Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	23,544	C→T	A385V (GCG→GTG)	ileS →	isoleucyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	23,544	0	C	T	100.0%	106.8 / NA	30	A385V (GCG→GTG)	ileS	isoleucyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/14);  total (16/14)

CTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCA  >  NC_000913/23416‑23688                                                                                                                                 |                                                                                                                                                 cTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGTGCTGCTGCAGGAAAAAGgcgcg                                                                                                                            >  2:109346/1‑151 (MQ=255) cTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGTGCTGCTGCAGGAAAAAGgcgcg                                                                                                                            >  1:310950/1‑151 (MQ=255) cTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGTGCTGCTGCAGGAAAAAGgcgcg                                                                                                                            >  1:250593/1‑151 (MQ=255)         tCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGTGCTGCTGCAGGAAAAAGGCGCGCTGCTGCa                                                                                                                    >  1:125547/1‑151 (MQ=255)              cAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGTGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTg                                                                                                               >  2:101294/1‑151 (MQ=255)                gAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGTGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTgag                                                                                                             >  1:193568/1‑151 (MQ=255)                  aaTACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGTGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGaa                                                                                                           <  2:46595/151‑1 (MQ=255)                             gAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGTGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGcac                                                                                                 <  1:15204/150‑1 (MQ=255)                                                                       ggCACTTATCCGACGCTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGTGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTc                                                       <  2:251712/150‑1 (MQ=255)                                                                       ggCACTTATCCGACGCTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGTGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTc                                                       <  1:251707/150‑1 (MQ=255)                                                                        gCACTTATCCGACGCTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGTGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCcg                                                     >  1:101576/1‑151 (MQ=255)                                                                           cTTATCCGACGCTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGTGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCcgcg                                                   >  1:313010/1‑150 (MQ=255)                                                                              aTCCGACGCTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGTGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGa                                                >  2:30673/1‑150 (MQ=255)                                                                                   aCGCTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGTGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGAcgccg                                           >  2:98002/1‑150 (MQ=255)                                                                                   aCGCTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGTGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGAcgccg                                           >  2:199468/1‑150 (MQ=255)                                                                                   aCGCTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGTGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGAcgccg                                           >  1:304503/1‑150 (MQ=255)                                                                                      cTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGTGCTGCTGCAGGAAAAAGGCGGGCTGCTGCACGTTGAGAAAATGCAGCGCAGCTCTCCGTGCTGGTTGTGTCACAAAGCGCCGATCATCTTCCGCGCGACGCCGCAg                                        <  2:40863/150‑1 (MQ=255)                                                                                      cTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGTGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAg                                        <  2:40864/150‑1 (MQ=255)                                                                                         gATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGTGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTgg                                     <  2:206466/150‑1 (MQ=255)                                                                                                      tCTTCAAAGCGAACGACATCGTCGTTGTGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGa                       >  2:317888/1‑151 (MQ=255)                                                                                                       cTTCAAAGCGAACGACATCGTCGTTGTGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGAt                      >  1:229106/1‑151 (MQ=255)                                                                                                        ttCAAAGCGAACGACATCGTCGTTGTGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATc                     <  1:192973/151‑1 (MQ=255)                                                                                                            aaGCGAACGACATCGTCGTTGTGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGaa                 <  1:38423/151‑1 (MQ=255)                                                                                                              gCGAACGACATCGTCGTTGTGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAg               >  2:274960/1‑151 (MQ=255)                                                                                                               cGAACGACATCGTCGTTGTGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGaaa                <  2:290271/149‑1 (MQ=255)                                                                                                               cGAACGACATCGTCGTTGTGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAgg              <  2:201968/151‑1 (MQ=255)                                                                                                               cGAACGACATCGTCGTTGTGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAgg              <  2:310948/151‑1 (MQ=255)                                                                                                                 aaCGACATCGTCGTTGTGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTc            <  1:109347/151‑1 (MQ=255)                                                                                                                     aCATCGTCGTTGTGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCtgcg        <  1:227816/151‑1 (MQ=255)                                                                                                                           tcgtTGTGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCa  >  2:42548/1‑151 (MQ=255)                                                                                                                                 |                                                                                                                                                 CTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCA  >  NC_000913/23416‑23688

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑