Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,061,032	C→T	W301* (TGG→TAG)	nac ←	nitrogen assimilation regulon transcriptional regulator; autorepressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,061,032	0	C	T	100.0%	59.6 / NA	17	W301* (TGG→TAG)	nac	nitrogen assimilation regulon transcriptional regulator; autorepressor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/10);  total (7/10)

TTTAGTTGTTGGAAATTCACGGTAAACTCCGGGCAGTTCAGATTTCCCGTTATTGTTAAAGTCTAATGCCCGGCATAACAAATAATAAAAACCCGCATCTTATTCCATCCCGATATAACACTTAGCTCACCAATTGCCACTGCCTTTTTTCCATCACTGGAGAACTAATCACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAGGAGTGGT  >  NC_000913/2060895‑2061162                                                                                                                                          |                                                                                                                                   tttAGTTGTTGGAAATTCACGGTAAACTCCGGGCAGTTCAGATTTCCCGTTATTGTTAAAGTCTAATGCCCGGCATAACAAATAATAAAAACCCGCATCTTATTCCATCCCGATATAACACTTAGCTCACCAATTGCTACTGCCTTTTTTc                                                                                                                       <  1:36555/151‑1 (MQ=255)                                     ttCAGATTTCCCGTTATTGTTAAAGTCTAATGCCCGGCATAACAAATAATAAAAACCCGCATCTTATTCCATCCCGATATAACACTTAGCTCACCAATTGCTACTGCCTTTTTTCCATCACTGGAGAACTAATCACTGACATTAACAGctc                                                                                   <  1:57601/151‑1 (MQ=255)                                     ttCAGATTTCCCGTTATTGTTAAAGTCTAATGCCCGGCATAACAAATAATAAAAACCCGCATCTTATTCCATCCCGATATAACACTTAGCTCACCAATTGCTACTGCCTTTTTTCCATCACTGGAGAACTAATCACTGACATTAACAActc                                                                                   <  1:184715/151‑1 (MQ=255)                                     ttCAGATTTCCCGTTATTGTTAAAGTCTAATGCCCGGCATAACAAATAATAAAAACCCGCATCTTATTCCATCCCGATATAACACTTAGCTCACCAATTGCTACTGCCTTTTTTCCATCACTGGAGAACTAATCACTGACATTAACAActc                                                                                   <  2:138094/151‑1 (MQ=255)                                     ttCAGATTTCCCGTTATTGTTAAAGTCTAATGCCCGGCATAACAAATAATAAAAACCCGCATCTTATTCCATCCCGATATAACACTTAGCTCACCAATTGCTACTGCCTTTTTTCCATCACTGGAGAACTAATCACTGACATTAACAAct                                                                                    <  1:104566/150‑1 (MQ=255)                                     ttCAGATTTCCCGTTATTGTTAAAGTCTAATGCCCGGCATAACAAATAATAAAAACCCGCATCTTATTCCATCCCGATATAACACTTAGCTCACCAATTGCTACTGCCTTTTTTCCATCACTGGAGAACTAATCACTGACATTAACAAct                                                                                    <  1:51787/150‑1 (MQ=255)                                                     ttGTTAAAGTCTAATGCCCGGCATAACAAATAATAAAAACCCGCATCTTATTCCATCCCGATATAACACTTAGCTCACCAATTGCTACTTCCTTTTTTCCATCACTGGAGAACTAATCACTGACATTAACAACTCTTTCACtgcctgtgcc                                                                   <  2:51338/151‑1 (MQ=255)                                                             gTCTAATGCCCGGCATAACAAATAATAAAAACCCGCATCTTATTCCATCCCGATATAACACTTAGCTCACCAATTGCTACTGCCTTTTTTCCATCACTGGAGAACTAATCACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGCGa                                                           >  1:238041/1‑151 (MQ=255)                                                                         gCATAACAAATAATAAAAACCCGCATCTTATTCCATCCCGATATAACACTTAGCTCACCAATTGCTACTGCCTTTTTTCCATCACTGGAGAACTAATCACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGCGATAAGTTCGct                                                 >  1:125618/1‑149 (MQ=255)                                                                         gCATAACAAATAATAAAAACCCGCATCTTATTCCATCCCGATATAACACTTAGCTCACCAATTGCTACTGCCTTTTTTCCATCACTGGAGAACTAATCACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGCGATAAGTTCGct                                                 >  2:197373/1‑149 (MQ=255)                                                                                                 aTCTTATTCCATCCCGATATAACACTTAGCTCACCAATTGCTACTGCCTTTTTTCCATCACTGGAGAACTAATCACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAaga                       >  1:151532/1‑151 (MQ=255)                                                                                                 aTCTTATTCCATCCCGATATAACACTTAGCTCACCAATTGCTACTGCCTTTTTTCCATCACTGGAGAACTAATCACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAaga                       >  1:51803/1‑151 (MQ=255)                                                                                                       ttCCATCCCGATATAACACTTAGCTCACCAATTGCTACTGCCTTTTTTCCATCACTGGAGAACTAATCACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTGAATTTAATGACAAAGAGAGACt                 <  1:243325/151‑1 (MQ=255)                                                                                                              ccGATATAACACTTAGCTCACCAATTGCTACTGCCTTTTTTCCATCACTGGAGAACTAATCACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGaa          <  1:137520/151‑1 (MQ=255)                                                                                                                 atatAACACTTAGCTCACCAATTGCTACTGCCTTTTTTCCATCACTGGAGAACTAATCACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAGGa       >  1:189803/1‑151 (MQ=255)                                                                                                                  tataACACTTAGCTCACCAATTGCTACTGCCTTTTTTCCATCACTGGAGAACTAATCACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAGGAg      <  1:230725/151‑1 (MQ=255)                                                                                                                      acacTTAGCTCACCAATTGCTACTGCCTTTTTTCCATCACTGGAGAACTAATCACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAGGAGTGGt  >  2:90118/1‑151 (MQ=255)                                                                                                                                          |                                                                                                                                   TTTAGTTGTTGGAAATTCACGGTAAACTCCGGGCAGTTCAGATTTCCCGTTATTGTTAAAGTCTAATGCCCGGCATAACAAATAATAAAAACCCGCATCTTATTCCATCCCGATATAACACTTAGCTCACCAATTGCCACTGCCTTTTTTCCATCACTGGAGAACTAATCACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAGGAGTGGT  >  NC_000913/2060895‑2061162

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑