Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,603,3420AC45.3% 133.8 / 113.4 117T509P (ACC→CCC) hyfBhydrogenase 4, membrane subunit
Reads supporting (aligned to +/- strand):  ref base A (60/4);  new base C (0/53);  total (60/57)
Fisher's exact test for biased strand distribution p-value = 5.40e-29
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 117 total aligned reads displayed.

GCGCAGTACACACGCTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGGCTGGCACCGAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGTTGCTGTTACTACTGGCGATGCCTTTGCTGCCTGGCCTTTACTGGCTGTGGTGTCGTTCGCGCCGCGC  >  NC_000913/2603194‑2603487
                                                                                                                                                          |                                                                                                                                                 
gcgcAGTACACACGCTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGGCTGGCACCGAAGATAATGCATATTGCCCATGCGTTTACCAATAc                                                                                                                                                  <  3:267302/150‑1 (MQ=255)
     gTACACACGCTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGGCTGGCACCGAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCTcc                                                                                                                                            <  2:1111378/151‑1 (MQ=255)
     gTACACACGCTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGGCTGGCACCGAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCTc                                                                                                                                             >  3:242472/1‑150 (MQ=255)
          cacGCTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGGCTGGCACCGAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCTCCCGcc                                                                                                                                        >  4:1427402/1‑150 (MQ=255)
           acGCTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGGCTGGCACCGAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCTCCCGCCa                                                                                                                                       >  1:71060/1‑150 (MQ=255)
           acGCTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGGCTGGCACCGAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCTCCCGCCAc                                                                                                                                      <  1:104616/151‑1 (MQ=255)
            cGCTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGGCTGGCACCGAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCTCCCGCCa                                                                                                                                       >  1:313884/1‑149 (MQ=255)
            cGCTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGGCTGGCACCGAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCTCCCGCCa                                                                                                                                       >  1:329409/1‑149 (MQ=255)
            cGCTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGGCTGGCACCGAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCTCCCGCCa                                                                                                                                       >  3:143251/1‑149 (MQ=255)
             gCTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGGCTGGCACCGAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCTCCCGCCACTg                                                                                                                                    >  4:718873/1‑151 (MQ=255)
              cTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGGCTGGCACCGAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCTCCCGCCa                                                                                                                                       >  3:200477/1‑147 (MQ=255)
              cTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGGCTGGCACCGAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCTCCCGCCACTGt                                                                                                                                   >  2:213479/1‑151 (MQ=255)
                gAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGGCTGGCACCGAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCTCCCGCCACTGTCg                                                                                                                                 >  3:461374/1‑151 (MQ=255)
                    agGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGGCTGGCACCGAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCTCCCGCCACTGTCGcc                                                                                                                               >  2:88620/1‑149 (MQ=255)
                          aGGAAGCGCAAAATACGTTGTTCGTCGCCATCCTCTCCCCCGCGGCCTTTTGCTTATTATTTGCGTTTCGTCCCAGTTGGCTGCCACCGAAGATAATCAATATTGCCATTGTTTTCACCAATCCCCCTCCCGCCACTGTCGCCAGCGGAAt                                                                                                                       <  4:110575/151‑1 (MQ=255)
                                tGCCAAATACTATGATCGCCCCCATGCTCCTCCTCGCGGCACTCTGCTTATTAATTGCGCTTAGTCCCGTTTGGCTGGCACCGAAGATAATCAATTTTGCCTATGCTTTAACCAATCCCCCTCCCGCCACTGTCGCCAGCGGAATAGCAc                                                                                                                  <  1:510305/150‑1 (MQ=255)
                                   aaaaTACGTTGATCGTCGCCTTGCTTCTGCCCGCGCCCCTCTGCTTATTATTTGCGTTTAGTGCCGGTTGGCGGCCCCAAAAGATAATGCATTTTCCCCTTGTTTTTACAAATCCCCCCCCCGCCACTGTCGCCAGCGGAATAGCACTTGt                                                                                                              <  2:140023/150‑1 (MQ=255)
                                    aaaTAAGATGATCGTCGCCTTGCTCCCGCTCGCGCCTCTTGGTGTATAATTTCCTATAAGGCCTAGTTGCCGGCCACAGAAGAAAATGCATATCGCCCATGTTTTTACCAACCCCCCCCCCGCCACTGTCGCCAGCGGAATAGCACTTGTa                                                                                                             <  2:422037/151‑1 (MQ=255)
                                      aTACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGGCTGGCACCGAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTAcc                                                                                                           >  2:111216/1‑151 (MQ=255)
                                       tatgttgacCGCCGCCTTTCTCCTCCTCGCGCCTCTCTGATTATTATTTGCGTTTAGGGCCGGTTGGCTGCCACAAAAAATAATGAATATTCCCCATGCTTTTCCCAACCCCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTAccc                                                                                                          <  3:444338/146‑1 (MQ=255)
                                       tACGATGATCGCCGCCATGCTCCTGCTCGCGGCACTCTGCGTTTTAATTGCGTTTAGTGCCAGTGGGCTGGCACCAAAAATAATGCATATTGCCCATGCTTTTACCAATCCCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTAccc                                                                                                          <  1:128738/151‑1 (MQ=255)
                                        aCGATGTTCGTCCCCATGCTCCTGCCCGCGGCACTGTGTGTATTATTTGCGTTTGGTGCCATTTGGGGGGCACGAAAAATAACACATTTGCCCTATGTGTTTCCCACCCCCCCCCCCGCCACTGTCGCCAGCGGAATAGCACTTGTAccc                                                                                                          <  4:898411/150‑1 (MQ=255)
                                        aCGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGGCTGGCACCGAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCg                                                                                                         >  2:570492/1‑151 (MQ=255)
                                        aCGATGATCCCCGCTATCCTCCTGCCCGCGCCACTCTGCGTATTACTTGCGCTTGGTGCCAGTGGGGTGGCCCGAAAAATAAGGCATATCCCCCTTCCTTTACCCATTCCCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTAccc                                                                                                          <  2:764162/150‑1 (MQ=255)
                                          gatgatCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTCAGTGCCAGTTGGCTGGCACCGAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGc                                                                                                       >  4:105117/1‑151 (MQ=255)
                                             gtccgtccCCTTTCTTCTGCCCGCGGCCCTTTGTTTATTATTTGCTTTTAGTGCTGTTGGGCTGGCACCAAAAATAATGCTTTTTCCCCTTGCTTTTCCCAATCCCCCCCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCAc                                                                                                     <  2:1058485/147‑1 (MQ=255)
                                              atCGCCCCCATGCTCCTGCCCGCGCCCCTCTGCGTATTAATTGCGTTTGGTGCCTGTGGGCTGGCACAAAAGAAAATGAATATTCCCCATGCGTTTACCAATCCCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGt                                                                                                   <  2:847003/151‑1 (MQ=255)
                                               tcgtcgCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGGCTGGCACCGAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGtt                                                                                                  >  2:274343/1‑151 (MQ=255)
                                                  ccgCCTTGCTCCTCCCCGCGGCACTCTGTTTATTATTTGCTTTTGGTCCCGGTTGGCGGCCACAAAAAAAAATGAATTTCCCCCTTGCTTTCACCACCCCCCCCCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCa                                                                                               <  4:1320312/150‑1 (MQ=255)
                                                   cgCCATGCTCCTGCCCGCGCCCCTCTGCTTTTTAATTGCTTTTGGTCCATGTTGGCGGCCCCCAAAGAAAATGTTTTTTCCCCATGCTTTTCCCATTCCCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCAt                                                                                              <  2:212820/151‑1 (MQ=255)
                                                   cgCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGGCTGGCACCGAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCAt                                                                                              >  2:1387853/1‑151 (MQ=255)
                                                   ccccATTCTCCTGCGCGCGCCTCTCTGCGTATTCTTTTCCTTTAGTGCTGGTTGGTCGGCACGAAAAAAAATCAATATTCCCCTTTCTTTTCCCAACCCCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCa                                                                                               <  2:712541/148‑1 (MQ=255)
                                                     ccgtcctccTGCGCGCGCCCCTCGGTGTATTATTGGCGTTTGGTGCCGGTGGGCTGCCCCAGAAAATAATGCATATTCCCCTTGTTTTTCCCAACCCCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATac                                                                                            <  3:465658/143‑1 (MQ=255)
                                                     ccATGCTCCTGCCCGCGGCCCTTTGCTTATTAATTGCGTTTAGTCCGGGTGGGCGGGCACCAAAAATAATGCATTTTCCCCTTGCTTTAACAAACCCCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATac                                                                                            <  1:383940/151‑1 (MQ=255)
                                                       ctGCTACTGCTCGCGGCACTCTGCTTTTTATTTGCGTTTAGTGCCTGTGGGCGGCCACCAAAAAAAATGCATATCGCCCGTGTTTTTCCCAACCCCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATacac                                                                                          <  3:557465/150‑1 (MQ=255)
                                                       aTGCTCCTGCCCGCGGCCCTCTGCTTATTAATTGCGTTTAGTGCATGTTGGCTGCCACGAAAGAAAATGCATATTCCCCTTGTTTTAACCAACCCCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATacac                                                                                          <  1:501751/151‑1 (MQ=255)
                                                       aTGCTCCTGCCCGCGGCCCTCTGCGTATTAATTGCGTTTAGTGCCAGTTGGCTGGCACCGAAAATAATGCATATTGCCCATGCTTTTACCAATCCCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATacac                                                                                          <  1:391208/151‑1 (MQ=255)
                                                       aTGCTCCTGCCCGCGGCACTCTGCTTATTAATTGCGTTTAGTGCCGGTTGGCGGGCACAGAAGATAATGCATTTTCCCCATGCTTTTCCCAACCCCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATacac                                                                                          <  2:1192005/151‑1 (MQ=255)
                                                       aTGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGGCTGGCACCGAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATaca                                                                                           >  3:473550/1‑150 (MQ=255)
                                                        tcctcctccccgcgACCCTCTGAGTATTAATTCCGAGTATTGCTTGTGGCCGCCACGCAAAAATAAGGATTATTCCCCTTGCTTTTACAAACCCCCCCCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATaca                                                                                           <  4:1312901/139‑1 (MQ=255)
                                                        tGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGGCTGGCACCGAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATacac                                                                                          >  4:708587/1‑150 (MQ=255)
                                                        tGCAACTGCTCGCGCCATTCTTCTTTTTATTTGCGTTTAGTGCTGTTTGGCGGCCCCGAAAGAAAAGTCTTATTGCGCATGCTTTAACACACCCCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATacac                                                                                          <  4:274137/150‑1 (MQ=255)
                                                        ggctccggCTCGCGGCCCTCTGCTTATTATTGGCGTTTAGTGCAGGTTGGCTGGCACCAAAGAAAATGCATATTGCCCAGGTTTATACAAACCCCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATacac                                                                                          <  1:64239/143‑1 (MQ=255)
                                                         gCTACTGCTCGCGGGACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGGCTGGCACCGAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATacac                                                                                          >  4:832666/1‑149 (MQ=255)
                                                          cTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGGCTGGCACCGAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAgg                                                                                       >  2:1346561/1‑151 (MQ=255)
                                                          cTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGGCTGGCACCGAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAgg                                                                                       >  1:338759/1‑151 (MQ=255)
                                                            ccggcccgcgGCTCTCTGTTTATTAATTGCTTTTAGTGCCGGTGGGCGGCCACAGAAAATAATCCATTTTCCCCTTGTTTTTCCCAATCCCCCCCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTc                                                                                     <  4:1121273/145‑1 (MQ=255)
                                                            ccTGCCCGCGCCATTCTTTTTATTATTTCCGTTTAGTGCAGTTTGGCGGCCACGAAAAAAAATGATTTTGCCCCATGTTTTTACCACCCCCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTc                                                                                     <  3:77891/150‑1 (MQ=255)
                                                               gcgcgcgGCTCTCTGTTTATTAATTGCGCTTGGTGCGGTTTGGCGGGCACCAAAAATATTGATTATCCCCCTTGCTTTTACCAATCCCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCa                                                                                    <  4:117817/146‑1 (MQ=255)
                                                                  cgcgGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGGCTGGCACCGAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCAccccc                                                                               >  4:1342178/1‑151 (MQ=255)
                                                                    ggcccctctGTGTATTATTGGCGTTTGGGCCGGTTGGGCGGCCCCCAAAGATAATATATTTTCCCCTTGCTTTTCCCAACCCCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTc                                                                             <  2:1027592/146‑1 (MQ=255)
                                                                       cattttgtttATTAATTGCGTTTAGTGCGGTTGGCCTGGCACCAAAAATAATCCATTTTCCCCTGGCGTTTCCAAACCCCCCCCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCAtt                                                                          <  4:25441/142‑1 (MQ=255)
                                                                        aCTCTGCGTATTAATTGCGCTTAGTGCCAGTTGGCTGGCACCGAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCAtt                                                                          >  2:649888/1‑150 (MQ=255)
                                                                         ctctGTGTATAATTTGCGTTGGGTCCTGGTGGGCGGGCACCAAAGATAATGCTTATTCCCCTTGCTTTTACCATCCCCCCCCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTAc                                                                        <  1:352929/151‑1 (MQ=255)
                                                                         ctctGCGTATTAATTGCGCTTAGTGCCAGTTGGCTGGCACCGAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTAc                                                                        >  4:1304031/1‑151 (MQ=255)
                                                                            tgtttataatttGCGTTTTGTGCGTGTGGGCTGCCACGAAAAATAATGTATATCCCCCTTGCTTTACCCTACCCCCCCCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGt                                                                     <  3:462129/141‑1 (MQ=255)
                                                                            tgtgTATTATTGGCTCTTGGTCCTTGTTGGCTGCCACCAAAAATAATGCATATTCCCCTTGTTTTTCCCAACCCCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGt                                                                     <  3:387918/148‑1 (MQ=255)
                                                                             gcttataagttGCGTTTCGTGCAAGTGGGCTGGCACAAAAAATAATGCATTTCCCCCTTGCTTTTACCACCCCCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACt                                                                       <  4:1370157/139‑1 (MQ=255)
                                                                             gCGTATTAATTGCGCTTAGTGCCAGTTGGCTGGCACCGAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGtt                                                                    >  3:348814/1‑151 (MQ=255)
                                                                             gCGTATTAATTGCGCTTAGTGCCAGTTGGCTGGCACCGAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGtt                                                                    >  1:333517/1‑151 (MQ=255)
                                                                             gCGTATTAATTGCGCTTAGTGCCAGTTGGCTGGCACCGAAGATAATGCATATTGCCCATGCGTCTACCAATACCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGtt                                                                    >  1:191775/1‑151 (MQ=255)
                                                                               gTATTAATTGCGCTTAGTGCCAGTTGGCTGGCACCGAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGTTGc                                                                  >  4:1022204/1‑151 (MQ=255)
                                                                                 aTTAATTGCGTTTAGTGCCGGTTGGCTGGCACCAAAGATAATGCATATTCCCCATGCTTTTACCAATCCCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGTTGCt                                                                 <  2:643087/150‑1 (MQ=255)
                                                                                 aTTAATTGCGCTTAGTGCCAGTTGGCTGGCACCGAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGTTGCTg                                                                >  2:252475/1‑151 (MQ=255)
                                                                                    aaTTGCGCTTAGTGCCAGTTGGCTGGCACCGAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGTTGCTGtt                                                              >  4:865897/1‑150 (MQ=255)
                                                                                    aaTTGCGCTTAGTGCCAGTTGGCTGGCACCGAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGTTGCTGtt                                                              >  2:411428/1‑150 (MQ=255)
                                                                                     aTTGCGCTTAGTGCCAGTTGGCTGGCACCGAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGTTGCTGTta                                                             >  3:146127/1‑150 (MQ=255)
                                                                                       tgcgttgggTGCCATGTGGCTGGCACCAAAGATAATGAATATTCCCCTTGCGTTTACCAACCCCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGTTGCTGTtacta                                                          <  1:2080/143‑1 (MQ=255)
                                                                                         cttttAGTGCCAGTTGGCTGGCCCCAAAAAAAATGCATATTGCCCATGCTTTTACCAATCCCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGTTGCTGTtactac                                                         <  1:563219/147‑1 (MQ=255)
                                                                                          gcTTAGTGCCAGTTGGCTGGCACCGAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGTTGCTGTTACTACTg                                                       >  4:608451/1‑151 (MQ=255)
                                                                                          cgtttggtccgggtgggctggcACCAAAGATAATGCATATTCCCCATGCTTTTACCAACCCCCCCCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGTTGCTGTTactact                                                        <  1:242474/136‑1 (MQ=255)
                                                                                           cTTAGTGCCAGTTGGCTGGCACCGAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGTTGCTGTTACTACTgg                                                      >  4:1390373/1‑151 (MQ=255)
                                                                                            ttAGTGCCAGTTGGCTGGCACCGAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGTTGCTGTTACTACTGGc                                                     >  2:1038789/1‑151 (MQ=255)
                                                                                              aGTGCCAGTTGGCTGGCACCGAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGTTGCTGTTACTACTGGCg                                                    >  2:1057028/1‑150 (MQ=255)
                                                                                                             gggcACCGAAAATAATGTATATTGCCCATGCTTTTACCACCCCCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGTTGCTGTTACTACTGGCGATGCCTTTGCTGcc                                      <  4:498428/150‑1 (MQ=255)
                                                                                                              ggcACCGAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGTTGCTGTTACTACTGGCGATGCCTTTGCTGCCt                                     >  4:917660/1‑151 (MQ=255)
                                                                                                              ggcACCGAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGTTGCTGTTACTACTGGCGATGCCTTTGCTGCCt                                     >  2:958998/1‑151 (MQ=255)
                                                                                                              ggcACCGAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGTTGCTGTTACTACTGGCGATGCCTTTGCTGCCt                                     >  2:911711/1‑151 (MQ=255)
                                                                                                               gcACCGAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGTTGCTGTTACTACTGGCGATGCCTTTGCTGCCTg                                    >  4:801461/1‑151 (MQ=255)
                                                                                                               gcACCGAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGTTGCTGTTACTACTGGCGATGCCTTTGCTGCCTg                                    >  2:286274/1‑151 (MQ=255)
                                                                                                                  ccGAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGTTGCTGTTACTACTGGCGATGCCTTTGCTGCCTGGcc                                 >  4:729392/1‑151 (MQ=255)
                                                                                                                  ccGAAGATAATGCATATTCCCCATGCTTTTCCCAATCCCCCCCCCGCCTCTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGTTGCTGTTACTACTGGCGATGCCTTTGCTGCCTGGcc                                 <  1:185965/151‑1 (MQ=255)
                                                                                                                  ccGAAAATAATGCATATTGCCCATGCTTTTCCAAACCCCCCCCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGTTGCTGTTACTACTGGCGATGCCTTTGCTGCCTGGcc                                 <  2:1359964/151‑1 (MQ=255)
                                                                                                                     gAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCTCCCGCCACTGTCGCCAGCGGAATAGCTCTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGTTGCTGTTACTACTGGCGATGCCTTTGCTGCCTGGCCtt                               >  4:32816/1‑151 (MQ=255)
                                                                                                                         aaTAATGCATTTCCCCCAGGCTTTTACCAATCCCCCCCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGTTGCTGTTACTACTGGCGATGCCTTTGCTGCCTGGCCTTTAc                            <  2:281629/150‑1 (MQ=255)
                                                                                                                           tAATGCATATTGCCCATGCGTTTACCAATACCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGTTGCTGTTACTACTGGCGATGCCTTTGCTGCCTGGCCTTTACTg                          >  4:1073422/1‑151 (MQ=255)
                                                                                                                             aTGCATATTGCCCATGCGTTTACCAATACCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGTTGCTGTTACTACTGGCGATGCCTTTGCTGCCTGGCCTTTACTGGc                        >  2:1107575/1‑151 (MQ=255)
                                                                                                                                gtattttccccATGCTTTTCCCAACCCCCCCCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGTTGCTGTTACTACTGGCGATGCCTTTGCTGCCTGGCCTTTACTGGCt                       <  2:111905/142‑1 (MQ=255)
                                                                                                                                    ttGCCCATGCGTTTACCAATACCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGTTGCTGTTACTACTGGCGATGCCTTTGCTGCCTGGCCTTTACTGGCTgtggtg                 >  1:561028/1‑151 (MQ=255)
                                                                                                                                    ttGCCCATGCGTTTACCAATACCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGTTGCTGTTACTACTGGCGATGCCTTTGCTGCCTGGCCTTTACTGGCTgtggtg                 >  3:373368/1‑151 (MQ=255)
                                                                                                                                     cgCCCCTGCGTTAACCACCCCCCCCCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGTTGCTGTTACTACTGGCGATGCCTTTGCTGCCTGGCCTTTACTGGCTGTGgtgt                <  3:332401/150‑1 (MQ=255)
                                                                                                                                       cccctgtGCTTTTACCCCTCCCCCCCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGTTGCTGTTACTACTGGCGATGCCTTTGCTGCCTGGCCTTTACTGGCTGTGgtgt                <  3:332393/145‑1 (MQ=255)
                                                                                                                                             tgtttttACAAACCCCCCCCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGTTGCTGTTACTACTGGCGATGCCTTTGCTGCCTGGCCTTTACTGGCTGTGGTGTCGTTc           <  4:88622/146‑1 (MQ=255)
                                                                                                                                             tGCGTTTACCAATACCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGTTGCTGTTACTACTGGCGATGCCTTTGCTGCCTGGCCTTTACTGGCTGTGGTGTCGTTcg          >  1:317624/1‑151 (MQ=255)
                                                                                                                                              gtttttACCAACCCCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGTTGCTGTTACTACTGGCGATGCCTTTGCTGCCTGGCCTTTACTGGCTGTGGTGTCGTTcg          <  2:806417/147‑1 (MQ=255)
                                                                                                                                              gCGTTTCCCACCCCCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGTTGCTGTTACTACTGGCGATGCCTTTGCTGCCTGGCCTTTACTGGCTGTGGTGTCGTTcg          <  4:1228055/150‑1 (MQ=255)
                                                                                                                                              gCGTTTACCAATACCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGTTGCTGTTACTACTGGCGATGCCTTTGCTGCCTGGCCTTACTGGCTGTGGTGTCGTTcgcg        >  1:513044/1‑151 (MQ=255)
                                                                                                                                                 tttACCAATACCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGTTGCTGTTACTACTGGCGATGCCTTTGCTGCCTGGCCTTTACTGGCTGTGGTGTCGTTcgcgc       >  1:542725/1‑150 (MQ=255)
                                                                                                                                                  ttACCAATACCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGTTGCTGTTACTACTGGCGATGCCTTTGCTGCCTGGCCTTTACTGGCTGTGGTGTCGTTcgcgccg     >  4:1296390/1‑151 (MQ=255)
                                                                                                                                                       aaTACCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGTTGCTGTTACTACTGGCGATGCCTTTGCTGCCTGGCCTTTACTGGCTGTGGTGTCGTTcgcgccgcgc  >  2:1360048/1‑149 (MQ=255)
                                                                                                                                                          |                                                                                                                                                 
GCGCAGTACACACGCTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGGCTGGCACCGAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGTTGCTGTTACTACTGGCGATGCCTTTGCTGCCTGGCCTTTACTGGCTGTGGTGTCGTTCGCGCCGCGC  >  NC_000913/2603194‑2603487

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: