Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,740,932	A→C	Y73* (TAT→TAG)	aroF ←	3‑deoxy‑D‑arabino‑heptulosonate‑7‑phosphate synthase, tyrosine‑repressible

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,740,932	0	A	C	100.0%	101.5 / NA	30	Y73* (TAT→TAG)	aroF	3‑deoxy‑D‑arabino‑heptulosonate‑7‑phosphate synthase, tyrosine‑repressible
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (14/16);  total (14/16)

CATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGTGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATCGACGAGCATATTCCAGAGCAGTTTCCGGATCATGAATGGAACAAGGACCACATACTACCAGCAGACGAGGATCGCGCCCGGCGATAATATCTGAAATGCTTTTACGCGAGTCAGCAATCTGGGCTTCTTGTTGCAGGCTCAATGGAAAAG  >  NC_000913/2740793‑2741074                                                                                                                                            |                                                                                                                                               cATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGTGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATCGACGAGCCTATTCCagag                                                                                                                                      >  2:9240/1‑150 (MQ=255) cATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGTGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATCGACGAGCCTATTCCagag                                                                                                                                      >  2:251214/1‑150 (MQ=255)   tCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGTGGTACGGGGTCTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATCGACGAGCCTATTCCAGAGCAg                                                                                                                                   >  1:108063/1‑151 (MQ=255)            ccatccatATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGTGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATCGACGAGCCTATTCCAGAGCAGTTTCCGGa                                                                                                                           <  1:315892/150‑1 (MQ=255)               tccatATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGTGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATCGACGAGCCTATTCCAGAGCAGTTTCCGGATCAt                                                                                                                       >  2:207945/1‑151 (MQ=255)                ctctttttggctggTTAATTAATATTTTCCAGCCGACGGTGGGACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAAGCTATCGCTGACCTCTGCGGCAAGGGCTTTAATTCGCCGCGCCTATTCCAGAGCAGTTTCCGGACCATg                                                                                                                      >  2:270870/14‑151 (MQ=255)                 catATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGTGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATCGACGAGCCTATTCCAGAGCAGTTTCCGGATCATGa                                                                                                                     >  1:224687/1‑151 (MQ=255)                   tatGGGGATCGTTAATTAACCCTTTCCAGCCGACAGTGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATCGACGAGCCTATTCCAGAGCAGTTTCCGGATCATGAAt                                                                                                                   <  1:46199/151‑1 (MQ=255)                             gTTAATTAACCCTTTCCAGCCGACAGTGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATCGACGAGCCTATTCCAGAGCAGTTTCCGGATCATGAATGGAACAAGGa                                                                                                         >  1:276392/1‑151 (MQ=255)                                     aCCCTTTCCAGCCGACAGTGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATCGACGAGCCTATTCCAGAGCAGTTTCCGGATCATGAATGGAACAAGGACCACAtac                                                                                                 <  1:300714/151‑1 (MQ=255)                                         tttCCAGCCGACAGTGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATCGACGAGCCTATTCCAGAGCAGTTTCCGGATCATGAATGGAACAAGGACCACATACTAcc                                                                                             <  2:40904/151‑1 (MQ=255)                                         tttCCAGCCGACAGTGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATCGACGAGCCTATTCCAGAGCAGTTTCCGGATCATGAATGGAACAAGGACCACATACTAcc                                                                                             <  2:129649/151‑1 (MQ=255)                                                      gTGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATCGACGAGCCTATTCCAGAGCAGTTTCCGGATCATGAATGGAACAAGGACCACATACTACCAGCAGACGAGGAt                                                                                >  1:196877/1‑151 (MQ=255)                                                       tGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATCGACGAGCCTATTCCAGAGCAGTTTCCGGATCATGAATGGAACAAGGACCACATACTACCAGCAGACGAGGAt                                                                                <  1:167732/150‑1 (MQ=255)                                                       tGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATCGACGAGCCTATTCCAGAGCAGTTTCCGGATCATGAATGGAACAAGGACCACATACTACCAGCAGACGAGGAt                                                                                <  1:27627/150‑1 (MQ=255)                                                       tGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATCGACGAGCCTATTCCAGAGCAGTTTCCGGATCATGAATGGAACAAGGACCACATACTACCAGCAGACGAGGAt                                                                                <  1:188443/150‑1 (MQ=255)                                                       tGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATCGACGAGCCTATTCCAGAGCAGTTTCCGGATCATGAATGGAACAAGGACCACATACTACCAGCAGACGAGGAt                                                                                <  1:67489/150‑1 (MQ=255)                                                              gggTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATCGACGAGCCTATTCCAGAGCAGTTTCCGGATCATGAATGGAACAAGGACCACATACTACCAGCAGACGAGGATCGCGCCCg                                                                        >  1:53718/1‑151 (MQ=255)                                                                   tttCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATCGACGAGCCTATTCCAGAGCAGTTTCCGGATCATGAATGGAACAAGGACCACATACTACCAGCAGACGAGGATCGCGCCCGGCGat                                                                   >  1:68101/1‑151 (MQ=255)                                                                        aaaTAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATCGACGAGCCTATTCCAGAGCAGTTTCCGGATCATGAATGGAACAAGGACCACATACTACCAGCAGACGAGGATCGCGCCCGGCGATAatat                                                              >  2:30113/1‑151 (MQ=255)                                                                               cgcgCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATCGACGAGCCTATTCCAGAGCAGTTTCCGGATCATGAATGGAACAAGGACCACATACTACCAGCAGACGAGGATCGCGCCCGGCGATAATATCTGAAAt                                                       <  1:155529/151‑1 (MQ=255)                                                                                       aCCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATCGACGAGCCTATTCCAGAGCAGTTTCCGGATCATGAATGGAACAAGGACCACATACTACCAGCAGACGAGGATCGCGCCCGGCGATAATATCTGAAATGCTTTTa                                                >  2:201685/1‑150 (MQ=255)                                                                                        ccAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATCGACGAGCCTATTCCAGAGCAGTTTCCGGATCATGAATGGAACAAGGACCACATACTACCAGCAGACGAGGATCGCGCCCGGCGATAATATCTGAAATGCTTTTAcg                                              <  1:40225/151‑1 (MQ=255)                                                                                            aTAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATCGACGAGCCTATTCCAGAGCAGTTTCCGGATCATGAATGGAACAAGGACCACATACTACCAGCAGACGAGGATCGCGCCCGGCGATAATATCTGAAATGCTTTTAcgc                                             <  1:267892/148‑1 (MQ=255)                                                                                                   cTATCGCTGACCTCTGCGGCAAGGGCTTTAAATCGACGAGCCTATTCCAGAGCAGTTTCCGGATCATGAATGGAACAAGGACCACATACTACCAGCAGACGAGGATCGCGCCCGGCGATAATATCTGAAATGCTTTTACGCGAGTCAGCaa                                   <  2:190674/151‑1 (MQ=255)                                                                                                      tCGCTGACCTCTGCGGCAAGGGCTTTAAATCGACGAGCCTATTCCAGAGCAGTTTCCGGATCATGAATGGAACAAGGACCACATACTACCAGCAGACGAGGATCGCGCCCGGCGATAATATCTGAAATGCTTTTACGCGAGTCAGCAAt                                  >  1:248362/1‑149 (MQ=255)                                                                                                             ccTCTGCGGCAAGGGCTTTAAATCGACGAGCCTATTCCAGAGCAGTTTCCGGATCATGAATGGAACAAGGACCACATACTACCAGCAGACGAGGATCGCGCCCGGCGATAATATCTGAAATGCTTTTACGCGAGTCAGCAATCTGGGctt                          >  1:145346/1‑150 (MQ=255)                                                                                                                                aaaTCGACGAGCCTATTCCAGAGCAGTTTCCGGATCATGAATGGAACAAGGACCACATACTACCAGCAGACGAGGATCGCGCCCGGCGATAATATCTGAAATGCTTTTACGCGAGTCAGCAATCTGGGCTTCTTGTTGCAGGCTCAATGGa      <  2:276390/151‑1 (MQ=255)                                                                                                                                    cgacgaGCCTATTCCAGAGCAGTTTCCGGATCATGAATGGAACAAGGACCACATACTACCAGCAGACGAGGATCGCGCCCGGCGATAGTATCTGAAATGCTTTTACGCGAGTCAGCAATCTGGGCTTCTTGTTGCAGGCTCAATGGAAAAg  <  1:111485/151‑1 (MQ=255)                                                                                                                                    cgacgaGCCTATTCCAGAGCAGTTTCCGGATCATGAATGGAACAAGGACCACATACTACCAGCAGACGAGGATCGCGCCCGGCGATAATATCTGAAATGCTTTTACGCGAGTCAGCAATCTGGGCTTCTTGTTGCAGGCTCAATGGAAAAg  <  2:99157/151‑1 (MQ=255)                                                                                                                                            |                                                                                                                                               CATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGTGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATCGACGAGCATATTCCAGAGCAGTTTCCGGATCATGAATGGAACAAGGACCACATACTACCAGCAGACGAGGATCGCGCCCGGCGATAATATCTGAAATGCTTTTACGCGAGTCAGCAATCTGGGCTTCTTGTTGCAGGCTCAATGGAAAAG  >  NC_000913/2740793‑2741074

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑