Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	850,651	T→G	Y68D (TAC→GAC)	ompX →	outer membrane protein X

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	850,651	0	T	G	100.0%	96.1 / NA	28	Y68D (TAC→GAC)	ompX	outer membrane protein X
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (12/16);  total (12/16)

GCGACTTCTACTGTAACTGGCGGTTACGCACAGAGCGACGCTCAGGGCCAAATGAACAAAATGGGCGGTTTCAACCTGAAATACCGCTATGAAGAAGACAACAGCCCGCTGGGTGTGATCGGTTCTTTCACTTACACCGAGAAAAGCCGTACTGCAAGCTCTGGTGACTACAACAAAAACCAGTACTACGGCATCACTGCTGGTCCGGCTTACCGCATTAACGACTGGGCAAGCATCTACGGTGTAGTGGGTGTGGGTTATGGTAAATTCCAGACC  >  NC_000913/850519‑850794                                                                                                                                     |                                                                                                                                                gCGACTTCTACTGTAACTGGCGGTTACGCACAGAGCGACGCTCAGGGCCAAATGAACAAAATGGGCGGTTTCAACCTGAAATACCGCTATGAAGAAGACAACAGCCCGCTGGGTGTGATCGGTTCTTTCACTGACACCGAGAAAAGCCGTa                                                                                                                               >  3:440846/1‑151 (MQ=255)   gACTTCTACTGTAACTGGCGGTTACGCACAGAGCGACGCTCAGGGCCAAATGAACAAAATGGGCGGTTTCAACCTGAAATACCGCTATGAAGAAGACAACAGCCCGCTGGGTGTGATCGGTTCTTTCACTGACACCGAGAAAAGCCGTa                                                                                                                               >  3:414549/1‑149 (MQ=255)             gTAACTGGCGGTTACGCACAGAGCGACGCTCAGGGCCAAATGAACAAAATGGGCGGTTTCAACCTGAAATACCGCTATGAAGAAGACAACAGCCCGCTGGGTGTGATCGGTTCTTTCACTGACACCGAGAAAAGCCGTACTGCAAGct                                                                                                                      >  1:217069/1‑148 (MQ=255)                    gCGGTTACGCACAGAGCGACGCTCAGGGCCAAATGAACAAAATGGGCGGTTTCAACCTGAAATACCGCTATGAAGAAGACAACAGCCCGCTGGGTGTGATCGGTTCTTTCACTGACACCGAGAAAAGCCGTACTGCAAGCTCTGGTGACt                                                                                                             <  1:526951/150‑1 (MQ=255)                     cGGTTACGCACAGAGCGACGCTCAGGGCCAAATGAACAAAATGGGCGGTTTCAACCTGAAATACCGCTATGAAGAAGACAACAGCCCGCTGGGTGTGATCGGTTCTTTCACTGACACCGAGAAAAGCCGTACTGCAAGCTCTGGTGACTac                                                                                                           <  1:440852/151‑1 (MQ=255)                       gTTACGCACAGAGCGACGCTCAGGGCCAAATGAACAAAATGGGCGGTTTCAACCTGAAATACCGCTATGAAGAAGACAACAGCCCGCTGGGTGTGATCGGTTCTTTCACTGACACCGAGAAAAGCCGTACTGCAAGCTCTGGTGACTacaa                                                                                                         >  1:139092/1‑151 (MQ=255)                                 gagCGACGCTCAGGGCCAAATGAACAAAATGGGCGGTTTCAACCTGAAATACCGCTATGAAGAAGACAACAGCCCGCTGGGTGTGATCGGTTCTTTCACTGACACCGAGAAAAGCCGTACTGCAAGCTCTGGTGACTACAACAAAAACCAg                                                                                               >  1:394382/1‑151 (MQ=255)                                        gCTCAGGGCCAAATGAACAAAATGGGCGGTTTCAACCTGAAATACCGCTATGAAGAAGACAACAGCCCGCTGGGTGTGATCGGTTCTTTCACTGACACCGAGAAAAGCCGTACTGCAAGCTCTGGTGACTACAACAAAAACCAGTACTACg                                                                                        >  3:534421/1‑151 (MQ=255)                                          tCAGGGCCAAATGAACAAAATGGGCGGTTTCAACCTGAAATACCGCTATGAAGAAGACAACAGCCCGCTGGGTGTGATCGGTTCTTTCACTGACACCGAGAAAAGCCGTACTGCAAGCTCTGGTGACTACAACAAAAACCAGTACTACGGc                                                                                      <  1:534426/151‑1 (MQ=255)                                              ggCCAAATGAACAAAATGGGCGGTTTCAACCTGAAATACCGCTATGAAGAAGACAACAGCCCGCTGGGTGTGATCGGTTCTTTCACTGACACCGAGAAAAGCCGTACTGCAAGCTCTGGTGACTACAACAAAAACCAGTACTACGGCAt                                                                                    <  3:13348/149‑1 (MQ=255)                                                                           ccTGAAATACCGCTATGAAGAAGACAACAGCCCGCTGGGTGTGATCGGTTCTTTCACTGACACCGAGAAAAGCCGTACTGCAAGCTCTGGTGACTACAACAAAAACCAGTACTACGGCATCACTGCTGGTCCGGCTTACCGCATTAACGAc                                                     <  1:273566/151‑1 (MQ=255)                                                                                          tGAAGAAGACAACAGCCCGCTGGGTGTGATCGGTTCTTTCACTGACACCGAGAAAAGCCGTACTGCAAGCTCTGGTGACTACAACAAAAACCAGTACTACGGCATCACTGCTGGTCCGGCTTACCGCATTAACGACTGGGCAAGCATCTAc                                      <  1:253550/151‑1 (MQ=255)                                                                                          tGAAGAAGACAACAGCCCGCTGGGTGTGATCGGTTCTTTCACTGACACCGAGAAAAGCCGTACTGCAAGCTCTGGTGACTACAACAAAAACCAGTACTACGGCATCACTGCTGGTCCGGCTTACCGCATTAACGACTGGGCAAGCATCTAc                                      <  1:42387/151‑1 (MQ=255)                                                                                          tGAAGAAGACAACAGCCCGCTGGGTGTGATCGGTTCTTTCACTGACACCGAGAAAAGCCGTACTGCAAGCTCTGGTGACTACAACAAAAACCAGTACTACGGCATCACTGCTGGTCCGGCTTACCGCATTAACGACTGGGCAAGCATCTAc                                      <  1:205747/151‑1 (MQ=255)                                                                                           gaagaaGACAACAGCCCGCTGGGTGTGATCGGTTCTTTCACTGACACCGAGAAAAGCCGTACTGCAAGCTCTGGTGACTACAACAAAAACCAGTACTACGGCATCACTGCTGGTCCGGCTTACCGCATTAACGACTGGGCAAGCATCTACg                                     >  1:278593/1‑151 (MQ=255)                                                                                           gaagaaGACAACAGCCCGCTGGGTGTGATCGGTTCTTTCACTGACACCGAGAAAAGCCGTACTGCAAGCTCTGGTGACTACAACAAAAACCAGTACTACGGCATCACTGCTGGTCCGGCTTACCGCATTAACGACTGGGCAAGCATCTACg                                     >  3:279087/1‑151 (MQ=255)                                                                                            aagaagACAACAGCCCGCTGGGTGTGATCGGTTCTTTCACTGACACCGAGAAAAGCCGTACTGCAAGCTCTGGTGACTACAACAAAAACCAGTACTACGGCATCACTGCTGGTCCGGCTTACCGCATTAACGACTGGGCAAGCATCTACgg                                    <  3:410745/151‑1 (MQ=255)                                                                                                    aacaGCCCGCTGGGTGTGATCGGTTCTTTCACTGACACCGAGAAAAGCCGTACTGCAAGCTCTGGTGACTACAACAAAAACCAGTACTACGGCATCACTGCTGGTCCGGCTTACCGCATTAACGACTGGGCAAGCATCTACGGTGTAgtgg                            <  3:327053/151‑1 (MQ=255)                                                                                                      caGCCCGCTGGGTGTGATCGGTTCTTTCACTGACACCGAGAAAAGCCGTACTGCAAGCTCTGGTGACTACAACAAAAACCAGTACTACGGCATCACTGCTGGTCCGGCTTACCGCATTAACGACTGGGCAAGCATCTACGGTGTAgtgggt                          >  1:466916/1‑151 (MQ=255)                                                                                                         cccGCTGGGTGTGATCGGTTCTTTCACTGACACCGAGAAAAGCCGTACTGCAAGCTCTGGTGACTACAACAAAAACCAGTACTACGGCATCACTGCTGGTCCGGCTTACCGCATTAACGACTGGGCAAGCATCTACGGTGTAgtgggtg                         <  3:259353/149‑1 (MQ=255)                                                                                                         cccGCTGGGTGTGATCGGTTCTTTCACTGACACCGAGAAAAGCCGTACTGCAAGCTCTGGTGACTACAACAAAAACCAGTACTACGGCATCACTGCTGGTCCGGCTTACCGCATTAACGACTGGGCAAGCATCTACGGTGTAgtgggt                          <  3:158300/148‑1 (MQ=255)                                                                                                         cccGCTGGGTGTGATCGGTTCTTTCACTGACACCGAGAAAAGCCGTACTGCAAGCTCTGGTGACTACAACAAAAACCAGTACTACGGCATCACTGCTGGTCCGGCTTACCGCATTAACGACTGGGCAAGCATCTACGGTGTAgtggg                           <  1:260864/147‑1 (MQ=255)                                                                                                               gggTGTGATCGGTTCTTTCACTGACACCGAGAAAAGCCGTACTGCAAGCTCTGGTGACTACAACAAAAACCAGTACTACGGCATCACTGCTGGTCCGGCTTACCGCATTAACGACTGGGCAAGCATCTACGGTGTAGTGGGTGTGGGTTAt                 >  3:112821/1‑151 (MQ=255)                                                                                                                 gtgtGATCGGTTCTTTCACTGACACCGAGAAAAGCCGTACTGCAAGCTCTGGTGACTACAACAAAAACCAGTACTACGGCATCACTGCTGGTCCGGCTTACCGCATTAACGACTGGGCAAGCATCTACGGTGTAGTGGGTGTGGGTTATgg               >  3:132887/1‑151 (MQ=255)                                                                                                                      aTCGGTTCTTTCACTGACACCGAGAAAAGCCGTACTGCAAGCTCTGGTGACTACAACAAAAACCAGTACTACGGCATCACTGCTGGTCCGGCTTACCGCATTAACGACTGGGCAAGCATCTACGGTGTAGTGGGTGTGGGTTATGGTAAAt          <  3:311987/151‑1 (MQ=255)                                                                                                                          gTTCTTTCACTGACACCGAGAAAAGCCGTACTGCAAGCTCTGGTGACTACAACAAAAACCAGTACTACGGCATCACTGCTGGTCCGGCTTACCGCATTAACGACTGGGCAAGCATCTACGGTGTAGTGGGTGTGGGTTATGGTAAATTcc       <  3:362371/150‑1 (MQ=255)                                                                                                                             ccttCACTGACACCGAGAAAAGCCTTACTGCAAGCTCTGGAGACTACAACAAAAACCAGTACTACTTCATCACTGCTGGTCATACTTACTGCATCAACGACTGGGCAAGCATCTACGGTGTAGTGGGGGTGGGTTATGGTAAATTACAGat   >  3:86744/3‑150 (MQ=255)                                                                                                                              tttCACTGACACCGAGAAAAGCCGTACTGCAAGCTCTGGTGACTACAACAAAAACCAGTACTACGGCATCACTGCTGGTCCGGCTTACCGCATTAACGACTGGGCAAGCATCTACGGTGTAGTGGGTGTGGGTTATGGTAAATTCCAGAcc  <  3:150097/151‑1 (MQ=255)                                                                                                                                     |                                                                                                                                                GCGACTTCTACTGTAACTGGCGGTTACGCACAGAGCGACGCTCAGGGCCAAATGAACAAAATGGGCGGTTTCAACCTGAAATACCGCTATGAAGAAGACAACAGCCCGCTGGGTGTGATCGGTTCTTTCACTTACACCGAGAAAAGCCGTACTGCAAGCTCTGGTGACTACAACAAAAACCAGTACTACGGCATCACTGCTGGTCCGGCTTACCGCATTAACGACTGGGCAAGCATCTACGGTGTAGTGGGTGTGGGTTATGGTAAATTCCAGACC  >  NC_000913/850519‑850794

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑