Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,593,099	A→G	pseudogene (1027/1461 nt)	yneO ←	pseudogene, AidA homolog

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,593,099	0	A	G	100.0%	62.3 / NA	18	pseudogene (1027/1461 nt)	yneO	pseudogene, AidA homolog
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/9);  total (9/9)

TGCACATTTCCGTCGCCTTCCGTTTCAATGCGCGTTCCGTCTTTCCGGGTATGGTCGGAATCTTTCACACCCATCCAGGTGATTTGCGCCTGTGGCTGGACGTACCAGGTATTCAGCGTCCCTTCGCTGCCGCTAAATGTTCCCGCTTCAAAGGTATACCCACCCTCAACAGAGGCCGTCACACCGCGAGAATCATAGTCGTCAGCAGAACGGTTATCGGAACTGACGCTGTTATCAAACCAGTTATACAGCGCCCAGCTGTCAACATAAGCGCCGGTCTTATT  >  NC_000913/1592963‑1593246                                                                                                                                         |                                                                                                                                                    tGCACATTTCCGTCGCCTTCAGTTTCAATGCGCGTTCCGTCTTTCCGGGTATGGTCGGAATCTTTCACACCCCTCCAGGTGATTTGCGCCTGTGGCTGGACGTACCAGGTATTCAGCGTCCCTTCGCTGCCGCTAAGTGTTCCCGCTTCaa                                                                                                                                       >  2:80858/1‑151 (MQ=255)          ccGTCGCCTTCCGTTTCAATGCGCGTTCCGTCTTTCCGGGTATGGTCGGAATCTTTCACACCCATCCAGGTGATTTGCGCCTGTGGCTGGACGTACCAGGTATTCAGCGTCCCTTCGCTGCCGCTAAGTGTTCCCGCTTCAAAGGTATacc                                                                                                                              <  2:269075/151‑1 (MQ=255)                    ccGTTTCAATGCGCGTTCCGTCTTTCCGGGTATGGTCGGAATCTTTCACACCCATCCAGGTGATTTGCGCCTGTGGCTGGACGTACCAGGTATTCAGCGTCCCTTCGCTGCCGCTAAGTGTTCCCGCTTCAAAGGTATACCCACCCTCAAc                                                                                                                    <  2:189288/151‑1 (MQ=255)                    ccGTTTCAATGCGCGTTCCGTCTTTCCGGGTATGGTCGGAATCTTTCACACCCATCCAGGTGATTTGCGCCTGTGGCTGGACGTACCAGGTATTCAGCGTCCCTTCGCTGCCGCTAAGTGTTCCCGCTTCAAAGGTATACCCACCCTCAAc                                                                                                                    <  2:296688/151‑1 (MQ=255)                       tttCAATGCGCGTTCCGTCTTTCCGGGTATGGTCGGAATCTTTCACACCCATCCAGGTGATTTGCGCCTGTGGCTGGACGTACCAGGTATTCAGCGTCCCTTCGCTGCCGCTAAGTGTTCCCGCTTCAAAGGTATACCCACCCTCAACag                                                                                                                  <  1:99765/150‑1 (MQ=255)                       tttCAATGCGCGTTCCGTCTTTCCGGGTATGGTCGGAATCTTTCACACCCATCCAGGTGATTTGCGCCTGTGGCTGGACGTACCAGGTATTCAGCGTCCCTTCGCTGCCGCTAAGTGTTCCCGCTTCAAAGGTATACCCACCCTCAACag                                                                                                                  <  2:30930/150‑1 (MQ=255)                                                      gTCGGAATCTTTCACACCCATCCAGGTGATTTGCGCCTGTGGCTGGACGTACCAGGTATTCAGCGTCCCTTCGCTGCCGCTAAGTGTTCCCGCTTCAAAGGTATACCCACCCTCAACAGAGGCCGTCACACCGCGAGAATCATAGTCGTca                                                                                  >  2:189676/1‑151 (MQ=255)                                                                      cccATCCAGGTGATTTGCGCCTGTGGCTGGACGTACCAGGTATTCAGCGTCCCTTCGCTGCCGCTAAGTGTTCCCGCTTCAAAGGTATACCCACCCTCAACAGAGGCCGTCACACCGCGAGAATCATAGTCGTCAGCAGAACGGTTATCgg                                                                  >  2:189795/1‑151 (MQ=255)                                                                         aTCCAGGTGATTTGCGCCTGTGGCTGGACGTACCAGGTATTCAGCGTCCCTTCGCTGCCGCTAAGTGTTCCCGCTTCAAAGGTATACCCACCCTCAACAGAGGCCGTCACACCGCGAGAATCATAGTCGTCAGCAGAACGGTTATCGGAAc                                                               <  2:33181/151‑1 (MQ=255)                                                                                         ccTGTGGCTGGACGTACCAGGTATTCAGCGTCCCTTCGCTGCCGCTAAGTGTTCCCGCTTCAAAGGTATACCCACCCTCAACAGAGGCCGTCACACCGCGAGAATCATAGTCGTCAGCAGAACGGTTATCGGAACTGACGCTGTTATCaaa                                               <  2:76398/151‑1 (MQ=255)                                                                                         ccTGTGGCTGGACGTACCAGGTATTCAGCGTCCCTTCGCTGCCGCTAAGTGTTCCCGCTTCAAAGGTATACCCACCCTCAACAGAGGCCGTCACACCGCGAGAATCATAGTCGTCAGCAGAACGGTTATCGGAACTGACGCTGTTATCaaa                                               >  1:289824/1‑151 (MQ=255)                                                                                                         ccAGGTATTCAGCGTCCCTTCGCTGCCGCTAAGTGTTCCCGCTTCAAAGGTATACCCACCCTCAACAGAGGCCGTCACACCGCGAGAATCATAGTCGTCAGCAGAACGGTTATCGGAACTGACGCTGTTATCAAACCAGTTATACAgcgc                                >  2:231484/1‑150 (MQ=255)                                                                                                         ccAGGTATTCAGCGTCCCTTCGCTGCCGCTAAGTGTTCCCGCTTCAAAGGTATACCCACCCTCAACAGAGGCCGTCACACCGCGAGAATCATAGTCGTCAGCAGAACGGTTATCGGAACTGACGCTGTTATCAAACCAGTTATACAgcgc                                >  2:171850/1‑150 (MQ=255)                                                                                                             gTATTCAGCGTCCCTTCGCTGCCGCTAAGTGTTCCCGCTTCAAAGGTATACCCACCCTCAACAGAGGCCGTCACACCGCGAGAATCATAGTCGTCAGCAGAACGGTTATCGGAACTGACGCTGTTATCAAACCAGTTATACAGCGCCCAg                            >  2:310677/1‑150 (MQ=255)                                                                                                               aTTCAGCGTCCCTTCGCTGCCGCTAAGTGTTCCCGCTTCAAAGGTATACCCACCCTCAACAGAGGCCGTCACACCGCGAGAATCATAGTCGTCAGCAGAACGGTTATCGGAACTGACGCTGTTATCAAACCAGTTATACAGCGCCCAg                            >  2:225594/1‑148 (MQ=255)                                                                                                                            tCGCTGCCGCTAAGTGTTCCCGCTTCAAAGGTATACCCACCCTCAACAGAGGCCGTCACACCGCGAGAATCATAGTCGTCAGCAGAACGGTTATCGGAACTGACGCTGTTATCAAACCAGTTATACAGCGCCCAGCTGTCAACATAAgcgc            <  1:274121/151‑1 (MQ=255)                                                                                                                                 gCCGCTAAGTGTTCCCGCTTCAAAGGTATACCCACCCTCAACAGAGGCCGTCACACCGCGAGAATCATAGTCGTCAGCAGAACGGTTATCGGAACTGACGCTGTTATCAAACCAGTTATACAGCGCCCAGCTGTCAACATAAGCGCCGGt        >  1:175474/1‑150 (MQ=255)                                                                                                                                      tAAGTGTTCCCGCTTCAAAGGTATACCCACCCTCAACAGAGGCCGTCACACCGCGAGAATCATAGTCGTCAGCAGAACGGTTATCGGAACTGACGCTGTTATCAAACCAGTTATACAGCGCCCAGCTGTCAACATAAGCGCCGGTCTTAtt  <  2:274343/151‑1 (MQ=255)                                                                                                                                         |                                                                                                                                                    TGCACATTTCCGTCGCCTTCCGTTTCAATGCGCGTTCCGTCTTTCCGGGTATGGTCGGAATCTTTCACACCCATCCAGGTGATTTGCGCCTGTGGCTGGACGTACCAGGTATTCAGCGTCCCTTCGCTGCCGCTAAATGTTCCCGCTTCAAAGGTATACCCACCCTCAACAGAGGCCGTCACACCGCGAGAATCATAGTCGTCAGCAGAACGGTTATCGGAACTGACGCTGTTATCAAACCAGTTATACAGCGCCCAGCTGTCAACATAAGCGCCGGTCTTATT  >  NC_000913/1592963‑1593246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑