Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,279,4680CG55.6% 14.9 / 26.3 18G330G (GGC→GGGghxPguanine/hypoxanthine permease, high affinity; guanine/hypoxanthine:H+ symporter
Reads supporting (aligned to +/- strand):  ref base C (2/6);  new base G (10/0);  total (12/6)
Fisher's exact test for biased strand distribution p-value = 1.51e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.11e-03

GGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTACGGCGGCGGGCGGTAAAACCGGTTTGACGGCTATCACCGTTGGCGTGCTGTTCCTCCTGATTCTGTTCCTCTCTCCGCTCTCTTACCTCGTTCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGA  >  NC_000913/4279324‑4279613
                                                                                                                                                |                                                                                                                                                 
ggCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTACGGCGGGGGGGGGTaaa                                                                                                                                             >  1:92899/1‑151 (MQ=255)
         gctgGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGGACGGCGGGGGGGGGGAAAAACGGgttt                                                                                                                                    >  1:168796/1‑150 (MQ=255)
            ggATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGGACGGCGGGGGGGGGGGAAACCCGGTTGGCg                                                                                                                                 >  2:206112/1‑151 (MQ=255)
              aTAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTACGGCGGCGGGCGGTAAAACCGGTTTGACgg                                                                                                                                <  2:168795/150‑1 (MQ=255)
                               atcGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTACGGCGGCGGGCGGTAAAACCGGTTTGACGGCTATCACCGTTGGCGTGc                                                                                                              <  2:147147/151‑1 (MQ=255)
                                        gggAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTACGGCGGCGGGCGGGAAAACCGGGTTGACGGCTCTCACCGTTTGGGTGCTGTtcctc                                                                                                      >  1:232943/1‑150 (MQ=255)
                                         ggAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTACGGCGGCGGGGGGTAAAACCGGGTTGACGGCTATCACCGCTGGCGGGCCGTtcctcc                                                                                                     >  2:123308/1‑150 (MQ=255)
                                                            cTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGGACGGCGGCGGGGGGGAAAAACGGGTTGACGGCTATCACCGCTGGGGGGCTGTTTCCCCTGATTCTGTTTCTCTCTcc                                                                                  >  1:137697/1‑150 (MQ=255)
                                                                              ctctGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTACGGCGGGGGGGGGGAAAAACGGGTTGACGGCTATCACCGTTGGCGGGCTGTTCCTCCCTATTCTGTTCCCCTCTCCGCCCTCTTTCCTCGtttc                                                                >  1:32248/1‑148 (MQ=255)
                                                                                 tGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTACGGCGGCGGGCGGTAAAACCGGTTTGACGGCTATCACCGTTGGCGTGCTGTTCCTCCTGATTCTGTTCCTCTCTCCGCTCTCTTACCTCGTTCCgggg                                                            <  1:228464/151‑1 (MQ=255)
                                                                                  ggCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGGACGGCGGGGGGGGGGAAAACCGGGTTTACGGGTATTACCGGTTGGGGGCTGGTTCTCCCGATTTTGTTTCTCTCTCCCCTCTCTTTCCTCGTTTCggggg                                                           >  1:56207/1‑150 (MQ=255)
                                                                                   gCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTACGGCGGCGGGCGGTAAAACCGGTTTGACGGCTATCACCGTTGGCGTGCTGTTCCTCCTGATTCTGTTCCTCTCTCCGCTCTCTTACCTCGTTCCGGGGt                                                           <  2:231315/150‑1 (MQ=255)
                                                                                         tgggtgCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTACGGCGGCGGGCGGTAAAACCGGTTTGACGGCTATCACCGTTGGCGTGCTGTTCCTCCTGATTCTGTTCCTCTCTCCGCTCTCTTACCTCGTTCCGGGGTATGCAAc                                                    <  2:162590/151‑1 (MQ=255)
                                                                                            gtgCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTAAGGCGGCGGGGGGGAAAAACGGGTTGACGGCTATCACCGTTGGGGGGCTGTTCCTCCTGGTTTTTTTTCTCTCTCCGCTCTCTTTCCTCCGTTCGGGGGATGCAACgg                                                  >  1:202581/1‑150 (MQ=255)
                                                                                               cGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGGACGGCGGCGGGGGGGAAAACCGGGTTTTCGGCCATCACCGGTGGCGTGCTGTTCCTCCTGCTTCTGTTCCTCTCTCCCCCCTCTTTCCTCGTTCCGGGGTATGCAACGGCTc                                               >  1:314398/1‑150 (MQ=255)
                                                                                                          gCGGTATACATCGAGTCTGCGGCGGGTACGGCGGCGGGGGGGAAAACCGGTTTGACGGCTATCACCCTTGGCGGGGTGTTTCTCCCGATTCTTTTTCTCTCTCCCCCCTCTTTACTCGTTTCGGGGGATGCAACGGCCCCCGCGCCGgtgt                                   >  2:138909/1‑151 (MQ=255)
                                                                                                                             cggcggGTACGGCGGCGGGCGGTAAAACCGGTTTGACGGCTATCACCGTTGGCGTGCTGTTCCTCCTGATTCTGTTCCTCTCTCCGCTCTCTTACCTCGTTCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCtgctgatgc                 <  1:199978/150‑1 (MQ=255)
                                                                                                                                   gTACGGCGGCGGGCGGTAAAACCGGTTTGACGGCTATCACCGTTGGCGTGCTGTTCCTCCTGATTCTGTTCCTCTCTCCGCTCTCTTACCTCGTTCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCaa          >  1:42287/1‑151 (MQ=255)
                                                                                                                                            cgggcggTAAAACCGGTTTGACGGCTATCACCGTTGGCGTGCTGTTCCTCCTGATTCTGTTCCTCTCTCCGCTCTCTTACCTCGTTCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGa  <  2:146032/150‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
GGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTACGGCGGCGGGCGGTAAAACCGGTTTGACGGCTATCACCGTTGGCGTGCTGTTCCTCCTGATTCTGTTCCTCTCTCCGCTCTCTTACCTCGTTCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGA  >  NC_000913/4279324‑4279613

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: