Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,955,324	(G)6→7	coding (1847/1851 nt)	ilvD →	dihydroxyacid dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,955,318	1	.	G	100.0%	59.1 / NA	18	L614R (CTG→CGG)	ilvD	dihydroxyacid dehydratase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (7/11);  total (7/11)

GCGTCGTGAAGCGCAGGACGCTCGAGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACT‑GGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACAAAA  >  NC_000913/3955178‑3955437                                                                                                                                              |                                                                                                                        gcgTCGTGAAGCGCAGGACGCTCGAGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACT‑gggggggtta                                                                                                               >  2:302069/1‑147 (MQ=255)     cgtGAAGCGCAGGACGCTCGAGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGGTtaataa                                                                                                            <  2:141633/151‑1 (MQ=255)                 gACGCTCGAGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGGTTAATAATGGCTGACt                                                                                                   <  1:276681/148‑1 (MQ=255)                     cTCGAGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGGTTAATAATGGCTGACTCGCAAcc                                                                                            <  1:69083/151‑1 (MQ=255)                       cGAGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGGTTAATAATGGCTGACTCGCAAccc                                                                                           >  2:40599/1‑150 (MQ=255)                            tGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTcc                                                                                     <  1:149685/151‑1 (MQ=255)                                         aCGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGaa                                                                         <  1:106375/150‑1 (MQ=255)                                                   aTCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAata                                                              >  2:141693/1‑151 (MQ=255)                                                         aaCGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAag                                                        >  2:177145/1‑151 (MQ=255)                                                         aaCGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAag                                                        >  2:164813/1‑151 (MQ=255)                                                            gTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGGTTAATAATGGCTGACTCGCAACCACTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAagag                                                      >  1:177048/1‑150 (MQ=255)                                                             tCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGc                                                     <  2:315085/150‑1 (MQ=255)                                                             tCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGc                                                     <  1:302071/150‑1 (MQ=255)                                                              cAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAg                                                   >  1:55089/1‑151 (MQ=255)                                                                  tctcCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGGTTAGTAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGtgc                                                <  1:105660/150‑1 (MQ=255)                                                                                   gCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACg                              >  1:88817/1‑151 (MQ=255)                                                                                                  gCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGtt                <  2:308098/150‑1 (MQ=255)                                                                                                  gCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGtt                <  1:164010/150‑1 (MQ=255)                                                                                                               aCAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACaaaa  <  1:75855/151‑1 (MQ=255)                                                                                                                                              |                                                                                                                        GCGTCGTGAAGCGCAGGACGCTCGAGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACT‑GGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACAAAA  >  NC_000913/3955178‑3955437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑