Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,556,2070TC59.3% 6.1 / 28.2 27S215G (AGT→GGT) rtcARNA 3'‑terminal phosphate cyclase
Reads supporting (aligned to +/- strand):  ref base T (9/2);  new base C (0/16);  total (9/18)
Fisher's exact test for biased strand distribution p-value = 1.17e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.62e-04

GCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGTTGCCACCACA  >  NC_000913/3556059‑3556355
                                                                                                                                                    |                                                                                                                                                    
gCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTc                                                                                                                                                     >  2:99313/1‑150 (MQ=255)
                          cTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCa                                                                                                                          >  2:315598/1‑151 (MQ=255)
                            tttCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAg                                                                                                                         >  2:5929/1‑150 (MQ=255)
                             tcccccaccaaaaaaagaGCTTTCGTTGATATTTTCCCTGTCCATTTCATGCAAGACTTTATACCCCGCCCCCGGGTCGGGCGGCAGTTTATGAATATTCTGTTAATACAGGAAAAACCCCGCCGCCAGTGTAGCGATTTCACGCTCAGCa                                                                                                                       <  2:14373/139‑1 (MQ=255)
                               ccccGACGACAAAAAAGCGTTCGGTGATATTTTCCCTTTCGACTTCAAGCGAGACGGTATTCCCCGGCCCCTGTTCGCGCGGCAGTTTATGAATATTCTGTTCATGCAGGAAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAAc                                                                                                                     <  1:172332/149‑1 (MQ=255)
                                 ccGACGAAAAAAAAGCGTTCGGTGATATTTTCCCTTTCGACTTCAAGCGAGACGTTATTCCCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACAt                                                                                                                   <  1:257695/151‑1 (MQ=255)
                                 ccGACAAAAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATCCCCCGGCCCCGGGTCGCGCGGCAGTTTATGAATATTCTGTTCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACAt                                                                                                                   <  2:201690/151‑1 (MQ=255)
                                                       tGATATTTTCCCTTTCGACTTCAAGCGAGACGGTATTCCCCGGCCCCTGGTCGCGGGGCAGTTTATGAATTTTCTGTTCATGCAGGAAAAAACCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAg                                                                                             <  1:37385/151‑1 (MQ=255)
                                                       tGATATTTTCCCTTCCGACTTAAAGCGAGACGGTATTCCCCGGCCCCGGGGCGCGCGGCAGGTTATAAATATTCTGTTCATGGAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAg                                                                                             <  2:279514/151‑1 (MQ=255)
                                                       tGATATTTTCCCTTACGACTTAAAGAGAGACGGTATCCCCCGCCCACTGCCCGCGGGCCAGTTTATGAATTTTCTGTTCAGGAAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAg                                                                                             <  2:178122/151‑1 (MQ=255)
                                                          tatTTTCCCTTCCGACTTCAAGCGAGACGGTATTCCCCGGCCCCGGGTCGCGCGGCAGTTTATAAATTTTCTGTTCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAAc                                                                                          <  2:153628/151‑1 (MQ=255)
                                                          tatTTCCACTTTCGACTTCAAGCGAGCCGGTATTCCCCGCCCCCTGGCCGCGCGGCAGTTTATGAATTTTCTGTTCAGGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAAc                                                                                          <  2:183148/151‑1 (MQ=255)
                                                                     ccgccTTCAAGCGAGACGGTATTCCCCGCCCCCTGGTCGCGCGGCAGGTTATGAATTTTCTGTTCATGCAGGGAAAAACCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCa                                                                               <  2:312995/147‑1 (MQ=255)
                                                                               gaggtaCGGTATTCCCCGCCCCCTGGCCGCGCGGAAGTTAAGAAATTTTCTGCTCATGAAGGGAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACaa                                                                      <  2:273635/145‑1 (MQ=255)
                                                                                cGAGAGGGTATTCCCCGGCCCCGGGTCGCGCGGCAGTTTATGAATATTCTGTTAATGCAGGGAAAAACCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACaa                                                                      <  1:257611/149‑1 (MQ=255)
                                                                                 gagaCGGTATTCCCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTTTTCATGCAGGGAAAAACCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATg                                                                    <  1:310896/150‑1 (MQ=255)
                                                                                  agaCGTTATTCCCCGCCCCTTGGTCGCGCGGCAGTTTATGAATATTCTGTTCATGCAGGGAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGt                                                                   <  2:299904/150‑1 (MQ=255)
                                                                                       gTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCcg                                                              >  1:294540/1‑150 (MQ=255)
                                                                                       gTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCcg                                                              >  2:316598/1‑150 (MQ=255)
                                                                                       gTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCcg                                                              >  2:4883/1‑150 (MQ=255)
                                                                                         tttccccGCCCCCTGGTCGCGCGGCAGTTTATGAATTTTCTGTTCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCgcgc                                                           <  1:192054/147‑1 (MQ=255)
                                                                                                                    tttATGAATATTCTGTTCATGCAGGAAAAAACCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAAc                                   <  2:47404/147‑1 (MQ=255)
                                                                                                                    gTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGAt                                >  2:42560/1‑151 (MQ=255)
                                                                                                                    gTTATGAATATTCTGTCCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAAc                                   <  2:71880/148‑1 (MQ=255)
                                                                                                                    gTTATAAATATTCTGTTCATGCAGGAAAAACCTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAAc                                   <  1:248353/148‑1 (MQ=255)
                                                                                                                                                aaaCTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGTTGccacca    >  1:220656/1‑151 (MQ=255)
                                                                                                                                                  aCTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCGAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGTTGCCACcaca  >  1:232278/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGTTGCCACCACA  >  NC_000913/3556059‑3556355

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑