| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,622,816 | 0 | G | A | 57.1% | ‑6.3 / 12.6 | 14 | intergenic (+75/+144) | yneM/dgcZ | inner membrane‑associated protein/diguanylate cyclase, zinc‑sensing |
| Reads supporting (aligned to +/- strand): ref base G (6/0); new base A (0/8); total (6/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.33e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.05e-03 | |||||||||||
GTACTGGGAATAATTTTATTTTCTGGTTTTCTGGCCGCGTATTTCAGCCACAAATGGGATGACTAA‑TGAACGGAGATAATCCCTCACCTAACCGGCCCCTTGTTACAGTTGTG‑TACAAGGGGCCTGATTTTTATGACGGCGAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGTTTTCTCAATGAATGTTAAACGG > NC_000913/1622676‑1622956 | gTACTGGGAATAATTTTATTTTCTGGTTTTCTGGCCGCGTATTTCAGCCACAAATGGGATGACTAA‑TGAACGGAGATAATCCCTCACCTAACCGGCCCCTTGTTACAGTTGTG‑TACAAGGGGCCTGATTTTTATGACGGCGAAAAAAAAc > 2:34246/1‑150 (MQ=255) cccGCTTATTTCACCCAAAAGGGGGTTGACAAA‑TGAAGGGAAATAACCCCTCCCCTAACCGCCCCTTTTTTACATTTGTG‑TAAAGGGGGCTTGTTTTTTATGACGGAAAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGAt < 2:98429/150‑1 (MQ=255) tttttccAGCCAAAAAGGGGATGACTAA‑TGAACGGAAATAATCCCCCCCCTACCCGCCCCCTTGTTACAGTTGTG‑TACAGGGGGCCTATTTTTTAGGAGGGAAAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGAtt < 1:109813/145‑1 (MQ=255) tttttCAGCCA‑AAAGGGGAAATCTAATTGAAGGGATATAATCCCTC‑CCCAACCCGCCCCCTTTTGGAGGTGTGTTACAGGGGGCTTTATTTTTGTGAGGGAAAAAAAAAGCCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGAtt < 2:164853/148‑1 (MQ=255) ggATGATTAA‑TGAAGGGAGATAACCCCCCCCCAACCCGGCCCCTTTTTACATTTGTG‑AACAGGGGGCCTGTTTTTTATGAGGGAAAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACg < 1:239872/150‑1 (MQ=255) cTAA‑TGAACGGAGATAATCCCTCACCTAACCGGCCCCTTGTTACAGTTGTG‑TACAAGGGGCCTGATTTTTATGACGGCGAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAg > 1:270020/1‑151 (MQ=255) gaTAATCCCTCACCTAACCGGCCCCTTGTTACAGTTGTG‑TACAAGGGGCCTGATTTTTATGACGGCGAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTg > 1:68762/1‑150 (MQ=255) aaaccccTCCCCTACCCGCCCCCTTTTAACTGTTGTT‑TACAGGGGGCTTGTTTTTTATGACGGAAAAAAAAACCCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCAc < 2:90260/147‑1 (MQ=255) tCCCTCACCTAACCGGCCCCTTGTTACAGTTGTG‑TACAAGGGGCCTGATTTTTATGACGGCGAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGa > 2:41467/1‑151 (MQ=255) caacccGCCCCTTTGTTACATTTGTG‑AAAAGGGGGCTTTTTTTTAAGGCCGGAAAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAAt < 2:46493/147‑1 (MQ=255) cccTTGTTACAGTGGTG‑TAAAGGGGGCTTGTTTTTTATGACGGAAAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGacac < 2:68762/151‑1 (MQ=255) gTTGTG‑TACAAGGGGCCTGATTTTTATGACGGCGAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAg > 2:283217/1‑151 (MQ=255) gTTGTG‑TACAAGGGGCCTGATTTTTATGACGGCGAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAg > 2:30160/1‑151 (MQ=255) ttATGACGGAAAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGTTTTCTCAATGAATGTTAAACgg < 2:169867/151‑1 (MQ=255) | GTACTGGGAATAATTTTATTTTCTGGTTTTCTGGCCGCGTATTTCAGCCACAAATGGGATGACTAA‑TGAACGGAGATAATCCCTCACCTAACCGGCCCCTTGTTACAGTTGTG‑TACAAGGGGCCTGATTTTTATGACGGCGAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGTTTTCTCAATGAATGTTAAACGG > NC_000913/1622676‑1622956 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |