Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,028,941	G→A	V24V (GTC→GTT)	ygfS ←	putative 4Fe‑4S ferredoxin‑type oxidoreductase subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,028,941	0	G	A	100.0%	79.5 / NA	22	V24V (GTC→GTT)	ygfS	putative 4Fe‑4S ferredoxin‑type oxidoreductase subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/13);  total (9/13)

TCAGCGCCCCCACGGGGCAAGCGCCAACACAAGGGGCGTTTTCACACTGATGGCACATCACCGGCGCACTGATGCTGTCCAGTCGCTGTACCTTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCGTATCAAACAGAGTTAACATATCGCGCGCCGCCTGCCTTCCTGCGGCCATTGCAGTGACAACC  >  NC_000913/3028796‑3029087                                                                                                                                                  |                                                                                                                                                   tCAGCGCCCCCACGGGGCAAGCGCCAACACAAGGGGCGTTTTCACACTGATGGCACATCACCGGCGCACTGATGCTGTCCAGTCGCTGTACCTTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCAACca                                                                                                                                                <  1:318535/150‑1 (MQ=255)        ccccACGGGGCAAGCGCCAACACAAGGGGCGTTTTCACACTGATGGCACATCACCGGCGCACTGATGCTGTCCAGTCGCTGTACCTTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCAACCACACAGGCt                                                                                                                                        <  2:11645/151‑1 (MQ=255)         cccACGGGGCAAGCGCCAACACAAGGGGCGTTTTCACACTGATGGCACATCACCGGCGCACTGATGCTGTCCAGTCGCTGTACCTTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCAACCACACAGGCTa                                                                                                                                       >  2:251046/1‑151 (MQ=255)                        ccAACACAAGGGGCGTTTTCACACTGATGGCACATCACCGGCGCACTGATGCTGTCCAGTCGCTGTACCTTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCAACCACACAGGCTACTTCACAAGTACGGc                                                                                                                        <  1:87119/151‑1 (MQ=255)                            cacaAGGGGCGTTTTCACACTGATGGCACATCACCGGCGCACTGATGCTGTCCAGTCGCTGTACCTTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCAACCACACAGGCTACTTCACAAGTACGGCAGc                                                                                                                     >  1:312932/1‑150 (MQ=255)                                  gggCGTTTTCACACTGATGGCACATCACCGGCGCACTGATGCTGTCCAGTCGCTGTACCTTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCAACCACACAGGCTACTTCACAAGTACGGCAGCCAATAc                                                                                                               <  1:52704/150‑1 (MQ=255)                                       ttttCACACTGATGGCACATCACCGGCGCACTGATGCTGTCCAGTCGCTGTACCTTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCAACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCa                                                                                                          <  2:209762/150‑1 (MQ=255)                                       ttttCACACTGATGGCACATCACCGGCGCACTGATGCTGTCCAGTCGCTGTACCTTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCAACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAg                                                                                                         >  1:201723/1‑151 (MQ=255)                                                 gATGGCACATCACCGGCGCACTGATGCTGTCCAGTCGCTGTACCTTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCAACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTaa                                                                                                >  1:273161/1‑150 (MQ=255)                                                   tGGCACATCACCGGCGCACTGATGCTGTCCAGTCGCTGTACCTTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCAACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGa                                                                                             <  2:126706/151‑1 (MQ=255)                                                                   cACTGATGCTGTCCAGTCGCTGTACCTTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCAACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTtcat                                                                             >  1:22836/1‑151 (MQ=255)                                                                             gTCCAGTCGCTGTACCTTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCAACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGc                                                                     >  2:252174/1‑149 (MQ=255)                                                                                   tCGCTGTACCTTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCAACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCGTa                                                              <  1:292570/150‑1 (MQ=255)                                                                                         tACCTTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCAACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCGTATCAAAc                                                        <  1:125741/150‑1 (MQ=255)                                                                                                  ccGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCAACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCGTATCAAACAGAGTTAACa                                              <  1:293619/151‑1 (MQ=255)                                                                                                   cGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCAACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCGTATCAAACAGAGTTAACat                                             <  2:318897/151‑1 (MQ=255)                                                                                                   cGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCAACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCGTATCAAACAGAGTTAACat                                             <  1:108843/151‑1 (MQ=255)                                                                                                                          cAACTCCTGTTCTGAAGGGTGAGCAACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCGTATCAAACAGAGTTAACATATCGCGCGCCGCCTGCCTTCCTg                      <  1:37958/151‑1 (MQ=255)                                                                                                                             cTCCTGTTCTGAAGGGTGAGCAACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCGTATCAAACAGAGTTAACATATCGCGCGCCGCCTGCCTTCCTGCgg                   >  1:292117/1‑151 (MQ=255)                                                                                                                                  gTTCTGAAGGGTGCGCAACAACACAGGGTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCGTATCAAACAGAGTTAACATATCGCTCGCCGCCTGCCTTCCTGCGGCTAtt              >  2:197488/1‑151 (MQ=255)                                                                                                                                    tCTGAAGGGTGAGCAACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCGTATCAAACAGAGTTAACATATCGCGCGCCGCCTGCCTTCCTGCGGCCATTg             >  2:81909/1‑150 (MQ=255)                                                                                                                                               aGCAACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCGTATCAAACAGAGTTAACATATCGCGCGCCGCCTGCCTTCCTGCGGCCATTGCAGTGACAAcc  <  2:155648/150‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                   TCAGCGCCCCCACGGGGCAAGCGCCAACACAAGGGGCGTTTTCACACTGATGGCACATCACCGGCGCACTGATGCTGTCCAGTCGCTGTACCTTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCGTATCAAACAGAGTTAACATATCGCGCGCCGCCTGCCTTCCTGCGGCCATTGCAGTGACAACC  >  NC_000913/3028796‑3029087

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑