Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,969,229	A→C	intergenic (‑252/‑433)	rppH ← / → mutH	RNA pyrophosphohydrolase/methyl‑directed mismatch repair protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,969,229	0	A	C	100.0%	49.0 / NA	15	intergenic (‑252/‑433)	rppH/mutH	RNA pyrophosphohydrolase/methyl‑directed mismatch repair protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (11/4);  total (11/4)

TATGTGCAGAGTTATAAACAGAGGAAGGGGTGGATAGCCGTTTTTATTCACCTTTTCTGTGGATAGAGTTGTGAAGAACTACGGAATTACTACGGGAAAACCCGGAGCATTCTGAATAAGCCCGATATAGACATTTTAAAATATTCATTATCAGTCAATGCATTACATGTAATTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACGGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCA  >  NC_000913/2969092‑2969364                                                                                                                                          |                                                                                                                                        tATGTGCAGAGTTATAAACAGAGGAAGGGGTGGATAGCCGTTTTTATTCACCTTTTCTGTGGATAGAGTTGTGAAGAACTACGGAATTACTACGGGAAAACCCGGAGCATTCTGAATAAGCCCGATATAGACATTTTCAAATATTCATTAt                                                                                                                            >  2:203474/1‑151 (MQ=255) tATGTGCAGAGTTATAAACAGAGGAAGGGGTGGATAGCCGTTTTTATTCACCTTTTCTGTGGATAGAGTTGTGAAGAACTACGGAATTACTACGGGAAAACCCGGAGCATTCTGAATAAGCCCGATATAGACATTTTCAAATATTCATTAt                                                                                                                            >  2:291180/1‑151 (MQ=255)    gtgCAGAGTTATAAACAGAGGAAGGGGTGGATAGCCGTTTTTATTCACCTTTTCTGTGGATAGAGTTGTGAAGAACTACGGAATTACTACGGGAAAACCCGGAGCATTCTGAATAAGCCCGATATAGACATTTTCAAATATTCATTATCAg                                                                                                                         >  1:182322/1‑151 (MQ=255)         gagTTATAAACAGAGGAAGGGGTGGATAGCCGTTTTTATTCACCTTTTCTGTGGATAGAGTTGTGAAGAACTACGGAATTACTACGGGAAAACCCGGAGCATTCTGAATAAGCCCGATATAGACATTTTCAAATATTCATTATCAGTCAAt                                                                                                                    >  1:229214/1‑151 (MQ=255)           gTTATAAACAGAGGAAGGGGTGGATAGCCGTTTTTATTCACCTTTTCTGTGGATAGAGTTGTGAAGAACTACGGAATTACTACGGGAAAACCCGGAGCATTCTGAATAAGCCCGATATAGACATTTTCAAATATTCATTATCAGTCAATg                                                                                                                   >  1:180620/1‑150 (MQ=255)           gTTATAAACAGAGGAAGGGGTGGATAGCCGTTTTTATTCACCTTTTCTGTGGATAGAGTTGTGAAGAACTACGGAATTACTACGGGAAAACCCGGAGCATTCTGAATAAGCCCGATATAGACATTTTCAAATATTCATTATCAGTCAATg                                                                                                                   >  1:210666/1‑150 (MQ=255)           gTTATAAACAGAGGAAGGGGTGGATAGCCGTTTTTATTCACCTTTTCTGTGGATAGAGTTGTGAAGAACTACGGAATTACTACGGGAAAACCCGGAGCATTCTGAATAAGCCCGATATAGACATTTTCAAATATTCATTATCAGTCAATg                                                                                                                   >  2:301841/1‑150 (MQ=255)                                                                     ttGTGAAGAACTACGGAATTACTACGGGAAAACCCGGAGCATTCTGAATAAGCCCGATATAGACATTTTCAAATATTCATTATCAGTCAATGCATTACATGTAATTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCa                                                        <  1:296140/151‑1 (MQ=255)                                                                     ttGTGAAGAACTACGGAATTACTACGGGAAAACCCGGAGCATTCTGAATAAGCCCGATATAGACATTTTCAAATATTCATTATCAGTCAATGCATTACATGTAATTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCa                                                        <  2:12108/151‑1 (MQ=255)                                                                                    gAATTACTACGGGAAAACCCGGAGCATTCTGAATAAGCCCGATATAGACATTTTCAAATATTCATTATCAGTCAATGCATTACATGTAATTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACGGGTTGGCGa                                         >  2:10328/1‑151 (MQ=255)                                                                                         actacGGGAAAACCCGGAGCATTCTGAATAAGCCCGATATAGACATTTTCAAATATTCATTATCAGTCAATGCATTACATGTAATTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACGGGTTGGCGAAAGAt                                    <  1:203477/151‑1 (MQ=255)                                                                                          ctacGGGAAAACCCGGAGCATTCTGAATAAGCCCGATATAGACATTTTCAAATATTCATTATCAGTCAATGCATTACATGTAATTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACGGGTTGGCGAAAGAt                                    >  2:248419/1‑150 (MQ=255)                                                                                               ggAAAACCCGGAGCATTCTGAATAAGCCCGATATAGACATTTTCAAATATTCATTATCAGTCAATGCATTACATGTAATTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACGGGTTGGCGAAAGATCAAcc                               <  1:291182/150‑1 (MQ=255)                                                                                                                        gCCCGATATAGACATTTTCAAATATTCATTATCAGTCAATGCATTACATGTAATTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACGGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAAtgtg     >  2:28742/1‑151 (MQ=255)                                                                                                                             atatAGACATTTTCAAATATTCATTATCAGTCAATGCATTACATGTAATTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACGGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCa  >  1:205950/1‑149 (MQ=255)                                                                                                                                          |                                                                                                                                        TATGTGCAGAGTTATAAACAGAGGAAGGGGTGGATAGCCGTTTTTATTCACCTTTTCTGTGGATAGAGTTGTGAAGAACTACGGAATTACTACGGGAAAACCCGGAGCATTCTGAATAAGCCCGATATAGACATTTTAAAATATTCATTATCAGTCAATGCATTACATGTAATTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACGGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCA  >  NC_000913/2969092‑2969364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑