Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,279,468	0	C	G	55.6%	14.9 / 26.3	18	G330G (GGC→GGG)	ghxP	guanine/hypoxanthine permease, high affinity; guanine/hypoxanthine:H+ symporter
Reads supporting (aligned to +/- strand):  ref base C (2/6);  new base G (10/0);  total (12/6)
Fisher's exact test for biased strand distribution p-value = 1.51e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.11e-03

GGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTACGGCGGCGGGCGGTAAAACCGGTTTGACGGCTATCACCGTTGGCGTGCTGTTCCTCCTGATTCTGTTCCTCTCTCCGCTCTCTTACCTCGTTCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGA  >  NC_000913/4279324‑4279613                                                                                                                                                 |                                                                                                                                                  ggCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTACGGCGGGGGGGGGTaaa                                                                                                                                             >  1:92899/1‑151 (MQ=255)          gctgGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGGACGGCGGGGGGGGGGAAAAACGGgttt                                                                                                                                    >  1:168796/1‑150 (MQ=255)             ggATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGGACGGCGGGGGGGGGGGAAACCCGGTTGGCg                                                                                                                                 >  2:206112/1‑151 (MQ=255)               aTAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTACGGCGGCGGGCGGTAAAACCGGTTTGACgg                                                                                                                                <  2:168795/150‑1 (MQ=255)                                atcGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTACGGCGGCGGGCGGTAAAACCGGTTTGACGGCTATCACCGTTGGCGTGc                                                                                                              <  2:147147/151‑1 (MQ=255)                                         gggAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTACGGCGGCGGGCGGGAAAACCGGGTTGACGGCTCTCACCGTTTGGGTGCTGTtcctc                                                                                                      >  1:232943/1‑150 (MQ=255)                                          ggAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTACGGCGGCGGGGGGTAAAACCGGGTTGACGGCTATCACCGCTGGCGGGCCGTtcctcc                                                                                                     >  2:123308/1‑150 (MQ=255)                                                             cTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGGACGGCGGCGGGGGGGAAAAACGGGTTGACGGCTATCACCGCTGGGGGGCTGTTTCCCCTGATTCTGTTTCTCTCTcc                                                                                  >  1:137697/1‑150 (MQ=255)                                                                               ctctGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTACGGCGGGGGGGGGGAAAAACGGGTTGACGGCTATCACCGTTGGCGGGCTGTTCCTCCCTATTCTGTTCCCCTCTCCGCCCTCTTTCCTCGtttc                                                                >  1:32248/1‑148 (MQ=255)                                                                                  tGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTACGGCGGCGGGCGGTAAAACCGGTTTGACGGCTATCACCGTTGGCGTGCTGTTCCTCCTGATTCTGTTCCTCTCTCCGCTCTCTTACCTCGTTCCgggg                                                            <  1:228464/151‑1 (MQ=255)                                                                                   ggCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGGACGGCGGGGGGGGGGAAAACCGGGTTTACGGGTATTACCGGTTGGGGGCTGGTTCTCCCGATTTTGTTTCTCTCTCCCCTCTCTTTCCTCGTTTCggggg                                                           >  1:56207/1‑150 (MQ=255)                                                                                    gCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTACGGCGGCGGGCGGTAAAACCGGTTTGACGGCTATCACCGTTGGCGTGCTGTTCCTCCTGATTCTGTTCCTCTCTCCGCTCTCTTACCTCGTTCCGGGGt                                                           <  2:231315/150‑1 (MQ=255)                                                                                          tgggtgCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTACGGCGGCGGGCGGTAAAACCGGTTTGACGGCTATCACCGTTGGCGTGCTGTTCCTCCTGATTCTGTTCCTCTCTCCGCTCTCTTACCTCGTTCCGGGGTATGCAAc                                                    <  2:162590/151‑1 (MQ=255)                                                                                             gtgCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTAAGGCGGCGGGGGGGAAAAACGGGTTGACGGCTATCACCGTTGGGGGGCTGTTCCTCCTGGTTTTTTTTCTCTCTCCGCTCTCTTTCCTCCGTTCGGGGGATGCAACgg                                                  >  1:202581/1‑150 (MQ=255)                                                                                                cGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGGACGGCGGCGGGGGGGAAAACCGGGTTTTCGGCCATCACCGGTGGCGTGCTGTTCCTCCTGCTTCTGTTCCTCTCTCCCCCCTCTTTCCTCGTTCCGGGGTATGCAACGGCTc                                               >  1:314398/1‑150 (MQ=255)                                                                                                           gCGGTATACATCGAGTCTGCGGCGGGTACGGCGGCGGGGGGGAAAACCGGTTTGACGGCTATCACCCTTGGCGGGGTGTTTCTCCCGATTCTTTTTCTCTCTCCCCCCTCTTTACTCGTTTCGGGGGATGCAACGGCCCCCGCGCCGgtgt                                   >  2:138909/1‑151 (MQ=255)                                                                                                                              cggcggGTACGGCGGCGGGCGGTAAAACCGGTTTGACGGCTATCACCGTTGGCGTGCTGTTCCTCCTGATTCTGTTCCTCTCTCCGCTCTCTTACCTCGTTCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCtgctgatgc                 <  1:199978/150‑1 (MQ=255)                                                                                                                                    gTACGGCGGCGGGCGGTAAAACCGGTTTGACGGCTATCACCGTTGGCGTGCTGTTCCTCCTGATTCTGTTCCTCTCTCCGCTCTCTTACCTCGTTCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCaa          >  1:42287/1‑151 (MQ=255)                                                                                                                                             cgggcggTAAAACCGGTTTGACGGCTATCACCGTTGGCGTGCTGTTCCTCCTGATTCTGTTCCTCTCTCCGCTCTCTTACCTCGTTCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGa  <  2:146032/150‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                  GGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTACGGCGGCGGGCGGTAAAACCGGTTTGACGGCTATCACCGTTGGCGTGCTGTTCCTCCTGATTCTGTTCCTCTCTCCGCTCTCTTACCTCGTTCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGA  >  NC_000913/4279324‑4279613

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑