Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	124,558	T→G	D514E (GAT→GAG)	aceE →	pyruvate dehydrogenase, decarboxylase component E1, thiamine triphosphate‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	124,558	0	T	G	100.0%	71.8 / NA	22	D514E (GAT→GAG)	aceE	pyruvate dehydrogenase, decarboxylase component E1, thiamine triphosphate‑binding
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (9/13);  total (9/13)

CAGCCGAACTTCACCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGGCGCGCTGTTGGAAGAGCAGAGCAAAGAGATCTCTACCACTATCGCTTTCGTTCGTGCTCTGAACGTGATGCTGAAGAACAAGTCGATCAAAGATCGTCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTACAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTGCTTACTATAAAGAAGACGAGA  >  NC_000913/124415‑124700                                                                                                                                                |                                                                                                                                               cAGCCGAACTTCACCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGGCGCGCTGTTGGAAGAGCAGAGCAAAGAGATCTCTACCACTATCGCTTTCGTTCGTGCTCTGAACGTGATGCTGAAGAACAAGTCGATCAAAGAGCGTCTgg                                                                                                                                         >  1:3802/1‑151 (MQ=255)    ccGAACTTCACCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGGCGCGCTGTTGGAAGAGCAGAGCAAAGAGATCTCTACCACTATCGCTTTCGTTCGTGCTCTGAACGTGATGCTGAAGAACAAGTCGATCAAAGAGCGTCTGGTAc                                                                                                                                      <  1:97602/151‑1 (MQ=255)         cTTCACCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGGCGCGCTGTTGGAAGAGCAGAGCAAAGAGATCTCTACCACTATCGCTTTCGTTCGTGCTCTGAACGTGATGCTGAAGAACAAGTCGATCAAAGAGCGTCTGGTACCGatc                                                                                                                                 <  1:265294/151‑1 (MQ=255)                  gaAGCTTGAGCTGCCGAGCCTGCAAGACTTCGGCGCGCTGTTGGAAGAGCAGAGCAAAGAGATCTCTACCACTATCGCTTTCGTTCGTGCTCTGAACGTGATGCTGAAGAACAAGTCGATCAAAGAGCGTCTGGTACCGATCATCGCcga                                                                                                                         >  2:301641/1‑150 (MQ=255)                        tGAGCTGCCGAGCCTGCAAGACTTCGGCGCGCTGTTGGAAGAGCAGAGCAAAGAGATCTCTACCACTATCGCTTTCGTTCGTGCTCTGAACGTGATGCTGAAGAACAAGTCGATCAAAGAGCGTCTGGTACCGATCATCGCCGACGAAgcg                                                                                                                  <  1:90896/151‑1 (MQ=255)                                         aaGACTTCGGCGCGCTGTTGGAAGAGCAGAGCAAAGAGATCTCTACCACTATCGCTTTCGTTCGTGCTCTGAACGTGATGCTGAAGAACAAGTCGATCAAAGAGCGTCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTATGGa                                                                                                 <  2:278509/151‑1 (MQ=255)                                                   cgcgcTGTTGTAATAGCAAAGCAAAGATACCTCGACGAGTATCACTTTCGTTCGTGCTCGGAACGTGATGGCGAAGAACAAGTCTATCGTAGAGCGTCTGGTACCGACCATCGCCGATGAAGCGCTTACTTTCGGTATGGAAGGTCTGTTc                                                                                       <  2:80551/151‑1 (MQ=255)                                                         gTTGGAAGAGCAGAGCAAAGAGATCTCTACCACTATCGCTTTCGTTCGTGCTCTGAACGTGATGCTGAAGAACAAGTCGATCAAAGAGCGTCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTATGGAAGGTCTGTTCCGTCAg                                                                                 >  2:70872/1‑151 (MQ=255)                                                                 agcagagcaAAGAGATCTCTACCACTATCGCTTTCGTTCGTGCTCTGAACGTGATGCTGAAGAACAAGTCGATCAAAGAGCGTCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTa                                                                          <  1:80971/150‑1 (MQ=255)                                                                     gagcaAAGAGATCTCTACCACTATCGCTTTCGTTCGTGCTCTGAACGTGATGCTGAAGAACAAGTCGATCAAAGAGCGTCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTa                                                                      >  2:294155/1‑150 (MQ=255)                                                                      agcaAAGAGATCTCTACCACTATCGCTTTCGTTCGTGCTCTGAACGTGATGCTGAAGAACAAGTCGATCAAAGAGCGTCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTAc                                                                     <  1:102456/150‑1 (MQ=255)                                                                               aTCTCTACCACTATCGCTTTCGTTCGTGCTCTGAACGTGATGCTGAAGAACAAGTCGATCAAAGAGCGTCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTACAGCCCGAAc                                                            <  1:171636/150‑1 (MQ=255)                                                                                        aCTATCGCTTTCGTTCGTGCTCTGAACGTGATGCTGAAGAACAAGTCGATCAAAGAGCGTCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTACAGCCCGAACGGTCAGCAGt                                                  <  2:42938/151‑1 (MQ=255)                                                                                        aCTATCGCTTTCGTTCGTGCTCTGAACGTGATGCTGAAGAACAAGTCGATCAAAGAGCGTCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTACAGCCCGAACGGTCAGCAGt                                                  <  2:222448/151‑1 (MQ=255)                                                                                            tCGCTTTCGTTCGTGCTCTGAACGTGATGCTGAAGAACAAGTCGATCAAAGAGCGTCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTACAGCCCGAACGGTCAGCAGTacac                                              <  1:85124/151‑1 (MQ=255)                                                                                               cTTTCGTTCGTGCTCTGAACGTGATGCTGAAGAACAAGTCGATCAAAGAGCGTCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTACAGCCCGAACGGTCAGCAGTACAcccc                                           <  1:257813/151‑1 (MQ=255)                                                                                                      tcgtGCTCTGAACGTGATGCTGAAGAACAAGTCGATCAAAGAGCGTCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTACAGCCCGAACGGTCAGCAGTACACCCCGCAGGAc                                    <  1:238368/151‑1 (MQ=255)                                                                                                           ctctGAACGTGATGCTGAAGAACAAGTCGATCAAAGAGCGTCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTACAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGa                               >  1:41731/1‑151 (MQ=255)                                                                                                                         cTGAAGAACAAGTCGATCAAAGAGCGTCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTACAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTGCTTa                   >  2:33619/1‑149 (MQ=255)                                                                                                                                  aaGTCGATCAAAGAGCGTCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTACAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTGCTTACTATAaagaag        >  2:264029/1‑151 (MQ=255)                                                                                                                                        aTCAAAGAGCGTCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTACAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTGCTTACTATAAAGAAGACgaga  >  2:51440/1‑151 (MQ=255)                                                                                                                                        aTCAAAGAGCGTCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTACAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTGCTTACTATAAAGAAGACgaga  >  2:890/1‑151 (MQ=255)                                                                                                                                                |                                                                                                                                               CAGCCGAACTTCACCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGGCGCGCTGTTGGAAGAGCAGAGCAAAGAGATCTCTACCACTATCGCTTTCGTTCGTGCTCTGAACGTGATGCTGAAGAACAAGTCGATCAAAGATCGTCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTACAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTGCTTACTATAAAGAAGACGAGA  >  NC_000913/124415‑124700

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑