Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,697,273	T→G	F491L (TTT→TTG)	mltF →	membrane‑bound lytic transglycosylase F, murein hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,697,273	0	T	G	100.0%	77.1 / NA	22	F491L (TTT→TTG)	mltF	membrane‑bound lytic transglycosylase F, murein hydrolase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (10/12);  total (10/12)

AAAATATTCGTAAGTATCAGATTAGCCTGGTGGGTTATCTGCAAGAGAAAGAGAAGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTT  >  NC_000913/2697125‑2697394                                                                                                                                                     |                                                                                                                          aaaaTATTCGTAAGTATCAGATTAGCCTGGTGGGTTATCTGCAAGAGAAAGAGAAGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGttgct                                                                                                                         <  2:271148/151‑1 (MQ=255)    atatTCGTAAGTATCAGATTAGCCTGGTGGGTTATCTGCAAGAGAAAGAGAAGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGttgcttt                                                                                                                       >  2:277924/1‑150 (MQ=255)              gTATCAGATTAGCCTGGTGGGTTATCTGCAAGAGAAAGAGAAGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTGCTTTCCCAGTCGtca                                                                                                            >  1:89940/1‑151 (MQ=255)                aTCAGATTAGCCTGGTGGGTTATCTGCAAGAGAAAGAGAAGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTGCTTTCCCAGTCGtcatc                                                                                                          <  2:78906/151‑1 (MQ=255)                    gATTAGCCTGGTGGGTTATCTGCAAGAGAAAGAGAAGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTGCTTTCCCAGTCGTCATCaaa                                                                                                       >  1:123168/1‑150 (MQ=255)                    gATTAGCCTGGTGGGTTATCTGCAAGAGAAAGAGAAGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTGCTTTCCCAGTCGTCATCaaa                                                                                                       >  2:248022/1‑150 (MQ=255)                     aTTAGCCTGGTGGGTTATCTGCAAGAGAAAGAGAAGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTGCTTTCCCAGTCGTCATCAAAc                                                                                                      <  1:33269/150‑1 (MQ=255)                     aTTAGCCTGGTGGGTTATCTGCAAGAGAAAGAGAAGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTGCTTTCCCAGTCGTCATCAAAc                                                                                                      <  2:170684/150‑1 (MQ=255)                                     aTCTGCAAGAGAAAGAGAAGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTGCTTTCCCAGTCGTCATCAAACTATTTGACCCATtctc                                                                                      >  2:54600/1‑150 (MQ=255)                                        tGCAAGAGAAAGAGAAGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTGCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCctc                                                                                  <  1:24769/151‑1 (MQ=255)                                             gagaaagagaaGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTGCTTTCCCAGTCGTCATCAAACt                                                                                                     <  1:41404/127‑1 (MQ=255)                                             gagaaagagaaGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTGCTTTCCCAGTCGTCATCAAACt                                                                                                     >  2:41404/1‑127 (MQ=255)                                                         cAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTGCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAgg                                                                  <  1:232785/150‑1 (MQ=255)                                                           ggCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTGCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGaaa                                                               >  2:91902/1‑151 (MQ=255)                                                            gCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTGCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGa                                                                 <  2:4062/148‑1 (MQ=255)                                                             cTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTGCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAgg                                                             >  2:242069/1‑151 (MQ=255)                                                             cTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTGCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAgg                                                             <  2:186463/151‑1 (MQ=255)                                                                                aCTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTGCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAAt                                          <  2:196893/151‑1 (MQ=255)                                                                                   ggCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTGCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTaa                                       >  1:26531/1‑151 (MQ=255)                                                                                     cgcAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTGCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATc                                     <  1:196654/151‑1 (MQ=255)                                                                                                     ggTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTGCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTgcg                       >  2:25960/1‑149 (MQ=255)                                                                                                            ccTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTGCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTc               <  1:46785/150‑1 (MQ=255)                                                                                                                    gTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTGCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTa      >  1:6105/1‑151 (MQ=255)                                                                                                                         gCAAAGAGAAATTTCCTTTTCTCTCGTTGCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAAttt  <  1:35032/150‑1 (MQ=255)                                                                                                                                                     |                                                                                                                          AAAATATTCGTAAGTATCAGATTAGCCTGGTGGGTTATCTGCAAGAGAAAGAGAAGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTT  >  NC_000913/2697125‑2697394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑