| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,637,360 | 0 | G | A | 57.1% | 0.9 / 11.6 | 14 | F17F (TTC→TTT) | yhiN | putative oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base G (4/2); new base A (0/8); total (4/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.50e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.05e-03 | |||||||||||
GGCTCAGATAAGCGCCTGGTTCGACATAAAGG‑TTGGTAAA‑GTTG‑CAG‑CGCCCACCGCCAGACATAAGGATTTTGCGCCCTGGTTTTTT‑A‑CCA‑TTATCGATCAGCAGAACCCGGCGTCCTGCCTGACCTGCCAGCGCAGAACAGAACATACCCGCCGCACCAGCGCCTATAATAATGGCATCAAACCTTTCCACGTTGCGCTCCTCTTAGAAAAAACGGGCGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATA > NC_000913/3637218‑3637510 | ggCTCAGATAAGCGCCTGGTTCGACATAAAGG‑TTGGTAAA‑GTTG‑CAG‑CGCCCACCGCCAGACATAAGGATTTTGCGCCCTGGTTTTTT‑A‑CCA‑TTATCGATCAGCAGAACCCGGCGTCCTGCCTGACCTGCCAGCGCAGAACAGAACATAc > 1:257599/1‑150 (MQ=255) caaaaaaaGG‑GTGGTAAA‑GTTGCCAC‑CCCCCCCCGCCAGA‑AAAAGGTTTTTGCCCCCTTGTTTTTTCC‑CCA‑TTCTCAACCAGCAAACCCGGGCCCCCGGCCTCCCCGCCCAGCGAAAAAAAAAACACCCCCGCCGCACCAGCGCCTataat < 2:37182/146‑1 (MQ=255) aCAAAAAGG‑TGGTAAAA‑GTCG‑CCG‑CCCCCCCCGCAAGAAATAAGGTTTTTGCGCCCTGGTTTTTT‑A‑CCT‑TTATCGATAAGAAGACCCCGGCGTCCGGCCTGCCCTGCCAGCGAAGAAAAAAACACCCCCGCCGCACCAGCGCCTAtaataa < 1:246248/151‑1 (MQ=255) caaaaaGG‑GTGGAAAA‑GTTT‑CCGCCCCCCCCCCCCAGCAATAAGGTTTTGCCGCCGGGGTTTTTT‑C‑CCA‑TCACAGCCCAGCAGACCCCGCCGCCCTGCCTGAACTACCAGCACAGAACAGACCATCCCCGCCGCACCAGCGCCTAtaataa < 2:76056/148‑1 (MQ=255) aaaaGGTTTGGAAAA‑TTTG‑CAG‑CCCCCCCCCCCAGACAAAAGTTTTTTGCCCCCTGTTTTTTT‑C‑CTT‑TTATCGACCAACAAACCCCGGCGCCCTCCCTGCCCTGCCAGCGAAAAACAAAACACCCCCGCCGCACCAGCGCCTATAATAATg < 1:48140/150‑1 (MQ=255) aaaaGG‑GTGGGAAAGGTTG‑CAG‑CCCCCACCGCCAGAAAAAAGGTTTTTGC‑CCCTTGTTTTTT‑T‑CCATTTATCGACAAGCAAACCCCGGCTCCCTGCCTGACCTGCCAGGGAAAAACAGAACATACCCGCCGCACCAGCGCCTATAATAATg < 1:305076/150‑1 (MQ=255) aaaaaaaGGTTTTTGCGCCTTGTTTTTTT‑A‑CCA‑TTACCAACCACCAGCCCCGGGCTCCCTGCCGCCCCGGCCAGCGCAAAACAAAACATCCCCGCCGCACCAGCGCCTATAATAATGGCATCAAACCTTTCCACGTTGCGCTCCTCTTAGa < 2:33684/146‑1 (MQ=255) cATAAGGATTTTGCGCCCTGGTTTTTT‑A‑CCA‑TTATCGATCAGCAGAACCCGGCGTCCTGCCTGACCTGCCAGCGCAGAACAGAACATACCCGCCGCACCAGCGCCTATAATAATGGCATCAAACCTTTCCACGTTGCGCTCCTCTTAGaaa > 1:96041/1‑151 (MQ=255) ttttttt‑ACCCT‑TTACCGACAACCAGACCCGGGCTCCCTGCCTGACCTCCCAGCGCAAAAAAAAACATCCCCGCCGCACCAGCGCCTATAATAATGGCATCAAACCTTTCCACGTTGCGCTCCTCTTAGAAAAAACGGGCGTGAATTGTaa < 1:189124/150‑1 (MQ=255) a‑TTATCGACCAGCAGACCCCGGCTTCCGGCCTGCCCTCCCAGCGAAAAACAAAACATCCCCGCCGCACCAGCGCCTATAATAATGGCATCAAACCTTTCCACGTTGCGCTCCTCTTAGAAAAAACGGGCGTGAATTGTAAAGATTCCTCAg < 1:255558/151‑1 (MQ=255) tCGATCAGCAGAACCCGGCGTCCTGCCTGACCTGCCAGCGCAGAACAGAACATACCCGCCGCACCAGCGCCTATAATAATGGCATCAAACCTTTCCACGTTGCGCTCCTCTTAGAAAAAACGGGCGTGAATTGTAAAGATTCCTCAGTGGt > 2:235148/1‑151 (MQ=255) ctccctgcctgCCCGCCCAGCGCAAAAAAAACCATCCCCGCCGCACCAGCGCCTATAATAATGGCATCAAACCTTTCCACGTTGCGCTCCTCTTAGAAAAAACGGGCGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAatat < 1:29942/147‑1 (MQ=255) gcgcAGAACAGAACATACCCGCCGCACCAGCGCCTATAATAATGGCATCAAACCTTTCCACGTTg > 1:225269/1‑65 (MQ=255) cgcAGAAAAAAACACCCCCGCCGCACCAGCGCCTATAATAATGGCATCAAACCTTTCCACGTTGCGCTCCTCTTAGAAAAAACGGGCGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGcc < 2:274603/151‑1 (MQ=255) gaacagaacaTACCCGCCGCACCAGCGCCTATAATAATGGCATCAAACCTTTCCACGTTGCGCTCCTCTTAGAAAAAACGGGCGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGaa > 2:189570/1‑151 (MQ=255) gaacaTACCCGCCGCACCAGCGCCTATAATAATGGCATCAAACCTTTCCACGTTGCGCTCCTCTTAGAAAAAACGGGCGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATtata > 2:73183/1‑151 (MQ=255) | GGCTCAGATAAGCGCCTGGTTCGACATAAAGG‑TTGGTAAA‑GTTG‑CAG‑CGCCCACCGCCAGACATAAGGATTTTGCGCCCTGGTTTTTT‑A‑CCA‑TTATCGATCAGCAGAACCCGGCGTCCTGCCTGACCTGCCAGCGCAGAACAGAACATACCCGCCGCACCAGCGCCTATAATAATGGCATCAAACCTTTCCACGTTGCGCTCCTCTTAGAAAAAACGGGCGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATA > NC_000913/3637218‑3637510 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |