Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,283,279 T→G F1139C (TTC→TGC)  narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,283,2790TG100.0% 49.1 / NA 15F1139C (TTC→TGC) narGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (4/11);  total (4/11)

CTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGCGTGGTGGTATCCATAACT  >  NC_000913/1283131‑1283422
                                                                                                                                                    |                                                                                                                                               
cTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTGCa                                                                                                                                               <  2:192447/151‑1 (MQ=255)
   aCGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTGCAAc                                                                                                                                             <  2:159616/150‑1 (MQ=255)
                   ggggTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGCGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTGCAACAGCAACGGTGCTCTGa                                                                                                                             >  2:57074/1‑150 (MQ=255)
                       tATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTGCAACAGCAACGGTGCTCTGACTGcc                                                                                                                        <  2:304902/151‑1 (MQ=255)
                                 acCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTGCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTg                                                                                                              >  1:114776/1‑151 (MQ=255)
                                                           gaCTTTAGGTCGCGGTGGTCCGGGGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTGCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATg                                                                                    >  2:95743/1‑151 (MQ=255)
                                                                       cggtggtccggtggtcTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGGTAACGTCTGGATTGAAGTCTGCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTAc                                                                        <  2:17946/151‑1 (MQ=255)
                                                                       cggtggtccggtggtcTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTGCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTAc                                                                        <  2:145673/151‑1 (MQ=255)
                                                                       cggtggtccggtggtcTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTGCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTAc                                                                        <  2:218384/151‑1 (MQ=255)
                                                                         gtggtccggtggtcTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTGCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTAcc                                                                       <  2:156695/150‑1 (MQ=255)
                                                                             tccggtggtcTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTGCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCAcgc                                                                   <  1:97687/150‑1 (MQ=255)
                                                                                  tggtcTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTGCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAgg                                                              <  1:201460/150‑1 (MQ=255)
                                                                                    gtcTGGTTGAGTGAAGCCTATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTGCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTGGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGaa                                                            <  1:80743/150‑1 (MQ=255)
                                                                                                       aTGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTGCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTgg                                        >  2:292345/1‑151 (MQ=255)
                                                                                                                                              aaGTCTGCAACAGCAACGGTGCTTTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGCGTGGTGGTATCCATAACt  <  1:112230/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
CTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGCGTGGTGGTATCCATAACT  >  NC_000913/1283131‑1283422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: