Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,858,593	T→G	Y501D (TAC→GAC)	mutS →	methyl‑directed mismatch repair protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,858,593	0	T	G	100.0%	62.2 / NA	19	Y501D (TAC→GAC)	mutS	methyl‑directed mismatch repair protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (7/12);  total (7/12)

CGTGAACGTACCGGCCTGGACACGCTGAAAGTTGGCTTTAATGCGGTGCACGGCTACTACATTCAAATCAGCCGTGGGCAAAGCCATCTGGCACCCATCAACTACATGCGTCGCCAGACGCTGAAAAACGCCGAGCGCTACATCATTCCAGAGCTAAAAGAGTACGAAGATAAAGTTCTCACCTCAAAAGGCAAAGCACTGGCACTGGAAAAACAGCTTTATGAAGAGCTGTTCGACCTGCTGTTGCCGCATCTGGAAGCGTTGCAACAGAGCGCGAGCG  >  NC_000913/2858455‑2858734                                                                                                                                           |                                                                                                                                              cGTGAACGTACCGGCCTGGACACGCTGAAAGTTGGCTTTAATGCGGTGCACGGCTACTACATTCAAATCAGCCGTGGGCAAAGCCATCTGGCACCCATCAACTACATGCGTCGCCAGACGCTGAAAAACGCCGAGCGCGACATCATTcc                                                                                                                                     <  2:260098/149‑1 (MQ=255)          aCCGGCCTGGACACGCTGAAAGTTGGCTTTAATGCGGTGCACGGCTACTACATTCAAATCAGCCGTGGGCAAAGCCATCTGGCACCCATCAACTACATGCGTCGCCAGACGCTGAAAAACGCCGAGCGCGACATCATTCCAGAGCTAAAag                                                                                                                          <  2:287032/151‑1 (MQ=255)          aCCGGCCTGGACACGCTGAAAGTTGGCTTTAATGCGGTGCACGGCTACTACATTCAAATCAGCCGTGGGCAAAGCCATCTGGCACCCATCAACTACATGCGTCGCCAGACGCTGAAAAACGCCGAGCGCGACATCATTCCAGAGCTAAAag                                                                                                                          <  2:121913/151‑1 (MQ=255)            cGGCCTGGACACGCTGAAAGTTGGCTTTAATGCGGTGCACGGCTACTACATTCAAATCAGCCGTGGGCAAAGCCATCTGGCACCCATCAACTACATGCGTCGCCAGACGCTGAAAAACGCCGAGCGCGACATCATTCCAGAGCTAAAagag                                                                                                                        <  2:300344/151‑1 (MQ=255)             ggCCTGGACACGCTGAAAGTTGGCTTTAATGCGGTGCACGGCTACTACATTCAAATCAGCCGTGGGCAAAGCCATCTGGCACCCATCAACTACATGCGTCGCCAGACGCTGAAAAACGCCGAGCGCGACATCATTCCAGAGCTAAAAGAGt                                                                                                                       >  1:58619/1‑151 (MQ=255)                           gAAAGTTGGCTTTAATGCGGTGCACGGCTACTACATTCAAATCAGCCGTGGGCAAAGCCATCTGGCACCCATCAACTACATGCGTCGCCAGACGCTGAAAAACGCCGAGCGCGACATCATTCCAGAGCTAAAAGAGTACGAAGATAAAGtt                                                                                                         >  1:69621/1‑151 (MQ=255)                                  ggCTTTAATGCGGTGCACGGCTACTACATTCAAATCAGCCGTGGGCAAAGCCATCTGGCACCCATCAACTACATGCGTCGCCAGACGCTGAAAAACGCCGAGCGCGACATCATTCCAGAGCTAAAAGAGTACGAAGATAAAGTTCTCAc                                                                                                    >  1:223744/1‑149 (MQ=255)                                        aaTGCGGTGCACGGCTACTACATTCAAATCAGCCGTGGGCAAAGCCATCTGGCACCCATCAACTACATGCGTCGCCAGACGCTGAAAAACGCCGAGCGCGACATCATTCCAGAGCTAAAAGAGTACGAAGATAAAGTTCTCACCTCAAAAg                                                                                            <  1:235158/151‑1 (MQ=255)                                        aaTGCGGTGCACGGCTACTACATTCAAATCAGCCGTGGGCAAAGCCATCTGGCACCCATCAACTACATGCGTCGCCAGACGCTGAAAAACGCCGAGCGCGACATCATTCCAGAGCTAAAAGAGTACGAAGATAAAGTTCTCACCTCAAAAg                                                                                            <  1:258343/151‑1 (MQ=255)                                                 cACGGCTACTACATTCAAATCAGCCGTGGGCAAAGCCATCTGGCACCCATCAACTACATGCGTCGCCAGACGCTGAAAAACGCCGAGCGCGACATCATTCCAGAGCTAAAAGAGTACGAAGATAAAGTTCTCACCTCAAAAGGCAAAgcac                                                                                   <  1:231075/151‑1 (MQ=255)                                                      ctactaCATTCAAATCAGCCGTGGGCAAAGCCATCTGGCACCCATCAACTACATGCGTCGCCAGACGCTGAAAAACGCCGAGCGCGACATCATTCCAGAGCTAAAAGAGTACGAAGATAAAGTTCTCACCTCAAAAGGCAAAgcactggca                                                                              >  2:36929/1‑151 (MQ=255)                                                                           tGGGCAAAGCCATCTGGCACCCATCAACTACATGCGTCGCCAGACGCTGAAAAACGCCGAGCGCGACATCATTCCAGAGCTAAAAGAGTACGAAGATAAAGTTCTCACCTCAAAAGGCAAAGCACTGGCACTGGAAAAACAGCTTTATGa                                                          <  1:143854/150‑1 (MQ=255)                                                                               cAAAGCCATCTGGCACCCATCAACTACATGCGTCGCCAGACGCTGAAAAACGCCGAGCGCGACATCATTCCAGAGCTAAAAGAGTACGAAGATAAAGTTCTCACCTCAAAAGGCAAAGCACTGGCACTGGAAAAACAGCTTTATGAAGAGc                                                     <  1:250087/151‑1 (MQ=255)                                                                                                cATCAACTACATGCGTCGCCAGACGCTGAAAAACGCCGAGCGCGACATCATTCCAGAGCTAAAAGAGTACGAAGATAAAGTTCTCACCTCAAAAGGCAAAGCACTGGCACTGGAAAAACAGCTTTATGAAGAGCTGTTCGACCTGCTGTTg                                    >  2:141841/1‑151 (MQ=255)                                                                                                             cGTCGCCAGACGCTGAAAAACGCCGAGCGCGACATCATTCCAGAGCTAAAAGAGTACGAAGATAAAGTTCTCACCTCAAAAGGCAAAGCACTGGCACTGGAAAAACAGCTTTATGAAGAGCTGTTCGACCTGCTGTTGCCGCATCTGGaa                        <  2:210173/150‑1 (MQ=255)                                                                                                             cGTCGCCAGACGCTGAAAAACGCCGAGCGCGACATCATTCCAGAGCTAAAAGAGTACGAAGATAAAGTTCTCACCTCAAAAGGCAAAGCACTGGCACTGGAAAAACAGCTTTATGAAGAGCTGTTCGACCTGCTGTTGCCGCATCTGGAAg                       <  2:47107/151‑1 (MQ=255)                                                                                                                 gCCAGACGCTGAAAAACGCCGAGCGCGACATCATTCCAGAGCTAAAAGAGTACGAAGATAAAGTTCTCACCTCAAAAGGCAAAGCACTGGCACTGGAAAAACAGCTTTATGAAGAGCTGTTCGACCTGCTGTTGCCGCATCTGGAAGCGtt                   >  1:285103/1‑151 (MQ=255)                                                                                                                           gAAAAACGCCGAGCGCGACATCATTCCAGAGCTAAAAGAGTACGAAGATAAAGTTCTCACCTCAAAAGGCAAAGCACTGGCACTGGAAAAACAGCTTTATGAAGAGCTGTTCGACCTGCTGTTGCCGCATCTGGAAGCGTTGCAACagag          >  2:207855/1‑150 (MQ=255)                                                                                                                                   ccGAGCGCGACATCATTCCAGAGCTAAAAGAGTACGAAGATAAAGTTCTCACCTCAAAAGGCAAAGCACTGGCACTGGAAAAACAGCTTTATGAAGAGCTGTTCGACCTGCTGTTGCCGCATCTGGAAGCGTTGCAACAgagcgcgagcg  <  1:305224/150‑1 (MQ=255)                                                                                                                                           |                                                                                                                                              CGTGAACGTACCGGCCTGGACACGCTGAAAGTTGGCTTTAATGCGGTGCACGGCTACTACATTCAAATCAGCCGTGGGCAAAGCCATCTGGCACCCATCAACTACATGCGTCGCCAGACGCTGAAAAACGCCGAGCGCTACATCATTCCAGAGCTAAAAGAGTACGAAGATAAAGTTCTCACCTCAAAAGGCAAAGCACTGGCACTGGAAAAACAGCTTTATGAAGAGCTGTTCGACCTGCTGTTGCCGCATCTGGAAGCGTTGCAACAGAGCGCGAGCG  >  NC_000913/2858455‑2858734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑