Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,910,354	A→G	V111A (GTG→GCG)	mazG ←	nucleoside triphosphate pyrophosphohydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,910,354	0	A	G	100.0%	62.0 / NA	19	V111A (GTG→GCG)	mazG	nucleoside triphosphate pyrophosphohydrolase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (7/12);  total (7/12)

CAATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTAAAGCCGGTAAACTACGAGGAATATCGTCCAGCGCCGAATGCTGCGCTTTCTGCGCGCGCTCTTCGGTTTTGATTTGCTCCCAACGGGCAAGCACTTCACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAATAGCAGCGCAAATATCATTAAAGTCAAAGCGCCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAAT  >  NC_000913/2910210‑2910497                                                                                                                                                 |                                                                                                                                                cAATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTAAAGCCGGTAAACTACGAGGAATATCGTCCAGCGCCGAATGCTGCGCTTTCTGCGCGCGCTCTTCGGTTTTGATTTGCTCCCAACGGGCAAGCGCTTCac                                                                                                                                           <  1:41120/151‑1 (MQ=255)    tCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTAAAGCCGGTAAACTACGAGGAATATCGTCCAGCGCCGAATGCTGCGCTTTCTGCGCGCGCTCTTCGGTTTTGATTTGCTCCCAACGGGCAAGCGCTTCactac                                                                                                                                        <  1:202235/151‑1 (MQ=255)       aaGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTAAAGCCGGTAAACTACGAGGAATATCGTCCAGCGCCGAATGCTGCGCTTTCTGCGCGCGCTCTTCGGTTTTGATTTGCTCCCAACGGGCAAGCGCTTCACTACTg                                                                                                                                      <  2:312035/150‑1 (MQ=255)                                                  ttAAAGCCGGTAAACTACGAGGAATATCGTCCAGCGCCGAATGCTGCGCTTTCTGCGCGCGCTCTTCGGTTTTGATTTGCTCCCAACGGGCAAGCGCTTCACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGctc                                                                                          <  2:142194/151‑1 (MQ=255)                                                        ccGGTAAACTACGAGGAATATCGTCCAGCGCCGAATGCTGCGCTTTCTGCGCGCGCTCTTCGGTTTTGATTTGCTCCCAACGGGCAAGCGCTTCACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAAttt                                                                                    >  1:243216/1‑151 (MQ=255)                                                        ccGGTAAACTACGAGGAATATCGTCCAGCGCCGAATGCTGCGCTTTCTGCGCGCGCTCTTCGGTTTTGATTTGCTCCCAACGGGCAAGCGCTTCACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAAttt                                                                                    >  2:232789/1‑151 (MQ=255)                                                                     aGGAATATCGTCCAGCGCCGAATGCTGCGCTTTCTGCGCGCGCTCTTCGGTTTTGATTTGCTCCCAACGGGCAAGCGCTTCACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAATagc                                                                        <  2:83266/150‑1 (MQ=255)                                                                     aGGAATATCGTCCAGCGCCGAATGCTGCGCTTTCTGCGCGCGCTCTTCGGTTTTGATTTGCTCCCAACGGGCAAGCGCTTCACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAATagc                                                                        <  2:44404/150‑1 (MQ=255)                                                                       gAATATCGTCCAGCGCCGAATGCTGCGCTTTCTGCGCGCGCTCTTCGGTTTTGATTTGCTCCCAACGGGCAAGCGCTTCACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAATagcag                                                                      >  2:317369/1‑150 (MQ=255)                                                                                          aTGCTGCGCTTTCTGCGCGCGCTCTTCGGTTTTGATTTGCTCCCAACGGGCAAGCGCTTCACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAATAGCAGCGCAAATATCATTAAAGTCa                                                  >  1:182510/1‑151 (MQ=255)                                                                                                               ctctTCGGTTTTGATTTGCTCCCAACGGGCAAGCGCTTCACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAATAGCAGCGCAAATATCATTAAAGTCAAAGCGCCCTTCTTCCTGAGc                              <  1:62287/150‑1 (MQ=255)                                                                                                               ctctTCGGTTTTGATTTGCTCCCAACGGGCAAGCGCTTCACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAATAGCAGCGCAAATATCATTAAAGTCAAAGCGCCCTTCTTCCTGAGc                              >  1:1484/1‑150 (MQ=255)                                                                                                                    cGGTTTTGATTTGCTCCCAACGGGCAAGCGCTTCACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAATAGCAGCGCAAATATCATTAAAGTCAAAGCGCCCTTCTTCCTGAGCCATTTg                        <  1:15357/151‑1 (MQ=255)                                                                                                                                 cTCCCAACGGGCAAGCGCTTTACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAATAGCAGCGCAAATATCATTAAAGTCAAAGCGCCCTTCTTCCTGAGCCATTTGCGCGTAAAacac            <  2:230478/150‑1 (MQ=255)                                                                                                                                       aCGGGCAAGCGCTTCACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAATAGCAGCGCAAATATCATTAAAGTCAAAGCGCCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTgg     >  2:151080/1‑151 (MQ=255)                                                                                                                                        cGGGCAAGCGCTTCACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAATAGCAGCGCAAATATCATTAAAGTCAAAGCGCCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGa    <  1:36993/151‑1 (MQ=255)                                                                                                                                        cGGGCAAGCGCTTCACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAATAGCAGCGCAAATATCATTAAAGTCAAAGCGCCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGa    <  1:110150/151‑1 (MQ=255)                                                                                                                                          ggCAAGCGCTTCACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAATAGCAGCGCAAATATCATTAAAGTCAAAGCGCCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAAt  >  1:184779/1‑151 (MQ=255)                                                                                                                                           gCAAGCGCTTCACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAATAGCAGCGCAAATATCATTAAAGTCAAAGCGCCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAAt  <  2:106653/150‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                CAATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTAAAGCCGGTAAACTACGAGGAATATCGTCCAGCGCCGAATGCTGCGCTTTCTGCGCGCGCTCTTCGGTTTTGATTTGCTCCCAACGGGCAAGCACTTCACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAATAGCAGCGCAAATATCATTAAAGTCAAAGCGCCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAAT  >  NC_000913/2910210‑2910497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑