Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,143,783	T→G	L173R (CTG→CGG)	yegE →	putative diguanylate cyclase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,143,783	0	T	G	100.0%	37.4 / NA	12	L173R (CTG→CGG)	yegE	putative diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (5/7);  total (5/7)

CGGCAGCGCCATTGTTCCGCCTCTGTT‑GGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGTTATTTAAACCACACTATCTGCTGCGCCATCGCAACCCACGGTTGCTTTTTGAGTCGCTGCTCACGTTAGCCATCACACTGACGTTAAGCTGGCTTTCGATGCTGTATCTGCCGTGGCCTTTTACTTTCATTATTG  >  NC_000913/2143652‑2143926                                                                                                                                     |                                                                                                                                                cGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCGGGGATTGTTATTTAAAc                                                                                                                                >  2:163518/1‑150 (MQ=255)       cgcCATTGTTCCGCCTCTGTT‑GGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCGGGGATTGTTATTTAAACCacac                                                                                                                           <  2:294476/148‑1 (MQ=255)          cATTGTTCCGCCTCTGTT‑GGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCGGGGATTGTTATTTAAACCACACTATctg                                                                                                                     <  2:14366/151‑1 (MQ=255)                       ctGTT‑GGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCGGGGATTGTTATTTAAACCACACTATCTGCTGCGCCATCGCa                                                                                                        >  2:213153/1‑151 (MQ=255)                          tt‑GGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCGGGGATTGTTATTTAAACCACACTATCTGCTGCGCCATCGCAAcc                                                                                                     <  1:66620/151‑1 (MQ=255)                                                         cccGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCGGGGATTGTTATTTAAACCACACTATCTGCTGCGCCATCGCAACCCACGGTTGCTTTTTGAGTCGCTGCTCACGt                                                                       >  2:21943/1‑151 (MQ=255)                                                                   aTCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCGGGGATTGTTATTTAAACCACACTATCTGCTGCGCCATCGCAACCCACGGTTGCTTTTTGAGTCGCTGCTCACGTTAGCCATcac                                                             <  2:146779/151‑1 (MQ=255)                                                                                aTTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCGGGGATTGTTATTTAAACCACACTATCTGCTGCGCCATCGCAACCCACGGTTGCTTTTTGAGTCGCTGCTCACGTTAGCCATCACACTGACGTTAAg                                                 >  1:121323/1‑150 (MQ=255)                                                                                    ttGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCGGGGATTGTTATTTAAACCACACTATCTGCTGCGCCATCGCAACCCACGGTTGCTTTTTGAGTCGCTGCTCACGTTAGCCATCACACTGACGTTAAGCTGGc                                            <  2:265910/151‑1 (MQ=255)                                                                                           gggTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCGGGGATTGTTATTTAAACCACACTATCTGCTGCGCCATCGCAACCCACGGTTGCTTTTTGAGTCGCTGCTCACGTTAGCCATCACACTGACGTTAAGCTGGCTTTCGAt                                     >  2:47794/1‑151 (MQ=255)                                                                                                       aaTCCATCGGCGCTCTGGCACTGGTGCCGCGGGGATTGTTATTTAAACCACACTATCTGCTGCGCCATCGCAACCCACGGTTGCTTTTTGAGTCGCTGCTCACGTTAGCCATCACACTGACGTTAAGCTGGCTTTCGATGCTGTATCTGcc                         <  2:133828/151‑1 (MQ=255)                                                                                                                               tGCCGCGGGGATTGTTATTTAAACCACACTATCTGCTGCGCCATCGCAACCCACGGTTGCTTTTTGAGTCGCTGCTCACGTTAGCCATCACACTGACGTTAAGCTGGCTTTCGATGCTGTATCTGCCGTGGCCTTTTACTTTCATTATtg  <  2:304062/150‑1 (MQ=255)                                                                                                                                     |                                                                                                                                                CGGCAGCGCCATTGTTCCGCCTCTGTT‑GGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGTTATTTAAACCACACTATCTGCTGCGCCATCGCAACCCACGGTTGCTTTTTGAGTCGCTGCTCACGTTAGCCATCACACTGACGTTAAGCTGGCTTTCGATGCTGTATCTGCCGTGGCCTTTTACTTTCATTATTG  >  NC_000913/2143652‑2143926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑