Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 850,651 T→G Y68D (TAC→GAC)  ompX → outer membrane protein X

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913850,6510TG100.0% 46.9 / NA 14Y68D (TAC→GAC) ompXouter membrane protein X
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (7/7);  total (7/7)

GGTACTTCCGTAGCTGCGACTTCTACTGTAACTGGCGGTTACGCACAGAGCGACGCTCAGGGCCAAATGAACAAAATGGGCGGTTTCAACCTGAAATACCGCTATGAAGAAGACAACAGCCCGCTGGGTGTGATCGGTTCTTTCACTTACACCGAGAAAAGCCGTACTGCAAGCTCTGGTGACTACAACAAAAACCAGTACTACGGCATCACTGCTGGTCCGGCTTACCGCATTAACGACTGGGCAAGCATCTACGGTGTAGTGGGTGTGGGTTATGGTAAATTCCAGAC  >  NC_000913/850504‑850793
                                                                                                                                                   |                                                                                                                                              
ggTACTTCCGTAGCTGCGACTTCTACTGTAACTGGCGGTTACGCACAGAGCGACGCTCAGGGCCAAATGAACAAAATGGGCGGTTTCAACCTGAAATACCGCTATGAAGAAGACAACAGCCCGCTGGGTGTGATCGGTTCTTTCACTGaca                                                                                                                                             >  1:97984/1‑151 (MQ=255)
                 gACTTCTACTGTAACTGGCGGTTACGCACAGAGCGACGCTCAGGGCCAAATGAACAAAATGGGTGGTTTCAACCTGAAATACCGCTATGAAGAAGACAACAGCCCGCTGGGTGTGATCGGTTCTTTCACTGACACCGAGAAAAGCCGTa                                                                                                                              >  1:113859/1‑149 (MQ=255)
                              aCTGGCGGTTACGCACAGAGCGACGCTCAGGGCCAAATGAACAAAATGGGCGGTTTCAACCTGAAATACCGCTATGAAGAAGACAACAGCCCGCTGGGTGTGATCGGTTCTTTCACTGACACCGAGAAAAGCCGTACTGCAAGCTCTGGTg                                                                                                               <  2:281750/151‑1 (MQ=255)
                                                                                         ccTGAAATACCGCTATGAAGAAGACAACAGCCCGCTGGGTGTGATCGGTTCTTTCACTGACACCGAGAAAAGCCGTACTGCAAGCTCTGGTGACTACAACAAAAACCAGTACTACGGCATCACTGCTGGTCCGGCTTACCGCATTAACGAc                                                    <  2:201928/151‑1 (MQ=255)
                                                                                                  ccGCTATGAAGAAGACAACAGCCCGCTGGGTGTGATCGGTTCTTTCACTGACACCGAGAAAAGCCGTACTGCAAGCTCTGGTGACTACAACAAAAACCAGTACTACGGCATCACTGCTGGTCCGGCTTACCGCATTAACGACTGGGCAAg                                            >  2:276618/1‑150 (MQ=255)
                                                                                                  ccGCTATGAAGAAGACAACAGCCCGCTGGGTGTGATCGGTTCTTTCACTGACACCGAGAAAAGCCGTACTGCAAGCTCTGGTGACTACAACAAAAACCAGTACTACGGCATCACTGCTGGTCCGGCTTACCGCATTAACGACTGGGCAAg                                            >  2:276619/1‑150 (MQ=255)
                                                                                                         gaagaaGACAACAGCCCGCTGGGTGTGATCGGTTCTTTCACTGACACCGAGAAAAGCCGTACTGCAAGCTCTGGTGACTACAACAAAAACCAGTACTACGGCATCACTGCTGGTCCGGCTTACCGCATTAACGACTGGGCAAGCATCTACg                                    >  2:184855/1‑151 (MQ=255)
                                                                                                                       cccGCTGGGTGTGATCGGTTCTTTCACTGACACCGAGAAAAGCCGTACTGCAAGCTCTGGTGACTACAACAAAAACCAGTACTACGGCATCACTGCTGGTCCGGCTTACCGCATTAACGACTGGGCAAGCATCTACGGTGTAgtggg                          <  2:110411/147‑1 (MQ=255)
                                                                                                                       cccGCTGGGTGTGATCGGTTCTTTCACTGACACCGAGAAAAGCCGTACTGCAAGCTCTGGTGACTACAACAAAAACCAGTACTACGGCATCACTGCTGGTCCGGCTTACCGCATTAACGACTGGGCAAGCATCTACGGTGTAgtggg                          <  2:38267/147‑1 (MQ=255)
                                                                                                                       cccGCTGGGTGTGATCGGTGCTTTCACTGACACCGAGAAAAGCCGTACTGCAAGCTCTGGTGACTACAACAAAAACCAGTACTACGGCATCACTGCTGGTCCGGCTTACCGCATTAACGACTGGGCAAGCATCTACGGTGTAgtgggtg                        <  2:82804/149‑1 (MQ=255)
                                                                                                                                tgtgATCGGTTCTTTCACTGACACCGAGAAAAGCCGTACTGCAAGCTCTGGTGACTACAACAAAAACCAGTACTACGGCATCACTGCTGGTCCGGCTTACCGCATTAACGACTGGGCAAGCATCTACGGTGTAGTGGGTGTGGGTTATgg              <  2:55627/150‑1 (MQ=255)
                                                                                                                                           cTTTCACTGGCACCGAGAAAAGCCGTACTGCAAGCTCTGGTGACTACAACAAAAACCAGTACTACGGCATCACTGCTGGTCCGGCTTACCGCATTAACGACTGGGCAAGCATCTACGGTGTAGTGGGTGTGGGTTATGGTAAATTCCAGa   >  1:167012/1‑150 (MQ=255)
                                                                                                                                           cTTTCACTGACACCGAGAAAAGCCGTACTGCAAGCTCTGGTGACTACAACAAAAACCAGTACTACGGCATCACTGCTGGTCCGGCTTACCGCATTAACGACTGGGCAAGCATCTACGGTGTAGTGGGTGTGGGTTATGGTAAATTCCAGa   >  2:158478/1‑150 (MQ=255)
                                                                                                                                           cTTTCACTGACACCGAGAAAAGCCGTACTGCAAGCTCTGGTGACTACAACAAAAACCAGTACTACGGCATCACTGCTGGTCCGGCTTACCGCATTAACGACTGGGCAAGCATCTACGGTGTAGTGGGTGTGGGTTATGGTAAATTCCAGAc  <  1:118059/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                              
GGTACTTCCGTAGCTGCGACTTCTACTGTAACTGGCGGTTACGCACAGAGCGACGCTCAGGGCCAAATGAACAAAATGGGCGGTTTCAACCTGAAATACCGCTATGAAGAAGACAACAGCCCGCTGGGTGTGATCGGTTCTTTCACTTACACCGAGAAAAGCCGTACTGCAAGCTCTGGTGACTACAACAAAAACCAGTACTACGGCATCACTGCTGGTCCGGCTTACCGCATTAACGACTGGGCAAGCATCTACGGTGTAGTGGGTGTGGGTTATGGTAAATTCCAGAC  >  NC_000913/850504‑850793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: