Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913357,8900CA43.8% 125.9 / 56.7 105L267F (TTG→TTT) cynRtranscriptional activator of cyn operon; autorepressor
Reads supporting (aligned to +/- strand):  ref base C (42/17);  new base A (0/46);  total (42/63)
Fisher's exact test for biased strand distribution p-value = 1.03e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

Only 100 of 109 total aligned reads displayed.

GATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCC‑AA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGC‑TGGTAACAATGT‑GGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCAATGA  >  NC_000913/357742‑358038
                                                                                                                                                      |                                                                                                                                                        
gATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCC‑AA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGcaaca                                                                                                                                                         >  2:723870/1‑150 (MQ=255)
 aTAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCC‑AA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGcaacaa                                                                                                                                                        >  4:904540/1‑150 (MQ=255)
 aTAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCC‑AA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACaaa                                                                                                                                                       <  2:368107/151‑1 (MQ=255)
 aTAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCC‑AA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACaaa                                                                                                                                                       <  2:1324119/151‑1 (MQ=255)
   aaGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCC‑AA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACaaaa                                                                                                                                                      <  2:707722/150‑1 (MQ=255)
     gCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCC‑AA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAAccg                                                                                                                                                   >  4:761616/1‑151 (MQ=255)
     gCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCC‑AA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAAccg                                                                                                                                                   >  2:991939/1‑151 (MQ=255)
     gCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCC‑AA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAAccg                                                                                                                                                   >  4:262137/1‑151 (MQ=255)
      cGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCC‑AA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAAccgc                                                                                                                                                  >  2:360030/1‑151 (MQ=255)
         aGCGCATCAGGCAATTCCAGCCGCAGCCTTGTTTCAGCGGCACCAGTGATTCATTCCCGCC‑AA‑CACCCGCGCATTAATCCAACTCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAAccgccg                                                                                                                                                <  4:217965/150‑1 (MQ=18)
         aGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCC‑AA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGt                                                                                                                                               >  2:1447584/1‑151 (MQ=255)
           cgcATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCC‑AA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTtc                                                                                                                                             >  2:1422943/1‑151 (MQ=255)
              aTCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCC‑AA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTtctct                                                                                                                                          >  2:449078/1‑151 (MQ=255)
                 aGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCC‑AA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCa                                                                                                                                       >  1:457302/1‑151 (MQ=255)
                     atttccccccGCAGCCGTGTGTCGGCGCCTCCCGTGTTTCTTTCCCCCC‑AC‑CACCCGCGCTTTTACCCAACCCCTTGTGCAAAAATCCCTCCGCGGCGGCTGTCTGCCGGCTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCagtag                                                                                                                                   <  4:1367532/143‑1 (MQ=255)
                     atttCCAGCCGAAGACCTGTGTCAGCGGCTCCCGTGATTCATTCCCGCC‑AA‑CACCCGCGCTTTTATCCACCGCCATGTGCAAAAATGCTTTCGGGGCGGCTGTCTGCCACCTTTTTTCCCGCCGCAACAAAACCGCCGTTCTCTCCagtag                                                                                                                                   <  1:525304/149‑1 (MQ=18)
                     aaTTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCC‑AA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCagt                                                                                                                                     >  4:730292/1‑149 (MQ=255)
                         ccAGCCTCAGCCCTGTGTCAGGGGCTCCCGTGATTAATTCCCGCC‑AA‑CACCCGCGCATTTACCCAACCCCTTGTGAAAAAATCCCTCCGGGGCGGCTGTCTGCCAGTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGc                                                                                                                               <  4:29075/151‑1 (MQ=255)
                         ccAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCC‑AA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGc                                                                                                                               >  4:972568/1‑151 (MQ=255)
                          cAGCCGCAGCCCTGTGCCAGCGGCTACCGTTATTCTTTCCCGCA‑AA‑CACCCGCGCATTTACCCAACCCCATGTGAAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTTTTTTTCCCCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCg                                                                                                                              <  2:789862/151‑1 (MQ=255)
                          cAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCC‑AA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCg                                                                                                                              >  2:1423815/1‑151 (MQ=255)
                           aGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCC‑AA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCgg                                                                                                                             >  4:848652/1‑151 (MQ=255)
                               gCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCC‑AA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCgggggg                                                                                                                         >  4:1106068/1‑149 (MQ=255)
                               gCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCC‑AA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCgggggg                                                                                                                         >  2:212207/1‑149 (MQ=255)
                               ccggACGTGTGAGAGGGGCGACTGTGATCCTTTCCCGCC‑AA‑CAACCGCGCATTAATCCAGCGCGATGTAAAAAAATGCCTTCCGGGCGGCTGTCTACCGGGTATTTGTCCGCCGAAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCa                                                                                                                         <  2:1393002/148‑1 (MQ=16)
                               ccagccttgtgTCGGCGGCTACCGTGTTTCTTTCCCCCC‑AA‑CACCCGCGCTTTTACCCACCGCCATGTGAAAAAATGCCTCCGCGGCGGCTGCCGGCCAGTTATTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCa                                                                                                                         <  4:1233297/144‑1 (MQ=255)
                                 agcccTGTGTCAGCGGCTCCCGTGTTTCATTCCCGCC‑AA‑CACCCGCGCATTTATCCAACGCCATGTGAAAAAATGCCTTCGCGGCGGCTGTCTGCCAGTTATTTTTCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAag                                                                                                                       <  2:1421307/148‑1 (MQ=255)
                                  gACTTGTGTCAGCGGCTCCCGT‑ATTCCATTCCCCC‑AACCACCCGCGCTTTTACCCACCCCCATGTGAAAAAATCCCTCCGCGGCGCCTGTCTGCCAGCTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAaga                                                                                                                      <  4:1040781/151‑1 (MQ=255)
                                  gACCTGTGTCAGGGGCTACCGTGTTTCATTTCCCCC‑AA‑CAACCGCGCATTTACCCAACGCCATGTGAAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAaga                                                                                                                      <  2:883025/151‑1 (MQ=255)
                                   actggtgtCACCGGCTCCCGTGATTCATTCCCCCA‑AA‑CACCCCCGCTTTTATCCACCGCCATGTGCAAAAATGCCTCCGCGGCGGCTGTCGGCAAGCTTTTTTCCCGCGACAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAaga‑g                                                                                                                    <  3:20288/147‑1 (MQ=18)
                                    ccTGTGTCGGCGGCTCCCGTGTTTCATTTCCGCC‑AA‑CAACCGCGCATTTACCCACCGCCATGGGAAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTTTTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAga‑g‑a                                                                                                                  <  2:1423092/151‑1 (MQ=255)
                                     cTGTGTCAGCGGCTACCGTGATTCATTTCCGCC‑AA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCggggggcgaga                                                                                                                    >  4:122787/1‑143 (MQ=255)
                                      tgtgTCAGCGGCTACCGTGATTCATTTCCGCC‑AA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCggggggcggg                                                                                                                     >  4:1051733/1‑142 (MQ=255)
                                       gtgtCAGCGGCTACCGTGATTCATTTCCGCC‑AA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCggggggcgaggaa                                                                                                                  >  4:1216314/1‑141 (MQ=255)
                                       gtgtCAGCGGCTACCGTGATTCATTTCCGCC‑AA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGTGccaaaaaaa                                                                                                                  >  2:1080993/1‑142 (MQ=255)
                                       gtgtCAGCGGCTACCGTGATTCATTTCCGCC‑AA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGTGGACga‑g‑ataa                                                                                                               >  3:474650/1‑148 (MQ=255)
                                         gtCAGCGGCTCCCGTGATTCATTCCCGCC‑AA‑CAACCGCGCATTTACCCAACGCCATGTGAAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTCCCGCCGAAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAg                                                                                                             <  2:982231/151‑1 (MQ=25)
                                         gccggcgcCTCCCGTGTTTCTTTCCCGCC‑AA‑CACCCGCGCTTTTACCCACCCCCATGTGAAAAAATGCTTCCGCGGCGGCTGTCTGCCAGTTATTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAg                                                                                                             <  4:331378/147‑1 (MQ=255)
                                          tCAGCGGCTACCGTGATTCATTTCCGCC‑AA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGAGA‑G‑gaaaaa                                                                                                             >  4:801666/1‑147 (MQ=255)
                                              cGGCTACCGTGATTCATTTCCCCC‑AA‑CACCCGCGCATTTACCCAACGCCATGTGAAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTa                                                                                                        <  2:1233179/151‑1 (MQ=255)
                                               ggCTACCGTGATTCTTTCCCGCC‑AA‑CACCCGCGCTTTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTaa                                                                                                       <  2:1185234/151‑1 (MQ=25)
                                                 cTACCGTGATTCATTCCCGCC‑AA‑CACCCGCGCATTTACCCAACGCCATGTGCAAAAATCCTTTCGCGGCGGCTGTCTGCCACTTATTTTCCCGCCCCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGc                                                                                                     <  1:190382/151‑1 (MQ=25)
                                                  tACCGTGATTCATTTCCGCC‑AA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCGAGA‑G‑AAAAAACttttaacc                                                                                                    >  2:1284696/1‑146 (MQ=255)
                                                   cccgtttttCTTTCCCCCC‑AC‑CACCCGCGCATTTCCCCACCGCCTTGTAAAAAAATGCTTCCGCGGCGGCTGTCTGCCAGTTATTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGc                                                                                                     <  4:1422481/142‑1 (MQ=255)
                                                   cccGTGTTTCATTTCCGCC‑AA‑CAACCGCGCTTTTACCAAACCCCATGTGCAAAAAGGCTTCCGCGGCGGCTGTCTCCCAGCTATTTTCCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGc                                                                                                     <  2:1104562/148‑1 (MQ=255)
                                                   aCCGTGATTCATTTCCGCC‑AA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGAAGA‑G‑aaaaaaacttaaacc                                                                                                    >  1:414803/1‑138 (MQ=255)
                                                    ccgtatttCATTCCCGCC‑AA‑CACCCGCGCTTTTACCCACCGCCATGTGAAAAAATGCTTCCGGGGCGGCTGTCTGCCAGCTATTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCg                                                                                                  <  4:1364152/145‑1 (MQ=255)
                                                      gTGATTCATTTCCGCC‑AA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGAAGA‑G‑AAAAAACttttaaaccccc                                                                                                >  2:628715/1‑142 (MQ=25)
                                                        gATTCATTTCCGCC‑AA‑CACCCGCGCATTTATCCAACGCCATGTGCAAAAATGCTTTCGCGGCGGCTGTCTCCCAGCTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCAt                                                                                              <  2:912299/151‑1 (MQ=37)
                                                          ttCATTCCCGCC‑AC‑CACCCGCGCTTTTACCCAACCCCATGTGAAAAAATGCTTTCGCGGCGGCTGTCTGCCAGTTATTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCAtg                                                                                             <  4:439113/150‑1 (MQ=255)
                                                            cATTTCCGCC‑AA‑CACCCGCCCATTTACCCAACGCCATGTGCAAAAATGCCTCCGCGGCGGCTTTCTGCCAGTTATTTTCCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCAtgtt                                                                                           <  2:1215652/150‑1 (MQ=38)
                                                            cATTCCCCCC‑AA‑CACCCGCGCATTTACCCACCGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCAtgttg                                                                                          <  1:358644/151‑1 (MQ=37)
                                                             ctttCCCCCAAA‑CACCCGCTCATTTACCCACCGCCATGGCAAAAAATGCCTCCGCGGCGGTGGCCTGCAGGCTTTTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCAtgttg                                                                                          <  3:69406/150‑1 (MQ=255)
                                                             aTTTCCGCC‑AA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGAGAGG‑AAAAAGATTTAAccccccctgtttt                                                                                          >  4:722925/1‑142 (MQ=25)
                                                               ttCCCCC‑AA‑CACCCGCGCTTTTACCCAACGCCATGTGAAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTtgtg                                                                                        <  2:498897/150‑1 (MQ=255)
                                                                cccccc‑AA‑CACCCCCGCTTTTTCCCACCCCCATGTGAAAAATTGCCTTCGCGCCGGCTGCCTCCCGGCTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTtgtgtg                                                                                      <  2:1417728/147‑1 (MQ=255)
                                                                 ccccc‑AA‑CACCCGCGCATTTACCCAACGCAATGTGAAAAAATGCCTCCGCGGCGGCTGTCGGCCAGTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGTGTgg                                                                                     <  1:551792/148‑1 (MQ=255)
                                                                 ccGCC‑AA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGAGA‑G‑AAAAAAATTTTAAACCGCCATGTTTTTTgg                                                                                     >  2:1289536/1‑151 (MQ=25)
                                                                    ccaaa‑caccCGCGCATTTACCCAACCCCATGTGAAAAAAGCCCTTCGCGGCGGCTGTCTGCCGGCTTTTTTTCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCa                                                                                   <  2:896112/148‑1 (MQ=255)
                                                                      caaaaacccGCGAATTTACCCACCGCCATGTGAAAAAAGGCCTTCGGGGCGGCTGTCGGCCAGCTATTTTTCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCaa                                                                                  <  1:224229/144‑1 (MQ=255)
                                                                       aa‑caccCGCGCTTTTACCCACCGCCATGTGAAAAAATGCCTTCGCGGCGGCTGTCTGCCAGTTATTTTTCCGCCGCAAAAAAACCGCCGTTCTCTCCagtagt                                                                                                                                  <  2:669551/103‑1 (MQ=255)
                                                                       aa‑caacCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCagtagt                                                                                                                                  >  4:669548/1‑103 (MQ=255)
                                                                          cacccGCGCTTTTACCCACCGCCATGTCCAAAAAGCCCTCCGGGGCGGCTGCCTGCCAGTTTTTTTCCCCCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCgc                                                                              <  4:1336974/148‑1 (MQ=255)
                                                                               gctcATTTCCCCACCGCCTTGTAAAAAAATGCCTCCGGGGCGGTTCGCCCCCCCTTTTTTTCCCCCCGAAAAAAACCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGAAATAGctgg‑tg                                                                        <  4:830259/148‑1 (MQ=255)
                                                                                 gcttttaccaaccGCCATGTAAAAAAATGCTTTCGCGGCGGCTGTCTGCAGGTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGC‑TGGt                                                                      <  2:1207005/139‑1 (MQ=255)
                                                                                  tatttccccAGCGCTATGTGCAAAAATGCCTTCGGGGCGGTTGTTTACAAGATATTTTTCCGCCCCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGC‑TGGt                                                                      <  2:1427528/143‑1 (MQ=255)
                                                                                  cATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAAAAAAATTTATCCCGTCATATTTTGTGGGaaaggcttccggg                                                                       >  2:77141/1‑139 (MQ=25)
                                                                                   aTTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGCAGG‑G‑AAAAAAGTTTTAACCCCCCCTGTTGTGTgggggtcgcggccgggg                                                                      >  4:1460577/1‑135 (MQ=25)
                                                                                    tttaccaacccccATGTGAAAAAATGCCTGCGCGGGGGCTTTCGGCCAGCTTTTTTCCCGCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGC‑TGGTaa                                                                    <  4:546657/139‑1 (MQ=255)
                                                                                    tttACCCACCCCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTCCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGC‑TGGTAAc                                                                   <  1:91023/151‑1 (MQ=255)
                                                                                      tacccacccccATGTGCAAAAAGGCCTCCGCGGCGGTTGTCTGCCAGTTATTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGC‑TGGTAACaa                                                                 <  1:514636/142‑1 (MQ=255)
                                                                                       acccAACGCCATGTAAAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTTTTTTCCCGCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGc                                                                                                     <  3:436155/113‑1 (MQ=255)
                                                                                        ccaaccGCAATGTGAAAAAAGGCTTCCGGGGCGGCTTTCTGCCGGCTATTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGC‑TGGTAACAAtg                                                               <  3:419756/146‑1 (MQ=255)
                                                                                              cccATGTGAAAAAATGCCTTCGCGGCGGCTGTCTGCCAGTTATTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGC‑TGGTAACAATGT‑GGaaa                                                        <  3:87145/150‑1 (MQ=255)
                                                                                               ccATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGAAGA‑A‑AAAAAGATTTTAGCCCGGCACGTTGTGTGGGAAACGCTGCTTGGTACCaaaaggaa                                                           >  2:232322/1‑143 (MQ=37)
                                                                                                    tgCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGAGA‑G‑AAAAAAATTTTAACCCCCCCCGTTGTGTGGGGATCGCTGC‑TGGTGACAATGT‑GGGAAgggg                                                    >  4:1458743/1‑148 (MQ=255)
                                                                                                     gCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGCAGG‑G‑AAAAAAATTTTAAACCCCCCTTTTGTGTGGGGATCCCTGC‑CGGGCACAAAAT‑GGGAAAGGAAgg                                                 >  4:1066061/1‑150 (MQ=255)
                                                                                                      cAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAAA‑G‑AAAAAAATTTTAAACCCCCATGTTGTGTGGGAATCGCTGC‑TGGGAACAATGT‑Gggaagggaag                                                  >  2:412348/1‑149 (MQ=255)
                                                                                                        aaaaTGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGCGG‑G‑AAAAAGCATTAAGCCCGCCCTGTTTTTTGGCGATCGCCGC‑GGGTGACAATGT‑GGGAAGGGagggggg                                              >  4:65584/1‑145 (MQ=255)
                                                                                                        aaaaTGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGAAGAGG‑AAAAAACTTTAA‑CCCCCCATGGTTTGTGGGGAACGCCGC‑TTGGAAACATTT‑GGGAAGGGAAGGgcg                                              >  4:1139846/1‑151 (MQ=255)
                                                                                                             gctttCGGGGCGGCTGTCTCCCAGCTTTTTTCCCCCCACAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGC‑TGGTAACAATGT‑GGAAAGGGAAGTGCGGCGa                                          <  2:1226729/147‑1 (MQ=255)
                                                                                                              ccTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGGAA‑GAAAAAAACTTTAA‑AACGCCATGTTGTGTGGGGATAGCCGC‑TGGGAAAAAAGT‑GGGAAGGGGAggggggggga                                         >  2:132701/1‑141 (MQ=255)
                                                                                                                 ccgcgGCGGTTGTCTGCAGGTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGC‑TGGTAACAATGT‑GGAAAGGGAAGTGCGGCGAATCAg                                     <  4:1423818/150‑1 (MQ=255)
                                                                                                                    ggggggcTGTCTGCCAGCTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGC‑TGGTAACAATGT‑GGAAAGGGAAGTGCGGCGAATCAGCTc                                  <  4:1271465/147‑1 (MQ=255)
                                                                                                                      ggggcTTTCTGCCGGTTTTTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGC‑TGGTAACAATGT‑GGAAAGGGAAGTGCGGCGAATCAGCTCCa                                <  4:1243140/149‑1 (MQ=255)
                                                                                                                          cTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGCAGA‑G‑AAAAAAATTTTAACCCCCCCTTTTTTGTGGGAATAGCTTC‑TGGTGACAATGTGGGAAAGGGAGGGGGGGGGAA‑CAGCTCCaaaa                             >  2:780670/1‑150 (MQ=255)
                                                                                                                            gccTGCCAGCTTTTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGC‑TGGTAACAATGT‑GGAAAGGGAAGTGCGGCGAATCAGCTCCAGAAc                            <  2:1297782/147‑1 (MQ=255)
                                                                                                                            gTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCCAGA‑G‑AAAAAACTTTAAACCCCGCAAGTTGTGTGGGGATTGCCGC‑TGGTGAAAAAGT‑GGGAAGGGGAGGGCGGCGGAACAGCTCCAGAAccc                          >  2:574983/1‑150 (MQ=255)
                                                                                                                                gCCAGCTATTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGC‑TGGTAACAATGT‑GGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACcgcg                        <  4:183887/149‑1 (MQ=255)
                                                                                                                                   agtttttttcCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGC‑TGGTAACAATGT‑GGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAAt                   <  2:801669/146‑1 (MQ=255)
                                                                                                                                   aGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAAA‑G‑GAAAAACTTTTAAACCCCCATGTTGTGGGGGGAATGCCGG‑TGGTAACAATGT‑GGGAAGGGAAGGGCGGGGGAACAACTCCCGAAACgcgcccat                   >  2:467379/1‑147 (MQ=255)
                                                                                                                                      ttttttcCCGCCGAAAAAAATCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGC‑TGGTAACAATGT‑GGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTg                 <  4:1248303/148‑1 (MQ=255)
                                                                                                                                       atttcgccgccgCAACAAAGCCGCCGTTCTCTCCAGTAGTTGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGC‑TGGTAACAATGT‑GGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAg               <  4:1313127/145‑1 (MQ=255)
                                                                                                                                       aTTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGTGGCAGA‑G‑AAAAAAACTTAAAACCCTCCCGTTGTGTGGGGGTCCCTGC‑TGGGGACAAAAT‑GGGAAGGGGAAGGGGGCGGAGCACCTCCCGAACCCCGCCaaatta                >  4:171259/1‑146 (MQ=255)
                                                                                                                                         ttttCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGAAGA‑G‑AAAAAAAATTAAACCCCTCCTGTTTTGTGGGAATTGCTGG‑TGGTGAAAAAGT‑GGGAAGGGGAGGGGGGGGGAACAACTCCCCAACCGCGCCAAATGAggt             >  2:1463101/1‑149 (MQ=255)
                                                                                                                                         tttcCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGC‑TGGTAACAATGT‑GGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGtt             <  1:178900/151‑1 (MQ=255)
                                                                                                                                           tcccgccgCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGTGTAGCAATCGCTGC‑TGGTAACAATGT‑GGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTc            <  4:306493/148‑1 (MQ=255)
                                                                                                                                            cccgccgCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGC‑TGGTAACAATGT‑GGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCg           <  2:1290294/149‑1 (MQ=255)
                                                                                                                                             ccgccgCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGAAGA‑G‑GAAAAAAATTAAACCCCCCCTGTTGTGTGGGGATAGCTGC‑TGGTGACAATGT‑GGAAAGGGGAGGGGGGGGGAGCAGCCCCAGAACCGCGCCAATTTAGTTTGcc         >  4:1463095/1‑151 (MQ=255)
                                                                                                                                                    aacaaAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGC‑TGGTAACAATGT‑GGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCAATGa  <  2:848661/151‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                        
GATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCC‑AA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGC‑TGGTAACAATGT‑GGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCAATGA  >  NC_000913/357742‑358038

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑