Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,541,434	A→C	V386G (GTA→GGA)	narZ ←	nitrate reductase 2 (NRZ), alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,541,434	0	A	C	100.0%	49.0 / NA	15	V386G (GTA→GGA)	narZ	nitrate reductase 2 (NRZ), alpha subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (6/9);  total (6/9)

AAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTCGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACTGCTACGGTTTTCCACTGCGGATTGTTGCTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACC  >  NC_000913/1541290‑1541571                                                                                                                                                 |                                                                                                                                          aaGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTCGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTCCCAAtt                                                                                                                                     >  2:13944/1‑151 (MQ=255)   gTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTCGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTCCCAATTCa                                                                                                                                   >  2:60266/1‑151 (MQ=255)       gggAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTCGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTCCCAATTCAcc                                                                                                                                 <  2:109593/149‑1 (MQ=255)                  gCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTCGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTCCCAATTCACCTGCGGTATTaa                                                                                                                      >  2:254367/1‑149 (MQ=255)                   ccTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTCGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTCCCAATTCACCTGCGGTATTAACt                                                                                                                    <  2:233806/150‑1 (MQ=255)                   ccTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTCGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTCCCAATTCACCTGCGGTATTAACTg                                                                                                                   <  1:178716/151‑1 (MQ=255)                                 aTGTTGACCGAGCAGGGTTAACGACAATTCGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTCCCAATTCACCTGCGGTATTAACTGCTACGGTTTTCCAc                                                                                                     <  1:222670/151‑1 (MQ=255)                                                                    ccGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTCCCAATTCACCTGCGGTATTAACTGCTACGGTTTTCCACTGCGGATTGTTGCTTTCGCCCAGTCCATCCACCAg                                                                  >  1:129164/1‑151 (MQ=255)                                                                             cggcAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTCCCAATTCACCTGCGGTATTAACTGCTACGGTTTTCCACTGCGGATTGTTGCTTTCGCCCAGTCCATCCACCAGGTCAGATg                                                          <  2:77091/150‑1 (MQ=255)                                                                                      attccagattccaTTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTCCCAATTCACCTGCGGTATTAACTGCTGCGGTTTTCCACTGCGGATTGTTGCTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCa                                                 <  1:111330/150‑1 (MQ=255)                                                                                                                            ccAATCGAACCGTTCGGCACTCCCAATTCACCTGCGGTATTAACTGCTACGGTTTTCCACTGCGGATTGTTGCTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCa          >  1:242206/1‑151 (MQ=255)                                                                                                                             cAATCGAACCGTTCGGCACTCCCAATTCACCTGCGGTATTAACTGCTACGGTTTTCCACTGCGGATTGTTGCTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAg         >  1:34096/1‑151 (MQ=255)                                                                                                                                tCGAACCGTTCGGCACTCCCAATTCACCTGCGGTATTAACTGCTACGGTTTTCCACTGCGGATTGTTGCTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCAt      <  1:13944/151‑1 (MQ=255)                                                                                                                                    aCCGTTCGGCACTCCCAATTCACCTGCGGTATTAACTGCTACGGTTTTCCACTGCGGATTGTTGCTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTAcc  <  2:45149/151‑1 (MQ=255)                                                                                                                                    aCCGTTCGGCACTCCCAATTCACCTGCGGTAACAACTGCTACGGTTTTCCACTGCGGATTGTTGCTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCCGATCATGCCCCCGTCAACGTAACTACCCGCGTCGCGAGGCTCCAGCATTAcc  <  2:237807/151‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                          AAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTCGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACTGCTACGGTTTTCCACTGCGGATTGTTGCTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACC  >  NC_000913/1541290‑1541571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑