Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,259,179	C→T	G40S (GGT→AGT)	psuK ←	pseudouridine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,259,179	0	C	T	100.0%	64.5 / NA	18	G40S (GGT→AGT)	psuK	pseudouridine kinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/7);  total (11/7)

CAATCAGGCATTTATCGACATAAACGCCAGATTGATTGGTTTGCGTTAGCAGCGATTGACCATAAAAATCACTGCCTACGGCGCTCAGTAGCCAGGCTTTGTTACCCAGCAACGCCAGGTTTTGTGCAATATTGCGCCCTACTCCACCAGGCGTAAATTTTATTTTACCTGGATTTGAATCCGCATAATTTAATGATTCATGTGAATATCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTAC  >  NC_000913/2259032‑2259321                                                                                                                                                    |                                                                                                                                               caATCAGGCATTTATCGACATAAACGCCAGATTGATTGGTTTGCGTTAGCAGCGATTGACCATAAAAATCACTGCCTACGGCGCTCAGTAGCCAGGCTTTGTTACCCAGCAACGCCAGGTTTTGTGCAATATTGCGCCCTACTCCACTAgg                                                                                                                                             <  2:1258/151‑1 (MQ=255)                                         ttGCGTTAGCAGCGATTGACCATAAAAATCACTGCCTACGGCGCTCAGTAGCCAGGCTTTGTTACCCAGCAACGCCAGGTTTTGTGCAATATTGCGCCCTACTCCACTAGGCGTAAATTTTATTTTACCTGGATTTGAATCCGCATAAttt                                                                                                     <  2:7283/151‑1 (MQ=255)                                              ttAGCAGCGATTGACCATAAAAATCACTGCCTACGGCGCTCAGTAGCCAGGCTTTGTTACCCAGCAACGCCAGGTTTTGTGCAATATTGCGCCCTACTCCACTAGGCGTAAATTTTATTTTACCTGGATTTGAATCCGCATAATTTAATGa                                                                                                <  1:283370/151‑1 (MQ=255)                                                                                   gcTCAGTAGCCAGGCTTTGTTACCCAGCAACGCCAGGTTTTGTGCAATATTGCGCCCTACTCCACTAGGCGTAAATTTTATTTTACCTGGATTTGAATCCGCATAATTTAATGATTCATGTGAATATCCGGCGACATCAATATTCGCCGaa                                                           >  2:79736/1‑151 (MQ=255)                                                                                               ggCTTTGTTACCCAGCAACGCCAGGTTTTGTGCAATATTGCGCCCTACTCCACTAGGCGTAAATTTTATTTTACCTGGATTTGAATCCGCATAATTTAATGATTCATGTGAATATCCGGCGACATCAATATTCGCCGAACCTATAATTACg                                               >  1:202323/1‑151 (MQ=255)                                                                                                   ttGTTACCCAGCAACGCCAGGTTTTGTGCAATATTGCGCCCTACTCCACTAGGCGTAAATTTTATTTTACCTGGATTTGAATCCGCATAATTTAATGATTCATGTGAATATCCGGCGACATCAATATTCGCCGAACCTATAATTACGACAt                                           >  2:94035/1‑151 (MQ=255)                                                                                                   ttGTTACCCAGCAACGCCAGGTTTTGTGCAATATTGCGCCCTACTCCACTAGGCGTAAATTTTATTTTACCTGGATTTGAATCCGCATAATTTAATGATTCATGTGAATATCCGGCGACATCAATATTCGCCGAACCTATAATTACGACAt                                           >  1:275581/1‑151 (MQ=255)                                                                                                        aCCCAGCAACGCCAGGTTTTGTGCAATATTGCGCCCTACTCCACTAGGCGTAAATTTTATTTTACCTGGATTTGAATCCGCATAATTTAATGATTCATGTGAATATCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATcc                                      <  2:216955/151‑1 (MQ=255)                                                                                                        aCCCAGCAACGCCAGGTTTTGTGCAATATTGCGCCCTACTCCACTAGGCGTAAATTTTATTTTACCTGGATTTGAATCCGCATAATTTAATGATTCATGTGAATATCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATcc                                      <  1:189070/151‑1 (MQ=255)                                                                                                        aCCCAGCAACGCCAGGTTTTGTGCAATATTGCGCCCTACTCCACTAGGCGTAAATTTTATTTTACCTGGATTTGAATCCGCATAATTTAATGATTCATGTGAATATCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATcc                                      <  1:11138/151‑1 (MQ=255)                                                                                                                   ccAGGTTTTGTGCAATATTGCGCCCTACTCCACTAGGCGTAAATTTTATTTTACCTGGATTTGAATCCGCATAATTTAATGATTCATGTGAATATCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCAt                           <  1:148327/151‑1 (MQ=255)                                                                                                                            gtgCAATATTGCGCCCTACTCCACTAGGCGTAAATTTTATTTTACCTGGATTTGAATCCGCATAATTTAATGATTCATGTGAATATCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTc                   >  1:117498/1‑150 (MQ=255)                                                                                                                              gcgatatTGCGCCCTACTCCACTAGGCGTAAATTTTATTTTACCTGGATTTGAATCCGCATAATTTAATGATTCATGTGAATATCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCct                 >  1:132102/4‑150 (MQ=255)                                                                                                                                 atatTGCGCCCTACTCCACTAGGCGTAAATTTTATTTTACCTGGATTTGAATCCGCATAATTTAATGATTCATGTGAATATCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTgg             >  1:6532/1‑151 (MQ=255)                                                                                                                                             aCTCCACTAGGCGTAAATTTTATTTTACCTGGATTTGAATCCGCATAATTTAATGATTCATGTGAATATCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTAc  >  1:43041/1‑150 (MQ=255)                                                                                                                                             aCTCCACTAGGCGTAAATTTTATTTTACCTGGATTTGAATCCGCATAATTTAATGATTCATGTGAATATCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTAc  >  1:143152/1‑150 (MQ=255)                                                                                                                                             aCTCCACTAGGCGTAAATTTTATTTTACCTGGATTTGAATCCGCATAATTTAATGATTCATGTGAATATCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTAc  >  2:51602/1‑150 (MQ=255)                                                                                                                                             aCTCCACTAGGCGTAAATTTTATTTTACCTGGATTTGAATCCGCATAATTTAATGATTCATGTGAATATCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTAc  >  2:64795/1‑150 (MQ=255)                                                                                                                                                    |                                                                                                                                               CAATCAGGCATTTATCGACATAAACGCCAGATTGATTGGTTTGCGTTAGCAGCGATTGACCATAAAAATCACTGCCTACGGCGCTCAGTAGCCAGGCTTTGTTACCCAGCAACGCCAGGTTTTGTGCAATATTGCGCCCTACTCCACCAGGCGTAAATTTTATTTTACCTGGATTTGAATCCGCATAATTTAATGATTCATGTGAATATCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTAC  >  NC_000913/2259032‑2259321

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑