New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 3927418 =20 (0.980)3 (0.160) 3/274 2.0 15.6% coding (264/993 nt) asnA asparagine synthetase A
?NC_000913 3927457 = 14 (0.750)coding (303/993 nt) asnA asparagine synthetase A
Rejected: Frequency below cutoff threshold.

CAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTA  >  NC_000913/3927320‑3927587
                                                                                                                                          |                                                                                                                                  
cAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGt                                                                                                                        >  2:185018/1‑150 (MQ=255)
    agtcgagaattgacgctcaccgtcgcccattacgcgttcccagtcccactggtcaacatagaccgagtgcaacggagaaagacggtcttcatcgGGGCGAAGGGCTTTCATGTGCGTGTACAGCCCTTCGCCCCGATGAAGACCGTCt                                                                                                                      >  1:42190‑M1/95‑148 (MQ=255)
      gtcgagaattgacgctcaccgtcgcccattacgcgttcccagtcccactggtcaacatagaccgagtgcaacggagaaagacggtcttcatcggggcgaagggctgtacacgcacatgaaagcccttcgcccCGATGAAGACCGTCttt                                                                                                                    >  2:171823‑M2/133‑149 (MQ=255)
      aaaGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTtctc                                                                                                                 <  2:243153/151‑1 (MQ=255)
            aaaGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTg                                                                                                            <  2:140182/150‑1 (MQ=255)
                    gCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGt                                                                                                    >  1:251687/1‑150 (MQ=255)
                      cTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTa                                                                                                 >  1:249081/1‑151 (MQ=255)
                         aTGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAGGACCGTCTTTCTCCGTTGCACTCGGTCTATGt                                                                                              <  1:304074/151‑1 (MQ=255)
                         aTGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGt                                                                                              <  2:21038/151‑1 (MQ=255)
                          tcgcccattacgcgttcccagtcccactggtcaacatagaccgagtgcaacggagaaagacggtcttcatcgGGGCGAAGGGCTTTCATGTGCGTGTACAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGt                                                                                              >  2:202033‑M1/73‑150 (MQ=255)
                                gTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAg                                                                                       >  2:85354/1‑151 (MQ=255)
                                     aaGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGa                                                                                  >  1:25454/1‑151 (MQ=255)
                                            tcattcaCTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGaa                                                                           <  2:129929/151‑1 (MQ=255)
                                                            acatagaccgagtgcaacggagaaagacggtcttcatcggggcgaagggctgtacacgcacatgaaagcccttcgcccCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGa                                                             >  2:202033‑M2/79‑150 (MQ=255)
                                                               aaaCGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTg                                                        <  2:222742/151‑1 (MQ=255)
                                                                                 aaagacggtcttcatcgGGGCGAAGGGCTTTCATGTGCGTGTACAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGAc                                        >  2:171823‑M1/18‑149 (MQ=255)
                                                                                    agacggtcttcatcggggcgaagggctgtacacgcacatgaaagcccttcgcccCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGAct                                       >  1:42190‑M2/55‑148 (MQ=255)
                                                                                      cGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGaaa                                 <  2:96633/151‑1 (MQ=255)
                                                                                                    GCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAgg                    <  2:177905‑M1/150‑1 (MQ=255)
                                                                                                           GCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTgg            <  1:311153‑M1/151‑1 (MQ=255)
                                                                                                                   cGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAAt    >  2:216545/1‑151 (MQ=255)
                                                                                                                     cacaTGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTa  >  2:158588/1‑151 (MQ=255)
                                                                                                                                          |                                                                                                                                  
CAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTA  >  NC_000913/3927320‑3927587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.