Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,612,344	A→C	K149Q (AAA→CAA)	hyfR →	hydrogenase‑4 transcriptional activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,612,344	0	A	C	100.0%	85.9 / NA	24	K149Q (AAA→CAA)	hyfR	hydrogenase‑4 transcriptional activator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (11/13);  total (11/13)

CACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGTTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGAAAGATCGCGATCATCATCGGGTACTGGTTGATATTACTAATGCAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCAT  >  NC_000913/2612217‑2612490                                                                                                                                |                                                                                                                                                   cACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGTTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGCAAGATCGCGATCATCATCGGGTa                                                                                                                             >  2:129320/1‑151 (MQ=255)     cACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGTTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGCAAGATCGCGATCATCATCGGGTACTgg                                                                                                                         >  1:195075/1‑151 (MQ=255)      aCGGCAGTCGTTTTACTTCCCAGGATTGCACACTGTTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGCAAGATCGCGATCATCATCGGGTACTGGt                                                                                                                        <  2:87748/151‑1 (MQ=255)             tCGTTTTACTTCCCAGGATTGCACACTGTTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGCAAGATCGCGATCATCATCGGGTACTGGTTGatat                                                                                                                  <  1:78266/150‑1 (MQ=255)                 tttACTTCCCAGGATTGCACACTGTTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGCAAGATCGCGATCATCATCGGGTACTGGTTGATATTACTa                                                                                                             <  1:8228/151‑1 (MQ=255)                     cTTCCCAGGATTGCACACTGTTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGCAAGATCGCGATCATCATCGGGTACTGGTTGATATTACTAATGc                                                                                                         <  2:225164/151‑1 (MQ=255)                     cTTCCCAGGATTGCACACTGTTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGCAAGATCGCGATCATCATCGGGTACTGGTTGATATTACTAATGc                                                                                                         <  1:25298/151‑1 (MQ=255)                             gATTGCACACTGTTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGCAAGATCGCGATCATCATCGGGTACTGGTTGATATTACTAATGCAGTg                                                                                                     >  1:219440/1‑147 (MQ=255)                               ttGCACACTGTTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGCAAGATCGCGATCATCATCGGGTACTGGTTGATATTACTAATGCAGTGCTGTca                                                                                               <  1:246271/151‑1 (MQ=255)                                  cacaCTGTTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGCAAGATCGCGATCATCATCGGGTACTGGTTGATATTACTAATGCAGTGCTGTcaca                                                                                             <  1:150367/150‑1 (MQ=255)                                      cTGTTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGCAAGATCGCGATCATCATCGGGTACTGGTTGATATTACTAATGCAGTGCTGTCACATCTTg                                                                                        >  2:200810/1‑151 (MQ=255)                                      cTGTTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGCAAGATCGCGATCATCATCGGGTACTGGTTGATATTACTAATGCAGTGCTGTCACATCTTg                                                                                        >  1:281653/1‑151 (MQ=255)                                        gTTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGCAAGATCGCGATCATCATCGGGTACTGGTTGATATTACTAATGCAGTGCTGTCACATCTTGAt                                                                                      >  1:276520/1‑151 (MQ=255)                                        gTTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGCAAGATCGCGATCATCATCGGGTACTGGTTGATATTACTAATGCAGTGCTGTCACATCTTGAt                                                                                      >  1:233867/1‑151 (MQ=255)                                         tttAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGCAAGATCGCGATCATCATCGGGTACTGGTTGATATTACTAATGCAGTGCTGTCACATCTTGAt                                                                                      <  1:123403/150‑1 (MQ=255)                                                        cgGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGCAAGATCGCGATCATCATCGGGTACTGGTTGATATTACTAATGCAGTGCTGTCACATCTTGATCTCGACGATCTGATCg                                                                      >  2:232699/1‑151 (MQ=255)                                                                       gTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGCAAGATCGCGATCATCATCGGGTACTGGTTGATATTACTAATGCAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTg                                                       >  2:143342/1‑151 (MQ=255)                                                                        ttCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGCAAGATCGCGATCATCATCGGGTACTGGTTGATATTACTAATGCAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTg                                                       <  1:143343/150‑1 (MQ=255)                                                                                        cAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGCAAGATCGCGATCATCATCGGGTACTGGTTGATATTACTAATGCAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCAtttttt                                       >  2:91178/1‑150 (MQ=255)                                                                                                              gcgcTGCGTTTGCTGGAGCAAGATCGCGATCATCATCGGGTACTGGTTGATATTACTAATGCAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATgg                >  2:129289/1‑151 (MQ=255)                                                                                                               cgcTGCGTTTGCTGGAGCAAGATCGCGATCATCATCGGGTACTGGTTGATATTACTAATGCAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGt               <  1:266665/151‑1 (MQ=255)                                                                                                                 cTGCGTTTGCTGGAGCAAGATCGCGATCATCATCGGGTACTGGTTGATATTACTAATGCAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTAc             <  2:16324/151‑1 (MQ=255)                                                                                                                 cTGCGTTTGCTGGAGCAAGATCGCGATCATCATCGGGTACTGGTTGATATTACTAATGCAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTAc             <  2:86855/151‑1 (MQ=255)                                                                                                                 cTGCGTTTGCTGGAGCAAGATCGCGATCATCATCGGGTACTGGTTGATATTACTAATGCAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTAc             <  1:187018/151‑1 (MQ=255)                                                                                                                             gagcAAGATCGCGATCATCATCGGGTACTGGTTGATATTACTAATGCAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGatcat  >  1:63133/1‑150 (MQ=255)                                                                                                                                |                                                                                                                                                   CACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGTTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGAAAGATCGCGATCATCATCGGGTACTGGTTGATATTACTAATGCAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCAT  >  NC_000913/2612217‑2612490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑