Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,919,456	T→G	I431L (ATC→CTC)	gudX ←	glucarate dehydratase‑related protein, substrate unknown

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,919,456	0	T	G	100.0%	47.7 / NA	15	I431L (ATC→CTC)	gudX	glucarate dehydratase‑related protein, substrate unknown
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (7/8);  total (7/8)

TCAGCATACTGTCATGACCCGCCACCGGGATAACCTGCATTTCAGTAACAACAGGCGTCGTAAATTGAGAACTCATATTTATGTCCTTTTTCAGAATCAATGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCA  >  NC_000913/2919310‑2919580                                                                                                                                                   |                                                                                                                             tcaGCATACTGTCATGACCCGCCACCGGGATAACCTGCATTTCAGTAACAACAGGCGTCGTAAATTGAGAACTCATATTTATGTCCTTTTTCAGAATCAATGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGAGCAgg                                                                                                                          >  1:28886/1‑151 (MQ=255)         cTGTCATGACCCGCCACCGGGATAACCTGCATTTCAGTAACAACAGGCGTCGTAAATTGAGAACTCATATTTATGTCCTTTTTCAGAATCAATGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGAGCAGGTACTGCAt                                                                                                                  >  2:147303/1‑151 (MQ=255)           gTCATGACCCGCCACCGGGATAACCTGCATTTCAGTAACAACAGGCGTCGTAAATTGAGAACTCATATTTATGTCCTTTTTCAGAATCAATGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGAGCAGGTACTGCATCg                                                                                                                >  2:298443/1‑151 (MQ=255)               tGACCCGCCACCGGGATAACCTGCATTTCAGTAACAACAGGCGTCGTAAATTGAGAACTCATATTTATGTCCTTTTTCAGAATCAATGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGAGCAGGTACTGCATCGGAcc                                                                                                            <  2:290790/151‑1 (MQ=255)                         ccGGGATAACCTGCATTTCAGTAACAACAGGCGTCGTAAATTGAGAACTCATATTTATGTCCTTTTTCAGAATCAATGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGAGCAGGTACTGCATCGGACCTGCGTCGTTa                                                                                                  <  1:311402/151‑1 (MQ=255)                                   cTGCATTTCAGTAACAACAGGCGTCGTAAATTGAGAACTCATATTTATGTCCTTTTTCAGAATCAATGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGAGCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCg                                                                                         >  1:215422/1‑150 (MQ=255)                                              tAACAACAGGCGTCGTAAATTGAGAACTCATATTTATGTCCTTTTTCAGAATCAATGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGAGCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACg                                                                              <  1:281159/150‑1 (MQ=255)                                                       gCGTCGTAAATTGAGAACTCATATTTATGTCCTTTTTCAGAATCAATGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGAGCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAg                                                                       <  1:171135/148‑1 (MQ=255)                                                                         tCATATTTATGTCCTTTTTCAGAATCAATGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGAGCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTAcc                                                  <  2:215419/151‑1 (MQ=255)                                                                          cATATTTATGTCCTTTTTCAGAATCAATGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGAGCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTAc                                                   >  2:131030/1‑149 (MQ=255)                                                                           atatTTATGTCCTTTTTCAGAATCAATGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGAGCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTACCTg                                                <  1:93350/151‑1 (MQ=255)                                                                              tttATGTCCTTTTTCAGAATCAATGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGAGCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTACCTGtt                                              >  2:43085/1‑150 (MQ=255)                                                                                tATGTCCTTTTTCAGAATCAATGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGAGCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTACCTGTTccc                                           <  1:7895/151‑1 (MQ=255)                                                                                                     tGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGAGCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGAc                      <  1:186851/151‑1 (MQ=255)                                                                                                                         tttACGGTCAAAGGTCCAGCCGGGGAGCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCa  >  2:116964/1‑151 (MQ=255)                                                                                                                                                   |                                                                                                                             TCAGCATACTGTCATGACCCGCCACCGGGATAACCTGCATTTCAGTAACAACAGGCGTCGTAAATTGAGAACTCATATTTATGTCCTTTTTCAGAATCAATGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCA  >  NC_000913/2919310‑2919580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑