQ=255)
                                 ccGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGtttt                                                                                                                          <  2:1006934/151‑1 (MQ=255)
                                 ccGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGtttt                                                                                                                          <  2:34483/151‑1 (MQ=255)
                                  cGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTa                                                                                                                         <  4:410696/151‑1 (MQ=255)
                                  cGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTa                                                                                                                         <  2:639641/151‑1 (MQ=255)
                                    gTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAg                                                                                                                       <  2:1300261/151‑1 (MQ=255)
                                          ccGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGa                                                                                                               <  4:815396/151‑1 (MQ=255)
                                          ccGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGa                                                                                                               <  2:540236/151‑1 (MQ=255)
                                            gCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGaaa                                                                                                             <  4:55354/151‑1 (MQ=255)
                                              agacagaTTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACa                                                                                                           <  4:1092463/151‑1 (MQ=255)
                                                 cagaTTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGa                                                                                                        <  2:260506/151‑1 (MQ=255)
                                                 cagaTTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGa                                                                                                        <  4:1327568/151‑1 (MQ=255)
                                                   gaTTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCCGAAAAAAAGACCAGCACATTTTTTTTTTCCTCATCTGTTTTTAGATGCGAAAAATGaa                                                                                                       >  4:967341/1‑150 (MQ=255)
                                                     ttGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAACGAAAAGCACATTTTATTTTTTCTCATCTGTTTTTAAATTCGAAAAACGaaaat                                                                                                    >  4:477313/1‑149 (MQ=255)
                                                      tGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCCGAAAAAGAGGAACGAACATTTTATTTTTCCTCATCTCTTTTTATATGCGAAAAAATAAAttt                                                                                                   >  2:276319/1‑150 (MQ=255)
                                                              cAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACggagg                                                                                          <  2:567594/151‑1 (MQ=255)
                                                              cAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACggagg                                                                                          <  2:724129/151‑1 (MQ=255)
                                                                gAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGaa                                                                                        <  2:1428024/151‑1 (MQ=255)
                                                                    aTGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAaaat                                                                                    <  4:1426404/151‑1 (MQ=255)
                                                                      ggCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAaaata                                                                                   <  2:375693/150‑1 (MQ=255)
                                                                         tCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATg                                                                               <  3:128299/151‑1 (MQ=255)
                                                                           aGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGGAACATGAAATTCAACGGAGGAAAAATAAATggg                                                                             <  2:216049/151‑1 (MQ=255)
                                                                           aGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAAAGACATGCACATTTTTTTTTTTCTCCTCTGTTTTTAAAAGCGAAACAAGAAATTTAAAGGAGGAAAAATAAAAggg                                                                             >  2:142751/1‑151 (MQ=255)
                                                                            ggTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAACGACATGCACATTTTTTTTTTCCTCCTCTGTTTTTAGATGCGGAAAATGAAAATCAACGGGGGGAAAATAAATgggg                                                                            >  1:224467/1‑150 (MQ=255)
                                                                             gTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGaa                                                                           <  4:405711/151‑1 (MQ=255)
                                                                             gTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAAAGACATGAACATTTTTTTTTTCCTCATCTGTTTTTAAATGCGAAACATGAAAATCAAAGGAGGGAAAATAAATTGGaa                                                                           >  3:252756/1‑151 (MQ=255)
                                                                             gTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGTCCGAAAAAAAGAAAAGAACAATTTTTTTTTTCTCATCTGTTTTTAGAAAAGAAAAAAGAAAATTAAAGGAGGGAAAAAAAAATGGGa                                                                            >  3:350625/1‑151 (MQ=255)
                                                                               aGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAAt                                                                          <  2:246840/150‑1 (MQ=255)
                                                                               aGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATg                                                                         <  4:46623/151‑1 (MQ=255)
                                                                                 tGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATg                                                                         <  1:477140/149‑1 (MQ=255)
                                                                                     tGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCCGAAAAAACGACATGCCCCTTTTATTTTTCCTCCTCTTTTTTTAAATGCGAAAAATGAAAATTAAAGGAGGGAAAAAAAATGGGGATGATTggg                                                                   >  2:1437703/1‑150 (MQ=255)
                                                                                       gtgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTTCAGAAAAAAAGACAAGCACAATTTATTTTTTCTCATCTGTTTTTAAATGCGGAAAAAGAAAATTAAAGGAGGAAAAATAAATTGGGATGATTGGCtt                                                                 >  3:99/1‑150 (MQ=255)
                                                                                       gtgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCCGAAAAAACGACATGCACATTTTTTTTTTCCTCCTCTGTTTTTAGATGCGGAACATGAAAATTTACGGGGGGAAAAAAAATGGGGATGATTGGGTa                                                                 >  1:555317/1‑151 (MQ=255)
                                                                                       gtgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCCGAAAAAACGACAAGCACAATTTATTTTTCCTCCTCTGTTTTTAGATGCGAAACATGAAAATTAACGGAGGGAAAATAAATTGGGATGATTGGCTa                                                                 >  1:265001/1‑151 (MQ=255)
                                                                                       gtgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCCGAAAAAAAGAAATGCACATTTTATTTTTCCTCATCTGTTTTTAGAAGCGAAAAATAAAATTTAAAGGAGGAAAAAAAAATTGGGAAGAtttggta                                                                 >  3:398476/1‑146 (MQ=255)
                                                                                       gtgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCt                                                                  <  3:497117/150‑1 (MQ=255)
                                                                                       gtgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAACGACAAGCACATTTTTTTTTTCCTCCTCTGTTTTTAGAAGCGAAAAATGAAATTTAAAGGAGGGAAAATAAATGGGGATGAtttggta                                                                 >  1:478040/1‑146 (MQ=255)
                                                                                       gtgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAACGAAATGGACATTTTTTTTTTCCTCATCTGTTTTTAGATGGGAAAAAAGAAAATTAAAGGGGGGAAAAAAAATAGGGATGAtttggta                                                                 >  1:451398/1‑146 (MQ=255)
                                                                                       gtgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAAAGACATGCACATTTTTTTTTTCCTCATCTGTTTTAAAAAGGGAAAAAAGAAAATCTACGGAGGAAAAATAAATGGGAATGATTgggtt                                                                 >  1:143969/1‑148 (MQ=255)
                                                                                       gtgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGATCCAGAAAAAAAGACAAGAAAATTTTATTTTTTCTCATCTGTTTTTAGAAGGGAAAAAAGAAAATCAAAGGGGGAAAAAAAAATAGGAATGATTGGGTa                                                                 >  3:57109/1‑151 (MQ=255)
                                                                                        tgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTAt                                                                <  3:225768/151‑1 (MQ=255)
                                                                                           tataACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCCGAAAAAACGACAAGCACATTTTTTTTTTCCTCCTCTGTTTTTAGATGCGAAACATGAAATTTAAAGGAGGAAAAAAAAATAGGGAAGATTTGCTAtttt                                                             >  2:1367417/1‑151 (MQ=255)
                                                                                            ataACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCCGAAAAAAAGACAAGCACATTTTTTTTTTCCTCATCTGTTTTTAGATGCGAAAAAAGAAATTCAACGGAGGAAAAAAAAAAGGGGATGATTGGCTAttttt                                                            >  2:183154/1‑150 (MQ=255)
                                                                                             taACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGc                                                           <  3:497035/151‑1 (MQ=255)
                                                                                                 tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAAAGACAAGCACATTTTATTTTTTCTCCTCTGTTTTTAAATGGGAAAAAAGAAAATTAAAGGAGGGAAAATAAAATGGGATGATTGGCTATTTTTCAGa                                                        >  4:88877/1‑150 (MQ=255)
                                                                                                  ttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGa                                                        <  4:1212097/149‑1 (MQ=255)
                                                                                                  ttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGa                                                        <  2:449162/149‑1 (MQ=255)
                                                                                                  ttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAACGACAAGCACATTTTTTTTTTCCTCATCTGTTTTTAGATGCGAAACATGAAAATCAACGGAGGAAAAATAAATGGGGATGATTTGCTTTTTTTCAGaaa                                                      >  1:524319/1‑151 (MQ=255)
                                                                                                    gTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTGCAGGAAAAAAGAAAAGCAAATTTTTTTTTTTCTCATCTGTTTTAAGAAGGGAAACAAGAAAATTAAAGGGGGGAAAAAAAAAAGGAAAGATTGGGTATTTTTTAGaaaat                                                    >  4:97480/1‑149 (MQ=255)
                                                                                                    gTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCCGAAAAAAAGACATGGACATTTTATTTTTCCTCATCTGTTTTTAGATGCGAAACAAGAAATTCAAAGGAGGAAAAATAAATGGGGATGATTGGCTATTTTTTAGaaaat                                                    >  4:1404963/1‑149 (MQ=255)
                                                                                                        taCAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAAAGACAAGAACAATTTTTTTTTCCTCATCTTTTTTTAAATGAGGAAAAATAAAATCAAAAGAGGGAAAAAAAAATGGGATAATTTGCGAttttttaaaaaatgaat                                                >  4:4731/1‑138 (MQ=255)
                                                                                                        taCAAAAGTAAATAATAAAAATTTCTCGAGGCGGGGACCGGAAAAGAAAAATGCAGAATTTATTTTCTCTTCGTATTTTTTAAATGCGGAAAAATCAAATATACGGGGGGGAAAAAAAAAGGGAAGGTTTGCCTGTTTTTAGGAAATGGAc                                                 >  4:119270/1‑151 (MQ=255)
                                                                                                         acaacaGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTc                                               <  4:744126/151‑1 (MQ=255)
                                                                                                         acaacaGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTc                                               <  2:497499/151‑1 (MQ=255)
                                                                                                           aacaGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAg                                             <  2:1415438/151‑1 (MQ=255)
                                                                                                            acaGTAAATAATAAAAATTTCTCGAGGCGGGTTCACAAAAAAGGGAATGCCCATTTTATTTTTCCTCCTCTGTTTTAAGAAGCGGAAAATGAAATTTAACGGAGGGAAAAAAAATTGGGATGATTTGGTATTTTGTTGAAAATGAATctg                                             >  2:564284/1‑148 (MQ=255)
                                                                                                            acaGTAAATAATAAAAATTTCTCGAGGCGGGTCCCGAAAAAACGACATGCACATTTTTTTTTTTCTCCTCTGTTTTTAGATGCGAAAAATGAAATTCAAAGGAGGAAAAATAAATGGGGATGATTGGCTATTTTGCAGAAATTGACTCAGa                                            >  3:470720/1‑151 (MQ=255)
                                                                                                            acaGTAAATAATAAAAATTTCTCGAGGCGGGTCCCGAAAAAACGACATGCACATTTTTTTTTTCCTCATCTGTTTTTAAATTCGAAAAATGAAAATCAACGGAGGAAAAATAAATTGGAATGATTTGCTATTTTTCAGAAATTTACTCaaa                                            >  1:163166/1‑149 (MQ=255)
                                                                                                            acaGTAAATAATAAAAATTTCTCGAGGCGGGGCCCGAAAAAACGACATGCACATTTTTTTTTTCCTCCTCTGTTTTTAAATGCGAAAAATGAAATTCAACGGAGGGAAAAAAAATGGGGATGATTGGGTATTTTTTAGAAAATGAATCAGa                                            >  1:7937/1‑151 (MQ=255)
                                                                                                            acaGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAAAGACATGCACATTTTTTTTTTCCTCATCTTTTTTTAGATGCGAAAAATGAAATTCAACGGAGGAAAAAAAAATGGGGATGATTGGCTATTTTTCAGAAAATTACTCAGa                                            >  1:539049/1‑151 (MQ=255)
                                                                                                             caGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGaa                                           <  4:1430134/151‑1 (MQ=255)
                                                                                                             caGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAACGACATGCACATTTTTTTTTTCCTCATCTGTTTTTAAAAGCGAAAAAAGAAAATTAACGGGGGAAAAAAAAAAGGGGAAGATTGGCTCTTTTTCAGAAAATGACTCAGa                                            >  2:778228/1‑150 (MQ=255)
                                                                                                               gTAAATAATAAAAATTTCTCGAGGCGGGTCCCGAAAAAGCGACATGCACATTTTTTTTTTCCTCCTCTGTTTTTAGATGCGAAACATGAAAATCAACGGAGGGAAAATAAATGGGAATGATTGGCTATTTTTCAGAAATTTAATCAGaaa                                          >  2:221433/1‑150 (MQ=255)
                                                                                                               gTAAATAATAAAAATTTCTCGAGGCGGGTCCCGAAAAAAAGACAAGCACATTTTTTTTTTCCTCATCTGTTTTTAGATGCGGAAAATGAAAATTAAAGGAGGGAAAATAAATTGGGATGATTTGGTCTTTTTTAAAAAATGAATCCGaaaa                                         >  3:374713/1‑151 (MQ=255)
                                                                                                                tAAATAATAAAAATTTCTCGAGGCGGTGCCAGAAAAAGAGACATGCAAATTTTATTTTTCCTCATTTGTTTTTAGATGCGAAAAATGAAAATTAACGGAGGAAAAAAAAATTGGAATGATTTGCTATTTTTCAGAAAATGAATCCGaaaa                                         >  4:567660/1‑150 (MQ=255)
                                                                                                                     aataaAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTaa                                   <  4:715102/151‑1 (MQ=255)
                                                                                                                          aaaTTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAAc                              <  2:676969/151‑1 (MQ=255)
                                                                                                                                  cGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGTGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAg                      <  3:543380/151‑1 (MQ=255)
                                                                                                                                  cGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTg                        <  4:1079232/149‑1 (MQ=255)
                                                                                                                                  cGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAg                      <  2:1021193/151‑1 (MQ=255)
                                                                                                                                   gAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGt                     <  2:1308244/151‑1 (MQ=255)
                                                                                                                                      gCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACGCAGAAAAGATTAACCAACTGCTTGAGTc                    <  3:20910/149‑1 (MQ=255)
                                                                                                                                             cAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGaaaa           <  4:1004084/151‑1 (MQ=255)
                                                                                                                                             cAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGaaa            >  2:851088/1‑150 (MQ=255)
                                                                                                                                                aaaaaGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAAcc        <  1:112658/151‑1 (MQ=255)
                                                                                                                                                   aaGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCttt     >  4:1457323/1‑151 (MQ=255)
                                                                                                                                                     gCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGAAAAAATAAATGGGAATGATTGGCAATTTTGCAAAAATTGACTCAGAAAAGATTAACCAACGGCTGGAGTCTACGGAAAACCTTTGAt  <  4:17184/151‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                           
CCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGAT  >  NC_000913/2386713‑2387013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: