Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,266,892	A→C	N58K (AAT→AAG)	tdcA ←	tdc operon transcriptional activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,266,892	0	A	C	100.0%	46.1 / NA	17	N58K (AAT→AAG)	tdcA	tdc operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (6/11);  total (6/11)

AAATGAGACTTCCACCACCGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCACGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTATTTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCCGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACTTCCTGAAAGACTACCAGGT  >  NC_000913/3266763‑3267034                                                                                                                                  |                                                                                                                                               aaaTGAGACTTCCACCACCGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCACGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTCTTTTTCCGCACCACTAATTcc                                                                                                                           <  1:431/151‑1 (MQ=255)         cTTCCACCACCGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCACGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTCTTTTTCCGCACCACTAATTCCACACCaaa                                                                                                                   >  2:242256/1‑151 (MQ=255)                     ccTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCACGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTCTTTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAat                                                                                                       >  1:187463/1‑151 (MQ=255)                       tCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCACGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAAGTGTTAATGTTACACCGGTCTTTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAatat                                                                                                     <  1:110904/151‑1 (MQ=255)                           aagaCATACCGCTTATCTCATTAACCATATTTTTCATTTCACGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTCTTTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAATATCGtt                                                                                                 >  2:314087/1‑151 (MQ=255)                              aCATACCGCTTATCTCATTAACCATATTTTTCATTTCACGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTCTTTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAATATCGTTa                                                                                                <  2:212276/149‑1 (MQ=255)                                             cATTAACCATATTTTTCATTTCACGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTCTTTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGc                                                                               <  2:143156/151‑1 (MQ=255)                                                  aCCATATTTTTCATTTCACGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTCTTTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCCGGtt                                                                          >  1:186082/1‑151 (MQ=255)                                                        tttttCATTTCACGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTCTTTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCCGGTTGAGTTa                                                                    <  2:168899/151‑1 (MQ=255)                                                                          aTGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTCTTTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCCGGTTGAGTTAACCCTAATTCTTTTGCag                                                  <  2:140405/151‑1 (MQ=255)                                                                                      cGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGTTCTTTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAATATCGTTAATAATTTTACTGACGGCCGGTTGATTTACCCCTAATTCTTTTGCAGCCGAGCCGATAg                                      <  2:300180/151‑1 (MQ=255)                                                                                             gTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTCTTTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCCGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCa                                 >  2:125932/1‑149 (MQ=255)                                                                                              tAACAATTGACCGGCAGGTGTTAATGTTACACCGTTCTTTTTCCGCACCACTAATCCCACCCCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCCGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACtt                              <  1:52400/151‑1 (MQ=255)                                                                                                               gttttAATTTTACACGGGTCTTTTTCGGCACCACTATTTCCACACCAAAATAATCTTCAATATCATTAATGATTTTACTGACGGCGGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACTTCCTGaa              <  1:314089/147‑1 (MQ=255)                                                                                                               gtataaaTGTTCCCCCGGTCTTTTCCCGCCCAACATATCCCCCACCAAAATAATCTCTAATATTGTTAAGGATTTTACGGGCGGCCGTTTGAGTTATCCCTAATTCTTTTCCAGCCGAGCCGATAGAACCACTTCTAATGACTTCCTGaa              <  2:63871/145‑1 (MQ=255)                                                                                                                     aTGTTACACCGGTCTTTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCCGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACTTCCTGAAAGACTa        >  1:280478/1‑150 (MQ=255)                                                                                                                          accccGGTCTTTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCCGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACTTCCTGAAAGACTACCAGGt  <  1:101577/148‑1 (MQ=255)                                                                                                                                  |                                                                                                                                               AAATGAGACTTCCACCACCGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCACGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTATTTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCCGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACTTCCTGAAAGACTACCAGGT  >  NC_000913/3266763‑3267034

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑