Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,951,714	T→C	S250G (AGC→GGC)	recD ←	exonuclease V (RecBCD complex), alpha chain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,951,714	0	T	C	100.0%	33.8 / NA	12	S250G (AGC→GGC)	recD	exonuclease V (RecBCD complex), alpha chain
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/5);  total (7/5)

ATCGGGCAAGGCGTCGATCAGTCTCGACATCATAGGCAGATCGATCATTGACGCTTCATCTACCACCAGCACATCAAGATGCAGCGGGTTACCGGCATGATGACGTAAACGCTGGCTACCCGGCTGCGCGCCCAGCAATCGGTGCAAAGTGCTGGCATCTTCCGGAATGCGTTTCTTTTGTTCATCGGTCAGCGGTAACTGTCGCAAAGCCTTGCCGAGAGATTCGGTTAAGCGCGCGGCAGCTTTACCCGTTGGTGCAGCCA  >  NC_000913/2951598‑2951860                                                                                                                     |                                                                                                                                                   aTCGGGCAAGGCGTCGATCAGTCTCGACATCATAGGCAGATCGATCATTGACGCTTCATCTACCACCAGCACATCAAGATGCAGCGGGTTACCGGCATGATGACGTAAACGCTGGCCACCCGGCTGCGCGCCCAGCAATCGGTGCAAAGTg                                                                                                                  <  2:297431/151‑1 (MQ=255)  tCGGGCAAGGCGTCGATCAGTCTCGACATCATAGGCAGATCGATCATTGACGCTTCATCTACCACCAGCACATCAAGATGCAGCGGGTTACCGGCATGATGACGTAAACGCTGGCCACCCGGCTGCGCGCCCAGCAAGCGGGGCAAAGGGc                                                                                                                 <  1:186802/151‑1 (MQ=255)                             atcatAGGCAGATCGATCATTGACGCTTCATCTACCACCAGCACATCAAGATGCAGCGGGTTACCGGCATGATGACGTAAACGCTGGCCACCCGGCTGCGCGCCCAGCAATCGGTGCAAAGTGCTGGCATCTTCCGGAATGCGTTTCtttt                                                                                      >  1:282853/1‑151 (MQ=255)                             atcatAGGCAGATCGATCATTGACGCTTCATCTACCACCAGCACATCAAGATGCAGCGGGTTACCGGCATGATGACGTAAACGCTGGCCACCCGGCTGCGCGCCCAGCAATCGGTGCAAAGTGCTGGCATCTTCCGGAATGCGTTTCtttt                                                                                      >  1:35834/1‑151 (MQ=255)                                atAGGCAGATCGATCATTGACGCTTCATCTACCACCAGCACATCAAGATGCAGCGGGTTACCGGCATGATGACGTAAACGCTGGCCACCCGGCTGCGCGCCCAGCAATCGGTGCAAAGTGCTGGCATCTTCCGGAATGCGTTTCTTTTGtt                                                                                   >  1:66936/1‑151 (MQ=255)                                            atcATTGACGCTTCATCTACCACCAGCACATCAAGATGCAGCGGGTTACCGGCATGATGACGTAAACGCTGGCCACCCGGCTGCGCGCCCAGCAATCGGTGCAAAGTGCTGGCATCTTCCGGAATGCGTTTCTTTTGTTCATCGGTCAGCg                                                                       >  2:303118/1‑151 (MQ=255)                                             tcATTGACGCTTCATCTACCACCAGCACATCAAGATGCAGCGGGTTACCGGCATGATGACGTAAACGCTGGCCACCCGGCTGCGCGCCCAGCAATCGGTGCAAAGTGCTGGCATCTTCCGGAATGCGTTTCTTTTGTTCATCGGTCAGCgg                                                                      >  1:67809/1‑151 (MQ=255)                                                       ttCATCTACCACCAGCACATCAAGATGCAGCGGGTTACCGGCATGATGACGTAAACGCTGGCCACCCGGCTGCGCGCCCAGCAATCGGTGCAAAGTGCTGGCATCTTCCGGAATGCGTTTCTTTTGTTCATCGGTCAGCGGTAACTGTCGc                                                            <  2:247136/151‑1 (MQ=255)                                                           tCTACCACCAGCACATCAAGATGCAGAGGGGTACCGGCATGGTGACGTACACGCTGGCCACCCGGCAGCGCGCCCAGCAATCGGAGCAAAGTGATGGCATCTTCCGGAATGCGTTTCTTTTGTTAATCGGACAGAGGCAACTGTCGCAAAg                                                        >  2:151075/1‑151 (MQ=255)                                                                        caTCAAGATGCAGCGGGTTACCGGCATGATGACGTAAACGCTGGCCACCCGGCTGCGCGCCCAGCAATCGGTGCAAAGTGCTGGCATCTTCCGGAATGCGTTTCTTTTGTTCATCGGTCAGCGGTAACTGTCGCAAAGCCTTGCCgagaga                                           >  2:249638/1‑151 (MQ=255)                                                                                                cATGATGACGTAAACGCTGGCCACCCGGCTGCGCGCCCAGCAATCGGTGCAAAGTGCTGGCATCTTCCGGAATGCGTTTCTTTTGTTCATCGGTCAGCGGTAACTGTCGCAAAGCCTTGCCGAGAGATTCGGTTAAGCGCGCGGCAGCtt                    <  1:229886/150‑1 (MQ=255)                                                                                                                 tGGCCACCCGGCTGCGCGCCCAGCAATCGGTGCAAAGTGCTGGCATCTTCCGGAATGCGTTTCTTTTGTTCATCGGTCAGCGGTAACTGTCGCAAAGCCTTGCCGAGAGATTCGGTTAAGCGCGCGGCAGCTTTACCCGTTGGTGCAGCCa  <  2:161497/151‑1 (MQ=255)                                                                                                                     |                                                                                                                                                   ATCGGGCAAGGCGTCGATCAGTCTCGACATCATAGGCAGATCGATCATTGACGCTTCATCTACCACCAGCACATCAAGATGCAGCGGGTTACCGGCATGATGACGTAAACGCTGGCTACCCGGCTGCGCGCCCAGCAATCGGTGCAAAGTGCTGGCATCTTCCGGAATGCGTTTCTTTTGTTCATCGGTCAGCGGTAACTGTCGCAAAGCCTTGCCGAGAGATTCGGTTAAGCGCGCGGCAGCTTTACCCGTTGGTGCAGCCA  >  NC_000913/2951598‑2951860

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑