| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,037,130 | 0 | T | A | 63.6% | 1.3 / 10.2 | 13 | L128M (TTG→ATG) | hyaF | hydrogenase‑1 protein nickel incorporation factor |
| Reads supporting (aligned to +/- strand): ref base T (2/2); new base A (7/0); total (11/2) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.09e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.15e-03 | |||||||||||
ATCAATACGCTTCTCGGGGAAGGGGAAGTGTCAGTGCGCATTCAGCAGGCTGACGACAGTGAAAGTGAAATACAGGAGGCGATCTTCTGCGGATTATGGCGGGTGCGCAGACGTCGCGGCGAAAAGTTGCTGGAGGACAAACTGGAGGCTGGCTGCGCGCCGCTGGCGTTGTGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCCCATTGATGGCCTGATGAATGGCCTACCGTTGGCGCATGAG > NC_000913/1037004‑1037267 | aTCAATACGCTTCTCGGGGAAGGGGAAGTGTCAGTGCGCATTCAGCAGGCTGACGACAGTGAAAGTGAAATACAGGAGGCGATCTTCTGCGGATTATGGCGGGGGGGCCGACGGCGCGGCGGAAAAATGTTGGGGGGCAAAAaggaggggg > 1:114087/1‑148 (MQ=255) gggaaggggaagTGTCAGTGCGCATTCAGCAGGCTGACGACAGTGAAAGTGAAATACAGGAGGCGATCTTCTGCGGATTATGGCGGGGGGGCCGACGGCGCGGCGGAAAAATGTTGGGGGGGAAACTGGAGGGGGGGGGCGcgccccggg > 1:135722/1‑145 (MQ=255) gggaagTGTCAGTGCGCATTCAGCAGGCTGACGACAGTGAAAGTGAAATACAGGAGGCGATCTTCTGCGGATTATGGCGGGGGGGGGGACGGCCCGGCGGGAAAGTGCTGGGGGGAAAACCGGGGGGTGGCCGGGCGCCCCCGGCGGtgtg > 2:241453/1‑151 (MQ=255) gtgtCAGTGCGCATTCAGCAGGCTGACGACAGTGAAAGTGAAATACAGGAGGCGATCTTCTGCGGATTATGGCGGGGGGGCGGACGGCGCGGGGGAAAAATGGTGGGGGGAAAAATGGAGGGGGGGCGCGCGCCGCCGGCGGTgggggggg > 1:166696/1‑147 (MQ=255) gtgtCAGTGCGCATTCAGCAGGCTGACGACAGTGAAAGTGAAATACAGGAGGCGATCTTCTGCGGATTATGGCGGGGGGGCGGAAGGCGCGGGGGAAAAATGCTGGGGGGGAAAATGGAGGGTGGGTGCGCGCCGCCGGCGGTGTgggggg > 1:96999/1‑147 (MQ=255) cAGTGCGCATTCAGCAGGCTGACGACAGTGAAAGTGAAATACAGGAGGCGATCTTCTGCGGATTATGGCGGGGGGGCCGAAGGCGCGGCGGAAAAATGCTGGGGGGCAAAAAGGAGGGTGGCTGCGCGCCCCCGGCGGTGTGGGAggcggg > 1:223492/1‑150 (MQ=255) ttCAGCAGGCTGACGACAGTGAAAGTGAAATACAGGAGGCGATCTTCTGCGGATTATGGCGGGTGCGCAGACGGCGCGGGGGAAAAGTGGTGGGGGGAAAACTGGAGGGTGGCTGGGCGCCGCCGGGGGTGTGGGAggggggcaagcacaa > 1:115881/1‑141 (MQ=255) gaaaTACAGGAGGCGATCTTCTGCGGATTATGGCGGGGGGGGCGAAGGCGCGGCGGAAAAATGCTGGGGGGGAAAATGGGGGGTGGCTGCGCGCCGCCGGCGGTGTGGGAGGGGGGGACGCAAAAAATCTTTCCGCCAGATTTGCttttt > 1:23740/1‑149 (MQ=255) gaaaTACAGGAGGCGATCTTCTGCGGATTATGGCGGGGGGGCCGACGGCGCGGCGGAAAGATGCTGGGGGGCAAAATGGAGGCTGGCCGCGCGCCGCCGGCGGTGGGGGGGGGGGGCACGCCAAAAATCTTTCCCGCAGATTCTCtttttt > 1:261120/1‑149 (MQ=255) aTGGCGGGTGCGCAGACGTCGCGGCGAAAAGTTGCTGGAGGACAAACTGGAGGCTGGCTGCGCGCCGCTGGCGTTGTGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCCCATTGATGGCCTGATGAATgg > 1:171393/1‑150 (MQ=255) aTGGCGGGTGCGCAGACGTCGCGGCGAAAAGTTGCTGGAGGACAAACTGGAGGCTGGCTGCGCGCCGCTGGCGTTGTGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCCCATTGATGGCCTGATGAATgg > 1:200275/1‑150 (MQ=255) aCGTCGCGGCGAAAAGTTGCTGGAGGACAAACTGGAGGCTGGCTGCGCGCCGCTGGCGTTGTGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCCCATTGATGGCCTGATGAATGGCCTACCGTTGgcg < 2:217092/148‑1 (MQ=255) tCGCGGCGAAAAGTTGCTGGAGGACAAACTGGAGGCTGGCTGCGCGCCGCTGGCGTTGTGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCCCATTGATGGCCTGATGAATGGCCTACCGTTGGCGCATGAg < 1:241455/151‑1 (MQ=255) | ATCAATACGCTTCTCGGGGAAGGGGAAGTGTCAGTGCGCATTCAGCAGGCTGACGACAGTGAAAGTGAAATACAGGAGGCGATCTTCTGCGGATTATGGCGGGTGCGCAGACGTCGCGGCGAAAAGTTGCTGGAGGACAAACTGGAGGCTGGCTGCGCGCCGCTGGCGTTGTGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCCCATTGATGGCCTGATGAATGGCCTACCGTTGGCGCATGAG > NC_000913/1037004‑1037267 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |