Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,538,1090AC43.7% 201.3 / 36.7 119G219G (GGT→GGG) nanMN‑acetylneuraminic acid mutarotase
Reads supporting (aligned to +/- strand):  ref base A (50/17);  new base C (0/52);  total (50/69)
Fisher's exact test for biased strand distribution p-value = 4.37e-19
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 120 total aligned reads displayed.

TCCCCGCAAAACCGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAA‑TTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAAT‑CCTGGTTTGGCTTCGCC‑ATTAATAAGCCAGGTTTT‑ATCACC‑TTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTT  >  NC_000913/4537961‑4538258
                                                                                                                                                        |                                                                                                                                                       
 ccccGCAAAACCGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAA‑TTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAAT‑CCTGGTTTGGCTTCGCC‑ATTAATAAGCCAGGTTTT‑ATCAcc                                                                                                                                                       <  4:292716/150‑1 (MQ=255)
   ccGCAAAACCGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAA‑TTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAAT‑CCTGGTTTGGCTTCGCC‑ATTAATAAGCCAGGTTTT‑ATCACC‑ttt                                                                                                                                                   >  3:149612/1‑151 (MQ=255)
   ccGCAAAACCGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAA‑TTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAAT‑CCTGGTTTGGCTTCGCC‑ATTAATAAGCCAGGTTTT‑ATCACC‑ttn                                                                                                                                                   <  4:1311437/151‑2 (MQ=255)
     gCAAAACCGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAA‑TTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAAT‑CCTGGTTTGGCTTCGCC‑ATTAATAAGCCAGGTTTT‑ATCACC‑TTT‑At                                                                                                                                                <  4:1259557/151‑1 (MQ=255)
      cAAAACCGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAA‑TTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAAT‑CCTGGTTTGGCTTCGCC‑ATTAATAAGCCAGGTTTT‑ATCACC‑TTT‑Att                                                                                                                                               <  2:110191/151‑1 (MQ=255)
       aaaaCCGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAA‑TTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAAT‑CCTGGTTTGGCTTCGCC‑ATTAATAAGCCAGGTTTT‑ATCACC‑TTT‑ATTc                                                                                                                                              <  3:199977/151‑1 (MQ=255)
       aaaaCCGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAA‑TTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAAT‑CCTGGTTTGGCTTCGCC‑ATTAATAAGCCAGGTTTT‑ATCACC‑TTT‑ATTc                                                                                                                                              <  3:207313/151‑1 (MQ=255)
       aaaaCCGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAA‑TTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAAT‑CCTGGTTTGGCTTCGCC‑ATTAATAAGCCAGGTTTT‑ATCACC‑TTT‑ATTc                                                                                                                                              <  4:1059761/151‑1 (MQ=255)
        aaaCCGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAA‑TTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAAT‑CCTGGTTTGGCTTCGCC‑ATTAATAAGCCAGGTTTT‑ATCACC‑TTT‑ATTca                                                                                                                                             <  2:308718/151‑1 (MQ=255)
           ccGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAA‑TTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAAT‑CCTGGTTTGGCTTCGCC‑ATTAATAAGCCAGGTTTT‑ATCACC‑TTT‑ATTCACaa                                                                                                                                          <  4:845815/151‑1 (MQ=255)
            cGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAA‑TTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAAT‑CCTGGTTTGGCTTCGCC‑ATTAATAAGCCAGGTTTT‑ATCACC‑TTT‑ATTCACAAc                                                                                                                                         <  4:1225237/151‑1 (MQ=255)
            cGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAA‑TTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAAT‑CCTGGTTTGGCTTCGCC‑ATTAATAAGCCAGGTTTT‑ATCACC‑TTT‑ATTCACAAc                                                                                                                                         <  1:199748/151‑1 (MQ=255)
                  cTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAA‑TTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAAT‑CCTGGTTTGGCTTCGCC‑ATTAATAAGCCAGGTTTT‑ATCACC‑TTT‑ATTCACAAccgccg                                                                                                                                    >  3:188428/1‑150 (MQ=255)
                      gCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCTTTTTAAA‑TTATTACCGGTGAAATCAATTTCAAATACGGCATCCGTTCGCAAT‑CCTGTTTTGGCTTCGCC‑ATTAATAAGCCAGGTTTT‑ATCCCC‑TTT‑ATTCACAACCGCCGCACCa                                                                                                                               <  4:4760/151‑1 (MQ=255)
                       ccATCTGGTGATGAGACGGGACCAAGTTTATTCCATTTTAAA‑TTATTACCGGTGAAATCAAGTTAAAATACGGCATCCGTTCGCAAT‑CCTGGTTTGGCTTCGCC‑ATTAATAAGCCAGTTTTT‑ATCCCT‑TTT‑ATTCACAACCGCCGCACCAg                                                                                                                              <  4:1468652/151‑1 (MQ=255)
                         aTCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAA‑TTATTACCGGTAAAACCAAGTTAAAATACGGCATCCTTCCGCAAT‑CCTGTTTTGGCTTCGCC‑ATTAATAAGCCAGTTTTT‑ATCACC‑TTT‑ATTCACAACCGCCGCACCAGcc                                                                                                                            <  3:48455/151‑1 (MQ=255)
                             ggTGATGAGACGGGAGCAAGCTTATTCCATTTTAAA‑TTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAAT‑CCTGGTTTGGCTTCGCC‑ATTAATAAGCCAGGTTTT‑ATCACC‑TTT‑ATTCACAACCGCCGCACCAGCCGtt                                                                                                                         >  1:141076/1‑150 (MQ=255)
                                gatgaGACGGGAGCAAGCTTATTCCATTTTAAA‑TTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAAT‑CCTGGTTTGGCTTCGCC‑ATTAATAAGCCAGGTTTT‑ATCACC‑TTT‑ATTCACAACCGCCGCACCAGCCGTTCCGt                                                                                                                     >  1:369605/1‑151 (MQ=255)
                                  tgaGACGGGAGCAAGCTTATTCCATTTTAAA‑TTATTCCCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAAT‑CCTGGTTTGGCTTCGCC‑ATTAATAAGCCAGGTTTT‑ATCCCC‑TTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTAc                                                                                                                   <  1:111778/151‑1 (MQ=255)
                                   gagcCGGGAGCAAGCTTTTTCCATTTAAAA‑TTATTCCGGGTAAAATAAAGTTCAAATCCGGCTTCCGTTCGCAAT‑CCTGTTTTGCCTTCGCC‑ATTAATAAGCCAGTTTTT‑ATCCCC‑TTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTAc                                                                                                                   <  4:852631/150‑1 (MQ=255)
                                   gagaCGGGAGCAAGCTTATTCCATTTTAAA‑TTATTACCGGTGAAATCAAGTCCAAATACGGCATCCGTTCGCAAT‑CCTGGTTTGGCTTCGCC‑ATTAATAAGCCAGGTTTT‑ATCACC‑TTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTAcc                                                                                                                  >  4:1290698/1‑151 (MQ=255)
                                    agaCGGGAGCAAGCTTATTCCATTTTAAA‑TTATTCCGGGTAAAATCAAGTTAAAATCCGGCATCCTTTCGCAAC‑CCTGTTTTGCCTCCCCC‑ATAAATAAGCCAGTTTTT‑ATCCCT‑TTT‑ATCCACAACCGCCGCACCAGCCGTTCCGTAc                                                                                                                   <  3:21366/149‑1 (MQ=255)
                                          gAGCAAGCTTATTCCATTTTAAA‑TTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAAT‑CCTGGTTTGGCTTCGCC‑ATTAATAAGCCAGGTTTT‑ATCACC‑TTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCg                                                                                                            >  4:731734/1‑150 (MQ=255)
                                           aGCAAGCTTATTCCATTTTAAA‑TTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAAT‑CCTGGTTTGGCTTCGCC‑ATTAATAAGCCAGGTTTT‑ATCACC‑TTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGAt                                                                                                          >  4:911462/1‑151 (MQ=255)
                                           aGCAAGCTTATTCCATTTTAAA‑TTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAAT‑CCTGGTTTGGCTTCGCC‑ATTAATAAGCCAGGTTTT‑ATCACC‑TTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGAt                                                                                                          >  4:842710/1‑151 (MQ=255)
                                            gCAAGCTTTTTCCATTTTAAA‑TTATTCCCGGGAAAATCAAGTTAAAATACGGCATCCGTTCGCAAT‑CCTGTTTTGGCTTCGCC‑ATTAAAAAGCCAGGTTTT‑ATCCCC‑TTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGAtt                                                                                                         <  2:1063045/151‑1 (MQ=255)
                                            acaacttttttCCTTTTTAAA‑TTTTTCCCGGTAAAATCAATTTAAAATCCGCCCCCCGTTCCAAAC‑CCGGGTTTGGCTTCCCC‑ATAAAAAAGCCAGTTTTT‑ATCCCT‑TTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGAtt                                                                                                         <  4:698728/142‑1 (MQ=255)
                                             cAAGCTTATTCCATTTTAAA‑TTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAAT‑CCTGGTTTGGCTTCGCC‑ATTAATAAGCCAGGTTTT‑ATCACC‑TTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTc                                                                                                        >  2:1030066/1‑151 (MQ=255)
                                               cgCTTCTTCCTTTTTCCC‑TTCTTACCGGTGCCCTCCCGTTCAAATACGGCATCCGTTCGCCCT‑CCTGGTTTGGCTTCGCC‑ATTAATAAGCCAGGTTTT‑ATCACC‑TTT‑ATTCACAACCGCCGCCCCAGCCGTTCCGTACCAGGGCGATTCg                                                                                                       >  1:396431/2‑150 (MQ=255)
                                                gttttttCCTTTTTAAT‑TTATTACCGGGAAAACAAATTTAAAATCCGGCTCCCGTCCGCATC‑CGTGGTTTGCCTCCCCT‑ATAAAAACGCCGGTTTTT‑ACCCCC‑TTT‑TCCCCCACCCCCCGCCCCAGCCGTTCCGTACCAGGGCGATTCGcc                                                                                                     <  4:1432378/146‑1 (MQ=255)
                                                gCTTATTCCATTTTAAA‑TTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAAT‑CCTGGTTTGGCTTCGCC‑ATTAATAAGCCAGGTTTT‑ATCACC‑TTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGc                                                                                                      >  1:429692/1‑150 (MQ=255)
                                                 ttttttCCTTTTTAAT‑TTATTCCCGGTAAAATCAATTTAAAATACGCCCTCCTTTCGCAAT‑CTTGGTTTGCCTTCCCC‑ATAAATAACCCGGTTTTT‑ATCCCT‑TTT‑ATCCACACCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGcc                                                                                                     <  4:262376/146‑1 (MQ=255)
                                                  ttATTCCATTTTAAA‑TTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAAT‑CCTGGTTTGGCTTCGCC‑ATTAATAAGCCAGGTTTT‑ATCACC‑TTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGcc                                                                                                     >  2:1155142/1‑149 (MQ=255)
                                                     tttcATTTTAAATTTATTCCCGGTAAAATAAATTTAAAATCCGGC‑TTCGTTCGCCATCCCTGTTTTGTTTCCGCC‑TTAAATAAGCC‑TGTTTT‑TTCCCC‑TTTAATCCACACCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGc                                                                                                  <  4:242535/147‑1 (MQ=255)
                                                      tCCTTTTAAAT‑TTATTCCGGGTAAAATAAATTTAAAATCGGGCATCCTTCCGCATT‑CTTGGTTTGTTTTCCCA‑ATTAAAAAGCC‑GGTTTT‑TTCCCCTTTT‑ATTCAAAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGt                                                                                                <  4:1201607/150‑1 (MQ=255)
                                                      tCCATTTTAAA‑TTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAAT‑CCTGGTTTGGCTTCGCC‑ATTAATAAGCCAGGTTTT‑ATCACC‑TTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGt                                                                                                >  4:1400069/1‑150 (MQ=255)
                                                      cccATTTTAAA‑TTATGCCCGGTAAAACCAATTTAAAAAACGGCATCCTTTCGCAAT‑CCTGTTTGGGCTCCGCC‑A‑TAAAGAGCCCGGTTTTAACCACC‑TTT‑ATTCCAACCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTa                                                                                               <  4:41206/150‑1 (MQ=255)
                                                         aTTTTAAA‑TTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAAT‑CCTGGTTTGGCTTCGCC‑ATTAATAAGCCAGGTTTT‑ATCACC‑TTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTaa                                                                                              >  3:214412/1‑149 (MQ=255)
                                                         aTTTTAAA‑TTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAAT‑CCTGGTTTGGCTTCGCC‑ATTAATAAGCCAGGTTTT‑ATCACC‑TTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTaa                                                                                              >  3:327220/1‑149 (MQ=255)
                                                          ttttAAT‑TTATTCCGGGTGAAATCAGGTAAAAATAGGGCACCCATTCACAAC‑CCTGGTTTGGCTCCCCA‑ATTAATAAGCCGGTTTTT‑ATCCCC‑TTT‑ATCCAAAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTc                                                                                           <  1:455847/151‑1 (MQ=255)
                                                          tttaAAA‑TTATTCCCGGGAAAACAAATTTAAAATACGGCACCCGTCCGCAAT‑CCTGTTTGGGCTCCCCC‑ATTAATAGGCCAGTTTTT‑ACCCCC‑TTT‑ATCCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTc                                                                                           <  3:102454/151‑1 (MQ=255)
                                                              aaa‑TTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAAT‑CCTGGTTTGGCTTCGCC‑ATTAATAAGCCAGGTTTT‑ATCACC‑TTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCAtt                                                                                       >  2:764039/1‑151 (MQ=255)
                                                                a‑ttattCCCGGTAAAATCAATTTAAAATACGGCATCCGTCCGCAAT‑CCTGTTTTGGCTTCGCC‑ATTAATAAGCCAGTTTTT‑ACCCCT‑TTT‑TTTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGc                                                                                     <  1:188430/151‑1 (MQ=255)
                                                                a‑ttattACCGGTGAAATAAAGTTCAAATACGGCACCCGTTCGCAAT‑CCTGGTTTGGCTTCGCC‑ATTAATAAGCCAGGTTTT‑ATCACC‑TTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGc                                                                                     <  1:444042/151‑1 (MQ=255)
                                                                  tttttcccGGTAAAATAAAGTTAAAATACGCCTTCCGTCCCCAAT‑CCGGTTTGGGTTTCCCC‑TTTAATAGGCCAGTTTTT‑ATCCCC‑TTT‑ATCCCCAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGc                                                                                     <  4:101830/144‑1 (MQ=255)
                                                                   tattcCCGGTAAAATCAAGTTCAAATACGGCATCCGTTCGCAAT‑CCTGGTTTGGCTTCGCC‑ATTAATAAGCCAGGTTTT‑ATCCCC‑TTT‑ATCCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCtg                                                                                   <  1:557163/151‑1 (MQ=255)
                                                                    attcCCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAAT‑CCTGTTTTGGCTTCCCC‑ATTAATAAGCCAGGTTTT‑ATCCCC‑TTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCtgt                                                                                  <  2:601004/151‑1 (MQ=255)
                                                                     ttaCCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAAT‑CCTGGTTTGGCTTCGCC‑ATTAATAAGCCAGGTTTT‑ATCACC‑TTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCtgtg                                                                                 >  4:183225/1‑151 (MQ=255)
                                                                      tcccGGTAAAATAAAGTTAAAATCCGGCTTCCTTCCCCAAT‑CTTGTTTTGCCTCCCCC‑ATAAAAAAGCCATGTTTT‑ACCCCT‑TTT‑ATCCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTgtgt                                                                                <  3:459778/149‑1 (MQ=255)
                                                                       aCCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAAT‑CCTGGTTTGGCTTCGCC‑ATTAATAAGCCAGGTTTT‑ATCACC‑TTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGtt                                                                               >  2:738424/1‑151 (MQ=255)
                                                                        cgggTAAAATAAAGTTAAAATCCGGCATCCGTTCGCAAT‑CCTGGTTTGGCTTCGCC‑ATTAATAACCCAGTTTTT‑ATCCCC‑TTT‑ATTCACACCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTg                                                                              <  3:328979/149‑1 (MQ=255)
                                                                        ccGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAAT‑CCTGGTTTGGCTTCGCC‑ATTAATAAGCCAGGTTTT‑ATCACC‑TTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTg                                                                              >  2:469163/1‑151 (MQ=255)
                                                                          ggTGAAATCAAGTTCAAATACGGCATCCGTTCGCAAT‑CCTGGTTTGGCTTCGCC‑ATTAATAAGCCAGGTTTT‑ATCACC‑TTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAg                                                                            >  3:92511/1‑151 (MQ=255)
                                                                             aaaaaCAATTTAAAATACGCCACCCTTTCCCACC‑CCTGGTTGGGCTTCGCC‑ATAAATAACCCAGGTTTT‑ATCCCC‑TTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAggg                                                                          <  4:45318/149‑1 (MQ=255)
                                                                                  cAATTTAAAATCCGGCACCCGTTCGCAAT‑CCTGTTTTGGCTTCGCC‑ATTAATAAGCCAGTTTTT‑ACCCCC‑TTT‑ATCCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCaa                                                                     <  4:46028/150‑1 (MQ=255)
                                                                                  cAAGTTCAAATACGGCATCCGTTCGCAAT‑CCTGGTTTGGCTTCGCC‑ATTAATAAGCCAGGTTTT‑ATCACC‑TTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCaa                                                                     >  4:219701/1‑150 (MQ=255)
                                                                                  aaaGTTAAAATCCGGCATCCGTTCGCAAT‑CCTGTTTTGGCTTCGCC‑ATTAATAACCCAGTTTTT‑ATCCCC‑TTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCaaa                                                                    <  4:187670/150‑1 (MQ=255)
                                                                                      ttaaaagccGGCACCCGTTCGCATT‑CCTGTTTTGGCTTCGCC‑ATTAATAAGCCAGTTTTT‑ATCCCT‑TTT‑ATCCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGa                                                                 <  4:1391390/142‑1 (MQ=255)
                                                                                       taaaaTCCGGCATCCGTCCCCAAT‑CCTGGTTGGGCTTCGCC‑ATAAATAAGCCAGTTTTT‑ATCCCT‑TTT‑ATCCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGan                                                                <  4:1/148‑2 (MQ=255)
                                                                                         aaaTACGGCATCCGTTCTCAAT‑CCTGGTTTTGCTTCGCC‑ATTAATAAACAAGGGTTT‑ATCACC‑TTT‑ATTCACAACCGCCGCACCATCCGTTCCGGACCAGGGCGTTTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGacaac                                                             >  4:673960/1‑151 (MQ=255)
                                                                                           atccGGCATCCGTTCGCAAT‑CCTGGTTTGGCTTCGCC‑ATTAATAACCCAGGTTTT‑ATCCCT‑TTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAg                                                           <  1:165163/148‑1 (MQ=255)
                                                                                            agcGGCATCCGTTCACAA‑‑CCAGGGTTGGTTTGGCCAATTAATACGCCAGTTTTC‑AACCCC‑TTT‑TTCCAAACCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGa                                                          <  4:716607/149‑1 (MQ=255)
                                                                                             ccGGCTTCCTTTCGCAAT‑CCTGTTTTGGCTTCCCC‑ATTAATAACCCAGTTTTT‑ATCCCC‑TTT‑ATCCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGaa                                                         <  4:912931/150‑1 (MQ=255)
                                                                                              cGGCATCCGTTCGCAAT‑CCTGGTTTGGCTTCGCC‑ATTAATAAGCCAGGTTTT‑ATCACC‑TTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGaa                                                         >  2:687693/1‑150 (MQ=255)
                                                                                                  accggTTCGCAAT‑CCTGGTTTGGCTTCCCC‑ATTAATAAGCCGGGTTTA‑ATCCCC‑TTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTa                                                    <  2:108465/147‑1 (MQ=255)
                                                                                                  aTCCGTTCGCAAT‑CCTGGTTTGGCTTCGCC‑ATTAATAAGCCAGGTTTT‑ATCACC‑TTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACtt                                                     >  2:467838/1‑150 (MQ=255)
                                                                                                      gTTCGCAAT‑CCTGGTTTGGCTTCGCC‑ATTAATAAGCCAGGTTTT‑ATCACC‑TTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTga                                                >  3:13624/1‑151 (MQ=255)
                                                                                                      gTTCGCAAT‑CCTGGTTTGGCTTCGCC‑ATTAATAAGCCAGGTTTT‑ATCACC‑TTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTg                                                 >  2:358968/1‑150 (MQ=255)
                                                                                                        ccGCAAC‑CCTGTTTTGGTTCCGCC‑TTTAATAAGCCAGTTTTT‑ACCCCT‑TTT‑ATCCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTgaag                                              <  2:517023/150‑1 (MQ=255)
                                                                                                         cccaaacccTGTTTGGGCTTCGCA‑TAAAAAAAGCCAGTTTTT‑ATCCCC‑TTA‑ATCCCCAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTgaag                                              <  2:189337/144‑1 (MQ=255)
                                                                                                           gcatcccggttttGGCTGCGCC‑ATTAATAAGCCAGTTTTT‑ATCCCC‑TTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTgaagaa                                            <  2:132267/141‑1 (MQ=255)
                                                                                                           cAAT‑CCTGGTTTGGCTTCGCC‑ATTAATAAGCCAGGTTTT‑ATCACC‑TTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGaaa                                           >  1:338536/1‑151 (MQ=255)
                                                                                                            aaT‑CCTGGTTTGGCTTCGCC‑ATTAATAAGCCAGGTTTT‑ATCCCC‑TTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAaat                                          <  2:56049/151‑1 (MQ=255)
                                                                                                            aaT‑CCTGGTTTGGCTTCGCC‑ATTAATAAGCCAGGTTTT‑ATCACC‑TTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAaat                                          >  4:135138/1‑151 (MQ=255)
                                                                                                             aT‑CCTGGTTTGGCTTCGCC‑ATTAATAAGCCAGGTTTT‑ATCACC‑TTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAaata                                         >  1:301685/1‑151 (MQ=255)
                                                                                                              acccTGTTTTGGCTTCCCC‑ATAAATAAGCCAGTTTTT‑ATCCCC‑TTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAaata                                         <  2:919392/149‑1 (MQ=255)
                                                                                                                  tGGTTTGGCTTCGCC‑ATTAATAAGCCAGGTTTT‑ATCACC‑TTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAAtct                                     >  2:1303128/1‑151 (MQ=255)
                                                                                                                   ggTTTGGCTTCGCC‑ATTAATAAGCCAGGTTTT‑ATCACC‑TTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAAtctt                                    >  2:1330494/1‑151 (MQ=255)
                                                                                                                     tttGGTTTCGCC‑ATTAATAAGCCAGTTTTT‑ATCCCC‑TTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAAtcttc                                   <  1:329973/150‑1 (MQ=255)
                                                                                                                      ttGGCTTCGCC‑ATTAATAAGCCAGGTTTT‑ATCACC‑TTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTg                                 >  4:603261/1‑151 (MQ=255)
                                                                                                                        ggCTTCGCC‑ATTAAAAAGCCAGGTTTT‑ATCCCT‑TTT‑ATTCACACCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGc                                <  2:747694/150‑1 (MQ=255)
                                                                                                                          cTTCGCC‑ATTAATAAGCCAGGTTTT‑ATCACC‑TTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCttt                             >  2:1297536/1‑151 (MQ=255)
                                                                                                                            tCGCC‑ATTAATAAGCCAGGTTTT‑ATCACC‑TTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCttttt                           >  4:1197805/1‑151 (MQ=255)
                                                                                                                             cGCC‑ATAAATAAGCCAGGTTTT‑ATCCCC‑TTT‑ATCCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCttttt                           <  2:1468515/150‑1 (MQ=255)
                                                                                                                              gCC‑ATTAATAAGCCAGGTTTT‑ATCACC‑TTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTg                         >  4:250548/1‑151 (MQ=255)
                                                                                                                               cc‑ATTAATAAGCCAGGTTTT‑ATCACC‑TTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGt                        >  2:1228685/1‑151 (MQ=255)
                                                                                                                                c‑ATTAATAAGCCAGGTTTT‑ATCACC‑TTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTc                       >  2:1347296/1‑151 (MQ=255)
                                                                                                                                c‑ATTAAAAAGCGAGTTTTT‑ATCCCC‑TTT‑ATCCACACCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTc                       <  2:338457/151‑1 (MQ=255)
                                                                                                                                  aTTAACAAGCAAGTTTTT‑GTCCCT‑TTT‑TTTCAAAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTc                       <  4:351705/150‑1 (MQ=255)
                                                                                                                                   ttAATAAGCCAGGTTTT‑ATCACC‑TTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCa                      >  4:532919/1‑150 (MQ=255)
                                                                                                                                   ttAATAAGCCAGGTTTT‑ATCACC‑TTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCa                      >  2:1286549/1‑150 (MQ=255)
                                                                                                                                    taataaGCCGGTTTTT‑ATCCCC‑TTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCaa                     <  2:705472/150‑1 (MQ=255)
                                                                                                                                     aataaGCCAGGTTTT‑ATCACC‑TTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCaaa                    >  2:1197016/1‑150 (MQ=255)
                                                                                                                                     aataaGCCAGGTTTT‑ATCACC‑TTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCaaa                    >  4:1016938/1‑150 (MQ=255)
                                                                                                                                           ccAGTTTTT‑ATCCCC‑TTT‑ATTCAAAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGa             <  2:911469/151‑1 (MQ=255)
                                                                                                                                            cAGGTTTT‑ATCACC‑TTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTg              >  4:1209571/1‑149 (MQ=255)
                                                                                                                                               gTTTT‑ATCACC‑TTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCAt         >  2:1335803/1‑151 (MQ=255)
                                                                                                                                                 ttt‑ATCACC‑TTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTg       >  3:317028/1‑151 (MQ=255)
                                                                                                                                                     aTCCCC‑TTT‑ATTCAAAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGAtt    <  1:25755/151‑1 (MQ=255)
                                                                                                                                                        aCC‑TTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGAtttt  >  2:35671/1‑150 (MQ=255)
                                                                                                                                                        |                                                                                                                                                       
TCCCCGCAAAACCGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAA‑TTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAAT‑CCTGGTTTGGCTTCGCC‑ATTAATAAGCCAGGTTTT‑ATCACC‑TTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTT  >  NC_000913/4537961‑4538258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑