Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,486,563	A→C	K1168T (AAA→ACA)	hrpA →	putative ATP‑dependent helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,486,563	0	A	C	100.0%	40.9 / NA	14	K1168T (AAA→ACA)	hrpA	putative ATP‑dependent helicase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (10/4);  total (10/4)

GCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTACGGCAGTGTTCAATATCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACGGCTTCAAACGGCTGGGCGACACGCTGCGATATTTGCAGGCGA  >  NC_000913/1486437‑1486706                                                                                                                               |                                                                                                                                                gCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTACGGCAGTGTTCAATATCAACACACGTCTGAAAGGGCGGGTGGata                                                                                                                          <  1:51707/150‑1 (MQ=255)                                   gcATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTACGGCAGTGTTCAATATCAACACACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAgcg                                                                                       >  1:131034/1‑151 (MQ=255)                                                       aaCTGAACGACACGGTGGTGGATATCGCGAAGCAGGTCGAGCAAATCCTTACGGCAGTGTTCAATATCAACACACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGtat                                                                    <  1:25023/150‑1 (MQ=255)                                                                acacGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTACGGCAGTGTTCAATATCAACACACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGtt                                                           <  1:298004/150‑1 (MQ=255)                                                                     gtggtgGATATTGCGAAGCAGGTCGAGCAAATCCTTACGGCAGTGTTCAATATCAACACACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCa                                                     >  1:282070/1‑151 (MQ=255)                                                                       ggtggATATTGCGAAGCAGGTCGAGCAAATCCTTACGGCAGTGTTCAATATCAACACACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCa                                                     >  1:96419/1‑149 (MQ=255)                                                                                         ggTCGAGCAAATCCTTACGGCAGTGTTCAATATCAACACACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACGGCTTCAAACgg                                 >  2:159767/1‑151 (MQ=255)                                                                                              aGCAAATCCTTACGGCAGTGTTCAATATCAACACACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACGGCTTCAAACGGCTggg                            >  2:20902/1‑151 (MQ=255)                                                                                                     ccTTACGGCAGTGTTCAATATCAACACACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACGGCTTCAAACGGCTGGGCGACACg                     >  1:269404/1‑151 (MQ=255)                                                                                                       ttACGGCAGTGTTCAATATCAACACACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACGGCTTCAAACGGCTGGGCGACACg                     >  1:105132/1‑149 (MQ=255)                                                                                                       ttACGGCAGTGTTCAATATCAACACACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACGGCTTCAAACGGCTGGGCGACACg                     >  1:277815/1‑149 (MQ=255)                                                                                                                tgtTCAATATCAACACACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACGGCTTCAAACGGCTGGGCGACACGCTGCGATAttt          <  1:144958/151‑1 (MQ=255)                                                                                                                 gtTCAATATCAACACACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACGGCTTCAAACGGCTGGGCGACACGCTGCGATATTTg         >  2:256451/1‑151 (MQ=255)                                                                                                                        atCAACACACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACGGCTTCAAACGGCTGGGCGACACGCTGCGATATTTGCAGGCGa  >  1:192378/1‑151 (MQ=255)                                                                                                                               |                                                                                                                                                GCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTACGGCAGTGTTCAATATCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACGGCTTCAAACGGCTGGGCGACACGCTGCGATATTTGCAGGCGA  >  NC_000913/1486437‑1486706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑