Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	926,224	A→C	intergenic (‑253/+1)	serW ← / ← infA	tRNA‑Ser/translation initiation factor IF‑1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	926,224	0	A	C	100.0%	96.6 / NA	27	intergenic (‑253/+1)	serW/infA	tRNA‑Ser/translation initiation factor IF‑1
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (10/17);  total (10/17)

CTGTAAACAAGTTGTGCAGTATATCTACATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAATCAGCGACTACGGAAGACAATGCGGCCTTTGCTCAGGTCGTACGGGGTCAGTTCAACAGTCACTTTGTCGCCCGTCAGGATGCGGATGTAGTTTTTGCGCATTTTACCGGAGATGTGTGCAGTAACCACGTGACCGTTTTCTAACTC  >  NC_000913/926078‑926371                                                                                                                                                   |                                                                                                                                                    cTGTAAACAAGTTGTGCAGTATATCTACATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACACTCAg                                                                                                                                                 >  1:168693/1‑151 (MQ=255)   gTAAACAAGTTGTGCAGTATATCTACATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACACTCAGCg                                                                                                                                               >  1:143052/1‑151 (MQ=255)    tAAACAAGTTGTGCAGTATATCTACATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACACTCAGCg                                                                                                                                               <  1:184995/150‑1 (MQ=255)              gtgCAGTATATCTACATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACACTCAGCGACTACGGAAGa                                                                                                                                    >  1:93634/1‑151 (MQ=255)               tgCAGTATATCTACATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACACTCAGCGACTACGGAAGAc                                                                                                                                   <  2:296783/151‑1 (MQ=255)               tgCAGTATATCTACATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACACTCAGCGACTACGGAAGAc                                                                                                                                   <  1:160208/151‑1 (MQ=255)               tgCAGTATATCTACATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACACTCAGCGACTACGGAAGAc                                                                                                                                   <  1:309504/151‑1 (MQ=255)                gCAGTATATCTACATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACACTCAGCGACTACGGAAGACa                                                                                                                                  >  2:98677/1‑151 (MQ=255)                   gTATATCTACTTCGAGACAAGTTACGGACTTATACTTCCAACGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTTCTCGTTCTTTCTCTTCGTCCTTCCGGCGATAAAACACTCATCGACTACAGAAGACAATg                                                                                                                               >  2:149679/1‑151 (MQ=255)                   gTATATCTACATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACACTCAGCGACTACGGAAGACAATg                                                                                                                               >  1:291312/1‑151 (MQ=255)                     atatCTACATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACACTCAGCGACTACGGAAGACAATGCg                                                                                                                             >  1:305067/1‑151 (MQ=255)                      tatCTACATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACACTCAGCGACTACGGAAGACAATGCgg                                                                                                                            >  2:273857/1‑151 (MQ=255)                              tCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACACTCAGCGACTACGGAAGACAATGCGGCCTTTGCt                                                                                                                    <  2:277540/151‑1 (MQ=255)                                         tACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACACTCAGCGACTACGGAAGACAATGCGGCCTTTGCTCAGGTCGTACg                                                                                                         <  1:128099/151‑1 (MQ=255)                                                  ataCTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACACTCAGCGACTACGGAAGACAATGCGGCCTTTGCTCAGGTCGTACGGGGTCAGtt                                                                                                <  1:53169/151‑1 (MQ=255)                                                      ttCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACACTCAGCGACTACGGAAGACAATGCGGCCTTTGCTCAGGTCGTACGGGGTCAGTTCAAc                                                                                            <  1:127629/151‑1 (MQ=255)                                                        ccAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACACTCAGCGACTACGGAAGACAATGCGGCCTTTGCTCAGGTCGTACGGGGTCAGTTCAACAg                                                                                          <  2:108731/151‑1 (MQ=255)                                                        ccAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACACTCAGCGACTACGGAAGACAATGCGGCCTTTGCTCAGGTCGTACGGGGTCAGTTCAACAg                                                                                          <  2:37636/151‑1 (MQ=255)                                                                cTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACACTCAGCGACTACGGAAGACAATGCGGCCTTTGCTCAGGTCGTACGGGGTCAGTTCAACAGTCACTTTg                                                                                  >  2:247268/1‑151 (MQ=255)                                                                    atacataTCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACACTCAGCGACTACGGAAGACAATGCGGCCTTTGCTCAGGTCGTACGGGGTCAGTTCAACAGTCACTTTGTCGc                                                                              <  2:91497/151‑1 (MQ=255)                                                                     tacataTCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACACTCAGCGACTACGGAAGACAATGCGGCCTTTGCTCAGGTCGTACGGGGTCAGTTCAACAGTCACTTTGTCGcc                                                                             <  1:117830/151‑1 (MQ=255)                                                                            cacaAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACACTCAGCGACTACGGAAGACAATGCGGCCTTTGCTCAGGTCGTACGGGGTCAGTTCAACAGTCACTTTGTCGCCCGTCAgg                                                                      <  2:63396/151‑1 (MQ=255)                                                                                                                     ctttctCTTCGCCCATCAGGCGGTAAAACACTCAGCGACTACGGAAGACAATGCGGCCTTTGCTCAGGTCGTACGGGGTCAGTTCAACAGTCACTTTGTCGCCCGTCAGGATGCGGATGTAGTTTTTGCGCATTTTACCGGAGATGTGTGc                             <  1:232687/151‑1 (MQ=255)                                                                                                                       ttctCTTCGCCCATCAGGCGGTAAAACACTCAGCGACTACGGAAGACAATGCGGCCTTTGCTCAGGTCGTACGGGGTCAGTTCAACAGTCACTTTGTCGCCCGTCAGGATGCGGATGTAGTTTTTGCGCATTTTACCGGAGATGTGTGCAg                           <  2:168692/151‑1 (MQ=255)                                                                                                                       ttctCTTCGCCCATCAGGCGGTAAAACACTCAGCGACTACGGAAGACAATGCGGCCTTTGCTCAGGTCGTACGGGGTCAGTTCAACAGTCACTTTGTCGCCCGTCAGGATGCGGATGTAGTTTTTGCGCATTTTACCGGAGATGTGTGCAg                           <  2:93633/151‑1 (MQ=255)                                                                                                                                    tCAGGCGGTAAAACACTCAGCGACTACGGAAGACAATGCGGCCTTTGCTCAGGTCGTACGGGGTCAGTTCAACAGTCACTTTGTCGCCCGTCAGGATGCGGATGTAGTTTTTGCGCATTTTACCGGAGATGTGTGCAGTAACCACGTGAcc              <  2:221311/151‑1 (MQ=255)                                                                                                                                           gTAAAACACTCAGCGACTACGGAAGACAATGCGGCCTTTGCTCAGGTCGTACGGGGTCAGTTCAACAGTCACTTTGTCGCCCGTCAGGATGCGGATGTAGTTTTTGCGCATTTTACCGGAGATGTGTGCAGTAACCACGTGACCGtttt         >  1:98675/1‑149 (MQ=255)                                                                                                                                                acacTCGGCGACTACGGAAGACAATGCGGCCTTTGCTCAGGTCGTACGGGGTCAGTTCAACAGTCACTTTGTCGCCCGTCAGGATGCGGATGTAGTTTTTGCGCATTTTACCGGAGATGTGTGCAGTAACCACGTGACCGTTTTCTAActc  <  1:253460/151‑1 (MQ=255)                                                                                                                                                acacTCAGCGACTACGGAAGACAATGCGGCCTTTGCTCAGGTCGTACGGGGTCAGTTCAACAGTCACTTTGTCGCCCGTCAGGATGCGGATGTAGTTTTTGCGCATTTTACCGGAGATGTGTGCAGTAACCACGTGACCGTTTTCTAActc  <  1:306463/151‑1 (MQ=255)                                                                                                                                                acacTCAGCGACTACGGAAGACAATGCGGCCTTTGCTCAGGTCGTACGGGGTCAGTTCAACAGTCACTTTGTCGCCCGTCAGGATGCGGATGTAGTTTTTGCGCATTTTACCGGAGATGTGTGCAGTAACCACGTGACCGTTTTCTAActc  <  1:25971/151‑1 (MQ=255)                                                                                                                                                acacTCAGCGACTACGGAAGACAATGCGGCCTTTGCTCAGGTCGTACGGGGTCAGTTCAACAGTCACTTTGTCGCCCGTCAGGATGCGGATGTAGTTTTTGCGCATTTTACCGGAGATGTGTGCAGTAACCACGTGACCGTTTTCTAActc  <  1:122137/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                    CTGTAAACAAGTTGTGCAGTATATCTACATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAATCAGCGACTACGGAAGACAATGCGGCCTTTGCTCAGGTCGTACGGGGTCAGTTCAACAGTCACTTTGTCGCCCGTCAGGATGCGGATGTAGTTTTTGCGCATTTTACCGGAGATGTGTGCAGTAACCACGTGACCGTTTTCTAACTC  >  NC_000913/926078‑926371

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑