Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,434,8820TG44.6% 189.4 / 30.7 121Y252S (TAC→TCC) truAtRNA pseudouridine(38‑40) synthase
Reads supporting (aligned to +/- strand):  ref base T (21/46);  new base G (54/0);  total (75/46)
Fisher's exact test for biased strand distribution p-value = 2.27e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 121 total aligned reads displayed.

TCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCAT  >  NC_000913/2434737‑2435030
                                                                                                                                                 |                                                                                                                                                     
tCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATcc                                                                                                                                                  <  3:30111/151‑1 (MQ=255)
   atatTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCAcc                                                                                                                                               <  2:152509/151‑1 (MQ=255)
   atatTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGCTTTTGAAGATCATACCGGGCAAGGTAAaccacc                                                                                                                                               >  4:1141672/1‑151 (MQ=255)
   atatTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGCTTTTGAAGATAATACCCGTCCAGGGAATTCAc                                                                                                                                                >  3:64962/1‑150 (MQ=255)
    tatTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGAGCATACCGGGCAGGGGAAaccaccc                                                                                                                                              >  4:1286615/1‑150 (MQ=255)
     atTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCCGGCAGGGGAATCCAcccc                                                                                                                                             >  2:429053/1‑149 (MQ=255)
       ttGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGTCAGGGGAATCCACcccg                                                                                                                                            >  4:611978/1‑150 (MQ=255)
        tGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATAACGGTCAGGGGAATCCACCGCGa                                                                                                                                           >  1:104344/1‑150 (MQ=255)
        tGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAAAACATAACGGTCAGGGGAATTCACCACGAc                                                                                                                                          >  2:585772/1‑151 (MQ=255)
          ccTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGAcc                                                                                                                                         <  1:221159/150‑1 (MQ=255)
          ccTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCa                                                                                                                                        <  4:454131/151‑1 (MQ=255)
             aTGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGTTCATACCGGTCGGGGTAATCCACCGCGACCAgg                                                                                                                                      <  4:516044/150‑2 (MQ=255)
             aTGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGAt                                                                                                                                     <  1:377479/151‑1 (MQ=255)
               gCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCCGGCAGGGGAATCCACCGCGACCAGATaa                                                                                                                                   >  3:381357/1‑150 (MQ=255)
               gCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATCATACCGGTCAGGGGAATCCACCGCGACCCGATa                                                                                                                                    >  3:387830/1‑150 (MQ=255)
                 cTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATCATACCGGGCAGGGGAATCCACCGCGACCAGATACAg                                                                                                                                 >  1:210993/1‑151 (MQ=255)
                    gTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGTCAGGGGAATCCACCCCGACCAGATACAGccc                                                                                                                              >  4:353208/1‑151 (MQ=255)
                      aCTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCtt                                                                                                                            >  2:337811/1‑151 (MQ=255)
                      aCTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGGAATCCACCGCGACCAGATACAGCCCtt                                                                                                                            >  2:329870/1‑151 (MQ=255)
                         cGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTcc                                                                                                                          <  2:874771/150‑1 (MQ=255)
                         cGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCg                                                                                                                         <  3:297904/151‑1 (MQ=255)
                            cATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATCATACCGGTCAGGGGAATCCACCGCGACCAGATACAGCCCTTCCG‑Ctt                                                                                                                     >  4:1448853/1‑151 (MQ=255)
                                 ttCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGAACATACCGGTCAGGGGAATCCACCGCGACCAGATACAGCCCCTCCG‑CCTTTGcc                                                                                                                >  2:51477/1‑151 (MQ=255)
                                      gCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTtgc                                                                                                           <  4:1295882/151‑1 (MQ=255)
                                      gCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGGAATCCACCGCGACCCGATACAGCCCTTCCG‑CTTTTGCCGtttc                                                                                                           >  4:639477/1‑149 (MQ=255)
                                         gTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGGAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTtgctgc                                                                                                        >  2:379468/1‑151 (MQ=255)
                                         gTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTGTTTGCAAATCACACCGGTCCCGGGCATCCACCGCGAACCGATAAAGTCCTTTCC‑GTTTTGGCGttcatgc                                                                                                        >  4:390912/1‑146 (MQ=255)
                                          tAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGTCAGGGGAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTTCCGTTGCTgcc                                                                                                       >  4:294628/1‑151 (MQ=255)
                                           aaCTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTTCCGTTGCTgccc                                                                                                      >  2:592378/1‑150 (MQ=255)
                                               aCGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGTCCGGGGAATCCACCGCGACCCGATACAGCCCTTCCG‑CTTTTTCCGTTGCTgccccccg                                                                                                  >  4:25986/1‑149 (MQ=255)
                                               aCGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCa                                                                                                   <  2:1330383/150‑1 (MQ=255)
                                               aCGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGGAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTTCTgccgcccg                                                                                                  >  1:259107/1‑149 (MQ=255)
                                                   tGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGGCGTTGCTGCCGCCAGCGtt                                                                                              >  2:555703/1‑151 (MQ=255)
                                                   tGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATAACGGGCAGGGGAAACCACCGCGACCAGAGACACCCCTTTCC‑CTTTTGCCGGTGCTCCCCCCCGCGtt                                                                                              >  2:935449/1‑151 (MQ=255)
                                                     gCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTc                                                                                             <  1:346011/150‑1 (MQ=255)
                                                     gCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCt                                                                                            <  2:180318/151‑1 (MQ=255)
                                                       tACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGGAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGGTGCTGCCGCCAGCGTTCTGt                                                                                          >  2:238997/1‑151 (MQ=255)
                                                         cAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGTCAGGGGAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGGTGCTGCCGCCCGCGTTCTGTc                                                                                         >  3:305145/1‑150 (MQ=255)
                                                          aGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGGAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTTCCGTTGCTGCCGCCAGCGTTCTGTCCt                                                                                       >  2:631430/1‑151 (MQ=255)
                                                                aaaTGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGAACCTACCGGTCCGGGTAAATCACCGCGACCAGAAACAACCCTTCCG‑CTTTTTCCGTTGCTGGCGCCCGCGCTCTGTCCTTTtccg                                                                                 >  4:824705/1‑151 (MQ=255)
                                                                  aTGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCCGGCAGGGGAATCCAACGCGACCCGATACAGCCCTTCCG‑CTTTTTCCGTTTCTGCCGCCCGCGGTccggtcggaagagcg                                                                               >  4:851852/1‑137 (MQ=255)
                                                                  aTGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTc                                                                                             <  2:851861/137‑1 (MQ=255)
                                                                    gTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATTAAACCGGCTAGGGGAATCCACCGCGACCCGAAAAAACCCTTCCG‑CCTTTGTCGTTCTTCCCGCCAACGGTCTGTCCTTTtccgcc                                                                               >  2:1247280/1‑149 (MQ=255)
                                                                    gTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGGAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTgccgcc                                                                               >  2:878575/1‑149 (MQ=255)
                                                                    gTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATAACCGGCAGGGGAATCCCCCCCGACCAGAAACCGACCTTCCC‑CTTTTTCCGTTGCTTCCCCCCGCCTTCTGTTCTTTtccgcccg                                                                             >  3:453758/1‑149 (MQ=255)
                                                                       ccGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCa                                                                           <  2:1302080/150‑1 (MQ=255)
                                                                           gCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGGAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCCGCGTTCTGTCCTTTGCCGCCAGCAAct                                                                        >  1:546183/1‑149 (MQ=255)
                                                                            cATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTg                                                                     <  4:1320097/151‑1 (MQ=255)
                                                                                 ttCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCGGTGAAGGTAATCCACCCCGACCAGAAACAGCCCTTCCG‑CTTTTTCCGTTGCTGCCGCCCGCGGTCTGTCCTTTTCCGCCCGCAACTCTTCTATc                                                                >  4:853802/1‑151 (MQ=255)
                                                                                  tctcGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATCATACCGGTCAGGGGAATCCACCCCGACCAGATACAACCCTTCCG‑CTTTTGCCGTTGCTGCCCCCAGCGTTCTGTCCTTTTCCGCCAGCAACTCTGCTATcc                                                               >  2:281647/1‑151 (MQ=255)
                                                                                    tcGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGTCAGGGGAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAg                                                             >  1:16376/1‑151 (MQ=255)
                                                                                    tcGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCCGTCAGGGGAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTTTCCTTTGCCGCCAGCAACTCTGCTATCCac                                                             >  2:1021696/1‑150 (MQ=255)
                                                                                     cGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATcc                                                               <  4:441195/148‑1 (MQ=255)
                                                                                       ttAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGTCAGGGGAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGctc                                                          >  2:429474/1‑151 (MQ=255)
                                                                                       ttAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGGAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCCGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGctc                                                          >  2:316752/1‑151 (MQ=255)
                                                                                          gTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGAGCATAACGGTCAGGGGAATACACCGCGGCCAGATACAGCCCCTCCG‑GTTTTGCCCGTGCTGGCCCCCGCGTTCTGGCCCTTGCCGCCAGCCACTCTGGTATCCACCTCTcc                                                       >  4:196287/1‑151 (MQ=255)
                                                                                          gTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTcc                                                       <  2:145676/151‑1 (MQ=255)
                                                                                          gTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGGAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTcc                                                       >  2:704685/1‑151 (MQ=255)
                                                                                            ccGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCgg                                                     <  4:1220757/151‑1 (MQ=255)
                                                                                               ccAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATCATAACGGTCAAGGGAATTCATCCCGACCCGATAAAGCCCTTCCGACTTTTG‑CGTTTCTACCCCCGGCGGTCTGTCCTTTGCCGCCAGCAACTCTTCTATCCAGCCCTTCGGGTg                                                  >  4:1145130/1‑151 (MQ=255)
                                                                                                 aGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCCTCCG‑CTTTTTCCGCTGCTGCCGCCAGCGGTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTggg                                                >  2:434067/1‑150 (MQ=255)
                                                                                                  gAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGGAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGtt                                               >  1:103996/1‑151 (MQ=255)
                                                                                                   aaaTAGCGGGCCCATTGGCGGTTTTGGGAGATCGTACCGGTCAGGGGAAACCACCGCCGCCAGATAAAGACCCTTCG‑CGTTTTCCGGTTGTGCCCCCACCGTTCTGTCCTTTGCCGCCAGCAACCCTGTTATCCAGCTCTTCCGCTgggtt                                              >  4:1379655/1‑148 (MQ=255)
                                                                                                   aaaTAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGGAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTTCCGTTGCTGCCGCCAACGTTCTGTCCTTTGCCGCCCGCAACTCTGCTATCCAGCTCTTCGGCTGGGtg                                              >  4:593518/1‑151 (MQ=255)
                                                                                                     aTAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCGGGCAGGGGAATCCACCGCGACCCGATACAGCCCTTCCG‑CTTTTTCCGTTGGTGCCGCCCGCGGTCTGTCCTTTGCCGCCCGCAACTCTGCTATCCAGCTCTCCCGCTGGGtgtg                                            >  4:938764/1‑151 (MQ=255)
                                                                                                     aTAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGGAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTTCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGGtgtg                                            >  2:962286/1‑151 (MQ=255)
                                                                                                       aGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGGAATCCACCGCGACCAGATAAAGCCCTTCCG‑CTTTTTCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCCGCTGGTTGTggg                                          >  2:901307/1‑151 (MQ=255)
                                                                                                         cGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGc                                         <  3:564299/150‑1 (MQ=255)
                                                                                                         cGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGc                                         <  1:66043/150‑1 (MQ=255)
                                                                                                         cGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCa                                        <  2:742027/151‑1 (MQ=255)
                                                                                                           ggCCCATTGGCGGTTTTGGAAGATCATACCGGGCAGGGGAATCCACCGCGACCAGATACAGCCCCTCCG‑CTTTTTCCGGTGCTGCCGCCAGCGGTCTGTCCCTTGCCGCCAGCAACTCTGCTATTCCGCTCTCCGGCTGGTTGTGGGCAcc                                      >  4:481455/1‑151 (MQ=255)
                                                                                                           ggCCCATTGGCGGTTTTGGAAGATCATACCGGGCAGGGGAAACCACCCCGACCAGATACAGCCCTTCCC‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGGTGTGGGCAcc                                      >  4:1108440/1‑151 (MQ=255)
                                                                                                                  tGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTcc                               >  2:1183078/1‑151 (MQ=255)
                                                                                                                     cGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCAt                             <  3:462176/150‑1 (MQ=255)
                                                                                                                      ggTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCa                           <  4:1217896/151‑1 (MQ=255)
                                                                                                                       gTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATc                            >  4:378236/1‑149 (MQ=255)
                                                                                                                         tttGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAgg                         <  4:308687/150‑1 (MQ=255)
                                                                                                                         tttGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGc                        >  2:499614/1‑151 (MQ=255)
                                                                                                                          ttGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGc                        <  2:1228785/150‑1 (MQ=255)
                                                                                                                          ttGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCt                       >  4:1170052/1‑151 (MQ=255)
                                                                                                                           tGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTg                      <  1:291189/151‑1 (MQ=255)
                                                                                                                             gAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGcc                    >  4:1357454/1‑151 (MQ=255)
                                                                                                                                 aTCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACa                <  2:387106/151‑1 (MQ=255)
                                                                                                                                   cATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAat              <  2:1287349/151‑1 (MQ=255)
                                                                                                                                      aCCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATAtt           >  2:1273590/1‑151 (MQ=255)
                                                                                                                                        cGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTcc         >  4:232443/1‑151 (MQ=255)
                                                                                                                                        cGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCG‑CCTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTcc         >  2:56210/1‑151 (MQ=255)
                                                                                                                                           tCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTg       <  1:497569/150‑1 (MQ=255)
                                                                                                                                            cAGGGTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGAc     <  2:1218618/151‑1 (MQ=255)
                                                                                                                                            cAGGGTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGAc     <  4:591246/151‑1 (MQ=255)
                                                                                                                                            cAGGGTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGAc     <  2:1099795/151‑1 (MQ=255)
                                                                                                                                              gggTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCa   >  3:352030/1‑151 (MQ=255)
                                                                                                                                              gggTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCa   >  3:393053/1‑151 (MQ=255)
                                                                                                                                              gggTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCa   >  3:69195/1‑151 (MQ=255)
                                                                                                                                               ggTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGAcc    <  3:133822/149‑1 (MQ=255)
                                                                                                                                               ggTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGAcc    <  1:397070/149‑1 (MQ=255)
                                                                                                                                               ggTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGAc     <  3:16376/148‑1 (MQ=255)
                                                                                                                                               ggTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGAc     <  1:492706/148‑1 (MQ=255)
                                                                                                                                               ggTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCat  <  2:611978/151‑1 (MQ=255)
                                                                                                                                               ggTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCat  <  4:592377/151‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                     
TCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCAT  >  NC_000913/2434737‑2435030

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑