Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	501,564	0	A	C	58.8%	8.6 / 17.1	17	*559E (TAA→GAA)	ybaL	inner membrane putative NAD(P)‑binding transporter
Reads supporting (aligned to +/- strand):  ref base A (2/5);  new base C (0/10);  total (2/15)
Fisher's exact test for biased strand distribution p-value = 1.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.27e-03

CTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATG  >  NC_000913/501417‑501693                                                                                                                                                    |                                                                                                                                  cTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTAccc                                                                                                                                <  2:31865/151‑1 (MQ=255) cTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTAccc                                                                                                                                <  1:80840/151‑1 (MQ=255)        cGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTcacc                                                                                                                         <  1:196558/151‑1 (MQ=255)        cGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTcacc                                                                                                                         <  2:111740/151‑1 (MQ=255)        cGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTcac                                                                                                                          >  2:12020/1‑150 (MQ=255)           tAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCaccacc                                                                                                                      <  2:61715/151‑1 (MQ=255)                                    gATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCcagca                                                                                             >  2:183013/1‑151 (MQ=255)                                             ttCCCGCCGCATCTGGCTTTTGGCTCTCGATGCCTGATGCGACGCGGGCGCGTCTTATCATCCCTACATTTTTTTCATATTTTCCATCCGGCACCCCCCGTTTCCCCCGCCCCCCCCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCa                                                                                    <  2:70139/151‑1 (MQ=255)                                                      cATCTGGCTTTTGGCTCTCGATGCCTGATGCGCCGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACACCCGGCACCCACCGTTTCCCCCGTCCCCACCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACggg                                                                           <  2:284794/151‑1 (MQ=39)                                                                 tGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCTTTTCCCCCGTCACCACCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGc                                                                <  1:29886/151‑1 (MQ=255)                                                                             ccTGATGCGCCGCGGGCGCGTTTTATCATGCCTACATTTTTTTCATATTTTACATCCGGCACCCCCCGTTTCCCCCGTCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACt                                                    <  1:17542/151‑1 (MQ=255)                                                                                           ggCGCGTCTTATCATGCCTACATTTTTTTCATATTTTACACCCGGCAACCCCCTTTTCCCCCGTCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGcac                                       <  2:53653/150‑1 (MQ=255)                                                                                                     aTCATGCCTACATATTTTTCATTTTTTACATCCGGCACCCCCCGTTTCCCCCGTCACCCCCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTg                            <  1:110584/151‑1 (MQ=255)                                                                                                         tGCCTACATATTTTTCATATTTTACATCCGGCACCCACCGTTTCCCCCGTCCCCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGatat                        <  1:100757/151‑1 (MQ=255)                                                                                                           ccTACATATTTTTCATATTTTACATCCGGCACCCACCGTTTCCCCCGTCACCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATAc                      <  1:253656/151‑1 (MQ=255)                                                                                                           ccTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTCCCCCGTCACCACCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATAc                      <  1:58847/151‑1 (MQ=255)                                                                                                                               tACACCCGGCAACCCCCGTTTCCCCCGTCACCCCCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATg  <  1:159533/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                  CTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATG  >  NC_000913/501417‑501693

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑