Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,332,7910AC42.0% 217.6 / 24.0 112V161G (GTG→GGG) basSsensory histidine kinase in two‑component regulatory system with BasR
Reads supporting (aligned to +/- strand):  ref base A (55/10);  new base C (0/47);  total (55/57)
Fisher's exact test for biased strand distribution p-value = 4.80e-22
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 112 total aligned reads displayed.

ATGTTGATAATTACCGGAAGAAAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTG‑CTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATG  >  NC_000913/4332643‑4332937
                                                                                                                                                     |                                                                                                                                                  
aTGTTGATAATTACCGGAAGAAAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTG‑CTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCAcc                                                                                                                                                  <  1:542070/151‑1 (MQ=255)
    tGATAATTACCGGAAGAAAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTG‑CTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCAccccc                                                                                                                                               <  2:1347307/150‑1 (MQ=255)
    tGATAATTACCGGAAGAAAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTG‑CTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCg                                                                                                                                              >  2:723116/1‑151 (MQ=255)
     gATAATTACCGGAAGAAAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTG‑CTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCg                                                                                                                                              <  4:1397637/150‑1 (MQ=255)
     gATAATTACCGGAAGAAAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTG‑CTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCg                                                                                                                                              >  3:260718/1‑150 (MQ=255)
     gATAATTACCGGAAGAAAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTG‑CTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGc                                                                                                                                             <  2:1307382/151‑1 (MQ=255)
     gATAATTACCGGAAGAAAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTG‑CTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGc                                                                                                                                             >  4:247696/1‑151 (MQ=255)
      aTAATTACCGGAAGAAAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTG‑CTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGcc                                                                                                                                            >  2:577049/1‑151 (MQ=255)
       tAATTACCGGAAGAAAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTG‑CTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGcc                                                                                                                                            >  2:807437/1‑150 (MQ=255)
        aaTTACCGGAAGAAAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTG‑CTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGcc                                                                                                                                            >  4:1251911/1‑149 (MQ=255)
         aTTACCGGAAGAAAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTG‑CTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGt                                                                                                                                         >  2:321389/1‑151 (MQ=255)
            aCCGGAAGAAAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTG‑CTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGc                                                                                                                                      >  2:849704/1‑151 (MQ=255)
             ccGGAAGAAAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTG‑CTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCTGTGGCg                                                                                                                                     >  4:676387/1‑151 (MQ=255)
             ccGGAAGAAAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTG‑CTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCg                                                                                                                                     >  2:332318/1‑151 (MQ=255)
                  aaaaaagggctccccGGCGGGCGGCAGCTGCAGCAGGGGGGGAACGCCCCCCATCATGTGATCAATCCGCCCCACTAGCGGCG‑CAACAAAAAGGTATTGCGTTTTCCCCATCAGTGCGAGATGCAAGCGCACCCCCGCCAGTGGCGTTCGc                                                                                                                                <  3:482757/138‑1 (MQ=255)
                      aaTGCCTGTCCGGCCCGCGCCGTTTGCAGCAGCGGGGAGCCCCTCTCCACCACCTAATAAAGCCGTCCCACTAACGTTG‑CTACAAAAATGTGATGTGTTTTCGCCAGCATTTCCAGAGACAAACGCCCCCCCGCCAGTGGCGTTCGCAGtt                                                                                                                            <  4:1333057/151‑1 (MQ=255)
                      aaTGCCTGCCCGCCCCGCCCCGGTTGCAGCAGCGGGGAGACGCTCTCCTTCATCTGATCAAGCCGTGCCACTAACGGTG‑CTACATCAATGTGATGCGTTTCCGCCAGCAGTCCCAGATGAAAACCCCCCCCCGCCAGTGGCGTTCGCAGtt                                                                                                                            <  3:529448/151‑1 (MQ=255)
                      aaTGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTG‑CTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGtt                                                                                                                            >  2:661370/1‑151 (MQ=255)
                          tcTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTG‑CTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTg                                                                                                                        >  1:247924/2‑151 (MQ=255)
                          aCTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTG‑CTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTg                                                                                                                        >  2:1168954/1‑151 (MQ=255)
                            tgcccGGCCCGCGCCAGTTGCAGCAGCGGGGAGCCCCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTG‑CTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTgcg                                                                                                                      <  3:91672/148‑1 (MQ=255)
                            tgcccGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTG‑CTACATCAATGTGATGCGTTTTCGCCAGCATTTCCAGATGCAACCGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTgcg                                                                                                                      <  1:413811/148‑1 (MQ=255)
                             gcccGGCCCGCGCCAGTTGCAGCGGGTGGGAGCCCCTCTCCATCATCTGACAAAGCCGTGCCACTACCGGTG‑CTACATCAATGTGATGCTTTTTCGCCAGCAGTTCAAGAGGCAAACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTgcgc                                                                                                                     <  4:1221656/149‑1 (MQ=255)
                               ccGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTG‑CTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGcgcg                                                                                                                    >  4:648706/1‑150 (MQ=255)
                                  gcccgcgCCAGTTGCAGCGGCGGGGAGACGCTCTCCATCATCTGATCAGGCCGTGCCACTAACGGTG‑CTACATAAATGTGATGCGTTTTCGCCAGAAGTTCCAGATGAAACCGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGt                                                                                                                <  2:232274/148‑1 (MQ=255)
                                   cctctcccgttcccggcagcTGGGAGCCCCTCCCCATCATCTAACAAAGCCGGGCCACTACCGGTG‑CTACATCATTGTGATGCGTTTCCGCCGGCGGTTCCAGATGCAAACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTc                                                                                                               <  4:1261387/136‑1 (MQ=255)
                                   cACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTG‑CTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTc                                                                                                               >  2:850435/1‑151 (MQ=255)
                                      gcgcCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTG‑CTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGc                                                                                                            >  2:122764/1‑151 (MQ=255)
                                      gcgcCAGTTGCAGCAGCGGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTG‑CTACATCAATGTGATGCGTTTTCGCCAGCATTTCCAGATGCAAACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGc                                                                                                            <  2:1216375/151‑1 (MQ=255)
                                       cgcCATTTGCAGCGGGGGGGAGCCCCTCCCCATCATCTGATCAAGCCGGCCCACTAACGGTG‑CTACATAAAGGGGATGCGTTTCCGCCAGCATTTCAAGATGCAACCGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCg                                                                                                           <  4:1343030/151‑1 (MQ=255)
                                       cgcCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTG‑CTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCg                                                                                                           >  4:961436/1‑151 (MQ=255)
                                           aGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTG‑CTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTa                                                                                                        >  2:585275/1‑150 (MQ=255)
                                           aGTTGCAGCAGCGGGGAGACGCTCCCCATCATCTGATCAAGCCGTGCCACTAACGGTG‑CTACATCAATGTGATGCTTTTGCGCCAGCATTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTaa                                                                                                       <  2:552711/151‑1 (MQ=255)
                                            gTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTG‑CTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTaa                                                                                                       >  2:1138958/1‑150 (MQ=255)
                                             ttGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTG‑CTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAaac                                                                                                     >  2:1448205/1‑151 (MQ=255)
                                              tGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTG‑CTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAaac                                                                                                     >  4:1422633/1‑150 (MQ=255)
                                               gcagcgGCTGGGAGCCGCTCCCCATCATCTGATCAAGCCGTGCCACAAACGGTG‑CTACATAAATGGGATGCTTTTCCGCCAGCAGTTCCAGATGCAACCGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAaaca                                                                                                    <  4:574520/150‑1 (MQ=255)
                                               gcagcgGCGGGGAGACGCTCTCCATCATCTGATAAAGCCGTCCCACTAACGGTG‑CTACATCAGTGTGATGCGTTTTCGCCAGCAGTCCCAGATGCAAACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAaaca                                                                                                    <  4:1399978/150‑1 (MQ=255)
                                                cagcagCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTG‑CTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTc                                                                                                               >  2:1261387/1‑138 (MQ=255)
                                                 agcagcTGGGAGCCGCTCCCCATCATCTGATCAAGCCGTGCCACTAACGGTG‑CTACATCAATGTGATGCGTTTCCGCCAGCATTCCCAGATGAAACCGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAAcc                                                                                                 <  2:313641/151‑1 (MQ=255)
                                                     gcTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTG‑CTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCttt                                                                                              >  3:10631/1‑150 (MQ=255)
                                                       tGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTG‑CTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGt                                                                                           >  4:790462/1‑151 (MQ=255)
                                                        gggAGCCGCTCTCCATCATCTGATCAGGCCGTGCCACAACCGGTG‑CTACATCAATGTGATGCGTTTTCGCCAGCAGTCCCAGATGCAAACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGtt                                                                                          <  2:361844/151‑1 (MQ=255)
                                                          gggcccttttcCACCATTTGACAAACCCGCGCCAAAAGCGGTG‑CTACATAAAGGTGATGGTTTTGGGCCAGGAGTTCCAGAGGAAACCGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTAt                                                                                        <  4:1049955/142‑1 (MQ=255)
                                                             cccctccCCTTCTTCTGATCAGGCCGTGCCACGAGCGGTG‑CTACATCAATGTGATGCGTTCTCCCAACCCTTTCCAGAGCAAAACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATcc                                                                                      <  3:120655/147‑1 (MQ=255)
                                                             accctctCCATCATCTGACAAAGCCGTGCCACTACGGGTG‑CTACATAAATGTGAGGCTTTTCCGCCAGAATTTCCAGAGCCAAACCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATcc                                                                                      <  1:384893/147‑1 (MQ=255)
                                                             accccctcCATCATCTAACAAACCGGTCCAACAAACGGTG‑CTACAACAATGGGATGCGTTTCCGCCAGAAGTCCCAGATAAAACCCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATcc                                                                                      <  3:399533/145‑1 (MQ=255)
                                                              cGCTCTCCATCATCTGATCAAGCCGTGCCACTAAGGGTG‑CTACACCAATGTGATGCTTTTTCGCCAGCAGTTCCAGATGAAAACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAg                                                                                    <  3:492628/151‑1 (MQ=255)
                                                                  ctcCATCATCTGATCAAGCCGTGCCACTAACGGTG‑CTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTg                                                                                >  2:266344/1‑151 (MQ=255)
                                                                  ctcCATCATCTGATCAAGCCGTGCCACTAACGGTG‑CTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTg                                                                                >  3:346466/1‑151 (MQ=255)
                                                                  ccccaccaTCTAACAAAGCCGTGCCACTACCGGTG‑CTACATAAATGTGTTTTTTTTTCGCCAGCTGTCCCAGATCCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCg                                                                                  <  4:1142884/147‑1 (MQ=255)
                                                                       tcattTGATCAAGCCGTGCCACTACCGGTG‑CAACATCATTGTGATGCTTTTCCCCAACCATTTCCAGATGCAACCCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTg                                                                             <  2:851213/149‑1 (MQ=255)
                                                                        catcGGATCAGCCCGTCCCACTACCGGTT‑CTACATCATTGTGATGCTTTTTCGCCAGCAGTTCCAGATGAAAACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTc                                                                          <  2:255772/151‑1 (MQ=255)
                                                                        catcGGATCAAGCCGTGCCACTAACGGTC‑CACAATCAAGGTGATGCTTTTCCGCCAGCAGTTCCAAATGAAACCGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTc                                                                          <  1:37919/151‑1 (MQ=255)
                                                                          tcggaccaggcggTGCCACTAACGGTG‑CTACAACAATGTGATGTGTTTTCCCCAGCAGTTCAAGGGGCAACCCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCa                                                                         <  4:381647/138‑1 (MQ=255)
                                                                            tGATCAAGCCGTGCCACTAACGGTG‑CTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGc                                                                      >  4:1025744/1‑151 (MQ=255)
                                                                            tGATCAAGCCGTGCCACTAACGGTG‑CTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGc                                                                      >  2:396611/1‑151 (MQ=255)
                                                                              accAAGCCGTGCCACTACCGGTG‑CTACATCAATGTGATGCTTTTTCGCCAGCAGTCCCAGATGCAAACCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCg                                                                     <  3:262126/148‑1 (MQ=255)
                                                                                 aaGCCGGGCCAC‑AACGGGGCCTACATAAATGGGAGGCGTTTTCGCCAGCGGTGCCAGATGAAAACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTg                                                                 <  2:1423709/151‑1 (MQ=255)
                                                                                     ngTGCCACTAACGGTG‑CTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGAcc                                                              >  4:529027/2‑150 (MQ=255)
                                                                                     cGTGCCATTAAGGTTG‑CTACAAAATGGTGATGCTTTTTCGCAAGCTTTTCCAGATGCAAACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCa                                                             <  4:1126654/151‑1 (MQ=255)
                                                                                      gTGCCACTAACGGTG‑CTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCATCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAg                                                            >  1:192546/1‑151 (MQ=255)
                                                                                      gTGCCACTAACGGTG‑CTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGAcc                                                              >  2:312543/1‑149 (MQ=255)
                                                                                      gTGCCACTAACGGTG‑CTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGAcc                                                              >  4:648105/1‑149 (MQ=255)
                                                                                              accGGTG‑CTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAACCGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGtt                                                      <  2:805177/147‑1 (MQ=255)
                                                                                              aaggggg‑CTCCATAAATGTGTTGTTTTTCCCCCAGCATTTCCAGAGGAAACCGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGtt                                                      <  4:1322088/143‑1 (MQ=255)
                                                                                              aaCGTTG‑CTACACAAATTTGATGCGTTTTCGCCAGCATTTCCAAAGGCAAGCGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGtt                                                      <  2:612915/149‑1 (MQ=255)
                                                                                              aaCGGTG‑CTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGttt                                                     >  4:183917/1‑150 (MQ=255)
                                                                                              aaCGGTG‑CTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGttt                                                     >  2:1209004/1‑150 (MQ=255)
                                                                                                gggTG‑CTACATAAATGTGATGTGTTTCCGCCAGCCGTCCCAGATGCAAACCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAAc                                                  <  2:514418/150‑1 (MQ=255)
                                                                                                  gTG‑CTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACg                                                 >  1:394556/1‑150 (MQ=255)
                                                                                                  gTG‑CTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGGTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGc                                                >  4:154161/1‑151 (MQ=255)
                                                                                                   tG‑CTACATCAATGTGATGCTTTTTCGCCAGCAGTTCCAGATGCAACCGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGc                                                <  1:378462/150‑1 (MQ=255)
                                                                                                   tG‑CTACATCAATGTGATGCTTTTCCGCCAGCATTCCCAGATGCAAACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGc                                                <  1:299264/150‑1 (MQ=255)
                                                                                                   tG‑CTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGcc                                               >  4:105076/1‑151 (MQ=255)
                                                                                                   tG‑CTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGc                                                >  1:403818/1‑150 (MQ=255)
                                                                                                       caCATAATTGTGATGCTTTTCCGCCAGCGGTCCCAGATGCAACCGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGaa                                            <  2:77578/150‑1 (MQ=255)
                                                                                                        aCATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGaaa                                           >  1:312453/1‑151 (MQ=255)
                                                                                                        aCATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGaaa                                           >  3:324255/1‑151 (MQ=255)
                                                                                                        aCATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGaaa                                           >  3:463339/1‑151 (MQ=255)
                                                                                                         cATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGaaa                                           >  3:262991/1‑150 (MQ=255)
                                                                                                          aTCAATGTGTGGCGTTTCCGCCAGCAGTTCCAGATGCAAACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAacc                                         <  3:93764/151‑1 (MQ=255)
                                                                                                            cAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAACCGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAacca                                        <  2:148488/150‑1 (MQ=255)
                                                                                                             aaTGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAaccacc                                      >  4:928651/1‑151 (MQ=255)
                                                                                                                    ggCGTTTTCGCCAGGCTTCCCAGGTGCAAACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGa                               <  2:247692/150‑1 (MQ=255)
                                                                                                                           ccGCAAGCAGTCCCAGATGCAAACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAg                        <  4:661367/150‑1 (MQ=255)
                                                                                                                             cccAGCAGTTCCAGATGAAAACCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAggg                      <  2:676390/150‑1 (MQ=255)
                                                                                                                              ccAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGt                     >  2:1293594/1‑151 (MQ=255)
                                                                                                                                agctgTGCCAGAGCCAAACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTgg                   <  4:1411128/151‑1 (MQ=255)
                                                                                                                                agcagTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTgg                   >  2:1331069/1‑151 (MQ=255)
                                                                                                                                   attcccAGATGCAACCCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGgcgc                <  3:270175/147‑1 (MQ=255)
                                                                                                                                    gtcccAGAGGCAAACCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCt               <  4:723113/148‑1 (MQ=255)
                                                                                                                                    gTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGgcg                 >  3:480540/1‑149 (MQ=255)
                                                                                                                                      tCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCtgt             >  2:786856/1‑151 (MQ=255)
                                                                                                                                       ccAGATGCAAACCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCtgtg            <  4:1439846/151‑1 (MQ=255)
                                                                                                                                         aGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGa           >  4:383498/1‑150 (MQ=255)
                                                                                                                                           aggCAACCGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATg        <  4:410841/149‑1 (MQ=255)
                                                                                                                                               aaaCGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCa     >  4:1254564/1‑150 (MQ=255)
                                                                                                                                                 aCGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATg  <  4:332323/151‑1 (MQ=255)
                                                                                                                                                 aCGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATg  >  1:185642/1‑151 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
ATGTTGATAATTACCGGAAGAAAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTG‑CTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATG  >  NC_000913/4332643‑4332937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑