Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	849,300	T→G	L266L (CTA→CTC)	rhtA ←	threonine and homoserine efflux system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	849,300	0	T	G	100.0%	66.2 / NA	19	L266L (CTA→CTC)	rhtA	threonine and homoserine efflux system
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (12/7);  total (12/7)

AGGAAAAGTAGCGAGAAATTCTGCATGGTTATGCATAACCATGCAGAAATGTAAATTTAATTAATGTCTAATTCTTTTATTTTGCTCTCTTTGCGTACTGTCAGCGTAGACCCCATTGAAGCGGCGATGATAGCGCCGAGCGCCAGTAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGC  >  NC_000913/849154‑849435                                                                                                                                                   |                                                                                                                                        agGAAAAGTAGCGAGAAATTCTGCATGGTTATGCATAACCATGCAGAAATGTAAATTTAATTAATGTCTAATTCTTTTATTTTGCTCTCTTTGCGTACTGTCAGCGTAGACCCCATTGAAGCGGCGATGATAGCGCCGAGCGCCAGGAGc                                                                                                                                      <  2:33861/150‑1 (MQ=255)         tAGCGAGAAATTCTGCATGGTTATGCATAACCATGCAGAAATGTAAATTTAATTAATGTCTAATTCTTTTATTTTGCTCTCTTTGCGTACTGTCAGCGTAGACCCCATTGAAGCGGCGATGATAGCGCCGAGCGCCAGGAGCTGTATggg                                                                                                                              <  1:236763/150‑1 (MQ=255)         tAGCGAGAAATTCTGCATGGTTATGCATAACCATGCAGAAATGTAAATTTAATTAATGTCTAATTCTTTTATTTTGCTCTCTTTGCGTACTGTCAGCGTAGACCCCATTGAAGCGGCGATGATAGCGCCGAGCGCCAGGAGCTGTATggg                                                                                                                              <  1:316631/150‑1 (MQ=255)            cGAGAAATTCTGCATGGTTATGCATAACCATGCAGAAATGTAAATTTAATTAATGTCTAATTCTTTTATTTTGCTCTCTTTGCGTACTGTCAGCGTAGACCCCATTGAAGCGGCGATGATAGCGCCGAGCGCCAGGAGCTGTATGGgtg                                                                                                                            <  1:99538/149‑1 (MQ=255)                            gTTATGCATAACCATGCAGAAATGTAAATTTAATTAATGTCTAATTCTTTTATTTTGCTCTCTTTGCGTACTGTCAGCGTAGACCCCATTGAAGCGGCGATGATAGCGCCGAGCGCCAGGAGCTGTATGGGTGTCAGTGTTTCTCCGAGGa                                                                                                          >  1:90925/1‑151 (MQ=255)                            gTTATGCATAACCATGCAGAAATGTAAATTTAATTAATGTCTAATTCTTTTATTTTGCTCTCTTTGCGTACTGTCAGCGTAGACCCCATTGAAGCGGCGATGATAGCGCCGAGCGCCAGGAGCTGTATGGGTGTCAGTGTTTCTCCGAGGa                                                                                                          >  1:76162/1‑151 (MQ=255)                               aTGCATAACCATGCAGAAATGTAAATTTAATTAATGTCTAATTCTTTTATTTTGCTCTCTTTGCGTACTGTCAGCGTAGACCCCATTGAAGCGGCGATGATAGCGCCGAGCGCCAGGAGCTGTATGGGTGTCAGTGTTTCTCCGAGGaaaa                                                                                                       >  1:52694/1‑151 (MQ=255)                               aTGCATAACCATGCAGAAATGTAAATTTAATTAATGTCTAATTCTTTTATTTTGCTCTCTTTGCGTACTGTCAGCGTAGACCCCATTGAAGCGGCGATGATAGCGCCGAGCGCCAGGAGCTGTATGGGTGTCAGTGTTTCTCCGAGGaaaa                                                                                                       >  2:119142/1‑151 (MQ=255)                               aTGCATAACCATGCAGAAATGTAAATTTAATTAATGTCTAATTCTTTTATTTTGCTCTCTTTGCGTACTGTCAGCGTAGACCCCATTGAAGCGGCGATGATAGCGCCGAGCGCCAGGAGCTGTATGGGTGTCAGTGTTTCTCCGAGGaaaa                                                                                                       >  2:20222/1‑151 (MQ=255)                                      aCCATGCAGAAATGTAAATTTAATTAATGTCTAATTCTTTTATTTTGCTCTCTTTGCGTACTGTCAGCGTAGACCCCATTGAAGCGGCGATGATAGCGCCGAGCGCCAGGAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATcc                                                                                                 >  1:71698/1‑150 (MQ=255)                                      aCCATGCAGAAATGTAAATTTAATTAATGTCTAATTCTTTTATTTTGCTCTCTTTGCGTACTGTCAGCGTAGACCCCATTGAAGCGGCGATGATAGCGCCGAGCGCCAGGAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATcc                                                                                                 >  2:101234/1‑150 (MQ=255)                                      aCCATGCAGAAATGTAAATTTAATTAATGTCTAATTCTTTTATTTTGCTCTCTTTGCGTACTGTCAGCGTAGACCCCATTGAAGCGGCGATGATAGCGCCGAGCGCCAGGAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATcc                                                                                                 >  2:307602/1‑150 (MQ=255)                                                                   tCTAATTCTTTTATTTTGCTCTCTTTGCGTACTGTCAGCGTAGACCCCATTGAAGCGGCGATGATAGCGCCGAGCGCCAGGAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGc                                                                   <  1:46822/151‑1 (MQ=255)                                                                                         ctTTGCGTACTGTCAGCGTAGACCCCATTGAAGCGGCGATGATAGCGCCGAGCGCCAGGAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCg                                             >  1:303716/1‑151 (MQ=255)                                                                                                  cTGTCAGCGTAGACCCCATTGAAGCGGCGATGATAGCGCCGAGCGCCAGGAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCaa                                    >  1:70527/1‑151 (MQ=255)                                                                                                        gCGTAGACCCCATTGAAGCGGCGATGATAGCGCCGAGCGCCAGGAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGt                              >  1:56848/1‑151 (MQ=255)                                                                                                        gCGTAGACCCCATTGAAGCGGCGATGATAGCGCCGAGCGCCAGGAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGt                              >  2:314057/1‑151 (MQ=255)                                                                                                                          cggcgATGATAGCGCCGAGCGCCAGGAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTcc              <  1:12188/149‑1 (MQ=255)                                                                                                                                      cgcCGAGCGCCAGGAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGc  <  2:211823/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                        AGGAAAAGTAGCGAGAAATTCTGCATGGTTATGCATAACCATGCAGAAATGTAAATTTAATTAATGTCTAATTCTTTTATTTTGCTCTCTTTGCGTACTGTCAGCGTAGACCCCATTGAAGCGGCGATGATAGCGCCGAGCGCCAGTAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGC  >  NC_000913/849154‑849435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑