Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,659,061	T→G	I331M (ATT→ATG)	ynfE →	putative selenate reductase, periplasmic

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,659,061	0	T	G	100.0%	59.8 / NA	18	I331M (ATT→ATG)	ynfE	putative selenate reductase, periplasmic
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (9/9);  total (9/9)

TATTGCCTGGGTATTGATTAACGAAAATCTCGTTGATCAACCTTTTCTCGATAAATACTGCGTCGGCTATGACGAAAAAACCTTACCCGCAGATGCACCCAAAAATGGTCACTATAAAGCCTATATTCTTGGTGAAGGTGACGATAAAACAGCGAAAACGCCGCAGTGGGCTTCGCAAATTACCGGTATCCCGGAGGACCGTATCATCAAACTGGCGCGTGAAATTGGCACAGCAAAACCCGCTTATATCTGCCAGGGCTGGGGGCCA  >  NC_000913/1658935‑1659202                                                                                                                               |                                                                                                                                              tATTGCCTGGGTATTGATTAACGAAAATCTCGTTGATCAACCTTTTCTCGATAAATACTGCGTCGGCTATGACGAAAAAACCTTACCCGCAGATGCACCCAAAAATGGTCACTATAAAGCCTATATGCTTGGTGAAGGTGACGATAAAAc                                                                                                                        <  2:200456/150‑1 (MQ=255)     gCCTGGGTATTGATTAACGAAAATCTCGTTGATCAACCTTTTCTCGATAAATACTGCGTCGGCTATGACGAAAAAACCTTACCCGCAGATGCACCCAAAAATGGTCACTATAAAGCCTATATGCTTGGTGAAGGTGACGATAAAACAGCGa                                                                                                                   >  2:266236/1‑151 (MQ=255)      ccTGGGTATTGATTAACGAAAATCTCGTTGATCAACCTTTTCTCGATAAATACTGCGTCGGCTATGACGAAAAAACCTTACCCGCAGATGCACCCAAAAATGGTCACTATAAAGCCTATATGCTTGGTGAAGGTGACGATAAAACAGCGaa                                                                                                                  >  1:212660/1‑151 (MQ=255)          ggTATTGATTAACGAAAATCTCGTTGATCAACCTTTTCTCGATAAATACTGCGTCGGCTATGACGAAAAAACCTTACCCGCAGATGCACCCAAAAATGATCACTATAAAGCCTATATGCTTGGTGAAGGTGACGATAAAACAGCGAAAAcg                                                                                                              >  2:165069/1‑151 (MQ=255)                   tAACGAAAATCTCGTTGATCAACCTTTTCTCGATAAATACTGCGTCGGCTATGACGAAAAAACCTTACCCGCAGATGCACCCAAAAATGGTCACTATAAAGCCTATATGCTTGGTGAAGGTGACGATAAAACAGCGAAAACGCCGCAGTgg                                                                                                     <  1:254458/151‑1 (MQ=255)                                         ccTTTTCTCGATAAATACTGCGTCGGCTATGACGAAAAAACCTTACCCGCAGATGCACCCAAAAATGGTCACTATAAAGCCTATATGCTTGGTGAAGGTGACGATAAAACAGCGAAAACGCCGCAGTGGGCTTCGCAAATTACCGGTATcc                                                                               <  2:188127/151‑1 (MQ=255)                                              tctcGATAAATACTGCGTCGGCTATGACGAAAAAACCTTACCCGCAGATGCACCCAAAAATGGTCACTATAAAGCCTATATGCTTGGTGAAGGTGACGATAAAACAGCGAAAACGCCGCAGTGGGCTTCGCAAATTACCGGTATCCCggag                                                                          <  1:231292/151‑1 (MQ=255)                                                   aTAAATACTGCGTCGGCTATGACGAAAAAACCTTACCCGCAGATGCACCCAAAAATGGTCACTATAAAGCCTATATGCTTGGTGAAGGTGACGATAAAACAGCGAAAACGCCGCAGTGGGCTTCGCAAATTACCGGTATCCCGGAGGACCg                                                                     >  2:54100/1‑151 (MQ=255)                                                      aaTACTGCGTCGGCTATGACGAAAAAACCTTACCCGCAGATGCACCCAAAAATGGTCACTATAAAGCCTATATGCTTGGTGAAGGTGACGATAAAACAGCGAAAACGCCGCAGTGGGCTTCGCAAATTACCGGTATCCCGGAGGACCGTat                                                                  <  2:88949/151‑1 (MQ=255)                                                      aaTACTGCGTCGGCTATGACGAAAAAACCTTACCCGCAGATGCACCCAAAAATGGTCACTATAAAGCCTATATGCTTGGTGAAGGTGACGATAAAACAGCGAAAACGCCGCAGTGGGCTTCGCAAATTACCGGTATCCCGGAGGACCGTat                                                                  <  1:12768/151‑1 (MQ=255)                                                      aaTACTGCGTCGGCTATGACGAAAAAACCTTACCCGCAGATGCACCCAAAAATGGTCACTATAAAGCCTATATGCTTGGTGAAGGTGACGATAAAACAGCGAAAACGCCGCAGTGGGCTTCGCAAATTACCGGTATCCCGGAGGACCGTat                                                                  <  1:306159/151‑1 (MQ=255)                                                                         cGAAAAAACCTTACCCGCAGATGCACCCAAAAATGGTCACTATAAAGCCTATATGCTTGGTGAAGGTGACGATAAAACAGCGAAAACGCCGCAGTGGGCTTCGCAAATTACCGGTATCCCGGAGGACCGTATCATCAAACTGGCGCGTGaa                                               >  1:315218/1‑151 (MQ=255)                                                                               aaCCTTACCCGCAGATGCACCCAAAAATGGTCACTATAAAGCCTATATGCTTGGTGAAGGTGACGATAAAACAGCGAAAACGCCGCAGTGGGCTTCGCAAATTACCGGTATCCCGGAGGACCGTATCATCAAACTGGCGCGTGAAATTGGc                                         <  1:37219/151‑1 (MQ=255)                                                                                          cAGATGCACCCAAAAATGGTCACTATAAAGCCTATATGCTTGGTGAAGGTGACGATAAAACAGCGAAAACGCCGCAGTGGGCTTCGCAAATTACCGGTATCCCGGAGGACCGTATCATCAAACTGGCGCGTGAAATTGGCACAGCAAAAc                               <  2:269080/150‑1 (MQ=255)                                                                                                 aCCCAAAAATGGTCACTATAAAGCCTATATGCTTGGTGAAGGTGACGATAAAACAGCGAAAACGCCGCAGTGGGCTTCGCAAATTACCGGTATCCCGGAGGACCGTATCATCAAACTGGCGCGTGAAATTGGCACAGCAAAACCCGCTtat                       >  1:80454/1‑151 (MQ=255)                                                                                                                cTATAAAGCCTATATGCTTGGTGAAGGTGACGATAAAACAGCGAAAACGCCGCAGTGGGCTTCGCAAATTACCGGTATCCCGGAGGACCGTATCATCAAACTGGCGCGTGAAATTGGCACAGCAAAACCCGCTTATATCTGCCAGGGCTgg        >  2:214112/1‑151 (MQ=255)                                                                                                                cTATAAAGCCTATATGCTTGGTGAAGGTGACGATAAAACAGCGAAAACGCCGCAGTGGGCTTCGCAAATTACCGGTATCCCGGAGGACCGTATCATCAAACTGGCGCGTGAAATTGGCACAGCAAAACCCGCTTATATCTGCCAGGGCTgg        >  1:223014/1‑151 (MQ=255)                                                                                                                       gCCTATATGCTTGGTGAAGGTGACGATAAAACAGCGAAAACGCCGCAGTGGGCTTCGCAAATTACCGGTATCCCGGAGGACCGTATCATCAAACTGGCGCGTGAAATTGGCACAGCAAAACCCGCTTATATCTGCCAGGGCTGGGGGCca  >  1:125133/1‑150 (MQ=255)                                                                                                                               |                                                                                                                                              TATTGCCTGGGTATTGATTAACGAAAATCTCGTTGATCAACCTTTTCTCGATAAATACTGCGTCGGCTATGACGAAAAAACCTTACCCGCAGATGCACCCAAAAATGGTCACTATAAAGCCTATATTCTTGGTGAAGGTGACGATAAAACAGCGAAAACGCCGCAGTGGGCTTCGCAAATTACCGGTATCCCGGAGGACCGTATCATCAAACTGGCGCGTGAAATTGGCACAGCAAAACCCGCTTATATCTGCCAGGGCTGGGGGCCA  >  NC_000913/1658935‑1659202

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑