Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,807,400	A→C	H90P (CAT→CCT)	proX →	glycine betaine/proline ABC transporter periplasmic binding protein; cold shock protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,807,400	0	A	C	100.0%	88.9 / NA	25	H90P (CAT→CCT)	proX	glycine betaine/proline ABC transporter periplasmic binding protein; cold shock protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (11/14);  total (11/14)

GTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCGCTTGCTTCCGGCGATGCAACCTTCACCGCCGTGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGACCAGTACAAAATCACCAACATCGCACAACTGAAAGAT  >  NC_000913/2807277‑2807542                                                                                                                            |                                                                                                                                               gTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCGCTTGCTTCCGGCGATGCAACCTTCACCGCCGTGAACTGGACGCCACTGCCTGACAACATGTACGAAGCTGCCGGTgg                                                                                                                     >  2:211437/1‑151 (MQ=255) gTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCGCTTGCTTCCGGCGATGCAACCTTCACCGCCGTGAACTGGACGCCACTGCCTGACAACATGTACGAAGCTGCCGGTgg                                                                                                                     >  2:205675/1‑151 (MQ=255) gTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCGCTTGCTTCCGGCGATGCAACCTTCACCGCCGTGAACTGGACGCCACTGCCTGACAACATGTACGAAGCTGCCGGTgg                                                                                                                     >  1:44347/1‑151 (MQ=255)         cTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCGCTTGCTTCCGGCGATGCAACCTTCACCGCCGTGAACTGGACGCCACTGCCTGACAACATGTACGAAGCTGCCGGTGGCGATAAGa                                                                                                             >  1:92764/1‑151 (MQ=255)             agaAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCGCTTGCTTCCGGCGATGCAACCTTCACCGCCGTGAACTGGACGCCACTGCCTGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAAt                                                                                                          <  1:178207/150‑1 (MQ=255)                      gTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCGCTTGCTTCCGGCGATGCAACCTTCACCGCCGTGAACTGGACGCCACTGCCTGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGa                                                                                                >  1:184905/1‑151 (MQ=255)                           cccGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCGCTTGCTTCCGGCGATGCACCCTTCACCGCCGTGAACTGGACGCCACTGCCTGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAgggg                                                                                           <  1:241904/150‑1 (MQ=255)                                 aacaacCCCAGCGAAGTAGATTACAACGTTGGCTACCCCTCGCTTGCTTCCGGCGATGCACCCTTCACCGCCGTGAACTGGACGCCACTGCCTGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTg                                                                                     <  2:169933/151‑1 (MQ=255)                                 aacaaACCCAGCGAAGTAGATTACAACGTTGGCTACCCCTCGCTTGCTTCCGGCGATGCAACCTTCACCGCCGTGAACTGGACGCCACTGCCTGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTg                                                                                     <  1:285382/151‑1 (MQ=255)                                  acaaACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCGCTTGCTTCCGGCGATGCAACCTTCACCGCCGTGAACTGGACGCCACTGCCTGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGt                                                                                    <  1:290052/151‑1 (MQ=255)                                      ccccAGCGAAGTAGATTACAACGTTGGCTACACCTCGCTTGCTTCCGGCGATGCAACCTTCACCGCCGTGAACTGGACGCCACTGCCTGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAAc                                                                                <  1:31725/150‑1 (MQ=255)                                      aCCCAGCGAAGTAGATTACAACGTTGGCTACACCTCGCTTGCTTCCGGCGATGCAACCTTCACCGCCGTGAACTGGACGCCACTGCCTGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTaa                                                                                 >  1:65044/1‑150 (MQ=255)                                                 tAGATTACACCGTTGGCTACCCCCCGCTTGCTTCCGGCGATGAACCCTTCACCGCCGTGAACTGGACGCCACTGCCTGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGcac                                                                      <  2:206357/150‑1 (MQ=255)                                                  aGATTACAACGTTGGCTACACCTCGCTTGCTTCCGGCGATGCAACCTTCACCGCCGTGAACTGGACGCCACTGCCTGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAg                                                                    >  2:79977/1‑151 (MQ=255)                                                     ttACAACGTTGTATCCTCCTCGCTTGCTGCGTGGATTGCAACCTTCGCCGCCGTGAACTGGACGCCACTGCCTGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGt                                                                 <  1:242529/151‑1 (MQ=255)                                                         aaCGTGGGCTACCCCTCGCTTGCTTCCGGCGATGCACCCTTCACCGCCGTGAACTGGACGCCACTGCCTGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTAcc                                                             <  2:224120/151‑1 (MQ=255)                                                          ccGTTGGCTACCCCTCGCTTGCTCCCGGCGATGCACCCTTCACCGCCGTGAACTGGACGCCACTGCCTGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCt                                                            <  2:90879/150‑1 (MQ=255)                                                            gTTGGCTACACCTCGCTTGCTTCCGGCGATGCAAACTTCACCGCCGTGAACTGGACGCCACTGCCTGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGa                                                          >  2:180294/1‑151 (MQ=255)                                                             ttGGCTCCCCCTCGCTTCTTTCCGGCGAGGCAACCTTCACCGCCGTGAACTGGACGCCACTGCCTGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGa                                                          <  2:187182/150‑1 (MQ=255)                                                                gCTACACCTCGCTTGCTTCCGGCGATGCAACCTTCACCGCCGTGAACTGGACGCCACTGCCTGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGa                                                      >  2:107351/1‑151 (MQ=255)                                                                      ccTCGCTTGCTTCCGGCGATGCAACCTTCACCGCCGTGAACTGGACGCCACTGCCTGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGaa                                                <  1:205678/151‑1 (MQ=255)                                                                                          gCAACCTTCACCGCCGTGAACTGGACGCCACTGCCTGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGACCAGTACaaaa                            >  2:317233/1‑151 (MQ=255)                                                                                                    ccgccgTGAACTGGACGCCACTGCCTGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGACCAGTACAAAATCACCAACAt                  <  2:184904/151‑1 (MQ=255)                                                                                                                   cGCCACTGCCTGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGACCAGTACAAAATCACCAACATCGCACAACTGAAAGa   <  1:71256/151‑1 (MQ=255)                                                                                                                     ccACTGCCTGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGACCAGTACAAAATCACCAACATCGCACAACTGAAAGAt  >  1:44001/1‑150 (MQ=255)                                                                                                                            |                                                                                                                                               GTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCGCTTGCTTCCGGCGATGCAACCTTCACCGCCGTGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGACCAGTACAAAATCACCAACATCGCACAACTGAAAGAT  >  NC_000913/2807277‑2807542

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑