Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,562,066	A→G	intergenic (+73/‑45)	yffN → / → yffO	CPZ‑55 prophage; uncharacterized protein/CPZ‑55 prophage; uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,562,066	0	A	G	100.0%	50.0 / NA	16	intergenic (+73/‑45)	yffN/yffO	CPZ‑55 prophage; uncharacterized protein/CPZ‑55 prophage; uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (8/8);  total (8/8)

GAAATGGTTCACGAAATCATAAGAATTGCTAGAGATTTGGAAGCGCCAGTTGACGCTCCGTTAGATTGCTAACGCCAAAATTAGCGCTGAATTAATGGGTAATTATTGCGGTTTTTGTCAATTTTCAACCTCAATTCTTGCGAAAAATGGCAAAAATAACGGTATTTTTCATCATTTATTGGGGGTTTTATGGAAGGCGAACTGATAGAAAACAATGGGTTAGACATTTATGACACCAGCGAAACACCGAAAAAGCGCGGTCGGCCAGCTAAATATAATGAAAAAAT  >  NC_000913/2561922‑2562208                                                                                                                                                 |                                                                                                                                               gAAATGGTTCACGAAATCATAAGAATTGCTAGAGATTTGGAAGCGCCAGTTGACGCTCCGTTAGATTGCTAACGCCAAAATTAGCGCTGAATTAATGGGTAATTATTGCGGTTTTTGTCAATTTTCAACCTCAATTCTTGCGAAGAATgg                                                                                                                                           >  1:210794/1‑150 (MQ=255)                aTCATAAGAATTGCTAGAGATTTGGAAGCGCCAGTTGACGCTCCGTTAGATTGCTAACGCCAAAATTAGCGCTGAATTAATGGGTAATTATTGCGGTTTTTGTCAATTTTCAACCTCAATTCTTGCGAAGAATGGCAAAAATAACGGTAtt                                                                                                                           >  1:272581/1‑151 (MQ=255)                                                     aCGCTCCGTTAGATTGCTAACGCCAAAATTAGCGCTGAATTAATGGGTAATTATTGCGGTTTTTGTCAATTTTCAACCTCAATTCTTGCGAAGAATGGCAAAAATAACGGTATTTTTCATCATTTATTGGGGGTTTTATGGAAGGCGAAc                                                                                       <  2:110943/150‑1 (MQ=255)                                                                       aaCGCCAAAATTAGCGCTGAATTAATGGGTAATTATTGCGGTTTTTGTCAATTTTCAACCTCAATTCTTGCGAAGAATGGCAAAAATAACGGTATTTTTCATCATTTATTGGGGGTTTTATGGAAGGCGAACTGATAGAAAACAATGGGtt                                                                    <  2:118679/151‑1 (MQ=255)                                                                           ccAAAATTAGCGCTGAATTAATGGGTAATTATTGCGGTTTTTGTCAATTTTCAACCTCAATTCTTGCGAAGAATGGCAAAAATAACGGTATTTTTCATCATTTATTGGGGGTTTTATGGAAGGCGAACTGATAGAAAACAATGGGTTAGAc                                                                <  1:309742/151‑1 (MQ=255)                                                                            cAAAATTAGCGCTGAATTAATGGGTAATTATTGCGGTTTTTGTCAATTTTCAACCTCAATTCTTGCGAAGAATGGCAAAAATAACGGTATTTTTCATCATTTATTGGGGGTTTTATGGAAGGCGAACTGATAGAAAACAATGGGTTAGACa                                                               <  1:53308/151‑1 (MQ=255)                                                                             aaaaTTAGCGCTGAATTAATGGGTAATTATTGCGGTTTTTGTCAATTTTCAACCTCAATTCTTGCGAAGTATTGCAAAAATAACTGTATTTTTCATCATTTATTGGGAGTTTTATGGAAGGCGAACTGATATAAAACAATGGGTTAGACAt                                                              <  2:146261/151‑1 (MQ=255)                                                                                 ttAGCGCTGAATTAATGGGTAATTATTGCGGTTTTTGTCAATTTTCAACCTCAATTCTTGCGAAGAATGGCAAAAATAACGGTATTTTTCATCATTTATTGGGGGTTTTATGGAAGGCGAACTGATAGAAAACAATGGGTTAGACATTTAt                                                          <  2:226508/151‑1 (MQ=255)                                                                                         gAATTAATGGGTAATTATTGCGGTTTTTGTCAATTTTCAACCTCAATTCTTGCGAAGAATGGCAAAAATAACGGTATTTTTCATCATTTATTGGGGGTTTTATGGAAGGCGAACTGATAGAAAACAATGGGTTAGACATTTATGACACCAg                                                  >  1:173134/1‑151 (MQ=255)                                                                                         gAATTAATGGGTAATTATTGCGGTTTTTGTCAATTTTCAACCTCAATTCTTGCGAAGAATGGCAAAAATAACGGTATTTTTCATCATTTATTGGGGGTTTTATGGAAGGCGAACTGATAGAAAACAATGGGTTAGACATTTATGACACCAg                                                  >  2:318700/1‑151 (MQ=255)                                                                                         gAATTAATGGGTAATTATTGCGGTTTTTGTCAATTTTCAACCTCAATTCTTGCGAAGAATGGCAAAAATAACGGTATTTTTCATCATTTATTGGGGGTTTTATGGAAGGCGAACTGATAGAAAACAATGGGTTAGACATTTATGACACCAg                                                  >  2:59509/1‑151 (MQ=255)                                                                                                       ttattGCGGTTTTTGTCAATTTTCAACCTCAATTCTTGCGAAGAATGGCAAAAATAACGGTATTTTTCATCATTTATTGGGGGTTTTATGGAAGGCGAACTGATAGAAAACAATGGGTTAGACATTTATGACACCAGCGAAACACCGaaaa                                    >  2:304234/1‑151 (MQ=255)                                                                                                                              cAACCTCAATTCTTGCGAAGAATGGCAAAAATAACGGTATTTTTCATCATTTATTGGGGGTTTTATGGAAGGCGAACTGATAGAAAACAATGGGTTAGACATTTATGACACCAGCGAAACACCGAAAAAGCGCGGTCGGCCAGCTAAatat             <  2:119682/151‑1 (MQ=255)                                                                                                                              cAACCTCAATTCTTGCGAAGAATGGCAAAAATAACGGTATTTTTCATCATTTATTGGGGGTTTTATGGAAGGCGAACTGATAGAAAACAATGGGTTAGACATTTATGACACCAGCGAAACACCGAAAAAGCGCGGTCGGCCAGCTAAatat             <  2:4383/151‑1 (MQ=255)                                                                                                                                  cTCAATTCTTGCGAAGAATGGCAAAAATAACGGTATTTTTCATCATTTATTGGGGGTTTTATGGAAGGCGAACTGATAGAAAACAATGGGTTAGACATTTATGACACCAGCGAAACACCGAAAAAGCGCGGTCGGCCAGCTAAATATAATg         >  1:295559/1‑151 (MQ=255)                                                                                                                                         cTTGCGAAGAATGGCAAAAATAACGGTATTTTTCATCATTTATTGGGGGTTTTATGGAAGGCGAACTGATAGAAAACAATGGGTTAGACATTTATGACACCAGCGAAACACCGAAAAAGCGCGGTCGGCCAGCTAAATATAATGAAAAAAt  >  2:237349/1‑151 (MQ=255)                                                                                                                                                 |                                                                                                                                               GAAATGGTTCACGAAATCATAAGAATTGCTAGAGATTTGGAAGCGCCAGTTGACGCTCCGTTAGATTGCTAACGCCAAAATTAGCGCTGAATTAATGGGTAATTATTGCGGTTTTTGTCAATTTTCAACCTCAATTCTTGCGAAAAATGGCAAAAATAACGGTATTTTTCATCATTTATTGGGGGTTTTATGGAAGGCGAACTGATAGAAAACAATGGGTTAGACATTTATGACACCAGCGAAACACCGAAAAAGCGCGGTCGGCCAGCTAAATATAATGAAAAAAT  >  NC_000913/2561922‑2562208

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑