Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913501,5750AC48.5% 225.9 / 23.7 132V555G (GTG→GGG) ybaLinner membrane putative NAD(P)‑binding transporter
Reads supporting (aligned to +/- strand):  ref base A (62/5);  new base C (0/63);  total (62/70)
Fisher's exact test for biased strand distribution p-value = 1.18e-31
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 140 total aligned reads displayed.

TTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAATCTCAATATCCGGATTTT  >  NC_000913/501426‑501725
                                                                                                                                                     |                                                                                                                                                      
ttAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTcaccac                                                                                                                                                       >  4:70124/1‑151 (MQ=255)
ttAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTcaccac                                                                                                                                                       <  3:281800/151‑1 (MQ=255)
 tAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTcaccac                                                                                                                                                       <  2:1042844/150‑1 (MQ=255)
 tAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTcaccac                                                                                                                                                       <  2:17409/150‑1 (MQ=255)
    gTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCATCGTTTACCCCGTCACCACCTCa                                                                                                                                                   >  3:547131/1‑151 (MQ=255)
    gTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTc                                                                                                                                                    >  2:270999/1‑150 (MQ=255)
         cgtcgGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACccgc                                                                                                                                              >  2:1051749/1‑151 (MQ=255)
           tcgGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACccgccg                                                                                                                                            >  1:400240/1‑151 (MQ=255)
              gTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTg                                                                                                                                         >  2:637056/1‑151 (MQ=255)
                tCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTgg                                                                                                                                        >  1:330283/1‑150 (MQ=255)
                tCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGc                                                                                                                                       >  4:1299877/1‑151 (MQ=255)
                 cGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCg                                                                                                                                      >  4:205431/1‑151 (MQ=255)
                  gTAGCCCAGATAAGGCTTTCCCGCCGCATCTGGCTTTTGGCTCTCGAGGCCTGATGCGCCGCTGGCGCGTCTTATCATGCCTACATTTTTTCTATATTTTCCATCGGGCACCCCCCGTTTCCCCCGTCACCCCCTCACCCGCCGGTGGCGt                                                                                                                                     <  4:440358/151‑1 (MQ=25)
                     ggCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGttt                                                                                                                                   >  3:459688/1‑150 (MQ=255)
                     ggCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGttt                                                                                                                                   >  1:469834/1‑150 (MQ=255)
                     ggCCAAAAAGGGCTTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCGGGCGCTTCTTATCATCCCTACATTTTTTTCATATTTTACATCCGGCAACCACCGTTTCCCCCGTCCCCCCCTCCCCCGCCGGTGGCGTTTc                                                                                                                                  <  4:1029850/151‑1 (MQ=255)
                       ccAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCca                                                                                                                                >  1:86795/1‑151 (MQ=255)
                        cAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCcag                                                                                                                               >  3:390435/1‑151 (MQ=255)
                           aTAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCcagc                                                                                                                              >  4:467553/1‑149 (MQ=255)
                               ggCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTc                                                                                                                         >  1:520601/1‑150 (MQ=255)
                               ggCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTCACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTc                                                                                                                         >  3:166632/1‑150 (MQ=255)
                                    tCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCAt                                                                                                                   >  2:1378952/1‑151 (MQ=255)
                                      aCGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATgg                                                                                                                 >  4:46067/1‑151 (MQ=255)
                                                 ggCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGAtt                                                                                                      >  2:417037/1‑151 (MQ=255)
                                                     tttGTCTCTCGTTGCCTGATGCGACGCTGGCGCGTTTTATCATGCCTACATATTTTTCATATTTTACATCCGGCACCCACCGTTTCCCCCCTCACCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGAttt                                                                                                     <  4:1327665/148‑1 (MQ=38)
                                                       tGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACACCCGGAACCCCCCGTTTCCCCCGTCACCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGc                                                                                                <  1:376733/151‑1 (MQ=38)
                                                        ggCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCCCCTTTTCCCCCGTCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGc                                                                                                <  2:900627/150‑1 (MQ=39)
                                                            ctcGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGcc                                                                                           >  2:894831/1‑151 (MQ=255)
                                                            ctcGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGc                                                                                            >  3:350204/1‑150 (MQ=255)
                                                            ctcGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGc                                                                                            >  3:276610/1‑150 (MQ=255)
                                                               gATGCCTGATGCGACGCTGGTGCGTCTTATCATCCTTACATATTTTTCATATTTTACCTCCGGCACCCCCCGTTTCCCCCGTCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCAt                                                                                        <  4:1454781/151‑1 (MQ=255)
                                                               gATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCAt                                                                                        >  2:1018353/1‑151 (MQ=255)
                                                                aTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATTTTTTTCATATTTTACACCCGGCACCCCCCGTTTCCCCCGTCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATc                                                                                       <  4:484076/151‑1 (MQ=38)
                                                                   ccTGATGCGACGCGGGCGCGTTTTATCATGCCTACATATTTTTCATATTTTACACCCGGCAACCACCGTTTCCCCCGTCCCCCCCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACt                                                                                    <  2:263585/151‑1 (MQ=255)
                                                                     tGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTAc                                                                                  >  4:1084029/1‑151 (MQ=255)
                                                                       aTGCGACGCTGGCGCTTTTTATCATGCCTACATATTTTTCATATTTTCCCTCCGGCAACCACCGTTTCCCCCGCCCCCACCCCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCt                                                                                <  4:848844/151‑1 (MQ=255)
                                                                       aTGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCCCCTTTTCCCCCGTCCCCCCCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTAcc                                                                                 <  1:541512/150‑1 (MQ=255)
                                                                        tGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTg                                                                               >  4:1277427/1‑151 (MQ=255)
                                                                         gCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTg                                                                               >  4:1395498/1‑150 (MQ=255)
                                                                           gACGCTGGCGCGTCTTATCATGCCTACATTTTTTTCATATTTTACACCCGGCACCCACCTTTTCCCCCGTCCCCCCCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGAtt                                                                            <  2:802808/151‑1 (MQ=255)
                                                                            ccGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGCCACCCACCTTTTCCCCCGTCCCCCCCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTc                                                                           <  2:1058383/150‑1 (MQ=255)
                                                                                 ggCGCTTCTTATCATCCCTACATTTTTTTCATTTTTTACACCCGGCACCCCCCTTTTCCCCCGTCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGcacc                                                                      <  2:146643/151‑1 (MQ=255)
                                                                                   cgctttttctCATGCCTACATTTTTTTCATTTTTTACTTCCGGCACCCACCTTTTCCCCCGTCCCCACCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCACCACTACCTGATTCGcaccac                                                                    <  4:240832/142‑1 (MQ=255)
                                                                                      gTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGtt                                                                 >  3:135306/1‑151 (MQ=255)
                                                                                       tCTTATCATGCCTACATCTTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTc                                                                >  2:187653/1‑151 (MQ=255)
                                                                                       tCTTATCATCCCTACATTTTTTTCATTTTTTCCACCCGGCACCCCCCGTTTCCCCCGTCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGtt                                                                 <  4:243710/150‑1 (MQ=255)
                                                                                        cTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCg                                                               >  4:402612/1‑151 (MQ=255)
                                                                                         ntATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCgg                                                              >  4:159043/2‑151 (MQ=255)
                                                                                          ggcattccttccATTTTTTGCATATTTAGCACCCGGCACCCACCTTTCGCCCTGTCGCCGCCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTGCCTGATTCGCACCACGTTCGGt                                                             <  2:522519/140‑1 (MQ=255)
                                                                                           aTCATGCCTACATATTTTTCATATTTTACATCCGGCACCCACCGTTTCCCCCGTCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTg                                                            <  3:154323/151‑1 (MQ=255)
                                                                                           aTCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTg                                                            >  4:312689/1‑151 (MQ=255)
                                                                                           aTCATGCCTACATATTTTTCATATTTTACACCCGGCAACCCCCGTTTCCCCCGTCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTg                                                            <  2:27741/151‑1 (MQ=255)
                                                                                            tCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTg                                                            >  4:722375/1‑150 (MQ=255)
                                                                                             cATGCCTACATTTTTTTCATATTTTACATCCGGCACCCCCCGTTTCCCCCGTCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGa                                                           <  4:1402305/150‑1 (MQ=255)
                                                                                             cATGCCTACATTTTTTGCATATTTTACACCCGGCAACCACCGTTTCCCCGGTCCCCACCGCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGat                                                          <  2:1350319/151‑1 (MQ=255)
                                                                                             cATCCCTACTTATTTTTTATTTTTTACACCCGGCACCCCCCGTTTCCCCCTTCCCCCCCTCGCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGat                                                          <  2:1225988/151‑1 (MQ=255)
                                                                                              aTGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGat                                                          >  2:731105/1‑150 (MQ=255)
                                                                                                gCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGAtata                                                       >  3:22456/1‑151 (MQ=255)
                                                                                                 ccTCCATATTTTTCATATTTTCCACCCGGAACCCACCGTTTCCCCCGTCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTGCCTGATTCGCACCACGTTCGGTGATATAc                                                      <  3:187370/151‑1 (MQ=255)
                                                                                                 ccTACATTTTTTTCATATTTTACATCCGGCAACCCCCGTTTCCCCCGTCACCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATAc                                                      <  2:383043/151‑1 (MQ=255)
                                                                                                 ccTACATTTTTTTCATATTTTACACCCGGCACCCCCCTTTTCCCCCGTCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATAc                                                      <  3:172414/151‑1 (MQ=255)
                                                                                                   tACATTTTTTTCATATTTTCCACCCGCCACCCCCCGTTTCCCCCGTCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGc                                                    <  3:469497/151‑1 (MQ=255)
                                                                                                    aCATATTTTTCATATTTTACACCCGGCACCCCCCGTTTCCCCCGTCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACg                                                     <  2:247170/149‑1 (MQ=255)
                                                                                                         tttttCATATTTTACACCCGGCACCCACCTTTTCCCCCGTCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTc                                              <  3:411062/151‑1 (MQ=255)
                                                                                                           tttCATATTTTCCACCCGGCAACCCCCGTTTCCCCCGTCCCCCCCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCAt                                            <  1:8119/151‑1 (MQ=255)
                                                                                                            ttCATATTTTACATCCGGCAACCCCCTTTTCCCCCGTCACCACCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATc                                           <  1:462879/151‑1 (MQ=255)
                                                                                                            ttCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATc                                           >  4:1014475/1‑151 (MQ=255)
                                                                                                             tCATATTTTACACCCGGCAACCCCCGTTCCCCCCGTCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCg                                          <  3:435898/151‑1 (MQ=255)
                                                                                                              cATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGt                                         >  2:1283114/1‑151 (MQ=255)
                                                                                                               atatTTTACATCCGGCAACCCCCGTTTCCCCCGTCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTc                                        <  1:470545/151‑1 (MQ=255)
                                                                                                                    ttACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAAt                                   >  4:102069/1‑151 (MQ=255)
                                                                                                                    ttACACCCGGCACCCCCCTTTCCCCCCGTCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAAt                                   <  3:309827/151‑1 (MQ=255)
                                                                                                                     tacccccGGCACCCCCCTTTTCCCCCGTCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATg                                  <  3:171456/146‑1 (MQ=255)
                                                                                                                     tACATCCGGCACCCCCCTTTTCCCCCGTCCCCCCCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAAt                                   <  2:629681/150‑1 (MQ=255)
                                                                                                                     tACACCCGGCAACCCCCGTTTCCCCCGTCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATg                                  <  1:507310/151‑1 (MQ=255)
                                                                                                                     tACACCCGGCAACCCCCGTTTCCCCCGTCACCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAAt                                   <  4:1051745/150‑1 (MQ=255)
                                                                                                                        aTCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGgc                               >  4:1186120/1‑151 (MQ=255)
                                                                                                                         tCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGgcg                              >  4:1156331/1‑151 (MQ=255)
                                                                                                                              cAACCCCCTTTTCCCCCGTCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGc                         <  4:1156858/151‑1 (MQ=255)
                                                                                                                                accaccGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCCCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCa                        >  4:69671/1‑150 (MQ=255)
                                                                                                                                 cccccGTTTCCCCCGTCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCaat                      <  2:512260/148‑1 (MQ=255)
                                                                                                                                 ccaccGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCaat                      >  3:552792/1‑151 (MQ=255)
                                                                                                                                  ccccGTTTCCCCCGTCACCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCaata                     <  2:467549/149‑1 (MQ=255)
                                                                                                                                   cccGTTTACCCCGTCCCCTCCCCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCaataa                    <  2:302226/150‑1 (MQ=255)
                                                                                                                                    ccGTTTCCCCCGTCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCaataat                   <  1:80310/151‑1 (MQ=255)
                                                                                                                                      ttttCCCCCGTCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAAtct                 <  3:432404/150‑1 (MQ=255)
                                                                                                                                      gTTTCCCCCCTCCCCACCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAAtct                 <  4:1027680/151‑1 (MQ=255)
                                                                                                                                       tttACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAAtctc                >  2:822789/1‑151 (MQ=255)
                                                                                                                                        ttcccccctccccccctccCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAAtctc                <  4:858953/147‑1 (MQ=255)
                                                                                                                                        ttACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAAtctc                >  2:1067358/1‑150 (MQ=255)
                                                                                                                                         tcccccGCCCCCCCCCCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAATCTCa               <  4:1074232/148‑1 (MQ=255)
                                                                                                                                          cccccGTCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAATCTCAat             <  3:520596/150‑1 (MQ=255)
                                                                                                                                           ccccGTCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAATCTCAata            <  3:242567/151‑1 (MQ=255)
                                                                                                                                              cGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAATCTCAATATcc         >  2:1098459/1‑151 (MQ=255)
                                                                                                                                               gTCCCCCCCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAAtctc                <  1:2098/143‑1 (MQ=255)
                                                                                                                                               gTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAAtctc                >  3:2098/1‑143 (MQ=255)
                                                                                                                                               gTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAATCTCAATATCCg        >  2:1291415/1‑151 (MQ=255)
                                                                                                                                                tCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAATCTCAATATCCgg       >  4:580271/1‑151 (MQ=255)
                                                                                                                                                tCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGCCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGTAATAATTTCAATATCCgg       >  2:971279/1‑151 (MQ=255)
                                                                                                                                                     accTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAATCTCAATATCCGGAtttt  >  1:322943/1‑151 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
TTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAATCTCAATATCCGGATTTT  >  NC_000913/501426‑501725

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑